##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048258_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5056826
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.269813515434386	33.0	31.0	34.0	30.0	34.0
2	32.40121135273391	34.0	31.0	34.0	31.0	34.0
3	32.48121015039869	34.0	31.0	34.0	31.0	34.0
4	36.04708922157891	37.0	35.0	37.0	35.0	37.0
5	35.90611779009205	37.0	35.0	37.0	35.0	37.0
6	35.89545042680923	37.0	35.0	37.0	35.0	37.0
7	35.871118167799324	37.0	35.0	37.0	35.0	37.0
8	35.84057450266234	37.0	35.0	37.0	35.0	37.0
9	37.439871967119295	39.0	37.0	39.0	34.0	39.0
10-11	37.436756376430594	39.0	37.0	39.0	34.0	39.0
12-13	37.44151865221386	39.0	37.0	39.0	34.0	39.0
14-15	38.77450291942021	40.0	38.0	41.0	34.0	41.0
16-17	38.784202976333376	40.0	38.0	41.0	34.5	41.0
18-19	38.821046047461394	40.0	38.0	41.0	35.0	41.0
20-21	38.84584470575021	40.0	38.0	41.0	35.0	41.0
22-23	38.82503926375952	40.0	38.0	41.0	35.0	41.0
24-25	38.813789618230885	40.0	38.0	41.0	35.0	41.0
26-27	38.72490580059508	40.0	38.0	41.0	34.0	41.0
28-29	38.63064687216843	40.0	38.0	41.0	34.0	41.0
30-31	38.49853208316837	40.0	38.0	41.0	34.0	41.0
32-33	38.40241912614751	40.0	38.0	41.0	34.0	41.0
34-35	38.356252617748765	40.0	38.0	41.0	34.0	41.0
36-37	38.27524834748121	40.0	38.0	41.0	34.0	41.0
38-39	38.180615469070915	40.0	37.0	41.0	33.5	41.0
40-41	38.040663451738304	40.0	37.0	41.0	33.0	41.0
42-43	37.965191307749166	40.0	37.0	41.0	33.0	41.0
44-45	37.851585065414554	40.0	37.0	41.0	33.0	41.0
46-47	37.73624364374017	40.0	37.0	41.0	33.0	41.0
48-49	37.628471100251424	40.0	36.5	41.0	32.5	41.0
50-51	37.53159857586557	39.5	36.0	41.0	32.0	41.0
52-53	37.49707543822943	39.0	36.0	41.0	32.0	41.0
54-55	37.31298516104766	39.0	36.0	41.0	32.0	41.0
56-57	37.06933212651572	39.0	35.0	41.0	31.5	41.0
58-59	36.81565037041021	39.0	35.0	41.0	31.0	41.0
60-61	36.54168682094263	38.5	35.0	40.0	31.0	41.0
62-63	36.22170913138004	38.0	35.0	40.0	31.0	41.0
64-65	35.89489612654262	37.0	35.0	40.0	30.5	41.0
66-67	35.54813810085615	37.0	34.5	39.5	30.0	41.0
68-69	35.178966015441304	36.0	34.0	39.0	30.0	41.0
70-71	34.82003375239725	36.0	34.0	39.0	29.5	40.5
72-73	34.45310121408171	35.0	34.0	37.5	29.0	40.0
74-75	34.12430020332913	35.0	34.0	37.0	29.0	39.0
76-77	33.89541018417482	35.0	34.0	37.0	29.0	39.0
78-79	33.760913169644354	35.0	34.0	36.0	29.0	39.0
80-81	33.5335167751471	35.0	34.0	36.0	29.0	37.0
82-83	33.30884531126837	35.0	34.0	35.5	29.0	37.0
84-85	33.11531294531392	35.0	34.0	35.0	29.0	36.5
86-87	32.9265042934046	35.0	33.5	35.0	29.0	36.0
88-89	32.798996742225256	35.0	33.0	35.0	29.0	36.0
90-91	32.67442156404037	35.0	33.0	35.0	29.0	36.0
92-93	32.54519267619649	35.0	33.0	35.0	29.0	36.0
94-95	32.43861109715857	35.0	33.0	35.0	29.0	35.0
96-97	32.32934255598275	35.0	33.0	35.0	29.0	35.0
98-99	32.18734844742532	35.0	33.0	35.0	29.0	35.0
100-101	31.733569041133705	34.5	32.5	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30062237345823206
1101	2	0.33662370775700623
1101	3	0.33710364775061663
1101	4	0.22812240922664273
1101	5	0.23804037922612054
1101	6	0.2965682883515157
1101	7	0.27385068275584246
1101	8	0.2892081953790395
1101	9	0.40048342257619396
1101	10-11	0.43283448323062856
1101	12-13	0.37548572474040043
1101	14-15	0.47564402953778284
1101	16-17	0.45819546288188207
1101	18-19	0.4803703664110941
1101	20-21	0.46312440403918487
1101	22-23	0.5121991998870286
1101	24-25	0.5133182067586546
1101	26-27	0.507319006271409
