FastQCFastQC Report
Tue 31 May 2016
SRR2048257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431836
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT152950.4456798052121372No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT83550.24345568960754535No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.12888145004598123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG28250.072.8325651
TCGACGG34500.062.255182
ATATCTC25400.058.742461
TATCTCG25400.058.3683052
CGACGGC41550.051.8063933
ACGGCGC46450.046.220865
ATCTCGA54450.045.9905781
GACGGCG47100.045.600934
TCTCGAG52100.044.051992
CGGCGCG48850.043.9500276
CTCGAGG59050.038.62583
TCGAGGG59900.038.2363434
CGAGGGC61250.037.3788765
GGGCGCG63450.036.082848
AGGGCGC64300.035.531987
GAGGGCG68800.033.5531356
GGTCGAC4200.030.5471481
CGCGCCG108850.025.3095749
GCGCGCC114100.024.1866538
GGCGCGC115800.023.7085277
Produced by FastQC (version 0.11.5)