##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048256_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14141595 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.279108191119885 31.0 31.0 34.0 27.0 34.0 2 30.681216439871175 31.0 31.0 34.0 27.0 34.0 3 30.81630848571183 31.0 31.0 34.0 27.0 34.0 4 34.26043314067473 37.0 35.0 37.0 30.0 37.0 5 34.26632328248688 37.0 35.0 37.0 30.0 37.0 6 34.17932687225168 37.0 35.0 37.0 30.0 37.0 7 33.95787434161422 37.0 35.0 37.0 29.0 37.0 8 34.05272410926773 37.0 35.0 37.0 30.0 37.0 9 35.41919910731428 39.0 35.0 39.0 29.0 39.0 10-11 35.36960081235533 39.0 35.0 39.0 29.0 39.0 12-13 35.15049837023334 39.0 35.0 39.0 27.0 39.0 14-15 36.44677683104346 40.0 36.0 41.0 28.0 41.0 16-17 36.44691415643002 40.0 36.5 41.0 28.0 41.0 18-19 36.37947201146688 40.0 36.0 41.0 27.0 41.0 20-21 36.54374884869776 40.0 36.5 41.0 29.0 41.0 22-23 36.664128940193805 40.0 37.0 41.0 30.0 41.0 24-25 36.56042971107573 40.0 36.5 41.0 29.0 41.0 26-27 36.64950258439731 40.0 37.0 41.0 30.0 41.0 28-29 36.63845047181736 40.0 36.5 41.0 30.0 41.0 30-31 36.287442894524986 39.5 35.5 41.0 30.0 41.0 32-33 35.89649859156623 38.5 35.0 40.5 29.5 41.0 34-35 35.66944347508184 39.0 35.0 41.0 29.0 41.0 36-37 35.444957340384875 38.5 35.0 40.0 28.0 41.0 38-39 35.25085869026796 38.5 35.0 40.0 27.0 41.0 40-41 34.83558792342731 38.5 34.5 40.5 23.5 41.0 42-43 34.56440309597326 38.5 34.0 40.5 22.5 41.0 44-45 34.053578645124546 38.0 33.0 40.0 18.5 41.0 46-47 33.51028246813743 38.0 33.0 40.0 14.0 41.0 48-49 32.817067204936926 38.0 32.0 40.0 6.0 41.0 50-51 32.709406435412696 38.0 32.0 40.0 2.0 41.0 52-53 32.621893393213426 38.0 32.0 40.0 2.0 41.0 54-55 32.56109289652264 38.0 32.0 40.0 2.0 41.0 56-57 32.346902064441814 38.0 32.0 40.0 2.0 41.0 58-59 32.108708671122315 37.0 31.0 40.0 2.0 41.0 60-61 31.85156614229159 37.0 31.0 40.0 2.0 41.0 62-63 31.57294619171317 36.0 31.0 40.0 2.0 41.0 64-65 31.30999151792991 36.0 31.0 39.5 2.0 41.0 66-67 31.011465078726978 35.5 30.0 39.0 2.0 41.0 68-69 30.692711253575006 35.0 30.0 39.0 2.0 41.0 70-71 30.383312455207495 35.0 30.0 38.5 2.0 40.0 72-73 30.078216778234705 35.0 29.5 37.5 2.0 40.0 74-75 29.788641062058417 35.0 29.0 37.0 2.0 39.5 76-77 29.50882746960297 35.0 29.0 36.5 2.0 39.0 78-79 29.235760251937634 35.0 29.0 36.0 2.0 39.0 80-81 28.983707743009187 34.0 29.0 36.0 2.0 37.5 82-83 28.73967367188779 34.0 29.0 35.0 2.0 37.0 84-85 28.581777691978875 34.0 29.0 35.0 2.0 37.0 86-87 28.628613816192583 34.0 29.0 35.0 2.0 36.0 88-89 28.517166840091235 34.0 29.0 35.0 2.0 36.0 90-91 28.370004196839183 34.0 29.0 35.0 2.0 36.0 92-93 28.23780743968414 34.0 29.0 35.0 2.0 36.0 94-95 28.11047459639454 34.0 29.0 35.0 2.0 35.0 96-97 27.94551141508437 34.0 29.0 35.0 2.0 35.0 98-99 27.772438469635144 34.0 29.0 35.0 2.0 35.0 100-101 27.194817380924853 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7868666421620887 1101 2 0.765139819796623 1101 3 0.7679572404313788 1101 4 0.8299145442859057 1101 5 0.855560057866839 1101 6 0.816977077382937 1101 7 0.875866430731989 1101 8 0.8432465595122167 1101 9 0.9689721997886593 1101 10-11 0.9161198884010702 1101 12-13 0.902579353188969 1101 14-15 1.0779923462278305 1101 16-17 1.1129173999491755 1101 18-19 1.0527743162958743 1101 20-21 1.074322381127999 1101 22-23 1.0912197415859595 1101 24-25 1.1094275453205924 1101 26-27 1.0704149645337964 1101 28-29 1.0522517952497168 1101 30-31 0.9796702471537913 1101 32-33 0.9277101405792436 1101 34-35 1.0237986720329317 1101 36-37 0.9548190538588273 1101 38-39 0.8935728385125827 1101 40-41 0.8428319713023882 1101 42-43 0.8392023522706822 1101 44-45 0.7571907984712283 1101 46-47 0.6768357010820054 1101 48-49 0.4412118667478637 1101 50-51 0.33558295683938155 1101 52-53 0.2695234761443501 1101 54-55 0.20210136688685765 1101 56-57 0.16705573318485278 1101 58-59 0.16839258735090823 1101 60-61 0.1844940466282594 1101 62-63 0.1619813806361421 1101 64-65 0.15634343106687965 1101 66-67 0.13982838053138735 1101 68-69 0.09131662656114514 1101 70-71 0.11701210895569858 1101 72-73 0.1049563296770657 1101 74-75 