1101	28-29	0.4457299572839304
1101	30-31	0.4450707045026334
1101	32-33	0.4916861331665814
1101	34-35	0.46442472641442833
1101	36-37	0.48251310030607186
1101	38-39	0.45213269350882257
1101	40-41	0.4710840664659912
1101	42-43	0.44324310388698507
1101	44-45	0.5151507332285234
1101	46-47	0.4808854292720497
1101	48-49	0.5098967230102716
1101	50-51	0.48000936799508054
1101	52-53	0.47638999765046464
1101	54-55	0.40461560169462985
1101	56-57	0.37488955368015553
1101	58-59	0.34106448369511355
1101	60-61	0.35580253922816496
1101	62-63	0.3417536594435475
1101	64-65	0.1959105366957985
1101	66-67	0.2987648927537734
1101	68-69	0.2607648446658217
1101	70-71	0.20772466098881637
1101	72-73	0.2240362189323406
1101	74-75	0.20165448243301398
1101	76-77	0.23179709995019948
1101	78-79	0.2302478396766503
1101	80-81	0.2222389181122253
1101	82-83	0.22356301697391956
1101	84-85	0.21093224073014483
1101	86-87	0.22031612776815024
1101	88-89	0.18633698976683633
1101	90-91	0.19678480757754357
1101	92-93	0.18567159927376053
1101	94-95	0.1411751527144176
1101	96-97	0.18455870276383024
1101	98-99	0.20759028218285636
1101	100-101	0.19950705997741736
1102	1	0.36147222701847426
1102	2	0.3616182279911442
1102	3	0.32676913804254326
1102	4	0.22027229165063744
1102	5	0.2603203598344379
1102	6	0.23282352566345565
1102	7	0.23333585147089764
1102	8	0.26518293851774644
1102	9	0.3034311081338572
1102	10-11	0.3929120260820582
1102	12-13	0.3649677900809536
1102	14-15	0.44979456249634353
1102	16-17	0.42220134267110154
1102	18-19	0.42003147663169216
1102	20-21	0.4376047740952558
1102	22-23	0.49319243401749446
1102	24-25	0.47433868979651805
1102	26-27	0.5015352810985192
1102	28-29	0.4952774363217216
1102	30-31	0.45353266022932814
1102	32-33	0.46181412692127566
1102	34-35	0.4581543118034901
1102	36-37	0.5080988900826426
1102	38-39	0.4905898266139843
1102	40-41	0.4445270001050403
1102	42-43	0.49981300331353395
1102	44-45	0.5092595862942417
1102	46-47	0.5111122685069347
1102	48-49	0.4926204460876491
1102	50-51	0.511415218740602
1102	52-53	0.48915424881424485
1102	54-55	0.46008712496869464
1102	56-57	0.48811481603592455
1102	58-59	0.42331971106571586
1102	60-61	0.3510272726972232
1102	62-63	0.27290758794172376
1102	64-65	0.3041880466125093
1102	66-67	0.3222644965163255
1102	68-69	0.3235267414410572
1102	70-71	0.32076118921582975
1102	72-73	0.2578745191703433
1102	74-75	0.27587923163362404
1102	76-77	0.220445161228362
1102	78-79	0.26843641392433426
1102	80-81	0.29603978829351263
1102	82-83	0.2602685402269387
1102	84-85	0.22700823380064605
1102	86-87	0.2930611932040321
1102	88-89	0.24784334626851034
1102	90-91	0.1766223870145467
1102	92-93	0.21725388217834052
1102	94-95	0.20291560296080746
1102	96-97	0.2021548576224177
1102	98-99	0.26271482042917427
1102	100-101	0.2759019354240273
1103	1	0.2897718626787693
1103	2	0.32579448318114146
1103	3	0.2860316980329074
1103	4	0.19625836515585604
1103	5	0.23123277427926325
1103	6	0.23607915734306317
1103	7	0.23539384204155311
1103	8	0.2616309717852161
1103	9	0.35638377410715094
1103	10-11	0.3461888424469919
1103	12-13	0.3483436058766287
1103	14-15	0.4772761676092827
1103	16-17	0.45356269776160474
1103	18-19	0.45623249535477584
1103	20-21	0.46864058167665945
1103	22-23	0.5208920190335888
1103	24-25	0.48457612460393307
1103	26-27	0.49087541399205037
1103	28-29	0.439975098883302
1103	30-31	0.4286031433268036
1103	32-33	0.41415416993300624
1103	34-35	0.47023629834516356
