##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048257_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3431836
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30063470398935	33.0	31.0	34.0	30.0	34.0
2	32.43376052934931	34.0	31.0	34.0	31.0	34.0
3	32.512691748673305	34.0	31.0	34.0	31.0	34.0
4	36.06216992886606	37.0	35.0	37.0	35.0	37.0
5	35.92134152098177	37.0	35.0	37.0	35.0	37.0
6	35.909860494499156	37.0	35.0	37.0	35.0	37.0
7	35.885270741375756	37.0	35.0	37.0	35.0	37.0
8	35.854766078565525	37.0	35.0	37.0	35.0	37.0
9	37.45628928655099	39.0	37.0	39.0	34.0	39.0
10-11	37.45756236603381	39.0	37.0	39.0	34.0	39.0
12-13	37.46000639307939	39.0	37.0	39.0	34.0	39.0
14-15	38.80310408189669	40.0	38.0	41.0	35.0	41.0
16-17	38.81367437138604	40.0	38.0	41.0	35.0	41.0
18-19	38.85388316924235	40.0	38.0	41.0	35.0	41.0
20-21	38.88121780877641	40.0	38.0	41.0	35.0	41.0
22-23	38.86188631974255	40.0	38.0	41.0	35.0	41.0
24-25	38.84700521819808	40.0	38.0	41.0	35.0	41.0
26-27	38.75550492506052	40.0	38.0	41.0	34.5	41.0
28-29	38.66137965217452	40.0	38.0	41.0	34.0	41.0
30-31	38.531274513117765	40.0	38.0	41.0	34.0	41.0
32-33	38.44194390407933	40.0	38.0	41.0	34.0	41.0
34-35	38.39930623141665	40.0	38.0	41.0	34.0	41.0
36-37	38.317852309958866	40.0	38.0	41.0	34.0	41.0
38-39	38.226284414523306	40.0	38.0	41.0	33.5	41.0
40-41	38.0902340613013	40.0	37.0	41.0	33.0	41.0
42-43	38.019355674338755	40.0	37.0	41.0	33.0	41.0
44-45	37.908096715577315	40.0	37.0	41.0	33.0	41.0
46-47	37.797174457054474	40.0	37.0	41.0	33.0	41.0
48-49	37.69598372416397	40.0	37.0	41.0	33.0	41.0
50-51	37.604269259952986	40.0	36.0	41.0	32.5	41.0
52-53	37.578423765005084	40.0	36.0	41.0	32.5	41.0
54-55	37.40872830170206	39.0	36.0	41.0	32.0	41.0
56-57	37.17737648302541	39.0	35.5	41.0	32.0	41.0
58-59	36.93621169543067	39.0	35.0	41.0	31.5	41.0
60-61	36.67045278387428	38.5	35.0	40.5	31.0	41.0
62-63	36.36252446212465	38.0	35.0	40.0	31.0	41.0
64-65	36.04679521399041	37.0	35.0	40.0	31.0	41.0
66-67	35.707587134117134	37.0	35.0	40.0	30.5	41.0
68-69	35.348380720990164	36.0	34.0	39.0	30.0	41.0
70-71	34.9926163429721	36.0	34.0	39.0	30.0	41.0
72-73	34.620485652577806	35.5	34.0	38.5	29.5	40.0
74-75	34.28442195372972	35.0	34.0	37.0	29.0	39.5
76-77	34.03983203160058	35.0	34.0	37.0	29.5	39.0
78-79	33.88432751448496	35.0	34.0	36.5	30.0	39.0
80-81	33.642155394372	35.0	34.0	36.0	30.0	37.5
82-83	33.406989727947376	35.0	34.0	36.0	29.0	37.0
84-85	33.202763622737216	35.0	34.0	35.0	29.0	37.0
86-87	33.006811368608524	35.0	34.0	35.0	29.0	36.0
88-89	32.86790991760678	35.0	34.0	35.0	29.0	36.0
90-91	32.73482809201838	35.0	33.0	35.0	29.0	36.0
92-93	32.6019806890539	35.0	33.0	35.0	29.0	36.0
94-95	32.4910798476384	35.0	33.0	35.0	29.0	35.0
96-97	32.38200266562855	35.0	33.0	35.0	29.0	35.0
98-99	32.24338867008797	35.0	33.0	35.0	29.0	35.0
100-101	31.792766321001352	34.5	32.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	7.0
12	25.0
13	151.0
14	492.0
15	1103.0
16	1982.0
17	3033.0
18	5572.0
19	11530.0
20	13057.0
21	11985.0
22	11701.0
23	11910.0
24	13328.0
25	16048.0
26	20386.0
27	26862.0
28	35090.0