0.0842079418934496 1101 76-77 0.10041926031712123 1101 78-79 0.07133868035043278 1101 80-81 0.03206776235770903 1101 82-83 0.00827102115165701 1101 84-85 0.0456849640703858 1101 86-87 0.08357071551111162 1101 88-89 0.13523643693571685 1101 90-91 0.10972556451639193 1101 92-93 0.059483477400263496 1101 94-95 0.03254388276945264 1101 96-97 0.0328257836590069 1101 98-99 0.056109041100658175 1101 100-101 0.03379016683581426 1102 1 0.6333304197802647 1102 2 0.6430147159243269 1102 3 0.662564489407913 1102 4 0.7208557659350348 1102 5 0.755043541635402 1102 6 0.7311211289819681 1102 7 0.7807201153958303 1102 8 0.7517909435617511 1102 9 0.8613338404581086 1102 10-11 0.8571923135966273 1102 12-13 0.8229638582590013 1102 14-15 0.9819915116512874 1102 16-17 1.0103331835996485 1102 18-19 1.0276472949972728 1102 20-21 0.9915999751283948 1102 22-23 0.9848287247289633 1102 24-25 0.975167962391005 1102 26-27 0.9693353334016521 1102 28-29 0.916689990474481 1102 30-31 0.8856248352373797 1102 32-33 0.7893826944312892 1102 34-35 0.8848277129998863 1102 36-37 0.8639558864594505 1102 38-39 0.7944972471311615 1102 40-41 0.7153995271735951 1102 42-43 0.7478600599562526 1102 44-45 0.6724868424689703 1102 46-47 0.5829674062517256 1102 48-49 0.3881316264418686 1102 50-51 0.2862707751812721 1102 52-53 0.22334554912662696 1102 54-55 0.1643030145199802 1102 56-57 0.18471796583090594 1102 58-59 0.21412142302050086 1102 60-61 0.2551766335868564 1102 62-63 0.20989332255805238 1102 64-65 0.23579024487670708 1102 66-67 0.18494651560078879 1102 68-69 0.17909536401323223 1102 70-71 0.11935282704937578 1102 72-73 0.18360325593686255 1102 74-75 0.14006294466772928 1102 76-77 0.14238140636575025 1102 78-79 0.1691514596239152 1102 80-81 0.15301775317253785 1102 82-83 0.15096349438218937 1102 84-85 0.08957573386177486 1102 86-87 0.15319284387551235 1102 88-89 0.17733386403755702 1102 90-91 0.22220168048033528 1102 92-93 0.1988799339396614 1102 94-95 0.21531645233018892 1102 96-97 0.2104430304012297 1102 98-99 0.24127618659403538 1102 100-101 0.22815168595637658 1103 1 0.5623336833474717 1103 2 0.5444426392410904 1103 3 0.5531909344338217 1103 4 0.588645548207694 1103 5 0.6263585017068607 1103 6 0.5948652610471683 1103 7 0.6139161183270403 1103 8 0.6146205651943575 1103 9 0.7072694183332757 1103 10-11 0.6265282815577109 1103 12-13 0.5939199340014767 1103 14-15 0.7495927253420973 1103 16-17 0.7753649533474061 1103 18-19 0.7736234981707568 1103 20-21 0.7715403587182337 1103 22-23 0.7562374665249152 1103 24-25 0.7692283293003541 1103 26-27 0.7759793935455832 1103 28-29 0.7404873749961638 1103 30-31 0.6831785884906765 1103 32-33 0.6088217003048939 1103 34-35 0.6246263060876203 1103 36-37 0.5869055240194001 1103 38-39 0.5834135046544944 1103 40-41 0.45770679849796636 1103 42-43 0.511276045645424 1103 44-45 0.3929344684655547 1103 46-47 0.2920587406901376 1103 48-49 0.14527047265980286 1103 50-51 0.11966108019850452 1103 52-53 0.060467479383710554 1103 54-55 -0.020149642756287278 1103 56-57 0.006803016915007731 1103 58-59 -0.02889558651678925 1103 60-61 -0.036055834572501055 1103 62-63 -0.04928202069362797 1103 64-65 -0.04463175542351294 1103 66-67 -0.007766371650820503 1103 68-69 -0.037535964920074605 1103 70-71 -0.05155228657098121 1103 72-73 -0.05055434766413569 1103 74-75 -0.1141542092783645 1103 76-77 -0.06593849806263208 1103 78-79 -0.07155799970003685 1103 80-81 -0.05620048046553805 1103 82-83 -0.08625938565936053 1103 84-85 -0.09530347135090977 1103 86-87 -0.07810653003055634 1103 88-89 -0.056408152529769495 1103 90-91 -0.025232868974061518 1103 92-93 -0.038287082187622445 1103 94-95 -0.01671950462162286 1103 96-97 0.02655349643413274 1103 98-99 0.03699283544909093 1103 100-101 0.018500770948094214 1104 1 0.5211644321325437 1104 2 0.48247097409166173 1104 3 0.5244019173887295 1104 4 0.5248207931617443 1104 5 0.5633159961983836 1104 6 0.5047066511773295 1104 7 0.5075707144825898 1104 8 0.575730157123381 1104 9 0.6934851606978896 1104 10-11 0.5738526821368879 1104 12-13 0.5958598981524048 1104 14-15 0.6901228442150966 1104 16-17 0.7809163423955923 