1103	36-37	0.4768199701424791
1103	38-39	0.4866182983717309
1103	40-41	0.4639113873468972
1103	42-43	0.41600081362193464
1103	44-45	0.4482439170389583
1103	46-47	0.40694097160506715
1103	48-49	0.4073072598493823
1103	50-51	0.455741053189449
1103	52-53	0.44722604889066986
1103	54-55	0.3951997091485069
1103	56-57	0.3430273594400788
1103	58-59	0.38505874441562327
1103	60-61	0.33765025569369556
1103	62-63	0.36646477637903985
1103	64-65	0.32750199637634125
1103	66-67	0.2813634476134226
1103	68-69	0.2685378333010533
1103	70-71	0.23132144341256833
1103	72-73	0.303548298817816
1103	74-75	0.24603543667424077
1103	76-77	0.2252246426775173
1103	78-79	0.21778430282068229
1103	80-81	0.1925246929351161
1103	82-83	0.1417863585551018
1103	84-85	0.1875958022528721
1103	86-87	0.1628268873829839
1103	88-89	0.1496194984023873
1103	90-91	0.14206037151026862
1103	92-93	0.173307830538441
1103	94-95	0.0936341966735128
1103	96-97	0.09760611282023746
1103	98-99	0.09497399139185347
1103	100-101	0.1321936843127105
1104	1	0.23266577659939713
1104	2	0.2482913276384764
1104	3	0.2106796730381859
1104	4	0.14316785635531204
1104	5	0.17745895607415463
1104	6	0.1662546489262482
1104	7	0.1747303676209384
1104	8	0.22668631093915792
1104	9	0.25020191455651997
1104	10-11	0.24025487809829826
1104	12-13	0.24953011820878146
1104	14-15	0.32570224061755937
1104	16-17	0.3147615914668549
1104	18-19	0.2749822804471478
1104	20-21	0.33061492774371715
1104	22-23	0.30393246787217265
1104	24-25	0.3024351186675531
1104	26-27	0.30365326786336055
1104	28-29	0.29436291552325855
1104	30-31	0.33614416874926434
1104	32-33	0.33217942040373316
1104	34-35	0.21978569537289872
1104	36-37	0.3792415719272242
1104	38-39	0.3111947194537308
1104	40-41	0.31717054537512723
1104	42-43	0.26229484307825146
1104	44-45	0.28090747390326953
1104	46-47	0.3470867033031624
1104	48-49	0.2653803232871468
1104	50-51	0.2541400844625272
1104	52-53	0.27621822586603884
1104	54-55	0.2960954373693525
1104	56-57	0.2895006786823231
1104	58-59	0.19778997954110622
1104	60-61	0.20972283504416112
1104	62-63	0.2079899278870201
1104	64-65	0.21787412998862266
1104	66-67	0.23166546501543905
1104	68-69	0.20700822855359036
1104	70-71	0.17707467689449885
1104	72-73	0.23006956944360013
1104	74-75	0.1792707665202542
1104	76-77	0.16153275997024252
1104	78-79	0.19240956876306825
1104	80-81	0.1570348529253991
1104	82-83	0.18030796623013146
1104	84-85	0.21079362526774048
1104	86-87	0.20688559302163156
1104	88-89	0.1828547827831386
1104	90-91	0.17811104252552923
1104	92-93	0.20899959530541423
1104	94-95	0.22790294548362766
1104	96-97	0.24293651250951598
1104	98-99	0.2735610862385798
1104	100-101	0.2779713865320659
1105	1	0.17574233486840285
1105	2	0.21338928647670485
1105	3	0.15281233717734466
1105	4	0.11463218454073854
1105	5	0.15295928237301837
1105	6	0.15117469819254126
1105	7	0.1759909514480782
1105	8	0.1709237950918805
1105	9	0.19863057989263666
1105	10-11	0.21277240879901171
1105	12-13	0.21816226868686073
1105	14-15	0.28086448741481007
1105	16-17	0.2307180962230504
1105	18-19	0.2292627294573748
1105	20-21	0.2518923593108511
1105	22-23	0.32207193067755213
1105	24-25	0.23574926008679142
1105	26-27	0.2820124034571734
1105	28-29	0.14996425165296046
1105	30-31	0.23273810592058197
1105	32-33	0.26776854001495565
1105	34-35	0.28236295050452753
1105	36-37	0.3007067371997536
1105	38-39	0.2849774734584045
1105	40-41	0.3271205060584492
1105	42-43	0.24550366837361537
