##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048257_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1702900
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.407779082741207	31.0	31.0	34.0	28.0	34.0
2	31.596565858241824	31.0	31.0	34.0	28.0	34.0
3	31.551223794703155	31.0	31.0	34.0	28.0	34.0
4	35.301537377414995	37.0	35.0	37.0	32.0	37.0
5	35.24426918785601	37.0	35.0	37.0	32.0	37.0
6	35.25264020200834	37.0	35.0	37.0	32.0	37.0
7	35.1982265547008	37.0	35.0	37.0	32.0	37.0
8	35.23371190322391	37.0	35.0	37.0	32.0	37.0
9	36.815041987198306	39.0	37.0	39.0	32.0	39.0
10-11	36.79013154031358	39.0	37.0	39.0	32.0	39.0
12-13	36.802315168242416	39.0	37.0	39.0	32.0	39.0
14-15	37.95829203124082	40.0	38.0	41.0	32.0	41.0
16-17	37.94873597979917	40.0	38.0	41.0	32.0	41.0
18-19	37.9854556932292	40.0	38.0	41.0	32.0	41.0
20-21	37.91048916554114	40.0	38.0	41.0	32.0	41.0
22-23	38.01607933525163	40.0	38.0	41.0	32.0	41.0
24-25	38.0074076575254	40.0	38.0	41.0	32.0	41.0
26-27	38.05038346350344	40.0	38.0	41.0	32.0	41.0
28-29	37.824319396323915	40.0	37.5	41.0	32.0	41.0
30-31	37.64385518820835	40.0	36.5	41.0	32.0	41.0
32-33	37.54200951318339	40.0	36.0	41.0	32.0	41.0
34-35	37.29880057548887	40.0	35.5	41.0	31.5	41.0
36-37	37.1937192436432	40.0	35.0	41.0	31.0	41.0
38-39	37.04442568559516	39.5	35.0	41.0	30.5	41.0
40-41	36.59479182570908	39.5	35.0	41.0	29.5	41.0
42-43	36.43076986317458	39.0	35.0	40.5	29.0	41.0
44-45	36.24283281461037	39.0	35.0	40.0	28.5	41.0
46-47	36.15654090081625	39.0	35.0	40.0	28.0	41.0
48-49	36.02731047037406	38.5	34.0	40.0	28.0	41.0
50-51	35.984546655704975	38.0	34.0	40.0	28.0	41.0
52-53	35.83215191731752	38.0	34.0	40.0	28.0	41.0
54-55	35.964274179341125	38.0	34.0	40.0	28.0	41.0
56-57	35.858710435140054	38.0	34.0	40.0	28.0	41.0
58-59	35.62087174819426	38.0	34.0	40.0	28.0	41.0
60-61	35.361752598508424	37.5	34.0	40.0	28.0	41.0
62-63	35.0350287744436	37.0	34.0	40.0	27.5	41.0
64-65	34.67213841094603	36.5	33.0	40.0	27.0	41.0
66-67	34.33627664572201	36.0	33.0	39.0	26.0	41.0
68-69	33.9982450525574	35.5	33.0	39.0	26.0	41.0
70-71	33.61994949791532	35.0	32.5	38.5	26.0	40.0
72-73	33.42601591402901	35.0	32.5	37.5	26.0	40.0
74-75	33.3676272828704	35.0	33.0	37.0	26.5	39.0
76-77	33.04243114686711	35.0	33.0	37.0	26.0	39.0
78-79	32.738557754418935	35.0	33.0	36.0	26.0	39.0
80-81	32.48447941746433	35.0	33.0	36.0	26.0	37.0
82-83	32.22872071172705	35.0	32.0	35.5	26.0	37.0
84-85	31.997616712666627	35.0	32.0	35.0	25.5	36.5
86-87	31.79817311644841	35.0	32.0	35.0	25.0	36.0
88-89	31.616879147336896	35.0	32.0	35.0	25.0	36.0
90-91	31.430673263256796	35.0	32.0	35.0	25.0	36.0
92-93	31.275933114099477	35.0	32.0	35.0	24.5	35.5
94-95	31.144881966057902	35.0	32.0	35.0	24.0	35.0
96-97	30.99039931880909	35.0	32.0	35.0	24.0	35.0
98-99	30.816619296494217	35.0	32.0	35.0	23.0	35.0
100-101	30.208511362969055	34.5	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	27.0
11	266.0
12	1072.0
13	2273.0
14	3189.0
15	3836.0
16	4275.0
17	5187.0
18	7012.0
19	10773.0
20	10709.0
21	10823.0
22	12378.0
23	14371.0
24	16032.0
25	18115.0
26	21242.0
27	25027.0
28	29879.0
29	34728.0
30	39926.0
31	47519.0
32	57473.0
33	71916.0
34	94586.0
35	133197.0
36	219045.0
37	368517.0
38	365876.0
39	73541.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.131407789521923	38.537334829585845	13.821260494820892	19.509996886071338
2	23.174602055758893	27.79897288416546	32.159780361916475	16.86664469815917
3	21.83285733921835	27.803362543042127	23.503673045496893	26.860107072242634
4	26.02983229905611	26.22873779016352	25.259058835139893	22.48237107564048
5	22.453345928331874	30.36910277671547	24.58867989259004	22.58887140236262
