FastQCFastQC Report
Tue 31 May 2016
SRR2048257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702900
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT46940.27564742498091493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG79100.091.125861
TCGACGG84850.085.1060642
TATCTCG22600.083.6649252
CGACGGC88700.081.465633
ACGGCGC90150.080.313415
GACGGCG90800.079.686154
ATATCTC23750.079.4254151
CGGCGCG91400.079.3126
ATCTCGA25150.075.3708953
TCTCGAG19500.072.845954
AGGGCGC20300.070.420489
CTCGAGG20450.069.461915
TCGAGGG20700.068.846436
CGAGGGC20650.068.777037
CGCGCCG109450.066.607199
GCGCGCC112600.064.791738
GGCGCGC112650.064.682457
CTCGACG7750.061.9139445
GAGGGCG23250.061.89948
TCTCGAC9250.051.8738484
Produced by FastQC (version 0.11.5)