##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048255_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14488867
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.287682535839412	31.0	31.0	34.0	27.0	34.0
2	30.58294875644866	31.0	31.0	34.0	27.0	34.0
3	30.649715260689465	31.0	31.0	34.0	27.0	34.0
4	34.14831670412876	37.0	35.0	37.0	30.0	37.0
5	33.91482094493655	37.0	35.0	37.0	28.0	37.0
6	34.055216739859645	37.0	35.0	37.0	30.0	37.0
7	33.946151276010745	37.0	35.0	37.0	29.0	37.0
8	33.93768574174916	37.0	35.0	37.0	29.0	37.0
9	35.28777205284582	39.0	35.0	39.0	28.0	39.0
10-11	35.20199053521576	39.0	35.0	39.0	27.5	39.0
12-13	35.17835649260912	39.0	35.0	39.0	27.0	39.0
14-15	36.30996336704588	40.0	36.0	41.0	27.0	41.0
16-17	36.316382157417834	40.0	36.0	41.0	27.0	41.0
18-19	36.32297401170153	40.0	36.0	41.0	27.0	41.0
20-21	36.25281949237301	40.0	36.0	41.0	27.0	41.0
22-23	36.40694155036415	40.0	36.0	41.0	28.5	41.0
24-25	36.52032622702659	40.0	36.5	41.0	29.0	41.0
26-27	36.57314008748924	40.0	37.0	41.0	30.0	41.0
28-29	36.39783400593021	40.0	36.0	41.0	29.5	41.0
30-31	36.02152090291118	39.5	35.5	41.0	29.0	41.0
32-33	35.66304207913566	38.5	35.0	41.0	29.0	41.0
34-35	35.09618184775938	38.0	35.0	40.0	27.5	41.0
36-37	34.997542181869704	38.0	35.0	40.0	27.0	41.0
38-39	34.80373741438858	38.0	35.0	40.0	26.0	41.0
40-41	34.12282809967128	38.0	33.5	40.0	21.0	41.0
42-43	33.56764089973357	38.0	33.0	40.0	17.5	41.0
44-45	32.855824717005135	37.5	32.5	40.0	10.5	41.0
46-47	32.069015058251274	37.0	30.5	40.0	4.5	41.0
48-49	31.011080334990996	37.0	29.5	40.0	2.0	41.0
50-51	30.69692802756765	37.0	29.0	40.0	2.0	41.0
52-53	30.606557331225417	36.0	29.0	40.0	2.0	41.0
54-55	30.573963374775957	36.0	29.0	40.0	2.0	41.0
56-57	30.265747452854665	36.0	28.5	40.0	2.0	41.0
58-59	30.038638493955393	35.0	28.0	40.0	2.0	41.0
60-61	29.730872883297224	35.0	28.0	39.0	2.0	41.0
62-63	29.374108548308158	35.0	27.0	39.0	2.0	41.0
64-65	29.090765171631432	35.0	26.5	39.0	2.0	41.0
66-67	28.846530650050138	35.0	26.5	38.0	2.0	40.0
68-69	28.491712602510603	35.0	26.0	37.0	2.0	40.0
70-71	28.230592599131455	34.0	26.0	37.0	2.0	40.0
72-73	27.948415048602488	34.0	26.0	36.0	2.0	39.0
74-75	27.68776426755798	34.0	25.5	36.0	2.0	39.0
76-77	27.475960577179706	34.0	25.0	35.5	2.0	38.5
78-79	27.239208524724532	34.0	25.0	35.0	2.0	37.0
80-81	27.025290245262102	34.0	25.0	35.0	2.0	37.0
82-83	26.86911067649389	34.0	25.0	35.0	2.0	36.5
84-85	26.73570096957892	34.0	25.0	35.0	2.0	36.0
86-87	26.84545351268667	34.0	25.0	35.0	2.0	36.0
88-89	26.769085429523233	34.0	25.0	35.0	2.0	36.0
90-91	26.63832175421308	34.0	25.0	35.0	2.0	35.0
92-93	26.46745477061802	34.0	25.0	35.0	2.0	35.0
94-95	26.331393061997186	34.0	24.0	35.0	2.0	35.0
96-97	26.23883717063591	34.0	24.0	35.0	2.0	35.0
98-99	26.063141893703627	34.0	24.0	35.0	2.0	35.0
100-101	25.38158263168542	33.5	20.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7660243230287982
