FastQCFastQC Report
Tue 31 May 2016
SRR2048256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14141595
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT832560.5887313276896984No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT518380.3665640261936507No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT197850.13990642498247194No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG175770.12429291038245685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC292200.086.329351
GTCGACG174250.068.4124151
TCGACGG177850.067.983922
TATCTCG391200.064.474222
CGACGGC203250.059.5983543
GACGGCG248050.048.9684264
CGGCGCG249000.048.7035686
ACGGCGC250450.048.5560725
GGTCGAC12350.046.587751
ATCTCGA754500.045.237391
TCTCGAG774350.043.407162
CTCGAGG896700.037.4962653
TCGAGGG907400.037.054114
CGAGGGC929150.036.202075
GAGGGCG982650.034.2201846
AGGGCGC1030250.032.6662187
GGGCGCG1042750.032.263698
GGCGCGC1314900.025.9941399
AGTCGAC43150.021.929341
TCCATAT330800.021.49479718-19
Produced by FastQC (version 0.11.5)