1104 18-19 0.7067057089620548 1104 20-21 0.7487477826366984 1104 22-23 0.7206699518855615 1104 24-25 0.6944947747663335 1104 26-27 0.7828977885043997 1104 28-29 0.7202537267185818 1104 30-31 0.6452945453982153 1104 32-33 0.6139006503826678 1104 34-35 0.6669015660509601 1104 36-37 0.6189948529885925 1104 38-39 0.5949919646097896 1104 40-41 0.5766430907129205 1104 42-43 0.6009221912422262 1104 44-45 0.5405888154266449 1104 46-47 0.48458209337960767 1104 48-49 0.38068421708367595 1104 50-51 0.341730761989119 1104 52-53 0.25003616457682654 1104 54-55 0.19045922938389737 1104 56-57 0.23811246419180065 1104 58-59 0.28002580356971407 1104 60-61 0.23832983302103372 1104 62-63 0.23389459593490614 1104 64-65 0.2487260562807272 1104 66-67 0.2640761454019227 1104 68-69 0.25762822470410285 1104 70-71 0.2788110319528485 1104 72-73 0.25913131396138667 1104 74-75 0.2466035033389069 1104 76-77 0.23293221949993992 1104 78-79 0.25336900266329465 1104 80-81 0.2735367870787684 1104 82-83 0.261623317724073 1104 84-85 0.2254777509180279 1104 86-87 0.24742644959286864 1104 88-89 0.27692599428421616 1104 90-91 0.2234620915662724 1104 92-93 0.24262374047020074 1104 94-95 0.2469858267468581 1104 96-97 0.2531135103694453 1104 98-99 0.27686506071167827 1104 100-101 0.25382955192195666 1105 1 0.3274180662142321 1105 2 0.3338260646732074 1105 3 0.34154073356499026 1105 4 0.3553783675399913 1105 5 0.416380739834608 1105 6 0.316200700455326 1105 7 0.40196567756257906 1105 8 0.36699248175843735 1105 9 0.44691680766616315 1105 10-11 0.3238383975368748 1105 12-13 0.296546191840136 1105 14-15 0.37244455397383547 1105 16-17 0.43378799376712607 1105 18-19 0.43434955566518596 1105 20-21 0.4118727797467514 1105 22-23 0.40031679274439824 1105 24-25 0.41155375741288225 1105 26-27 0.42849229869406 1105 28-29 0.3780139404463796 1105 30-31 0.3566341804040718 1105 32-33 0.31140146041611416 1105 34-35 0.32717440766865735 1105 36-37 0.30786876884747727 1105 38-39 0.3548848122121555 1105 40-41 0.26815371923193254 1105 42-43 0.2630504441788375 1105 44-45 0.23412977857136497 1105 46-47 0.16941535538739316 1105 48-49 0.08498360407192962 1105 50-51 -0.001191775050820354 1105 52-53 -0.018008889297071562 1105 54-55 -0.05836924149628686 1105 56-57 -0.08494185456520142 1105 58-59 -0.1306843262304085 1105 60-61 -0.10247519561877283 1105 62-63 -0.07308986916870808 1105 64-65 -0.09486261042072996 1105 66-67 -0.13658546567529584 1105 68-69 -0.14277196993601748 1105 70-71 -0.11903553993685634 1105 72-73 -0.12005421889565682 1105 74-75 -0.10520825927691746 1105 76-77 -0.12131675786909213 1105 78-79 -0.09626871122655345 1105 80-81 -0.0985627852553499 1105 82-83 -0.07162563731090899 1105 84-85 -0.11868453586696859 1105 86-87 -0.10226432329553248 1105 88-89 -0.11646800909202781 1105 90-91 -0.09552308224630934 1105 92-93 -0.20121945544252995 1105 94-95 -0.13698499355329474 1105 96-97 -0.15772042301676237 1105 98-99 -0.1253358080705098 1105 100-101 -0.1468034029220675 1106 1 0.2909540101357422 1106 2 0.24549647072618797 1106 3 0.30511393011699894 1106 4 0.27536740161509243 1106 5 0.31882282278079543 1106 6 0.2352246696279181 1106 7 0.23167045913833562 1106 8 0.29356997619569825 1106 9 0.3308690734208497 1106 10-11 0.2470227809545804 1106 12-13 0.2950739880754796 1106 14-15 0.3308423768427815 1106 16-17 0.3702414220161714 1106 18-19 0.3915889270030064 1106 20-21 0.428760377868862 1106 22-23 0.40809638158891914 1106 24-25 0.35320429651807217 1106 26-27 0.35305528831870703 1106 28-29 0.3880495872529153 1106 30-31 0.3350055529561473 1106 32-33 0.30292913401203947 1106 34-35 0.2985155974612397 1106 36-37 0.3384702536853794 1106 38-39 0.31777391343518957 1106 40-41 0.29214478648778197 1106 42-43 0.28233428733933863 1106 44-45 0.2496445240634131 1106 46-47 0.2299231411008691 1106 48-49 0.09678296489030203 1106 50-51 0.09693471491417682 1106 52-53 0.008471617135739962 1106 54-55 0.02052711830298648 1106 56-57 0.030064894313596824 1106 58-59 0.05494840723920902 1106 60-61 0.01691474548410099 1106 62-63 0.0026935898073254805 1106 64-65 -0.02801538182745844 1106 