1101	2	0.7798028299990278
1101	3	0.7910020929905599
1101	4	0.8292989083305287
1101	5	0.8378566593467056
1101	6	0.7926384204431187
1101	7	0.8565140425710425
1101	8	0.8314322717401907
1101	9	0.9488060828532738
1101	10-11	0.9433009246037187
1101	12-13	0.9507953815549897
1101	14-15	1.1118904024480116
1101	16-17	1.1409655401231333
1101	18-19	1.1268280311549859
1101	20-21	1.1099114754804233
1101	22-23	1.1199418042937737
1101	24-25	1.0998598447223884
1101	26-27	1.0979993332391587
1101	28-29	1.1125439682811802
1101	30-31	1.060234854730993
1101	32-33	0.9410499379918562
1101	34-35	0.9976719664437965
1101	36-37	1.0064955358281509
1101	38-39	0.9801854477528735
1101	40-41	0.8269768806380213
1101	42-43	0.7939388824282858
1101	44-45	0.7541134445314057
1101	46-47	0.6656679792257094
1101	48-49	0.2860363711757934
1101	50-51	0.14132953556906003
1101	52-53	0.04874160567621999
1101	54-55	0.04341677949454947
1101	56-57	-0.0057148312697954395
1101	58-59	-0.0027167070033371488
1101	60-61	-0.03350095253558649
1101	62-63	-0.0603066357431139
1101	64-65	-0.08873884360253115
1101	66-67	-0.056342618739233075
1101	68-69	-0.10671784361015924
1101	70-71	-0.06628316687354996
1101	72-73	-0.09198212589612353
1101	74-75	-0.15398434061423316
1101	76-77	-0.09095566431534508
1101	78-79	-0.1425487241835377
1101	80-81	-0.1474159929163008
1101	82-83	-0.19107305003529973
1101	84-85	-0.1702861931716555
1101	86-87	-0.12462747774422667
1101	88-89	-0.09146509792274671
1101	90-91	-0.11524348942302609
1101	92-93	-0.1814535455217019
1101	94-95	-0.19741228680886636
1101	96-97	-0.16476044703537696
1101	98-99	-0.17190749159239616
1101	100-101	-0.15575171585831526
1102	1	0.6717064178854102
1102	2	0.669102674938447
1102	3	0.6777020958500835
1102	4	0.7232398497907369
1102	5	0.7520041784814424
1102	6	0.7461119257761695
1102	7	0.7877514448079665
1102	8	0.7606358514442491
1102	9	0.8821669209209801
1102	10-11	0.8751523752946753
1102	12-13	0.8530960906027474
1102	14-15	1.0014786901181694
1102	16-17	1.0248572035449897
1102	18-19	1.0437692664298837
1102	20-21	1.0389649014118802
1102	22-23	1.0316468552808544
1102	24-25	1.0155130985742176
1102	26-27	1.0096789939836341
1102	28-29	0.9721466314572069
1102	30-31	0.9361056227852913
1102	32-33	0.8777610716814834
1102	34-35	0.8956844887277811
1102	36-37	0.89838166979996
1102	38-39	0.8664134577060096
1102	40-41	0.7479373891313301
1102	42-43	0.682551527901964
1102	44-45	0.5754621920792857
1102	46-47	0.4996608742571951
1102	48-49	0.3236739712612007
1102	50-51	0.12780362407557533
1102	52-53	0.060867318308503116
1102	54-55	-0.006873157213121317
1102	56-57	-0.0369867581604062
1102	58-59	-0.007964172646744316
1102	60-61	0.0045526099919932506
1102	62-63	0.026237371620137395
1102	64-65	2.927607831324508E-4
1102	66-67	0.016598313256743324
1102	68-69	0.024704722002883983
1102	70-71	1.880521201123031E-4
