##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14141595 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19722655047044 33.0 31.0 34.0 30.0 34.0 2 32.34929461634278 34.0 31.0 34.0 30.0 34.0 3 32.38743253501462 34.0 31.0 34.0 30.0 34.0 4 35.963872038479394 37.0 35.0 37.0 35.0 37.0 5 35.75327691112636 37.0 35.0 37.0 33.0 37.0 6 35.70389379698683 37.0 35.0 37.0 33.0 37.0 7 35.624087523366356 37.0 35.0 37.0 33.0 37.0 8 35.57582974197748 37.0 35.0 37.0 33.0 37.0 9 37.07732769889111 39.0 37.0 39.0 33.0 39.0 10-11 37.116206410945864 39.0 37.0 39.0 33.0 39.0 12-13 37.152013227645114 39.0 37.0 39.0 33.0 39.0 14-15 38.544370171822905 40.0 38.0 41.0 33.0 41.0 16-17 38.63224300370644 40.0 38.0 41.0 34.0 41.0 18-19 38.726236114101695 40.0 38.0 41.0 34.0 41.0 20-21 38.797844090429685 40.0 38.0 41.0 34.5 41.0 22-23 38.85528877753889 40.0 38.0 41.0 35.0 41.0 24-25 38.85878544817611 40.0 38.0 41.0 35.0 41.0 26-27 38.735761807632024 40.0 38.0 41.0 35.0 41.0 28-29 38.543096128831294 40.0 38.0 41.0 34.0 41.0 30-31 38.28813072358528 40.0 37.0 41.0 34.0 41.0 32-33 38.08289277128924 40.0 37.0 41.0 34.0 41.0 34-35 37.976500670539636 40.0 37.0 41.0 33.0 41.0 36-37 37.837730644952 40.0 36.0 41.0 33.0 41.0 38-39 37.67518999801649 40.0 36.0 41.0 33.0 41.0 40-41 37.36856786663739 40.0 36.0 41.0 33.0 41.0 42-43 37.07303288631869 40.0 36.0 41.0 31.5 41.0 44-45 36.6524847091152 40.0 36.0 41.0 30.5 41.0 46-47 36.03726711873732 40.0 35.0 41.0 29.0 41.0 48-49 35.67543813127161 40.0 35.0 41.0 27.0 41.0 50-51 35.55891573758123 40.0 35.0 41.0 26.5 41.0 52-53 35.39360457572148 39.0 35.0 41.0 26.0 41.0 54-55 35.15863108086464 39.0 35.0 41.0 25.0 41.0 56-57 34.95884191281111 39.0 35.0 41.0 25.0 41.0 58-59 34.74268118270959 39.0 35.0 41.0 24.5 41.0 60-61 34.491573475269234 38.0 34.5 40.5 24.0 41.0 62-63 34.21578241351135 37.5 34.0 40.0 23.0 41.0 64-65 33.946788993745045 37.0 34.0 40.0 23.0 41.0 66-67 33.6372383737478 37.0 34.0 40.0 22.0 41.0 68-69 33.329397002247624 36.0 34.0 39.0 22.0 41.0 70-71 33.00186658577056 36.0 33.5 39.0 21.0 41.0 72-73 32.66524097882876 35.0 33.0 38.5 20.0 40.5 74-75 32.40704036567304 35.0 33.0 37.5 20.0 40.0 76-77 32.19020966164001 35.0 33.0 37.0 20.0 39.0 78-79 31.934690853471622 35.0 33.0 36.5 20.0 39.0 80-81 31.667889124246592 35.0 33.0 36.0 19.5 38.5 82-83 31.422648859622978 35.0 33.0 36.0 19.0 37.0 84-85 31.215686066529273 35.0 33.0 35.5 18.0 37.0 86-87 31.030607403196033 35.0 33.0 35.0 18.0 36.5 88-89 30.88263042464446 35.0 33.0 35.0 16.5 36.0 90-91 30.7390934332372 35.0 33.0 35.0 13.0 36.0 92-93 30.584515608034312 35.0 32.5 35.0 7.5 36.0 94-95 30.460831292368365 35.0 32.0 35.0 2.0 35.5 96-97 30.330696784910046 35.0 32.0 35.0 2.0 35.0 98-99 30.177785037684927 35.0 32.0 35.0 2.0 35.0 100-101 29.671434268906726 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37526760598903053 1101 2 0.37741203689632385 1101 3 0.35228983503231603 1101 4 0.24051447862247954 1101 5 0.28049123984261115 1101 6 0.22032770427568948 1101 7 0.25273508701079805 1101 8 0.2584108755802461 1101 9 0.32458131776024146 1101 10-11 0.29113770604920575 1101 12-13 0.2746322633711813 1101 14-15 0.4064836305422901 1101 16-17 0.41917549183273195 1101 18-19 0.4353157258648679 1101 20-21 0.48566722599510115 1101 22-23 0.47930521236929025 1101 24-25 0.46340675475212123 1101 26-27 0.44505948702957454 1101 28-29 0.46867112140088096 1101 30-31 0.4526905426797825 1101 32-33 0.4403173843687682 1101 34-35 0.4830959328511284 1101 36-37 0.5337225579120215 1101 38-39 0.5264190985952126 1101 40-41 0.4692648158876125 1101 42-43 0.45440921146877855 1101 44-45 0.3512914560003111 1101 46-47 0.3042327632258761 1101 48-49 0.25295196661797803 1101 50-51 0.24613686773241739 1101 52-53 0.2179108003919623 1101 54-55 0.24372805343913484 1101 56-57 0.24224386005204934 1101 58-59 0.24764047778865717 1101 60-61 0.2646248321997504 1101 62-63 0.17757940448580456 1101 64-65 0.19556989719982454 1101 66-67 0.1726677528180076 1101 68-69 