66-67 0.017948606605713735 1106 68-69 -0.015488813856574524 1106 70-71 0.0070346900821292024 1106 72-73 0.0472924607282863 1106 74-75 -0.013637951827345063 1106 76-77 0.02440752249456679 1106 78-79 -0.03290685582844333 1106 80-81 -0.04731154257335035 1106 82-83 0.004385276717457032 1106 84-85 -0.03237876204751089 1106 86-87 -0.01773579852403273 1106 88-89 -7.944342441739138E-4 1106 90-91 0.0024097427955744877 1106 92-93 -0.006142655913095041 1106 94-95 -0.035349423585373074 1106 96-97 -0.02018741319078643 1106 98-99 -0.07453401315472163 1106 100-101 -0.04111386958923191 1107 1 0.16527096197217261 1107 2 0.13846314976917284 1107 3 0.18076492687529466 1107 4 0.20077387988112605 1107 5 0.1773183932257325 1107 6 0.12102392899790004 1107 7 0.09091952405370307 1107 8 0.12659781167059947 1107 9 0.20967753926606747 1107 10-11 0.1074069154906141 1107 12-13 0.12608213873127738 1107 14-15 0.10511449490468294 1107 16-17 0.11684676894302726 1107 18-19 0.18083906798636207 1107 20-21 0.19052150171530968 1107 22-23 0.1906340853580062 1107 24-25 0.16271951058869405 1107 26-27 0.15485587505302334 1107 28-29 0.1878265833679933 1107 30-31 0.14347657003482084 1107 32-33 0.05068235782803043 1107 34-35 0.11013535307682076 1107 36-37 0.06851634900591819 1107 38-39 0.052215458228054956 1107 40-41 -0.04019918157617042 1107 42-43 -0.02867430867473786 1107 44-45 -0.05171360578192008 1107 46-47 -0.06642272565039065 1107 48-49 0.019556675377096155 1107 50-51 -0.046585209851045306 1107 52-53 -0.11241832636748228 1107 54-55 -0.11193407567356672 1107 56-57 -0.17986370006354235 1107 58-59 -0.1652129604186321 1107 60-61 -0.08138863001050467 1107 62-63 -0.06093279225667558 1107 64-65 -0.08002749264385756 1107 66-67 -0.10560853416779281 1107 68-69 -0.15597216892529886 1107 70-71 -0.11926912498063302 1107 72-73 -0.10528884544378059 1107 74-75 -0.08665596853006363 1107 76-77 -0.1280210723784485 1107 78-79 -0.09627239121431685 1107 80-81 -0.07924000904450779 1107 82-83 -0.06043896423881989 1107 84-85 -0.1288590045712361 1107 86-87 -0.07800391623631597 1107 88-89 -0.08960775041370539 1107 90-91 -0.06941626527466482 1107 92-93 -0.11118656463969145 1107 94-95 -0.08591293938855671 1107 96-97 -0.10298280397018544 1107 98-99 -0.11331856208285274 1107 100-101 -0.14757473761400064 1108 1 0.13204685623244572 1108 2 0.08833203750131347 1108 3 0.09148845033742603 1108 4 0.10616973930975604 1108 5 0.07385900141440516 1108 6 0.06594710387783209 1108 7 0.0318395921188781 1108 8 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-1.012701062946654 2215 22-23 -1.0628902589278582 2215 24-25 -0.9328535056725542 2215 26-27 -1.0953540650239901 2215 28-29 -0.9808687252420114 2215 30-31 -0.9219827495178379 2215 32-33 -1.0174259027561092 2215 34-35 -0.9182306143839369 2215 36-37 -0.8506520174480485 2215 38-39 -0.8765099365621865 2215 40-41 -0.9603567530236532 2215 42-43 -0.890355057899491 2215 44-45 -0.7128856063882694 2215 46-47 -0.777156546587527 2215 48-49 -0.596785061901322 2215 50-51 -0.44584276923531974 2215 52-53 -0.42025743098077584 2215 54-55 -0.37483151093010747 2215 56-57 -0.3981011726055428 2215 58-59 -0.42280080528959374 2215 60-61 -0.3186981240511244 2215 62-63 -0.41428707411317234 2215 64-65 -0.36564026755639745 2215 66-67 -0.4223219006844623 2215 68-69 -0.41566832494428496 2215 70-71 -0.37332751704474276 2215 72-73 -0.5114687009093224 2215 74-75 -0.49375193041090526 2215 76-77 -0.43818185127499376 2215 78-79 -0.351924351032082 2215 80-81 -0.3585689501689693 2215 82-83 -0.3156225079312023 2215 84-85 -0.32626616400580843 2215 86-87 -0.4673362109725616 2215 88-89 -0.6136719452717259 2215 90-91 -0.5162886370300548 2215 92-93 -0.44174455297233095 2215 94-95 -0.4720002887745949 2215 96-97 -0.5886148708312859 2215 98-99 -0.591520445150362 2215 100-101 -0.6095087434048949 2216 1 -0.7117657570636986 2216 2 -0.8542445327729773 2216 3 -0.8386446077082645 2216 4 -0.7106421887930097 2216 5 -1.0144890355299125 2216 6 -0.6723135429261546 2216 7 -0.7573510480071945 2216 8 -0.6663201389298621 2216 9 -0.9158867003686311 2216 10-11 -0.7643950157340669 