6	21.969716549345414	25.37757394058438	26.085789404434085	26.56692010563612
7	26.710406687042916	25.976896999711034	24.130994528484067	23.181701784761984
8	26.902005697001734	25.084662144304463	25.598860599653484	22.41447155904032
9	21.35374977023287	25.963304206984805	25.936466231425566	26.746479791356766
10-11	25.1042431613227	25.65613512955632	24.972671129525782	24.266950579595207
12-13	24.687986855310346	25.69650588733408	24.85951847826023	24.75598877909534
14-15	24.55633315862956	25.567207367507738	25.135879897761587	24.74057957610111
16-17	24.660432203887485	27.74625638616478	24.990428093252685	22.60288331669505
18-19	22.27163795386573	25.467805195727987	27.342578743660077	24.9179781067462
20-21	22.258706374161783	28.171781821769663	25.341131062219347	24.228380741849207
22-23	21.78637726982117	27.864128051819364	28.02647472481387	22.323019953545593
24-25	21.72528793760378	25.65853117021108	30.16844254375377	22.447738348431372
26-27	21.653414763051266	25.45751365317987	31.067502495742556	21.82156908802631
28-29	21.714681564088316	25.49874725871075	31.084370040865327	21.702201136335606
30-31	21.73327852486934	25.419108579482057	31.006400845616298	21.841212050032297
32-33	21.73857504680101	25.359527218000956	31.125000917666924	21.776896817531107
34-35	21.774307207159595	25.53127330596809	31.03065964331225	21.663759843560065
36-37	21.768650753917136	25.44323151221234	30.92145232003754	21.866665413832987
38-39	21.89782801119033	25.4471111121553	30.659496599494517	21.995564277159847
40-41	22.60118033942099	26.253919783898056	28.42947325151213	22.71542662516883
42-43	22.658168230050922	26.433575304068597	28.382074942610007	22.526181523270473
44-45	22.784454795281576	26.6963312797724	28.02275291877066	22.496461006175362
46-47	22.661929649421573	27.15332667801985	27.785924011979564	22.39881966057901
48-49	22.676845381408185	27.244259792119323	27.526278701039402	22.552616125433083
50-51	22.60385225204064	27.183216865347347	27.716454283868696	22.49647659874332
52-53	22.617300147609775	27.289661942194638	27.594395233029356	22.498642677166234
54-55	22.68917235680782	27.03169898502407	27.68142335061273	22.597705307555383
56-57	22.619942451112806	26.85442480474485	28.030271889130308	22.495360855012038
58-59	22.677050913148157	27.004727230019377	27.87488989371073	22.443331963121736
60-61	22.6946972811087	26.942333666099007	27.797844852897995	22.5651241998943
62-63	22.711961947266428	26.914528157848377	27.85471842151624	22.51879147336896
64-65	22.866855314462097	27.14032395076287	27.53311344325488	22.459707291520157
66-67	22.920785887059125	27.080770290053795	27.31190663407112	22.68653718881596
68-69	22.882553291444008	27.132773504022552	27.311644841153328	22.673028363380116
70-71	22.944124728404486	27.400405191144518	27.032033589758647	22.62343649069235
72-73	22.903562617852025	27.297215242204807	26.961195670620956	22.838026469322212
74-75	22.911544268384993	27.242311190870616	27.144389825103925	22.701754715640465
76-77	23.001382963417534	27.39756234346234	26.929498227986365	22.67155646513376
78-79	23.027981678313466	27.29511421692407	26.912472840448647	22.764431264313817
80-81	23.04636208820248	27.139585413118798	27.011627224147045	22.802425274531682
82-83	23.110928416231136	27.367578836103117	26.855599271830403	22.66589347583534
84-85	23.17205942803453	27.21545598684597	26.840624816489516	22.771859768629984
86-87	23.199747489576605	27.177814316753775	26.94597451406424	22.67646367960538
88-89	23.281937659856027	27.24702778995472	26.757832743506004	22.713201806683248
90-91	23.39538434435375	27.243202771742318	26.580216101943744	22.781196781960187