0.1776659507737861 1101 70-71 0.16600503259071786 1101 72-73 0.1341594472561809 1101 74-75 0.15333019790325864 1101 76-77 0.15473598188336268 1101 78-79 0.11819181967144132 1101 80-81 0.12553198188140868 1101 82-83 0.1173473787842525 1101 84-85 0.1258128309100428 1101 86-87 0.104395908049014 1101 88-89 0.12124179172487715 1101 90-91 0.11290897755424467 1101 92-93 0.1821099820617711 1101 94-95 0.1798041139936366 1101 96-97 0.21128991273465658 1101 98-99 0.2342222798482574 1101 100-101 0.31913149778027616 1102 1 0.34491176693769887 1102 2 0.3247117761084013 1102 3 0.3356154742126165 1102 4 0.22809589875611636 1102 5 0.2570922829252851 1102 6 0.2433668769782784 1102 7 0.2568524101770109 1102 8 0.24916292753101033 1102 9 0.27144860219691225 1102 10-11 0.2439977535952309 1102 12-13 0.2561016622557375 1102 14-15 0.3912385855694467 1102 16-17 0.42495132394108737 1102 18-19 0.4267981579841802 1102 20-21 0.4305188444264232 1102 22-23 0.43864234802391167 1102 24-25 0.4259683739015969 1102 26-27 0.39881671791917483 1102 28-29 0.43152483864533764 1102 30-31 0.431097833779468 1102 32-33 0.46736042351879803 1102 34-35 0.4796258045253694 1102 36-37 0.4991038142849007 1102 38-39 0.505669132346469 1102 40-41 0.4486749506200667 1102 42-43 0.43850107073448896 1102 44-45 0.39631683487573355 1102 46-47 0.43231355681097483 1102 48-49 0.3797744137598613 1102 50-51 0.3233844353129314 1102 52-53 0.34401261197807287 1102 54-55 0.36265269278371903 1102 56-57 0.3670126842706196 1102 58-59 0.32121906188015004 1102 60-61 0.32058493582288605 1102 62-63 0.3012285667818162 1102 64-65 0.29833824828467215 1102 66-67 0.24177149563159617 1102 68-69 0.28255404348296054 1102 70-71 0.27114163387384593 1102 72-73 0.28080658206152975 1102 74-75 0.30431544997155413 1102 76-77 0.2909958511379642 1102 78-79 0.2717224402564007 1102 80-81 0.2829846131806981 1102 82-83 0.2726526895292096 1102 84-85 0.2914545585150421 1102 86-87 0.28533815270996854 1102 88-89 0.27736092673843515 1102 90-91 0.26064778501375585 1102 92-93 0.2929783650716793 1102 94-95 0.2854447168031733 1102 96-97 0.3393934634655196 1102 98-99 0.3498216982373208 1102 100-101 0.4874731694856038 1103 1 0.2628327567962856 1103 2 0.29629797811679026 1103 3 0.251936677197115 1103 4 0.16128761689572713 1103 5 0.13842714793004518 1103 6 0.17624503142604198 1103 7 0.1541553745307951 1103 8 0.15064937742025108 1103 9 0.17934539289272067 1103 10-11 0.14958992689485484 1103 12-13 0.18238037879454794 1103 14-15 0.28390071007387974 1103 16-17 0.3144969680375169 1103 18-19 0.33943527544442986 1103 20-21 0.37049024013924736 1103 22-23 0.36491459490492417 1103 24-25 0.37364474746161846 1103 26-27 0.34142777884442665 1103 28-29 0.3731232907690938 1103 30-31 0.30683711959436266 1103 32-33 0.3471316215460547 1103 34-35 0.3735803517109062 1103 36-37 0.40115712487862965 1103 38-39 0.3987600651564591 1103 40-41 0.3521981971953352 1103 42-43 0.3272425719942831 1103 44-45 0.2549942917141266 1103 46-47 0.2950099216607853 1103 48-49 0.24307999292188498 1103 50-51 0.18223204981418917 1103 52-53 0.15195559513398393 1103 54-55 0.17511563676870168 1103 56-57 0.20697847860940044 1103 58-59 0.18158323424599843 1103 60-61 0.16645417139199736 1103 62-63 0.12279014539530664 1103 64-65 0.1148689377033918 1103 66-67 0.10029652384019982 1103 68-69 0.1275788896115131 1103 70-71 0.120023402364005 1103 72-73 0.1373408597572734 1103 74-75 0.10502222469424538 1103 76-77 0.0763553246257942 1103 78-79 0.10447154838976402 1103 80-81 0.10531346324409085 1103 82-83 0.1300830726372375 1103 84-85 0.10689656520563773 1103 86-87 0.13121279617684678 1103 88-89 0.1440516894591788 1103 90-91 0.12238381386795893 1103 92-93 0.12693716944305322 1103 94-95 0.1537089972750607 1103 96-97 0.14801103467858923 1103 98-99 0.2292563749613592 1103 100-101 0.2859683900789918 1104 1 0.2234319425050444 1104 2 0.223246092407841 1104 3 0.21951285586654024 1104 4 0.15815303700534145 1104 5 0.14930824303711887 1104 6 0.15187765002523435 