2216 12-13 -0.7881233606821993 2216 14-15 -0.9578243295473001 2216 16-17 -1.0947484254089517 2216 18-19 -0.8413397485472984 2216 20-21 -1.0395157176576504 2216 22-23 -1.1757660216511852 2216 24-25 -1.03599465103931 2216 26-27 -1.5292473626368022 2216 28-29 -1.3336246706808836 2216 30-31 -1.1036810167343987 2216 32-33 -1.1111688952795973 2216 34-35 -1.156291388572761 2216 36-37 -1.2251475847789806 2216 38-39 -1.1686907195966256 2216 40-41 -1.1681557222555483 2216 42-43 -1.1578312465647045 2216 44-45 -0.9223066831873936 2216 46-47 -1.5499503183142274 2216 48-49 -1.3241121779479528 2216 50-51 -1.1774873711543066 2216 52-53 -1.297701702175054 2216 54-55 -1.1390131292275782 2216 56-57 -1.232538518018501 2216 58-59 -1.1361047987765147 2216 60-61 -1.0843705037478948 2216 62-63 -0.9978411605635991 2216 64-65 -0.9449524361978341 2216 66-67 -1.181365665368471 2216 68-69 -1.019972253638457 2216 70-71 -1.241346521039354 2216 72-73 -1.1438861676255705 2216 74-75 -0.8593807510702156 2216 76-77 -1.0099762652950197 2216 78-79 -1.0094604870743176 2216 80-81 -0.9392324701617589 2216 82-83 -1.0520192705474756 2216 84-85 -0.9336192957491853 2216 86-87 -0.8707816709728995 2216 88-89 -0.861287994717042 2216 90-91 -0.7751215787898253 2216 92-93 -0.8989526757292339 2216 94-95 -1.0410021705813541 2216 96-97 -0.9909782215178069 2216 98-99 -0.9732539962436704 2216 100-101 -1.1702861366518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 313046.0 3 56705.0 4 31438.0 5 23310.0 6 21496.0 7 26700.0 8 33983.0 9 38413.0 10 42725.0 11 45460.0 12 51418.0 13 67373.0 14 89704.0 15 135465.0 16 135649.0 17 227102.0 18 343852.0 19 132613.0 20 86135.0 21 72917.0 22 76434.0 23 88743.0 24 108517.0 25 133260.0 26 162039.0 27 194057.0 28 234956.0 29 282149.0 30 321248.0 31 361073.0 32 441496.0 33 550298.0 34 695207.0 35 938797.0 36 1537538.0 37 2587336.0 38 2807765.0 39 644889.0 40 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.70851137403534 43.262084823680695 11.862195785717576 20.167208016566388 2 21.433201003184067 26.515967228376585 37.474970840759575 14.575860927679773 3 18.712856367142034 31.77031358920548 24.1261783778134 25.390651665839087 4 23.2597740897849 26.154265536903175 29.84752866006623 20.738431713245692 5 20.967781905845687 29.612823230266322 24.235805510413662 25.18358935347433 6 20.48925800810856 25.165682013873102 30.29058261457727 24.05447736344107 7 24.52011500458652 26.453115380217184 23.511137750244902 25.515631864951395 8 29.150921700099524 24.534894490078337 25.82509902972676 20.48908478009538 9 19.794209367998572 29.389355139313363 26.361445731943927 24.454989760744137 10-11 27.246212653600253 25.085482057470443 24.560381404886375 23.107923884042926 12-13 25.059533610596183 25.46717374253506 24.371850915445453 25.1014417314233 14-15 24.8414074395117 25.006011096106334 24.869240292694972 25.283341171686995 16-17 25.04237752256978 27.126234357523764 24.524040999903495 23.307347120002962 18-19 22.81730435304802 25.395186674530105 26.955138173880577 24.832370798541298 20-21 22.415956082668668 29.60656564386719 25.577804919593845 22.399673353870302 22-23 19.805610068690623 27.18079615380952 30.158189920879018 22.85540385662084 24-25 19.560419691485638 26.022371402361376 31.869457282231977 22.547751623921005 26-27 19.64148298956858 25.547280360514673 35.05428582256844 19.75695082734831 28-29 19.748086689417402 24.925609952008738 35.74623228928727 19.58007106928659 30-31 19.700775049825847 24.973687235849702 35.6900531695273 19.635484544797148 32-33 19.72745490329133 24.752457543031305 35.827613188544575 19.6924743651328 34-35 19.802960426227976 24.997591678573936 35.64123968065782 19.55820821454027 36-37 19.698636265689373 25.00939154873206 35.46612070935456 19.825851476223995 38-39 20.094379198789465 24.962160085181768 35.134900871957406 19.808559844071365 40-41 20.84004774535621 25.597452542341077 33.07973127480172 20.482768437500994 42-43 20.886355354593185 25.95631407979605 32.62761724892347 20.5297133166873 44-45 21.277947721662755 26.31961762077058 31.684164574387612 20.718270083179046 46-47 