FastQCFastQC Report
Tue 31 May 2016
SRR2048256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14141595
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT920990.6512631708092333No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG396300.2802371302529877No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG281020.1987187442434888No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTGGG252770.17874221401475576No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA223810.15826361877850414No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA219670.1553360847910013No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC212300.15012450858619553No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC187880.13285630086280933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC796550.092.1024861
TATCTCG827750.088.552152
GTCGACG601000.087.9042361
CTCGACG263300.081.6507345
ATCTCGA909950.080.49453
TCTCGAG663150.077.791584
TCTCGAC281200.076.720844
CTCGAGG690150.074.654315
TCGAGGG697750.073.742026
CGAGGGC701700.073.326917
AGGGCGC718800.071.5956959
GAGGGCG725900.070.869278
TCGACGG840100.062.864122
CGACGGC868900.060.7727743
CGGCGCG897850.058.639576
GACGGCG909850.057.9405564
ACGGCGC909700.057.915435
GGTCGAC12800.036.4760671
GGGCGCG719250.035.81117610-11
GATCCTT704500.035.47227520-21
Produced by FastQC (version 0.11.5)