1104 7 0.18202531748477213 1104 8 0.1558925539456979 1104 9 0.17712637367890238 1104 10-11 0.20952280825773073 1104 12-13 0.2199557676191617 1104 14-15 0.28994193394624546 1104 16-17 0.29871655673041175 1104 18-19 0.29648900580788506 1104 20-21 0.2812656015578554 1104 22-23 0.2800166464481819 1104 24-25 0.27854732686812866 1104 26-27 0.23322616320975698 1104 28-29 0.22599782227900533 1104 30-31 0.25528567014922743 1104 32-33 0.31757664360082316 1104 34-35 0.3263656646354036 1104 36-37 0.29966872558679114 1104 38-39 0.33383334098981265 1104 40-41 0.16835248420922255 1104 42-43 0.24439833648231257 1104 44-45 0.24385782227788866 1104 46-47 0.24952199565488087 1104 48-49 0.15200639538850425 1104 50-51 0.09297796270058711 1104 52-53 0.11614350552060415 1104 54-55 0.10115592491764147 1104 56-57 0.08431621710558801 1104 58-59 0.15159207467276303 1104 60-61 0.15514948153877128 1104 62-63 0.12612602636131243 1104 64-65 0.12405868097834372 1104 66-67 0.14262387091630302 1104 68-69 0.1729897807501004 1104 70-71 0.13425068468141177 1104 72-73 0.12846985555120227 1104 74-75 0.13046783170172205 1104 76-77 0.11650160261599751 1104 78-79 0.10977744130554257 1104 80-81 0.10880742960180001 1104 82-83 0.08994124063458742 1104 84-85 0.13993278336858594 1104 86-87 0.10452418921412487 1104 88-89 0.12659016750104612 1104 90-91 0.1308165818176299 1104 92-93 0.14630435581397094 1104 94-95 0.14890677139841202 1104 96-97 0.18366861623659503 1104 98-99 0.1714730127238937 1104 100-101 0.21901402049461893 1105 1 0.18068814350324658 1105 2 0.20485981490024585 1105 3 0.1891532583229747 1105 4 0.0991531534245027 1105 5 0.11433855584651553 1105 6 0.10792653118109286 1105 7 0.1358715450087189 1105 8 0.10464849628127837 1105 9 0.11889555992971879 1105 10-11 0.1033437183198842 1105 12-13 0.0879277421846183 1105 14-15 0.20104639770588761 1105 16-17 0.1579996404844124 1105 18-19 0.1522956584422417 1105 20-21 0.2149401930406114 1105 22-23 0.18880665464251223 1105 24-25 0.188367455345535 1105 26-27 0.17313996045786695 1105 28-29 0.16226227132709425 1105 30-31 0.18199921604193747 1105 32-33 0.20060332570720618 1105 34-35 0.21143021308520815 1105 36-37 0.19437988021825703 1105 38-39 0.20308846807887448 1105 40-41 0.22867015505700294 1105 42-43 0.2706418514167339 1105 44-45 0.18509613066417785 1105 46-47 0.22707721803055136 1105 48-49 0.18968473820673637 1105 50-51 0.16001477821875199 1105 52-53 0.23600128160695277 1105 54-55 0.21790759384363412 1105 56-57 0.21193141248507885 1105 58-59 0.14279252228575956 1105 60-61 0.1731186223930834 1105 62-63 0.16664573925214654 1105 64-65 0.1191628747833775 1105 66-67 0.10064338416639629 1105 68-69 0.09268306055121656 1105 70-71 0.1252815157801095 1105 72-73 0.09396742273720804 1105 74-75 0.13048311606750929 1105 76-77 0.16291610419017388 1105 78-79 0.18621255510450752 1105 80-81 0.13201796310199398 1105 82-83 0.11266836506056421 1105 84-85 0.13614814989727364 1105 86-87 0.12577608780951977 1105 88-89 0.12285533932623949 1105 90-91 0.13957039045663677 1105 92-93 0.10560098728703693 1105 94-95 0.11515942508477295 1105 96-97 0.10560750389253926 1105 98-99 0.11456616336597847 1105 100-101 0.11337070459571308 1106 1 0.16240643881803862 1106 2 0.18253183338721612 1106 3 0.14471528402178535 1106 4 0.09238655006861052 1106 5 0.08163927632119083 1106 6 0.06472797269307051 1106 7 0.08430162924246787 1106 8 0.10612869508002376 1106 9 0.1413638234412744 1106 10-11 0.1369882455909064 1106 12-13 0.12607142850576736 1106 14-15 0.16509457608332667 1106 16-17 0.17801897069057304 1106 18-19 0.172308253490165 1106 20-21 0.20864489543403408 1106 22-23 0.2047065100967913 1106 24-25 0.19306965072407678 1106 26-27 0.19764601922673108 1106 28-29 0.1279040934203053 1106 30-31 0.1451352982356795 1106 32-33 0.13744421971372844 1106 34-35 0.20150760993385575 1106 36-37 0.13695305402347202 1106 38-39 0.24155733828106207 1106 40-41 0.1520796636196451 1106 42-43 0.22544942936978174 1106 44-45 0.17229669161268646 