21.447973926952578 27.041931615170995 30.76530619654253 20.744788261333895 48-49 21.96410798805396 27.73526255084034 29.013841903962952 21.286787557142752 50-51 22.019199916502707 27.74014172376805 28.60338491880416 21.63727344092508 52-53 21.864876123656316 28.07291027185123 28.29993193776777 21.762281666724686 54-55 21.97608307096296 27.657482702964977 28.358838729023134 22.00759549704893 56-57 22.1074599214857 27.48196145838591 28.46693074252073 21.94364787760766 58-59 22.28213460166603 27.855625853188222 27.988788743688975 21.87345080145677 60-61 21.935588304676866 28.054338070015238 28.06849923526874 21.941574390039154 62-63 21.889523752025546 27.801311741845442 28.600306769420108 21.708857736708904 64-65 21.97370708089073 28.0950715792453 28.334424574660243 21.596796765203727 66-67 21.98847718437886 27.86844909446138 28.365713585540565 21.777360135619197 68-69 22.198436100886916 27.823752277757208 28.139567044193136 21.838244577162737 70-71 22.458237738323973 27.9060941880454 27.84213473331702 21.793533340313605 72-73 22.25703277110554 28.067009255742626 27.68571083053445 21.990247142617385 74-75 22.239265004229193 28.072291934662207 27.884288475086866 21.804154586021728 76-77 22.249566001859243 28.35894869174388 27.71681986109226 21.674665445304615 78-79 22.25943839840958 28.23832264554737 27.578960946177716 21.92327800986533 80-81 22.274002763404845 28.006968185783816 27.69132452724546 22.02770452356588 82-83 22.386245260942317 28.290415886711145 27.434750430997294 21.888588421349244 84-85 22.38363225407589 28.284097599698228 27.62704470931498 21.7052254369109 86-87 22.552430199157293 27.926700682590777 27.857373922734148 21.663495195517786 88-89 22.589650614296186 28.190301381957056 27.564356773624986 21.65569123012177 90-91 22.64147581957283 28.27776977117475 27.42128795928233 21.65946644997009 92-93 22.672114243139802 28.078809220516742 27.64241215014439 21.606664386199064 94-95 22.820430202397663 28.476443752175417 27.198567014448972 21.504559030977948 96-97 22.840483105475666 28.604420202592618 27.112871006587802 21.44222568534391 98-99 22.86795026827996 28.508196098276034 27.288237487420307 21.335616146023696 100-101 22.90954612341591 28.85644538656782 26.727404872563714 21.506603617452555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2102.0 1 2562.5 2 3136.0 3 3218.0 4 3114.5 5 2940.0 6 2759.0 7 2540.0 8 2289.5 9 2018.5 10 1745.5 11 1508.5 12 1334.0 13 1335.0 14 1402.5 15 1487.0 16 1990.5 17 2388.5 18 2746.0 19 3763.5 20 5753.5 21 8792.0 22 12736.5 23 18155.0 24 24724.0 25 33737.0 26 47037.5 27 67608.5 28 94611.0 29 132475.5 30 185859.0 31 239377.0 32 291989.5 33 364609.5 34 446328.0 35 530127.5 36 582601.0 37 599882.5 38 604269.0 39 602994.5 40 591900.5 41 554497.0 42 514700.0 43 504471.5 44 505355.0 45 500040.0 46 493721.0 47 486858.5 48 475847.0 49 450623.5 50 422568.5 51 402127.0 52 400902.0 53 394582.5 54 360403.0 55 329200.0 56 303950.0 57 263480.5 58 229524.5 59 206526.5 60 180506.0 61 152523.5 62 122607.0 63 92651.5 64 67357.5 65 47128.0 66 33721.5 67 24729.0 68 18354.5 69 14464.0 70 11957.0 71 9933.5 72 8471.5 73 6791.0 74 5127.0 75 4201.0 76 3418.5 77 2609.5 78 1810.0 79 1069.0 80 520.5 81 305.0 82 239.5 83 168.5 84 103.5 85 72.0 86 53.0 87 44.0 88 34.5 89 29.5 90 30.0 91 24.0 92 19.0 93 16.5 94 14.5 95 18.5 96 16.5 97 8.5 98 11.5 99 22.0 100 75.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5340486698989754 2 0.1171720728814536 3 0.14187225698374192 4 0.12929941778137471 5 0.12888928016959897 6 0.12731237176570254 7 0.13209259634433035 8 0.13126524978264476 9 0.13771431016091185 10-11 0.15635789315137366 12-13 0.15146806283166786 14-15 0.13997360269474554 16-17 0.12694466218273115 18-19 0.14529478464062928 20-21 0.15797369391500748 22-23 0.13691171328269547 24-25 0.15276918904833578 26-27 0.17756130054636693 28-29 0.14557410249692485 30-31 0.16916055084309797 32-33 0.14769196826807726 34-35 0.42576880472110823 36-37 0.1404084899899905 38-39 0.14328652461055488 40-41 0.14743740009525091 42-43 0.2555228034744313 44-45 0.14447097374801077 46-47 0.1796720949793853 