92-93	23.301250807446124	27.193258558928886	26.69490281284867	22.810587820776323
94-95	23.43496388513712	27.397997533619122	26.53432380057549	22.632714780668273
96-97	23.423454107698632	27.33862822244407	26.48452639614775	22.753391273709557
98-99	23.483704269187854	27.26293381877973	26.616125433084736	22.637236478947678
100-101	23.618533090610136	27.45026131892654	26.306858887779672	22.624346702683656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	621.0
1	495.0
2	306.5
3	208.0
4	147.5
5	100.5
6	71.0
7	66.0
8	62.0
9	51.5
10	42.5
11	45.0
12	50.0
13	55.0
14	63.5
15	107.0
16	876.0
17	1022.5
18	484.5
19	653.5
20	950.0
21	1342.0
22	1878.0
23	2644.0
24	3494.5
25	4560.5
26	6195.0
27	8532.5
28	11188.0
29	14187.5
30	18114.0
31	22222.5
32	26394.5
33	32145.0
34	38116.0
35	43392.5
36	48105.5
37	51379.0
38	53891.5
39	56750.0
40	58022.0
41	57306.0
42	56751.0
43	57469.5
44	58845.0
45	60572.5
46	61995.5
47	63171.5
48	64396.5
49	63437.5
50	60617.0
51	58200.0
52	57970.5
53	57733.5
54	54867.5
55	51350.5
56	47857.5
57	42969.0
58	38692.0
59	35361.5
60	30889.0
61	26250.0
62	21687.0
63	16759.5
64	12581.0
65	9299.5
66	6742.5
67	4853.5
68	3559.0
69	2755.0
70	2222.5
71	1794.0
72	1441.5
73	1108.5
74	809.0
75	618.5
76	474.5
77	333.0
78	223.5
79	123.0
80	51.0
81	14.0
82	4.5
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	1.0
99	2.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05108931822185683
2	0.027188913030712317
3	0.029244230430442185
4	0.03429443889834988
5	0.03740677667508368
6	0.026542956133654358
7	0.015502965529391038
8	0.01468083856949909
9	0.005226378530741677
10-11	0.001791062305478889
12-13	4.1106347994597454E-4
14-15	0.0010276586998649363
16-17	0.0
18-19	5.872335427799636E-5
20-21	0.010687650478595337
22-23	0.021757002759997653
24-25	0.021199130894356686
26-27	0.0
28-29	0.013741264901051148
30-31	0.0
32-33	0.012478712784074227
34-35	5.872335427799636E-4
36-37	0.00549063362499266
38-39	0.021081684185800694
40-41	0.0
42-43	0.00452169827940572
44-45	0.005020846790768688
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.005842973750660637
54-55	0.005931058782077632
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.004668506665100711
66-67	5.285101885019672E-4
68-69	0.0
70-71	0.0
72-73	0.0013506371483939163
74-75	1.7617006283398909E-4
76-77	0.0020553173997298725
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.936167713899818E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1702900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.16181215484475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50156301871407	58.96103289582055
2	6.998706655093502	9.540890567026903
3	2.20976496654867	4.518647536687418
4	1.168460027899949	3.18577411728644
5	0.7136484071429587	2.4321784336141263
6	0.4745410338581675	1.9407346085763741
7	0.3363221430806725	1.6047028718125733
8	0.2415992072570026	1.3174271825448975
9	0.19037234786860763	1.1678511793407116
>10	1.1507419138972306	14.14331801290264
>50	0.012635648461727317	0.5180595224225518
>100	0.0012984155487540986	0.23381160818290603
>500	1.731073143414526E-4	0.06831799472564846
>1k	1.7310731434145253E-4	0.3672534690562708
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	4694	0.27564742498091493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.872335427799636E-5	0.0	0.0	0.0	0.0
2	5.872335427799636E-5	0.0	0.0	0.0	0.0
3	1.1744670855599271E-4	0.0	0.0	0.0	0.0
4	1.7617006283398909E-4	0.0	0.0	0.0	0.0
5	1.7617006283398909E-4	0.0	0.0	0.0	0.0
6	1.7617006283398909E-4	0.0	0.0	0.0	0.0
7	2.3489341711198543E-4	0.0	0.0	0.0	0.0
8	3.5234012566797817E-4	0.0	0.0	0.0	0.0
9	4.6978683422397086E-4	0.0	0.0	0.0	5.872335427799636E-5
10-11	0.0015855305655059016	0.0	0.0	0.0	5.872335427799636E-5