1106 46-47 0.2330112721244788 1106 48-49 0.1694844281288468 1106 50-51 0.14486719808732573 1106 52-53 0.11239164953160241 1106 54-55 0.17127896078666538 1106 56-57 0.17188189427282907 1106 58-59 0.1930147225339809 1106 60-61 0.20119624367222144 1106 62-63 0.184465941057681 1106 64-65 0.19070289930470352 1106 66-67 0.19392397955680707 1106 68-69 0.20674588843714048 1106 70-71 0.154466421235135 1106 72-73 0.1494579060405954 1106 74-75 0.16999498226000043 1106 76-77 0.1468577956170023 1106 78-79 0.1476928670332036 1106 80-81 0.14303315475405043 1106 82-83 0.09955874198556458 1106 84-85 0.11969035065565947 1106 86-87 0.08239672635015438 1106 88-89 0.10738891999679012 1106 90-91 0.11756770500537428 1106 92-93 0.14007609418534628 1106 94-95 0.1787833260307785 1106 96-97 0.16531677209330553 1106 98-99 0.17987316352787985 1106 100-101 0.23232611365436284 1107 1 0.02848936469862906 1107 2 0.07205734425268417 1107 3 0.0876509532061931 1107 4 0.07572586265934689 1107 5 -0.042646934483748566 1107 6 0.02405035354949092 1107 7 0.04430783741788247 1107 8 0.06963062391859154 1107 9 0.11305986353261943 1107 10-11 0.05148429530769505 1107 12-13 0.09225865402070355 1107 14-15 0.1255671190701193 1107 16-17 0.09963999536413581 1107 18-19 0.13609101906497756 1107 20-21 0.14890323991015464 1107 22-23 0.12491246506915843 1107 24-25 0.08477889423457441 1107 26-27 0.11664128482723157 1107 28-29 0.10149596479650569 1107 30-31 0.08828637514623239 1107 32-33 0.052041495278231764 1107 34-35 0.11110825193514273 1107 36-37 0.15227814980556786 1107 38-39 0.1732200136814015 1107 40-41 0.14136080285599917 1107 42-43 0.16438436384174082 1107 44-45 0.11542840516561625 1107 46-47 0.14599795164949825 1107 48-49 0.16238931640082654 1107 50-51 0.06799024768508843 1107 52-53 0.0979539479532292 1107 54-55 0.1516443768968756 1107 56-57 0.17180690860749337 1107 58-59 0.09658549912888503 1107 60-61 0.12497117257874635 1107 62-63 0.09513666046078129 1107 64-65 0.06600935216045656 1107 66-67 0.01220799768648817 1107 68-69 0.05096022142043921 1107 70-71 -0.021539373680880658 1107 72-73 -0.02071357512858185 1107 74-75 0.04674605575048929 1107 76-77 0.04938820611144479 1107 78-79 0.0570649796345748 1107 80-81 0.05757010248177252 1107 82-83 0.02044715376522177 1107 84-85 0.00987977791086081 1107 86-87 -9.829592579606583E-4 1107 88-89 0.00978535162909111 1107 90-91 -0.018283569451725157 1107 92-93 -1.471214394399567E-5 1107 94-95 -0.054141772340837946 1107 96-97 -0.04823383998018116 1107 98-99 -0.03243925503982581 1107 100-101 0.016834336849161247 1108 1 0.09950666516009221 1108 2 0.10685165729037749 1108 3 0.1041339423108667 1108 4 0.04079042570442937 1108 5 0.005550365933117973 1108 6 0.007024040996675751 1108 7 0.051345956546050786 1108 8 0.04584313559824693 1108 9 0.06842844011117677 1108 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-0.2725214107607705 2214 94-95 -0.26358127850241786 2214 96-97 -0.367821832128687 2214 98-99 -0.42088615270436236 2214 100-101 -0.4351437518958008 2215 1 -0.3545631999018255 2215 2 -0.35347554967227524 2215 3 -0.33069357587584136 2215 4 -0.20771888961640883 2215 5 -0.2531957658367574 2215 6 -0.21962455756506927 2215 7 -0.2667893939741859 2215 8 -0.2688389209694364 2215 9 -0.34180636357262273 2215 10-11 -0.3008947492780649 2215 12-13 -0.30884564072307796 2215 14-15 -0.39954916710045296 2215 16-17 -0.44349272571383835 2215 18-19 -0.5249638208337046 2215 20-21 -0.4312863767571713 2215 22-23 -0.4279359522042867 2215 24-25 -0.44893509637203977 2215 26-27 -0.4371298821867171 2215 28-29 -0.46423740029086247 2215 30-31 -0.4514271034508468 2215 32-33 -0.49981536481499234 2215 34-35 -0.5334822681823113 2215 36-37 -0.45287986467374 2215 38-39 -0.4670472685751932 2215 40-41 -0.42150953968637594 2215 42-43 -0.3770361566255289 2215 44-45 -0.46158453600318694 2215 46-47 -0.34127258017360873 2215 48-49 -0.26130265136749387 2215 50-51 -0.2706065085107383 2215 52-53 -0.3169611990874728 2215 54-55 -0.30726102541405 2215 56-57 -0.3396895134525124 2215 58-59 -0.3068622795186968 2215 60-61 -0.22814726405867702 2215 62-63 -0.23922306135597893 2215 64-65 -0.3011636066726098 2215 66-67 -0.2878650674120493 2215 68-69 -0.28862793603595094 2215 70-71 -0.28222496139864717 2215 72-73 -0.24724076869788547 2215 74-75 -0.2077549166477013 2215 76-77 -0.22938091457659482 2215 78-79 -0.21107737378667224 2215 80-81 -0.23636639159551365 2215 82-83 -0.1929246163459588 2215 84-85 -0.20647445685407817 2215 86-87 -0.24910063738930432 2215 88-89 -0.25408917758561955 2215 90-91 -0.21957801060703375 2215 92-93 -0.3222533154956366 2215 94-95 -0.32236233812237813 2215 96-97 -0.361760973027792 2215 98-99 -0.39113268697024495 2215 100-101 -0.47547292883174563 2216 1 -0.7931717897299286 2216 2 -0.6556577271593333 2216 3 -1.625600302489886 2216 4 -0.6764165272164888 2216 5 -0.5167162516133388 2216 6 -0.5651156865927831 2216 7 -0.56651050345139 2216 8 -0.5411856579503223 2216 9 -0.6967416694225648 2216 10-11 -0.6825156173318518 2216 12-13 -0.5579459370794737 2216 14-15 -0.8371826965022748 2216 16-17 -0.8372930809471271 2216 18-19 -0.8761614302630178 2216 20-21 -1.0575376514840542 2216 22-23 -0.783883010895245 2216 24-25 -0.7678884892583611 2216 26-27 -0.6466427604496019 2216 28-29 -0.6525903052596291 2216 30-31 -0.6570782635227417 2216 32-33 -0.7738417129267035 2216 34-35 -0.8010075091831652 2216 36-37 -0.8253780194889728 2216 38-39 -0.8229967569941934 2216 40-41 -0.7408549639098467 2216 42-43 -0.756850011286943 2216 44-45 -0.6586766797416743 2216 46-47 -0.3690644081178718 2216 48-49 -0.3858976614807972 2216 50-51 -0.5686241321381118 2216 52-53 -0.6360403392031984 2216 54-55 -0.5864477989605774 2216 56-57 -0.5887481522977325 2216 58-59 -0.5229461635903832 2216 60-61 -0.3687186163678362 2216 62-63 -0.32442330615988624 2216 64-65 -0.4127206961260441 2216 66-67 -0.3934913667883464 2216 68-69 -0.310759483624814 2216 70-71 -0.3601167603270241 2216 72-73 -0.3657293088327691 2216 74-75 -0.430671195966422 2216 76-77 -0.35521124772400015 2216 78-79 -0.17919736995544966 2216 80-81 -0.49046442724842976 2216 82-83 -0.34019701773421573 2216 84-85 -0.35114983110262443 2216 86-87 -0.3332829317850141 2216 88-89 -0.2218569688290195 2216 90-91 -0.25017547016790687 2216 92-93 -0.19791571570072009 2216 94-95 -0.3215967272957947 2216 96-97 -0.4572648141108999 2216 98-99 -0.3324937008922362 2216 100-101 -0.4977219132758499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 253.0 3 88.0 4 135.0 5 291.0 6 767.0 7 1854.0 8 4425.0 9 9266.0 10 17160.0 11 22566.0 12 25089.0 13 38030.0 14 66407.0 15 118909.0 16 150157.0 17 232985.0 18 206906.0 19 93600.0 20 61402.0 21 49758.0 22 46709.0 23 47941.0 24 55257.0 25 69850.0 26 91324.0 27 120171.0 28 152149.0 29 187289.0 30 230543.0 31 293453.0 32 381787.0 33 514877.0 34 711825.0 35 988150.0 36 1544777.0 37 2893760.0 38 3655141.0 39 1055659.0 40 885.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.67536969122886 42.76857331196715 12.979169671610322 21.57688732519367 2 22.572644239497258 27.889257797595924 33.97511284200465 15.562985120902173 3 19.81030434266705 28.753850194328834 25.768225054580512 25.66762040842361 4 22.23058997234753 26.924607867782946 28.82353086762844 22.021271292241078 5 22.033450989956982 27.90154238774751 25.43279701308272 24.632209609212786 6 24.10909856945633 26.052227144852015 27.394334609480183 22.444339676211474 7 23.62546813195144 29.041322903167 24.202961110060563 23.130247854821 8 26.20224239203569 25.70226342926664 26.49628984566451 21.599204333033157 9 23.309966096469317 26.285479113211768 27.132215284060955 23.272339506257957 10-11 25.548734071368894 25.735997247835197 25.322985137107946 23.392283543687963 12-13 24.273657600702155 26.167457884779093 25.38789216192534 24.170992352593412 14-15 23.563731672417433 25.7495742170526 26.040711815039252 24.645982295490715 16-17 23.63369549191587 26.336477603834645 25.91214781642382 24.117679087825667 18-19 