29	45304.0
30	59219.0
31	77644.0
32	100927.0
33	133633.0
34	181336.0
35	259361.0
36	426801.0
37	809000.0
38	937977.0
39	216038.0
40	334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.667893221004732	39.140535853111864	14.681703904265822	20.509867021617584
2	23.954262383167492	28.562670244149196	29.43806172556031	18.045005647123
3	22.535371736877867	27.575327026116632	25.00882909323173	24.880472143773773
4	23.508892321676207	26.962244619232205	26.03512967744399	23.493733381647598
5	23.46405830581648	27.602193111792054	25.472312779515104	23.461435802876363
6	23.73583702717729	26.29440917339873	26.078052680839058	23.891701118584923
7	24.142295843973898	27.573462135136996	24.86750532368097	23.41673669720814
8	23.987801281879438	26.018434447333732	26.4068854106082	23.586878860178633
9	23.131962016832972	25.725326035393298	26.776600047321608	24.36611190045212
10-11	23.993017148838113	26.434421691479432	25.83175594637972	23.740805213302735
12-13	23.807198246070033	26.563550822358646	25.686891797859808	23.94235913371152
14-15	23.467147031501508	26.48730883410513	26.016467570128643	24.029076564264727
16-17	23.655471881523475	26.78745429560154	25.88474507523087	23.672328747644116
18-19	23.120895963821102	26.68250367514755	26.572067130267047	23.6245332307643
20-21	22.62662026973317	26.757004122574624	26.896375584381072	23.720000023311137
22-23	22.483339450081292	26.841693560688896	27.40700345544052	23.267963533789292
24-25	22.438441114318984	26.361122734303155	28.306641109889867	22.893795041487998
26-27	22.388276938802985	26.18958259636101	28.80054804515603	22.621592419679974
28-29	22.39779581220637	26.423329120810436	28.685284910591456	22.493590156391736
30-31	22.477854780626867	26.280572014596853	28.62399727492766	22.61757592984862
32-33	22.401484919875227	26.26363155571291	28.725171810416295	22.609711713995573
34-35	22.460309874947406	26.469592369798555	28.56750730512763	22.502590450126405
36-37	22.480826951058695	26.412017708890765	28.424453911033904	22.682701429016632
38-39	22.442566051062794	26.372943209511767	28.56188973037299	22.622601009052453
40-41	22.63133471694787	26.660129828597405	28.031088347114842	22.677447107339884
42-43	22.676895417313794	26.698117330986676	27.848815355072947	22.776171896626582
44-45	22.608536400936995	26.663074793236596	27.97315186310708	22.755236942719332
46-47	22.684790123061077	26.934779013835904	27.69750563292352	22.682925230179503
48-49	22.761580501163298	26.933706466991207	27.488380489099782	22.816332542745712
50-51	22.668236480931377	26.788241312175128	27.734282176396636	22.80924003049686
52-53	22.701661247480722	27.04168686386122	27.53039705098377	22.726254837674293
54-55	22.78622466946296	26.890832701133792	27.435022101365714	22.887920528037533
56-57	22.715028403929505	26.87105995734348	27.58387176060363	22.830039878123387
58-59	22.842350937766852	27.04656292994063	27.313012295455163	22.798073836837354
60-61	22.802528338470065	26.94799754882178	27.275914026934405	22.97356008577375
62-63	22.812722913529367	26.92812918144987	27.347177477632883	22.911970427387885