1105	44-45	0.28157976876715196
1105	46-47	0.1898574837262288
1105	48-49	0.21456909507960376
1105	50-51	0.30448382346543923
1105	52-53	0.26456807854366104
1105	54-55	0.29572578168755115
1105	56-57	0.2883303582708052
1105	58-59	0.2773358817487974
1105	60-61	0.21771269106749003
1105	62-63	0.08972862350454847
1105	64-65	0.08597197949369928
1105	66-67	0.11846398066023767
1105	68-69	0.06351799405792491
1105	70-71	0.12372193172596724
1105	72-73	0.10463909286234241
1105	74-75	0.09712751677906795
1105	76-77	0.15734859578916627
1105	78-79	0.1046308406355223
1105	80-81	0.12403152178104193
1105	82-83	0.13853541299857142
1105	84-85	0.11473622130169048
1105	86-87	0.18631622486851285
1105	88-89	0.12591487173494897
1105	90-91	0.11437535460981962
1105	92-93	0.06126012837899708
1105	94-95	-0.024937759945359517
1105	96-97	0.09078025539241708
1105	98-99	0.10577751918624045
1105	100-101	0.04792339056646355
1106	1	0.14247659976408045
1106	2	0.15925147120532301
1106	3	0.14250261358944272
1106	4	0.1101022888773926
1106	5	0.11192358017399329
1106	6	0.1602942062969106
1106	7	0.12943571279861033
1106	8	0.19357681182159325
1106	9	0.2755300847959532
1106	10-11	0.22073197181566684
1106	12-13	0.23509609522648844
1106	14-15	0.29790688991383973
1106	16-17	0.28324956895220765
1106	18-19	0.27280595466164925
1106	20-21	0.25486426266390083
1106	22-23	0.29246433291968543
1106	24-25	0.29930919047797033
1106	26-27	0.25083321715838025
1106	28-29	0.2967564282509798
1106	30-31	0.3207509419256098
1106	32-33	0.32345404742192585
1106	34-35	0.2617068831781282
1106	36-37	0.25890252078809084
1106	38-39	0.22262944950693253
1106	40-41	0.32888738591207556
1106	42-43	0.25427389149430013
1106	44-45	0.23713507227137143
1106	46-47	0.210721929646148
1106	48-49	0.20415508525211834
1106	50-51	0.22719926167751936
1106	52-53	0.12610181188246372
1106	54-55	0.17959884902334977
1106	56-57	0.2246438205555421
1106	58-59	0.20948344442693667
1106	60-61	0.11869771737630685
1106	62-63	0.1930161096695997
1106	64-65	0.14838495513517103
1106	66-67	0.20295260582587815
1106	68-69	0.20518121683087998
1106	70-71	0.2072657036877672
1106	72-73	0.18966630810515994
1106	74-75	0.18201566754532195
1106	76-77	0.21811070789791387
1106	78-79	0.23692504871883102
1106	80-81	0.21631577965320758
1106	82-83	0.23844160627793087
1106	84-85	0.24473065484491485
1106	86-87	0.2754127119612022
1106	88-89	0.2797884548065639
1106	90-91	0.23580823445433907
1106	92-93	0.3065925012028572
1106	94-95	0.2821268802673558
1106	96-97	0.3362004341763054
1106	98-99	0.3955319765929062
1106	100-101	0.40736866047902964
1107	1	0.03423274535474263
1107	2	0.09425945791608825
1107	3	0.06702091683772693
1107	4	0.05621366171416042
1107	5	0.11035560039390901
1107	6	0.09254256603772859
1107	7	0.10991976459673936
1107	8	0.03920093885426468
1107	9	0.14939044558780523
1107	10-11	0.01763772039167577
1107	12-13	0.10606910757191201
1107	14-15	0.04895643727058285
1107	16-17	-0.001368414221992964
1107	18-19	0.019096278078500006
1107	20-21	0.0579980563856779
1107	22-23	0.13841648514042504
1107	24-25	0.10773097311414404
1107	26-27	0.0377489271374003
1107	28-29	0.08521322672805098
1107	30-31	0.11849848453484668
1107	32-33	0.12431252374056356
1107	34-35	0.09069705642533421
1107	36-37	0.038716994199369026
1107	38-39	0.08985112800842643
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2216	54-55	-1.1029941930772083
2216	56-57	-1.2444083439223945