22.82255997290263 26.88834958150053 27.782460182178887 22.506630263417954 20-21 20.988983208754032 27.06744536242199 29.035770010384258 22.907801418439718 22-23 20.15077507169453 26.209921865249285 30.56565048002011 23.073652583036072 24-25 19.957543685843074 25.605488631232898 33.15240607583515 21.284561607088875 26-27 20.056408528545777 25.19904592179372 34.43304835709259 20.311497192567916 28-29 20.168672628511846 25.533735763186545 34.20320338688811 20.0943882214135 30-31 20.03812302780226 25.585924462210173 34.18080676110964 20.19514574887792 32-33 20.090198453569062 25.382695516311987 34.309075461431334 20.21803056868762 34-35 20.239962323910422 25.636906586562546 34.00091361688692 20.12221747264011 36-37 20.164244354659388 25.708621858075475 33.805480961547964 20.32165282571718 38-39 20.390907107720167 25.61214629608612 33.72634416414839 20.270602432045322 40-41 20.696226525436067 25.95801013441676 32.749180999195474 20.596582340951695 42-43 20.854320998828904 26.23035375249241 32.11242758075362 20.802897667925073 44-45 21.033437175933834 26.296598792427588 31.664833422255413 21.005130609383173 46-47 21.644583843037136 26.96221791036987 30.120915330558546 21.27228291603445 48-49 22.03429316141496 27.51367861970308 28.662693281769137 21.789334937112823 50-51 22.00275407571845 27.36434133468688 28.520937166420474 22.111967423174196 52-53 21.986473237283345 27.64625206704053 28.18146043639349 22.185814259282637 54-55 22.069660459092486 27.483890607813333 28.11265985201811 22.333789081076073 56-57 22.07831931263765 27.42512425225019 28.22110235797306 22.275454077139106 58-59 22.147593676668016 27.769424170328737 27.851589583777503 22.23139256922575 60-61 21.887969497075822 27.84528902150005 27.981175390753304 22.28556609067082 62-63 21.850233301123385 27.624578415659617 28.386454993230963 22.138733289986032 64-65 21.919171776592385 27.963171763863976 28.137929278840186 21.97972718070345 66-67 21.892838194110666 27.81938886249303 28.192537993234257 22.095234950162045 68-69 22.04086950587964 27.837814617092345 27.99091969470205 22.130396182325967 70-71 22.127268784025176 27.983132826095243 27.844459918151838 22.04513847172775 72-73 21.922088703572687 28.10288019137869 27.74002861770543 22.235002487343188 74-75 21.871416598640376 28.110473700556486 27.869539016540713 22.148570684262424 76-77 21.92121709151009 28.26826488478217 27.673681499224006 22.136836524483737 78-79 21.864033017492016 28.229888495604634 27.528903210705725 22.37717527619763 80-81 21.833289927096615 28.108587200890312 27.644618961951455 22.413503910061618 82-83 21.861148193537133 28.382953502709697 27.41479192743056 22.341106376322614 84-85 21.871882735540428 28.286666508031978 27.61215204420774 22.229298712219855 86-87 22.025015303401943 28.059800882702135 27.685599867368747 22.229583946527175 88-89 22.07575236437981 28.32451134076373 27.37468277880674 22.225053516049723 90-91 22.17021011796419 28.37973550338788 27.23097772667552 22.21907665197241 92-93 22.161433828398792 28.18199162365908 27.36729711147271 22.28927743646942 94-95 22.365048431234055 28.504720364545726 26.960320567507985 22.16991063671223 96-97 22.322724559086392 28.491843255152784 26.96633865495694 22.21909353080389 98-99 22.296690117589325 28.240912089800368 27.2596327569955 22.202765035614807 100-101 22.247208959840332 28.490732455435076 26.852847738521696 22.4092108462029 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 354.0 1 293.5 2 204.0 3 151.0 4 131.5 5 129.5 6 129.5 7 123.0 8 120.5 9 122.5 10 148.5 11 185.0 12 231.5 13 524.5 14 774.0 15 948.5 16 6394.0 17 7927.5 18 4335.0 19 5067.5 20 6947.5 21 9678.0 22 13507.0 23 18990.5 24 25160.5 25 33679.0 26 46402.5 27 66442.0 28 93739.0 29 132954.0 30 186890.5 31 238369.5 32 286750.0 33 356370.0 34 434319.0 35 513866.0 36 562991.5 37 580945.5 38 591336.0 39 595901.5 40 585799.0 41 548712.0 42 512625.5 43 506750.5 44 511148.0 45 506907.0 46 500661.5 47 495521.5 48 485974.5 49 460252.5 50 432678.0 51 412784.5 52 412326.0 53 405157.0 54 368718.5 55 336910.0 56 311624.5 57 269999.5 58 234795.5 59 210735.5 60 183615.5 61 154982.0 62 124427.0 63 93187.0 64 66724.0 65 46089.0 66 32507.5 67 23724.5 68 17580.5 69 13784.0 70 11410.0 71 9275.0 72 7898.5 73 6290.0 74 4539.0 75 3595.0 76 2958.5 77 2444.0 78 1628.0 79 792.0 80 306.0 81 149.5 82 111.5 83 48.5 84 20.0 85 14.5 86 11.0 87 6.0 88 4.5 89 5.5 90 6.0 91 5.0 92 3.5 93 2.5 94 3.0 95 3.0 96 1.5 97 0.