64-65	22.91314631352553	27.134364794982503	27.117711867345374	22.8347770241466
66-67	22.901662218359043	27.063596836614405	27.02931483047162	23.005426114554933
68-69	22.852865650439817	27.06204083119343	27.058529567101928	23.02656395126482
70-71	22.96116713495526	27.215728668950685	26.874335721034527	22.948768475059534
72-73	22.955447271974958	27.16442308883082	26.744239411451463	23.13589022774276
74-75	22.89532434166214	27.109418059968725	26.95656848851137	23.03868910985777
76-77	22.970226807295973	27.289461077704726	26.742301151694793	22.998010963304516
78-79	22.998624496205892	27.1903342630113	26.721386687320347	23.08965455346246
80-81	22.884634184826165	27.212813667787984	26.835974509850484	23.066577637535364
82-83	22.984510100888684	27.41291803531869	26.655957852438366	22.946614011354256
84-85	22.97196019856427	27.25670748835317	26.6715105267268	23.099821786355758
86-87	22.935047867977335	27.220044087201163	26.794673986365897	23.050234058455608
88-89	22.974488202192187	27.37673901691928	26.633140277589995	23.015632503298537
90-91	23.021972546409668	27.323382756944696	26.53649891544681	23.118145781198823
92-93	22.90696641000051	27.282932550465134	26.724425050811156	23.0856759887232
94-95	23.019748029917515	27.435460202643714	26.509804081547024	23.03498768589175
96-97	22.962669953552343	27.41983845306572	26.488685304007788	23.12880628937415
98-99	22.94541464102597	27.31230455068366	26.658820526388787	23.083460281901583
100-101	23.01109113779929	27.47901915869147	26.384141038229814	23.12574866527943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1486.0
1	1166.5
2	601.5
3	299.5
4	206.0
5	146.5
6	119.0
7	112.5
8	114.5
9	119.0
10	124.0
11	127.5
12	153.0
13	223.5
14	306.5
15	448.5
16	1927.5
17	2345.5
18	1490.5
19	1783.0
20	2368.0
21	3155.5
22	4272.5
23	5811.5
24	7420.0
25	9576.0
26	12547.0
27	16869.5
28	22030.0
29	27892.0
30	35153.0
31	42213.0
32	49216.5
33	59573.0
34	70599.0
35	81038.5
36	91144.0
37	98421.0
38	103501.0
39	109379.0
40	113713.0
41	113971.5
42	113932.5
43	116624.5
44	120072.0
45	123987.0
46	127320.0
47	130057.0
48	132501.0
49	130571.0
50	125640.0
51	121279.5
52	120504.5
53	119387.0
54	113493.5
55	106084.5
56	98019.5
57	88275.0
58	80012.5
59	73008.5
60	64043.5
61	54413.0
62	44917.0
63	34711.5
64	25926.0
65	18970.5
66	13635.5
67	9957.0
68	7372.0
69	5631.5
70	4402.0
71	3571.5
72	3067.5
73	2376.0
74	1712.0
75	1334.5
76	1034.5
77	742.5
78	481.0
79	230.5
80	60.5
81	23.0
82	19.0
83	8.5
84	1.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.044203744001753
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.9138921556857614E-5
20-21	0.0
22-23	4.370838233528642E-5
24-25	0.0
26-27	5.827784311371523E-5
28-29	1.4569460778428804E-4
30-31	1.4569460778428807E-5
32-33	2.9138921556857614E-5
34-35	0.0
36-37	1.4569460778428807E-5
38-39	7.284730389214402E-5
40-41	1.0198622544900164E-4
42-43	1.7483352934114568E-4
44-45	4.3708382335286413E-4
46-47	1.0198622544900164E-4
48-49	1.4569460778428807E-5
50-51	2.1854191167643207E-4
52-53	0.0013840987739507367
54-55	0.0010052927937115876
56-57	1.3112514700585926E-4