48-49 0.28179282464248195 50-51 0.13422460479175086 52-53 0.16192303626288265 54-55 0.17636978007077703 56-57 0.18620601141526114 58-59 0.17978877205859733 60-61 0.18049590587200381 62-63 0.20766398698308075 64-65 0.16346812364517582 66-67 0.16016580873656755 68-69 0.16064312406061693 70-71 0.18117475433287406 72-73 0.1848589215007218 74-75 0.175606075552298 76-77 0.1698747559946385 78-79 0.17026367959201208 80-81 0.16364844276759447 82-83 0.17209515616873486 84-85 0.15848283026066015 86-87 0.15717816837492518 88-89 0.15313336296224012 90-91 0.1751888666023882 92-93 0.16366965678199666 94-95 0.17716176994179228 96-97 0.17391956140732356 98-99 0.17743048079088675 100-101 0.146899978397062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4141595E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.40071651645844 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95954079762276 27.244228870134613 2 12.222522012798258 8.653720737824882 3 4.083854919446043 4.337141708929608 4 1.7785437896024738 2.518468980312995 5 0.9962074921346261 1.7633229510314947 6 0.6472149178604141 1.3747123099439669 7 0.4355852363435891 1.079402062938776 8 0.3148482825597017 0.8916683837271844 9 0.2628502050733238 0.8374577036484329 >10 1.7539120955923877 12.117660297229344 >50 0.21476581232038358 5.410795299731708 >100 0.2973615723191039 21.848395196048568 >500 0.025737662753799052 6.1973200045634975 >1k 0.006729895480245594 3.336394438809523 >5k 1.4232229042531564E-4 0.36439224842703244 >10k+ 1.8298580197540583E-4 2.024918806698448 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 92099 0.6512631708092333 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 39630 0.2802371302529877 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 28102 0.1987187442434888 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTGGG 25277 0.17874221401475576 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 22381 0.15826361877850414 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 21967 0.1553360847910013 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 21230 0.15012450858619553 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 18788 0.13285630086280933 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4142676268129586E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 0.0 2 3.5356690670323964E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 0.0 3 4.949936693845355E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 0.0 4 6.364204320658314E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 0.0 5 7.778471947471272E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 7.071338134064793E-6 6 1.2021274827910148E-4 0.0 7.071338134064793E-6 7.071338134064793E-6 7.071338134064793E-6 7 1.2021274827910148E-4 0.0 7.071338134064793E-6 7.071338134064793E-6 7.071338134064793E-6 8 1.4849810081536064E-4 0.0 1.4142676268129586E-5 7.071338134064793E-6 7.071338134064793E-6 9 2.0506880588787898E-4 0.0 1.4142676268129586E-5 7.071338134064793E-6 7.071338134064793E-6 10-11 4.1013761177575796E-4 3.5356690670323965E-6 2.1214014402194376E-5 7.071338134064793E-6 7.071338134064793E-6 12-13 5.586357125911186E-4 1.4142676268129584E-5 2.8285352536259172E-5 7.071338134064793E-6 7.071338134064793E-6 14-15 6.540987774009933E-4 2.1214014402194376E-5 3.889235973735636E-5 7.071338134064793E-6 7.071338134064793E-6 16-17 7.601688494119652E-4 2.1214014402194376E-5 6.364204320658314E-5 7.071338134064793E-6 1.060700720109719E-5 18-19 8.945242739591963E-4 2.1214014402194376E-5 6.717771227361553E-5 7.071338134064793E-6 2.1214014402194376E-5 20-21 0.0010394867057075246 2.1214014402194376E-5 1.2021274827910146E-4 7.071338134064793E-6 2.1214014402194376E-5 22-23 0.0013612325908074726 2.1214014402194376E-5 4.8792233125047064E-4 7.071338134064793E-6 2.8285352536259172E-5 24-25 0.0018597619292590405 3.5356690670323964E-5 8.132038854174511E-4 7.071338134064793E-6 2.8285352536259172E-5 26-27 0.002605788102402876 3.889235973735636E-5 8.980599430262286E-4 7.071338134064793E-6 3.182102160329157E-5 28-29 0.0036877028369147894 4.242802880438875E-5 