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08557026276031805 2 0.011964704122837629 3 7.14205151540544E-4 4 0.0 5 0.00514793416159917 6 0.002319398907973252 7 0.005777283255530936 8 0.0 9 0.0 10-11 0.0 12-13 2.4749683469226777E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.005837389629670486 28-29 0.0 30-31 7.778471947471272E-5 32-33 0.0 34-35 0.0 36-37 3.0760320883181847E-4 38-39 0.0 40-41 0.001965832001270012 42-43 0.0010889860726459781 44-45 0.0 46-47 0.001499123684421736 48-49 0.0 50-51 6.010637413955073E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.5256564058014675E-4 68-69 0.0 70-71 3.1113887789885087E-4 72-73 0.0 74-75 2.1214014402194376E-5 76-77 8.839172667580991E-5 78-79 0.0 80-81 1.4142676268129586E-5 82-83 3.57102575770272E-4 84-85 6.328847629987989E-4 86-87 9.298809646295201E-4 88-89 3.040675397647861E-4 90-91 1.0607007201097188E-4 92-93 6.929911371383497E-4 94-95 3.5003123763620726E-4 96-97 5.480287053900215E-4 98-99 3.5356690670323964E-5 100-101 6.576344464680257E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4141595E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.20821729879376 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86318678340228 24.112097876000057 2 13.13217975861199 8.45928198544392 3 4.535323672848101 4.382240711263652 4 1.9945708728183884 2.5696628835831756 5 1.1311448253104965 1.8216079165003292 6 0.6853832682213784 1.3244983941498962 7 0.4716246178777592 1.063313172324721 8 0.36887487575202 0.9504641723429288 9 0.2829933902765512 0.820324134733456 >10 1.9075239932463572 12.044120247401887 >50 0.23895160138599678 5.461858305145446 >100 0.34671759052697354 23.493876153177247 >500 0.03129606905740637 6.902420843307497 >1k 0.009832734967816852 4.566411407377647 >5k 2.6396379773618503E-4 0.6204616089575082 >10k+ 1.3198189886809252E-4 1.4073601882908018 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 83256 0.5887313276896984 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51838 0.3665640261936507 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 19785 0.13990642498247194 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 17577 0.12429291038245685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.071338134064793E-6 7.071338134064793E-6 0.0 0.0 7.071338134064793E-6 2 2.1214014402194376E-5 7.071338134064793E-6 0.0 0.0 7.071338134064793E-6 3 2.8285352536259172E-5 7.071338134064793E-6 0.0 0.0 7.071338134064793E-6 4 3.5356690670323964E-5 7.071338134064793E-6 0.0 0.0 7.071338134064793E-6 5 6.364204320658314E-5 7.071338134064793E-6 0.0 7.071338134064793E-6 7.071338134064793E-6 6 1.0607007201097188E-4 1.4142676268129586E-5 0.0 7.071338134064793E-6 7.071338134064793E-6 7 1.1314141014503669E-4 2.1214014402194376E-5 0.0 1.4142676268129586E-5 7.071338134064793E-6 8 1.4849810081536064E-4 2.1214014402194376E-5 0.0 1.4142676268129586E-5 7.071338134064793E-6 9 1.909261296197494E-4 2.1214014402194376E-5 0.0 1.4142676268129586E-5 7.071338134064793E-6 10-11 4.348872952449847E-4 2.1214014402194376E-5 0.0 1.4142676268129586E-5 1.4142676268129586E-5 12-13 6.434917701998961E-4 2.1214014402194376E-5 0.0 1.4142676268129586E-5 1.4142676268129586E-5 14-15 8.202752235515159E-4 2.8285352536259172E-5 0.0 1.4142676268129586E-5 1.4142676268129586E-5 16-17 9.687733243668767E-4 3.5356690670323964E-5 0.0 1.4142676268129586E-5 1.4142676268129586E-5 18-19 0.0011455567777184964 4.242802880438876E-5 0.0 1.4142676268129586E-5 1.4142676268129586E-5 20-21 0.0013400185764052782 