FastQCFastQC Report
Tue 31 May 2016
SRR2048255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488867
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1674261.1555492917424117No Hit
AATTCCCCTTCAGTAGAAGGGCACACCGGTATCCCCGCCCGACAACGACG744570.5138911137772194No Hit
AATTCCCCTTGGTTGCAAGGGAACGATGCGTAATGTGTGTATTGCCGTTG732390.505484659359493No Hit
AATTCGACTGTCACCGAAGTGCGGCAAAGACAGCAACGGCAATACACACA707490.48829905057448597No Hit
AATTCCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGG601760.4153257808219235No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG598140.4128273107897256No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT583520.4027368047480869No Hit
AATTCCCCTTCTACTGAAGGGACAGGTGTTCTCCCGTATTGTTGACATGC502090.34653503272547126No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG460540.3178578421625376No Hit
AATTCCCCTTGATCAGAAGGGCTGACCTGCTTACTGATTTGTAAAACCGG447290.30871288969662014No Hit
AATTCAGTCATCTGAAAGGGTTAACGTGAAGTACCGTTATGAGCTGACGG443440.3060556770933159No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA441920.30500659575382943No Hit
AATTCCCCTTGATTGCGGGGCTATTTTCAAGTGAAACCGGGTACATTGCC433570.2992435502375721No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA414550.286116229792157No Hit
AATTCCCCTTGATCAGAAGGGGGACGCTGCCGGATTAATCTGTATACCTG400030.276094742259695No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC375790.2593646556352543No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTGGG365270.2521039084698617No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT359180.2479006812609985No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC333650.23028025586817796No Hit
AATTCCCCTTGTTCCGAAGGGAACCCCAGGGAAGCCATCACCCCCACACT282820.19519814765364332No Hit
AATTCCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG241400.16661068115263947No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA239730.165458072049388No Hit
AATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGA221730.1530347403975756No Hit
AATTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC221470.15285529227371608No Hit
AATTCCATGTAAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC213820.14757537632169582No Hit
AATTCGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA202710.13990741995216052No Hit
ATATCTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTT197090.13602857973642798No Hit
AATTCCCCTTGTTCCGAAGGGTAAAACCGGTCCGGCCATCACGCTCACAT185580.1280845493301857No Hit
AATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAA184570.1273874623875007No Hit
AATTCCCCTTGTGCAGAAGGGCCCGTAGAAAAGATCAAAGGATCTTCTTG174870.12069266699735734No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCC164210.11333529391911734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC1462100.093.57841
TATCTCG1503250.090.841942
GTCGACG728600.089.159851
AATTCGA236200.087.703581
CTCGACG526450.086.300055
ATCTCGA1586700.085.9951253
TCTCGAC533350.085.417384
TCTCGAG1076950.084.30034
AATTCCC589000.083.9440461
ATTCCCC575150.083.338772
CTCGAGG1103650.082.176815
TCGAGGG1113250.081.35266
CGAGGGC1113700.081.298427
GAGGGCG1136100.079.675168
AGGGCGC1146250.079.1187069
TCGACTG90350.078.4874654
TCCCCTT599900.074.517394
ATTCGAG92800.074.4742662
ATTCCGA98850.072.845342
TTCCCCT617450.072.565563
Produced by FastQC (version 0.11.5)