12-13	0.0030242527453168125	0.0	0.0	0.0	5.872335427799636E-5
14-15	0.004697868342239709	0.0	0.0	0.0	5.872335427799636E-5
16-17	0.005872335427799636	0.0	0.0	0.0	1.1744670855599271E-4
18-19	0.006988079159081567	2.936167713899818E-5	0.0	0.0	1.1744670855599271E-4
20-21	0.008602971401726466	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
22-23	0.00983616184156439	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
24-25	0.011216160667097304	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
26-27	0.013418286452522167	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
28-29	0.015591050560808034	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
30-31	0.01752892125198191	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
32-33	0.01981913206882377	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
34-35	0.0225497680427506	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
36-37	0.026689764519349346	5.872335427799636E-5	0.0	0.0	1.1744670855599271E-4
38-39	0.03182805801867403	8.808503141699453E-5	0.0	0.0	1.1744670855599271E-4
40-41	0.038375712020670624	1.1744670855599271E-4	0.0	0.0	1.7617006283398909E-4
42-43	0.046127194785366143	1.1744670855599271E-4	0.0	0.0	1.7617006283398909E-4
44-45	0.056227611721181514	1.1744670855599271E-4	0.0	0.0	2.0553173997298724E-4
46-47	0.06826589934817076	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
48-49	0.08306418462622585	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
50-51	0.10270714663221563	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
52-53	0.12496329790357624	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
54-55	0.1499500851488637	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
56-57	0.1803981443420048	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
58-59	0.2146044982089377	1.1744670855599271E-4	0.0	0.0	2.3489341711198543E-4
60-61	0.25441893240941926	1.1744670855599271E-4	0.0	0.0	2.936167713899818E-4
62-63	0.30054612719478535	1.1744670855599271E-4	0.0	0.0	2.936167713899818E-4
64-65	0.3520465088965882	1.1744670855599271E-4	0.0	0.0	3.817018028069763E-4
66-67	0.4083915673263257	1.1744670855599271E-4	0.0	0.0	4.110634799459745E-4
68-69	0.47345704386634563	1.1744670855599271E-4	0.0	0.0	4.110634799459745E-4
70-71	0.547595278642316	1.1744670855599271E-4	0.0	0.0	4.404251570849727E-4
72-73	0.629719889600094	1.1744670855599271E-4	0.0	0.0	4.6978683422397086E-4
74-75	0.7230606612249691	1.7617006283398909E-4	0.0	0.0	4.6978683422397086E-4
76-77	0.8296729109166716	1.7617006283398909E-4	0.0	0.0	4.6978683422397086E-4
78-79	0.9461800458042163	1.7617006283398909E-4	0.0	0.0	4.6978683422397086E-4
80-81	1.0686769628281167	1.7617006283398909E-4	0.0	0.0	4.99148511362969E-4
82-83	1.207968759175524	1.7617006283398909E-4	0.0	0.0	5.872335427799636E-4
84-85	1.3605907569440365	1.7617006283398909E-4	0.0	0.0	5.872335427799636E-4
86-87	1.5277761465734923	1.7617006283398909E-4	0.0	0.0	5.872335427799636E-4
88-89	1.7068823771213812	1.7617006283398909E-4	0.0	0.0	6.753185741969582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	7910	0.0	91.12586	1
TCGACGG	8485	0.0	85.106064	2
TATCTCG	2260	0.0	83.664925	2
CGACGGC	8870	0.0	81.46563	3
ACGGCGC	9015	0.0	80.31341	5
GACGGCG	9080	0.0	79.68615	4
ATATCTC	2375	0.0	79.425415	1
CGGCGCG	9140	0.0	79.312	6
ATCTCGA	2515	0.0	75.370895	3
TCTCGAG	1950	0.0	72.84595	4
AGGGCGC	2030	0.0	70.42048	9
CTCGAGG	2045	0.0	69.46191	5
TCGAGGG	2070	0.0	68.84643	6
CGAGGGC	2065	0.0	68.77703	7
CGCGCCG	10945	0.0	66.60719	9
GCGCGCC	11260	0.0	64.79173	8
GGCGCGC	11265	0.0	64.68245	7
CTCGACG	775	0.0	61.913944	5
GAGGGCG	2325	0.0	61.8994	8
TCTCGAC	925	0.0	51.873848	4
>>END_MODULE