1.0607007201097188E-4 0.0 1.4142676268129586E-5 1.7678345335161982E-5 22-23 0.0016653001305722587 4.6670831684827627E-4 0.0 1.4142676268129586E-5 2.8285352536259172E-5 24-25 0.002160293799956794 7.813828638141596E-4 0.0 1.4142676268129586E-5 2.8285352536259172E-5 26-27 0.002969962016307213 8.556319142218399E-4 0.0 1.4142676268129586E-5 2.8285352536259172E-5 28-29 0.0040907691105564825 9.157382883613907E-4 0.0 1.4142676268129586E-5 2.8285352536259172E-5 30-31 0.005710105543257319 9.758446625009415E-4 0.0 1.4142676268129586E-5 2.8285352536259172E-5 32-33 0.007859792336013017 0.0010041300150372006 0.0 1.4142676268129586E-5 2.8285352536259172E-5 34-35 0.010797933330716939 0.0010112013531712654 0.0 1.4142676268129586E-5 2.8285352536259172E-5 36-37 0.015337732412786534 0.0010112013531712654 0.0 1.4142676268129586E-5 2.8285352536259172E-5 38-39 0.02139786919368006 0.0010112013531712654 0.0 2.1214014402194376E-5 2.8285352536259172E-5 40-41 0.02921169783182166 0.0010112013531712654 0.0 2.1214014402194376E-5 2.8285352536259172E-5 42-43 0.039578279536360644 0.0010112013531712654 0.0 2.1214014402194376E-5 3.5356690670323964E-5 44-45 0.05329313984737931 0.0010112013531712654 0.0 2.1214014402194376E-5 3.889235973735636E-5 46-47 0.0705259908800952 0.0010218083603723625 0.0 2.1214014402194376E-5 4.5963697871421154E-5 48-49 0.09170818426068629 0.001035951036640492 0.0 2.1214014402194376E-5 4.949936693845355E-5 50-51 0.11769181623430738 0.0010394867057075246 0.0 2.1214014402194376E-5 5.303503600548594E-5 52-53 0.1486572059233771 0.0010394867057075246 0.0 2.4749683469226774E-5 6.364204320658314E-5 54-55 0.18531148714130197 0.0010394867057075246 0.0 3.5356690670323964E-5 6.717771227361553E-5 56-57 0.22717734456403255 0.0010394867057075246 0.0 3.5356690670323964E-5 7.071338134064793E-5 58-59 0.2761640394877664 0.0010394867057075246 0.0 3.889235973735636E-5 7.071338134064793E-5 60-61 0.33263928149547484 0.0010394867057075246 0.0 5.303503600548594E-5 7.071338134064793E-5 62-63 0.39649346484607995 0.0010394867057075246 0.0 6.0106374139550745E-5 7.071338134064793E-5 64-65 0.46874132656181994 0.0010465580438415894 0.0 7.071338134064793E-5 7.071338134064793E-5 66-67 0.5502597125713189 0.0010465580438415894 0.0 7.778471947471272E-5 7.071338134064793E-5 68-69 0.6400126718379362 0.0010465580438415894 0.0 7.778471947471272E-5 7.778471947471272E-5 70-71 0.7408287396152979 0.0010465580438415894 0.0 7.778471947471272E-5 8.48560576087775E-5 72-73 0.8522270649102877 0.0010465580438415894 0.0 8.839172667580991E-5 8.839172667580991E-5 74-75 0.9754097752056963 0.0010465580438415894 0.0 9.54630648098747E-5 9.89987338769071E-5 76-77 1.1073043740822728 0.0010465580438415894 0.0 1.0253440294393949E-4 1.1314141014503667E-4 78-79 1.2538649282488996 0.0010465580438415894 0.0 1.1314141014503669E-4 1.2021274827910148E-4 80-81 1.4127402177760007 0.0010465580438415894 0.0 1.2728408641316628E-4 1.2021274827910148E-4 82-83 1.5827422578570522 0.0010536293819756542 0.0 1.3435542454723107E-4 1.2021274827910148E-4 84-85 1.7668551531846302 0.001060700720109719 0.0 1.4496243174832825E-4 1.2728408641316628E-4 86-87 1.9657683592268058 0.001060700720109719 0.0 1.5203376988239304E-4 1.2728408641316628E-4 88-89 2.1790717383718032 0.0010677720582437837 0.0 1.5556943894942543E-4 1.3789109361426346E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 29220 0.0 86.32935 1 GTCGACG 17425 0.0 68.412415 1 TCGACGG 17785 0.0 67.98392 2 TATCTCG 39120 0.0 64.47422 2 CGACGGC 20325 0.0 59.598354 3 GACGGCG 24805 0.0 48.968426 4 CGGCGCG 24900 0.0 48.703568 6 ACGGCGC 25045 0.0 48.556072 5 GGTCGAC 1235 0.0 46.58775 1 ATCTCGA 75450 0.0 45.23739 1 TCTCGAG 77435 0.0 43.40716 2 CTCGAGG 89670 0.0 37.496265 3 TCGAGGG 90740 0.0 37.05411 4 CGAGGGC 92915 0.0 36.20207 5 GAGGGCG 98265 0.0 34.220184 6 AGGGCGC 103025 0.0 32.666218 7 GGGCGCG 104275 0.0 32.26369 8 GGCGCGC 131490 0.0 25.994139 9 AGTCGAC 4315 0.0 21.92934 1 TCCATAT 33080 0.0 21.494797 18-19 >>END_MODULE