2216	58-59	-0.953893278609705
2216	60-61	-0.9482156610418002
2216	62-63	-0.8633751614452692
2216	64-65	-0.9319747286245672
2216	66-67	-0.6714026540666893
2216	68-69	-0.6083776858524459
2216	70-71	-0.6155145256043824
2216	72-73	-0.5518017979943011
2216	74-75	-0.5341675598308981
2216	76-77	-0.525754046880408
2216	78-79	-0.42010408353549167
2216	80-81	-0.4380004416981649
2216	82-83	-0.6700960126683668
2216	84-85	-0.49635549504540677
2216	86-87	-0.49954431756736284
2216	88-89	-0.4905078749632068
2216	90-91	-0.49302739978396914
2216	92-93	-0.4368046763384399
2216	94-95	-0.5877969522721429
2216	96-97	-0.6140678583078625
2216	98-99	-0.8547328764072759
2216	100-101	-0.6798728641294716
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	26.0
13	218.0
14	707.0
15	1640.0
16	2894.0
17	4897.0
18	8692.0
19	18176.0
20	20271.0
21	18672.0
22	17961.0
23	18288.0
24	19758.0
25	23715.0
26	30414.0
27	40126.0
28	52021.0
29	68365.0
30	90153.0
31	116743.0
32	152977.0
33	202303.0
34	273218.0
35	389326.0
36	649417.0
37	1216442.0
38	1336231.0
39	282842.0
40	329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.117836017231365	39.994055557401246	13.686574814547628	20.201533610819755
2	24.17059633849375	28.231602194736382	30.116816358719877	17.48098510804999
3	22.2608213136066	27.595135763026057	25.204663953238654	24.939378970128693
4	23.580792764654017	26.722140659630234	26.142704443214797	23.554362132500952
5	23.51162567191357	27.49357007735682	25.449758405766783	23.54504584496283
6	23.631780092888306	26.164495278263477	26.21549564885167	23.988228979996542
7	24.2405413988933	27.590449028699034	24.860337294579644	23.30867227782803
8	24.016468037460655	25.78647159305066	26.53520212085605	23.66185824863264
9	23.255773483208635	25.67472165346405	26.725202725978708	24.344302137348606
10-11	24.203789096164275	26.292648787994683	25.663360772152334	23.840201343688708
12-13	23.91083853784963	26.43866923639453	25.57103012838488	24.07946209737096
14-15	23.555882682140933	26.304593039190987	25.993310823825063	24.146213454843018
16-17	23.76546078508535	26.713100272779805	25.79270079690304	23.728738145231812
18-19	23.172420803088738	26.701067032957038	26.5115608090925	23.614951354861727
20-21	22.623894909573714	26.72746699214092	26.85364297683962	23.794995121445744
22-23	22.46516119648582	26.74248893895983	27.432457378645953	23.3598924859084
24-25	22.334632435444686	26.360764637739166	28.388973636822783	22.915629289993365
26-27	22.327850675428955	26.11596983758678	28.95920729683367	22.596972190150595
28-29	22.331524554957465	26.393375678786274	28.840397720306438	22.434702045949827
30-31	22.413288493612395	26.28158453543784	28.746243196819503	22.558883774130255
32-33	22.356909254935804	26.179277277881425	28.882909951815627	22.580903515367147
34-35	22.371117772294323	26.447924053546636	28.76292362046865	22.418034553690397
36-37	22.360201834114918	26.454281796526118	28.55799270135061	22.62752366800835
38-39	22.371239808077124	26.33526030078569	28.75411102979084	22.539388861346342
40-41	22.516151946054645	26.62852808117986	28.186977549729363	22.668342423036133
42-43	22.606273268072318	26.68547558130383	27.979460222250257	22.72879092837359
44-45	22.51956914364838	26.687016110740103	28.11101577218695	22.68239897342457
46-47	22.64565347112737	26.94267991411603	27.819010851181748	22.592655763574854
48-49	22.686859306608532	27.015335311122037	27.570673778374022	22.72713160389541