58-59	0.0011509874014958756
60-61	4.953616664665793E-4
62-63	3.4966705868229135E-4
64-65	2.0397245089800328E-4
66-67	1.894029901195745E-4
68-69	6.847646565861539E-4
70-71	3.9337544101757777E-4
72-73	1.0198622544900164E-4
74-75	8.887371074841571E-4
76-77	6.993341173645827E-4
78-79	7.284730389214402E-5
80-81	1.4569460778428807E-5
82-83	0.0024330999499976107
84-85	0.0
86-87	2.9138921556857614E-5
88-89	3.4966705868229135E-4
90-91	2.331113724548609E-4
92-93	3.205281371254337E-4
94-95	0.0
96-97	4.662227449097218E-4
98-99	0.0
100-101	0.009105912986518003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3431836.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.335994772823376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.03453705378682	49.86272851360127
2	8.221226558764801	9.756293122341301
3	2.498159979024873	4.446924223712892
4	1.2442969173093823	2.9532638152523893
5	0.7552345110881125	2.240629550109003
6	0.5426910488187764	1.9320667941581369
7	0.4337971787331565	1.8017850990843267
8	0.3403543405711032	1.6156210698427782
9	0.25731091614719265	1.3741019257950196
>10	1.5446301411477856	17.640895985116423
>50	0.11898570635560969	4.590008548462098
>100	0.008283768289422667	0.6353077954490416
>500	4.9187996296948135E-5	0.021014054576361865
>1k	3.4431597406460663E-4	0.4391061820673352
>5k	4.9187996294943804E-5	0.24385059163659595
>10k+	4.9187996294943804E-5	0.4464027287949413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15295	0.4456798052121372	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	8355	0.24345568960754535	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4423	0.12888145004598123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9138921556857614E-5	0.0	0.0	0.0	0.0
2	2.9138921556857614E-5	0.0	0.0	0.0	0.0
3	2.9138921556857614E-5	0.0	0.0	0.0	0.0
4	1.1655568622743045E-4	0.0	0.0	0.0	2.9138921556857614E-5
5	1.7483352934114568E-4	0.0	0.0	0.0	2.9138921556857614E-5
6	3.205281371254337E-4	0.0	0.0	0.0	2.9138921556857614E-5
7	5.24500588023437E-4	0.0	0.0	0.0	2.9138921556857614E-5
8	0.0013986682347291654	0.0	0.0	0.0	2.9138921556857614E-5
9	0.0038754765670620623	0.0	0.0	0.0	2.9138921556857614E-5
10-11	0.013025097935915352	0.0	0.0	0.0	2.9138921556857614E-5
12-13	0.02318001209848023	0.0	0.0	0.0	5.827784311371523E-5
14-15	0.0330289675846981	0.0	0.0	0.0	5.827784311371523E-5
16-17	0.04254282547301211	0.0	0.0	0.0	8.741676467057284E-5
18-19	0.04955073610743637	0.0	0.0	0.0	8.741676467057284E-5
20-21	0.058904329927187665	0.0	0.0	0.0	8.741676467057284E-5
22-23	0.06873871595262711	0.0	0.0	0.0	8.741676467057284E-5
24-25	0.07946183908555071	0.0	0.0	0.0	8.741676467057284E-5
26-27	0.09464321721667353	0.0	0.0	0.0	8.741676467057284E-5
28-29	0.10832394088761817	0.0	0.0	0.0	8.741676467057284E-5
30-31	0.1201252041181455	1.4569460778428807E-5	0.0	0.0	1.1655568622743045E-4
32-33	0.13338341342651572	2.9138921556857614E-5	0.0	0.0	1.7483352934114568E-4
34-35	0.14702042871512508	2.9138921556857614E-5	0.0	2.9138921556857614E-5	1.7483352934114568E-4
36-37	0.1612402224348716	4.370838233528642E-5	0.0	2.9138921556857614E-5	2.0397245089800328E-4