9.510949790317146E-4 7.071338134064793E-6 4.242802880438875E-5 30-31 0.005232790219207946 4.242802880438875E-5 0.0010112013531712654 7.071338134064793E-6 4.242802880438875E-5 32-33 0.0073188349687570604 4.242802880438875E-5 0.0010465580438415894 7.071338134064793E-6 6.364204320658314E-5 34-35 0.010235761949058786 4.242802880438875E-5 0.0010465580438415894 7.071338134064793E-6 7.071338134064793E-5 36-37 0.014761418354860254 4.242802880438875E-5 0.0010465580438415894 1.4142676268129586E-5 8.48560576087775E-5 38-39 0.020722556401876875 4.242802880438875E-5 0.0010465580438415894 1.4142676268129586E-5 8.48560576087775E-5 40-41 0.028426779298940465 4.242802880438875E-5 0.0010465580438415894 1.4142676268129586E-5 8.48560576087775E-5 42-43 0.03870496927680364 4.5963697871421154E-5 0.0010465580438415894 1.4142676268129586E-5 8.48560576087775E-5 44-45 0.05235972321368276 4.949936693845355E-5 0.0010465580438415894 1.4142676268129586E-5 8.48560576087775E-5 46-47 0.06937336276424264 4.949936693845355E-5 0.0010536293819756542 2.1214014402194376E-5 8.48560576087775E-5 48-49 0.0901807752237283 5.303503600548594E-5 0.0010536293819756542 2.1214014402194376E-5 8.48560576087775E-5 50-51 0.11568355620423298 5.6570705072518344E-5 0.0010536293819756542 2.1214014402194376E-5 8.48560576087775E-5 52-53 0.14627416497219725 6.0106374139550745E-5 0.0010536293819756542 2.1214014402194376E-5 9.89987338769071E-5 54-55 0.18262437865035733 7.071338134064793E-5 0.0010536293819756542 2.4749683469226774E-5 1.1314141014503669E-4 56-57 0.22428870293626707 7.424905040768032E-5 0.0010536293819756542 3.5356690670323964E-5 1.1314141014503669E-4 58-59 0.27321529148586143 7.778471947471272E-5 0.0010536293819756542 3.5356690670323964E-5 1.1667707921206908E-4 60-61 0.32959153475969294 7.778471947471272E-5 0.0010536293819756542 4.242802880438875E-5 1.2021274827910148E-4 62-63 0.393339648038287 7.778471947471272E-5 0.001060700720109719 4.242802880438875E-5 1.2374841734613388E-4 64-65 0.4655274033798875 8.132038854174511E-5 0.001060700720109719 4.242802880438875E-5 1.3435542454723107E-4 66-67 0.5468831486123029 8.48560576087775E-5 0.001060700720109719 5.303503600548594E-5 1.3435542454723107E-4 68-69 0.6365194307997082 9.54630648098747E-5 0.0010642363891767512 5.6570705072518344E-5 1.3789109361426346E-4 70-71 0.7372506425194612 9.89987338769071E-5 0.0010677720582437837 5.6570705072518344E-5 1.4142676268129586E-4 72-73 0.8488505009512718 9.89987338769071E-5 0.0010677720582437837 5.6570705072518344E-5 1.4142676268129586E-4 74-75 0.9721145316352222 1.1314141014503669E-4 0.0010677720582437837 5.6570705072518344E-5 1.4496243174832825E-4 76-77 1.1043485547422338 1.1314141014503669E-4 0.0010677720582437837 5.6570705072518344E-5 1.5556943894942543E-4 78-79 1.2510257859880727 1.1314141014503669E-4 0.0010677720582437837 7.071338134064793E-5 1.5556943894942543E-4 80-81 1.410289999112547 1.1314141014503669E-4 0.0010677720582437837 8.839172667580991E-5 1.6264077708349022E-4 82-83 1.5806880341291063 1.1314141014503669E-4 0.0010677720582437837 9.192739574284231E-5 1.69712115217555E-4 84-85 1.7652711734425997 1.1314141014503669E-4 0.0010677720582437837 9.89987338769071E-5 1.7324778428458743E-4 86-87 1.9647783718880367 1.1314141014503669E-4 0.0010677720582437837 1.0253440294393949E-4 1.87390460552717E-4 88-89 2.1789020262565852 1.1314141014503669E-4 0.0010677720582437837 1.0607007201097188E-4 1.944617986867818E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 79655 0.0 92.102486 1 TATCTCG 82775 0.0 88.55215 2 GTCGACG 60100 0.0 87.904236 1 CTCGACG 26330 0.0 81.650734 5 ATCTCGA 90995 0.0 80.4945 3 TCTCGAG 66315 0.0 77.79158 4 TCTCGAC 28120 0.0 76.72084 4 CTCGAGG 69015 0.0 74.65431 5 TCGAGGG 69775 0.0 73.74202 6 CGAGGGC 70170 0.0 73.32691 7 AGGGCGC 71880 0.0 71.595695 9 GAGGGCG 72590 0.0 70.86927 8 TCGACGG 84010 0.0 62.86412 2 CGACGGC 86890 0.0 60.772774 3 CGGCGCG 89785 0.0 58.63957 6 GACGGCG 90985 0.0 57.940556 4 ACGGCGC 90970 0.0 57.91543 5 GGTCGAC 1280 0.0 36.476067 1 GGGCGCG 71925 0.0 35.811176 10-11 GATCCTT 70450 0.0 35.472275 20-21 >>END_MODULE