50-51	22.588822875722574	26.767684707749606	27.926753169655356	22.716739246872468
52-53	22.62598658804845	27.041008582869836	27.679243531206797	22.653761297874915
54-55	22.686072837112732	26.938979802647	27.55555836806313	22.81938899217713
56-57	22.67605793954603	26.893034113813563	27.688583097389714	22.742324849250693
58-59	22.84143916316575	27.058833184490027	27.395075356373592	22.704652295970632
60-61	22.720311817133727	27.052717029115193	27.332735459706814	22.89423569404427
62-63	22.729511832461935	26.96833121440814	27.468597291295755	22.833559661834173
64-65	22.900739734105464	27.200430626617493	27.20360455994354	22.6952250793335
66-67	22.835080414236945	27.09139505965809	27.116292149051898	22.95723237705307
68-69	22.78769820866668	27.099878737349687	27.1424155867989	22.97000746718473
70-71	22.8706496877031	27.321215474628108	26.954037098314913	22.85409773935388
72-73	22.85093596800442	27.21545452397142	26.85722568723231	23.076383820791847
74-75	22.810015251777934	27.169334285848475	27.042356436769392	22.9782940256042
76-77	22.934504484960712	27.339552826902608	26.811628881489813	22.914313806646867
78-79	22.88410586728186	27.30203871126965	26.77018293307536	23.043672488373133
80-81	22.812948558339617	27.22446127516166	26.92956282553155	23.033027340967173
82-83	22.88630330016383	27.463316763579275	26.708694705948254	22.94168523030864
84-85	22.918931756797644	27.287818485350297	26.710826118992426	23.082423638859634
86-87	22.870032085349997	27.219994759557625	26.921783925684593	22.98818922940778
88-89	22.934273899669783	27.381424045567055	26.663184894275773	23.021117160487396
90-91	22.982921952727743	27.3935255868147	26.5377594012514	23.085793059206157
92-93	22.834772729632284	27.28030481682366	26.800159982360412	23.084762471183648
94-95	23.003955445570007	27.483039756558757	26.52285247702808	22.990152320843155
96-97	22.9348622582327	27.460550887967777	26.461984502252854	23.142602351546664
98-99	22.936102661305593	27.283862544117376	26.685304852998105	23.094729941578926
100-101	23.031641509090665	27.512916314307663	26.338008336372386	23.117433840229285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2381.0
1	1759.5
2	810.0
3	386.0
4	246.5
5	200.5
6	184.0
7	150.5
8	135.0
9	137.0
10	147.0
11	162.5
12	183.0
13	327.0
14	467.0
15	597.5
16	3346.0
17	3939.0
18	1954.5
19	2189.5
20	2781.5
21	3701.5
22	4870.5
23	6477.0
24	8249.5
25	10990.5
26	15093.5
27	21196.0
28	28268.5
29	36375.5
30	47418.5
31	57661.5
32	69137.5
33	88608.0
34	108595.0
35	127182.5
36	144596.5
37	155533.5
38	161894.5
39	170317.0
40	175352.5
41	173031.0
42	170384.5
43	173669.0
44	179102.0
45	184741.5
46	188204.0
47	191238.0
48	195045.0
49	190601.0
50	181992.0
51	175806.5
52	176603.0
53	175834.0
54	166010.0
55	155403.0
56	145167.0
57	130002.0
58	115616.5
59	104645.5
60	91975.0
61	78148.5
62	63708.0
63	49034.5
64	36853.0
65	26209.0
66	18541.0
67	13981.0
68	10576.0
69	8109.5
70	6284.0
71	4701.0
72	3778.0
73	2868.5
74	2010.5
75	1517.0
76	1111.0
77	746.5
78	447.5
79	208.0
80	71.0
81	20.5
82	10.5
83	3.5
84	0.5
85	1.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.955050065001248E-5
2	0.0
3	0.0
4	0.041231396927638005
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.88762516250312E-6
24-25	0.0
26-27	4.9438125812515594E-5
28-29	1.1865150195003743E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	5.9325750975018714E-5
40-41	9.887625162503119E-5
42-43	7.910100130002496E-5
44-45	2.570782542250811E-4