38-39	0.175795113752522	5.827784311371523E-5	0.0	2.9138921556857614E-5	2.3311137245486088E-4
40-41	0.19266654933394253	5.827784311371523E-5	0.0	2.9138921556857614E-5	3.0595867634700493E-4
42-43	0.21189823756146856	5.827784311371523E-5	0.0	2.9138921556857614E-5	3.205281371254337E-4
44-45	0.23451004068959005	5.827784311371523E-5	0.0	2.9138921556857614E-5	3.205281371254337E-4
46-47	0.2594675270030386	5.827784311371523E-5	0.0	2.9138921556857614E-5	3.205281371254337E-4
48-49	0.2866832797371436	5.827784311371523E-5	0.0	4.370838233528642E-5	3.205281371254337E-4
50-51	0.3178764952637597	5.827784311371523E-5	0.0	5.827784311371523E-5	3.205281371254337E-4
52-53	0.35287234005354573	5.827784311371523E-5	0.0	5.827784311371523E-5	3.4966705868229135E-4
54-55	0.38944168660740197	5.827784311371523E-5	0.0	5.827784311371523E-5	3.642365194607202E-4
56-57	0.43393681982472354	5.827784311371523E-5	0.0	5.827784311371523E-5	4.0794490179600656E-4
58-59	0.48264252720701106	5.827784311371523E-5	0.0	5.827784311371523E-5	4.0794490179600656E-4
60-61	0.5363455596362996	5.827784311371523E-5	0.0	5.827784311371523E-5	4.2251436257443535E-4
62-63	0.5965174326512106	5.827784311371523E-5	0.0	5.827784311371523E-5	4.370838233528642E-4
64-65	0.6629687432616244	5.827784311371523E-5	0.0	1.0198622544900165E-4	4.662227449097218E-4
66-67	0.7346067819091588	7.284730389214404E-5	0.0	1.3112514700585926E-4	4.662227449097218E-4
68-69	0.8143891491318349	1.1655568622743045E-4	0.0	1.4569460778428807E-4	5.099311272450083E-4
70-71	0.9026800814491136	1.1655568622743045E-4	0.0	1.4569460778428807E-4	5.24500588023437E-4
72-73	1.000106065674467	1.1655568622743045E-4	0.0	1.4569460778428807E-4	5.24500588023437E-4
74-75	1.1098432442575927	1.1655568622743045E-4	0.0	1.602640685627169E-4	5.390700488018658E-4
76-77	1.2279287238667582	1.1655568622743045E-4	0.0	1.7483352934114568E-4	5.536395095802946E-4
78-79	1.3562565344031592	1.1655568622743045E-4	0.0	1.7483352934114568E-4	5.536395095802946E-4
80-81	1.4927141040539234	1.1655568622743045E-4	0.0	1.7483352934114568E-4	5.827784311371523E-4
82-83	1.6435371620322183	1.1655568622743045E-4	0.0	1.7483352934114568E-4	6.264868134724386E-4
84-85	1.8064674419173876	1.1655568622743045E-4	0.0	1.7483352934114568E-4	6.410562742508674E-4
86-87	1.9814758047878747	1.3112514700585926E-4	0.0	1.8940299011957446E-4	6.410562742508674E-4
88-89	2.1700337661823	1.4569460778428807E-4	0.0	2.0397245089800328E-4	6.556257350292963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	2825	0.0	72.832565	1
TCGACGG	3450	0.0	62.25518	2
ATATCTC	2540	0.0	58.74246	1
TATCTCG	2540	0.0	58.368305	2
CGACGGC	4155	0.0	51.806393	3
ACGGCGC	4645	0.0	46.22086	5
ATCTCGA	5445	0.0	45.990578	1
GACGGCG	4710	0.0	45.60093	4
TCTCGAG	5210	0.0	44.05199	2
CGGCGCG	4885	0.0	43.950027	6
CTCGAGG	5905	0.0	38.6258	3
TCGAGGG	5990	0.0	38.236343	4
CGAGGGC	6125	0.0	37.378876	5
GGGCGCG	6345	0.0	36.08284	8
AGGGCGC	6430	0.0	35.53198	7
GAGGGCG	6880	0.0	33.553135	6
GGTCGAC	420	0.0	30.547148	1
CGCGCCG	10885	0.0	25.309574	9
GCGCGCC	11410	0.0	24.186653	8
GGCGCGC	11580	0.0	23.708527	7
>>END_MODULE