46-47	6.921337613752185E-5
48-49	0.0
50-51	1.2853912711254054E-4
52-53	0.0013348293969379212
54-55	9.788748910878088E-4
56-57	6.921337613752185E-5
58-59	0.0010678635175503368
60-61	3.1640400520009985E-4
62-63	3.3617925552510605E-4
64-65	1.7797725292505615E-4
66-67	1.9775250325006238E-4
68-69	6.723585110502121E-4
70-71	3.856173813376217E-4
72-73	4.9438125812515594E-5
74-75	7.613471375127402E-4
76-77	7.316842620252309E-4
78-79	7.910100130002496E-5
80-81	9.88762516250312E-6
82-83	0.0024224681648132643
84-85	0.0
86-87	1.977525032500624E-5
88-89	2.8674112971259047E-4
90-91	2.47190629062578E-4
92-93	3.6584213101261543E-4
94-95	0.0
96-97	5.240441336126653E-4
98-99	6.921337613752185E-5
100-101	0.008503357639752683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5056826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.12353683906234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71546743399131	39.32437307380879
2	9.248823102228048	8.901721585560844
3	2.877110831621721	4.153702472868395
4	1.3721869665701298	2.641379601432754
5	0.8279652136315563	1.9922307229830158
6	0.583923983901524	1.686029241029822
7	0.4321145259525175	1.4556415515858951
8	0.3569043698695382	1.374040047315124
9	0.27816521620860474	1.2047664626604477
>10	1.8940183863015756	17.743688176420193
>50	0.290585872196922	9.892262253531264
>100	0.12219799002267835	8.374529203945693
>500	1.6495642566695913E-4	0.05118207721225592
>1k	2.0619504339082048E-4	0.2262207870100914
>5k	8.247801735632819E-5	0.22177535703463208
>10k+	8.247801735632819E-5	0.7564573856008118
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23867	0.47197589950692387	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	14250	0.2817973171313389	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5893	0.11653555016526178	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	5282	0.10445287221668295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.955050065001248E-5	0.0	0.0	0.0	0.0
2	7.910100130002496E-5	0.0	0.0	0.0	0.0
3	1.1865150195003743E-4	0.0	0.0	0.0	0.0
4	1.1865150195003743E-4	0.0	0.0	0.0	0.0
5	1.5820200260004992E-4	0.0	0.0	1.977525032500624E-5	0.0
6	3.955050065001248E-4	0.0	0.0	1.977525032500624E-5	3.955050065001248E-5
7	6.328080104001997E-4	0.0	0.0	1.977525032500624E-5	3.955050065001248E-5
8	0.0011667397691753681	0.0	0.0	3.955050065001248E-5	3.955050065001248E-5
9	0.002906961797775917	0.0	0.0	3.955050065001248E-5	3.955050065001248E-5
10-11	0.010629197049690852	1.977525032500624E-5	0.0	3.955050065001248E-5	3.955050065001248E-5
12-13	0.01789660154413065	1.977525032500624E-5	0.0	3.955050065001248E-5	3.955050065001248E-5
14-15	0.024768501032070317	1.977525032500624E-5	0.0	3.955050065001248E-5	3.955050065001248E-5
16-17	0.03129433363932237	2.966287548750936E-5	0.0	5.932575097501872E-5	5.9325750975018714E-5
18-19	0.03601073084183636	3.955050065001248E-5	0.0	7.910100130002496E-5	5.9325750975018714E-5
20-21	0.04253656344908842	3.955050065001248E-5	0.0	7.910100130002496E-5	5.9325750975018714E-5
22-23	0.05058509033136596	3.955050065001248E-5	0.0	7.910100130002496E-5	7.910100130002496E-5
24-25	0.05839631420974342	3.955050065001248E-5	0.0	7.910100130002496E-5	7.910100130002496E-5
26-27	0.0691144998858968	3.955050065001248E-5	0.0	7.910100130002496E-5	7.910100130002496E-5
28-29	0.07776617190308704	3.955050065001248E-5	0.0	7.910100130002496E-5	8.898862646252809E-5
30-31	0.08593335028731461	3.955050065001248E-5	0.0	7.910100130002496E-5	9.88762516250312E-5
32-33	0.09532659419169257	3.955050065001248E-5	0.0	7.910100130002496E-5	9.88762516250312E-5
34-35	0.10505601735159564	3.955050065001248E-5	0.0	7.910100130002496E-5	1.0876387678753432E-4
36-37	0.11528970939478637	3.955050065001248E-5	0.0	7.910100130002496E-5	1.1865150195003743E-4
38-39	0.12657148970520243	3.955050065001248E-5	0.0	7.910100130002496E-5	1.1865150195003743E-4
40-41	0.13996922180039417	3.955050065001248E-5	0.0	7.910100130002496E-5	1.3842675227504367E-4
42-43	0.15543346755454907	4.9438125812515594E-5	0.0	7.910100130002496E-5	1.3842675227504367E-4
44-45	0.17454624699366755	5.9325750975018714E-5	0.0	7.910100130002496E-5	1.5820200260004992E-4
46-47	0.19590351734467432	5.9325750975018714E-5	0.0	7.910100130002496E-5	1.6808962776255305E-4
48-49	0.2193273013546442	5.9325750975018714E-5	0.0	7.910100130002496E-5	1.7797725292505615E-4
50-51	0.24774433607167817	5.9325750975018714E-5	0.0	7.910100130002496E-5	1.7797725292505615E-4
52-53	0.280660240237651	5.9325750975018714E-5	0.0	7.910100130002496E-5	1.8786487808755928E-4
54-55	0.3163941175749373	5.9325750975018714E-5	0.0	9.88762516250312E-5	1.977525032500624E-4
56-57	0.3563302356062874	5.9325750975018714E-5	0.0	9.88762516250312E-5	1.977525032500624E-4
58-59	0.4029405006223271	5.9325750975018714E-5	0.0	9.88762516250312E-5	2.1752775357506863E-4
60-61	0.4551965996061561	5.9325750975018714E-5	0.0	9.88762516250312E-5	2.3730300390007486E-4
62-63	0.5140180816978872	7.910100130002496E-5	0.0	9.88762516250312E-5	2.3730300390007486E-4
64-65	0.5802651702866581	7.910100130002496E-5	0.0	1.0876387678753432E-4	2.3730300390007486E-4
66-67	0.6516340487096055	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.3730300390007486E-4
68-69	0.7331278552989563	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.3730300390007486E-4
70-71	0.8233227720313098	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.3730300390007486E-4
72-73	0.9209630705110281	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.570782542250811E-4
74-75	1.027739534640899	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.570782542250811E-4
76-77	1.1471721589787744	7.910100130002496E-5	0.0	1.3842675227504367E-4	2.570782542250811E-4
78-79	1.2771746546153655	9.88762516250312E-5	0.0	1.3842675227504367E-4	2.570782542250811E-4
80-81	1.4194773559541103	9.88762516250312E-5	0.0	1.3842675227504367E-4	2.8674112971259047E-4
82-83	1.5738330723659466	9.88762516250312E-5	0.0	1.483143774375468E-4	3.1640400520009985E-4
84-85	1.7423577556356498	9.88762516250312E-5	0.0	1.7797725292505615E-4	3.1640400520009985E-4
86-87	1.9229947797294193	9.88762516250312E-5	0.0	1.8786487808755928E-4	3.1640400520009985E-4
88-89	2.120243014096194	9.88762516250312E-5	0.0	1.977525032500624E-4	3.26291630362603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	4570	0.0	71.33032	1
TCGACGG	5300	0.0	63.388405	2
ATATCTC	3665	0.0	63.27208	1
TATCTCG	4095	0.0	56.39604	2
CGACGGC	6015	0.0	55.616455	3
ATCTCGA	8575	0.0	47.10327	1
ACGGCGC	7260	0.0	46.32103	5
GACGGCG	7280	0.0	46.08288	4
CGGCGCG	7395	0.0	45.411186	6
TCTCGAG	8250	0.0	44.00537	2
GGTCGAC	505	0.0	38.57969	1
TCGAGGG	9465	0.0	38.356503	4
CTCGAGG	9555	0.0	37.995216	3
CGAGGGC	9590	0.0	37.840454	5
GGGCGCG	9860	0.0	36.756084	8
AGGGCGC	9990	0.0	36.277775	7
GAGGGCG	10835	0.0	33.53622	6
CTCGACG	1370	0.0	29.13567	3
CGCGCCG	16905	0.0	25.287678	9
GGCGCGC	17560	0.0	24.398527	7
>>END_MODULE