1102	72-73	0.02430358563028534
1102	74-75	-0.056791096081912684
1102	76-77	-0.03558010562873548
1102	78-79	-0.04060580914551437
1102	80-81	-0.0698266095955411
1102	82-83	-0.03348969864351048
1102	84-85	-0.031445560282243434
1102	86-87	-0.011266533894392694
1102	88-89	0.018348581726357338
1102	90-91	0.0384540201544894
1102	92-93	0.013167633255413591
1102	94-95	0.02714099705783468
1102	96-97	0.05922339593673698
1102	98-99	0.07336560430581684
1102	100-101	0.07949047462644643
1103	1	0.6076015356897528
1103	2	0.5782777677014934
1103	3	0.5639753099131042
1103	4	0.613278068822531
1103	5	0.6301649742742512
1103	6	0.6366232403266352
1103	7	0.7123540813356257
1103	8	0.6863835119134336
1103	9	0.8266561351007837
1103	10-11	0.7748511687699562
1103	12-13	0.7544007423807813
1103	14-15	0.8863561492975691
1103	16-17	0.8929162960445538
1103	18-19	0.9454038524702213
1103	20-21	0.9393735673770252
1103	22-23	0.8691170613167785
1103	24-25	0.8823858933436739
1103	26-27	0.8725182179231652
1103	28-29	0.8373771142930408
1103	30-31	0.8372922785331554
1103	32-33	0.7585618078232201
1103	34-35	0.811378603874914
1103	36-37	0.8343875684923034
1103	38-39	0.8630402048795673
1103	40-41	0.7166061838411721
1103	42-43	0.6622377865520122
1103	44-45	0.5583566766059249
1103	46-47	0.450870745700513
1103	48-49	0.24703755219683288
1103	50-51	0.1587683307800134
1103	52-53	0.09070437533053166
1103	54-55	0.04950797064412882
1103	56-57	0.00220804530211538
1103	58-59	0.08032518626793461
1103	60-61	0.03855511181374638
1103	62-63	-0.016818533604638475
1103	64-65	0.0655387446022182
1103	66-67	-0.02986996399155828
1103	68-69	-0.020170479137789243
1103	70-71	0.02390894728988613
1103	72-73	0.03392591076363871
1103	74-75	0.015786573509057433
1103	76-77	0.0153023202340421
1103	78-79	0.027973115283121075
1103	80-81	0.03184611573322371
1103	82-83	-0.01716747948698938
1103	84-85	0.002605783791963745
1103	86-87	3.5039733151620567E-4
1103	88-89	0.03339827029608955
1103	90-91	0.031042823583060652
1103	92-93	0.03179081413392382
1103	94-95	0.03779811311696335
1103	96-97	0.0921827467493479
1103	98-99	0.07861641758528393
1103	100-101	0.04611491867922268
1104	1	0.4520076274909748
1104	2	0.398205546196035
1104	3	0.42292524478977
1104	4	0.4595724050453356
1104	5	0.45115805091867855
1104	6	0.4698720856246723
1104	7	0.4654250834907998
1104	8	0.5063986366781208
1104	9	0.6267884594559732
1104	10-11	0.4948546159441989
1104	12-13	0.5214545761355112
1104	14-15	0.5916182198974553
1104	16-17	0.6938945874886642
1104	18-19	0.7029639833280612
1104	20-21	0.6992742772823775
1104	22-23	0.6690065202216289
1104	24-25	0.6487245800533685
1104	26-27	0.6583937957833825
1104	28-29	0.6140032589039564
1104	30-31	0.5890603481562735
1104	32-33	0.5240149141292321
1104	34-35	0.5401211593595434
1104	36-37	0.4945286173764245
1104	38-39	0.5162935416152195
1104	40-41	0.41989321632696175
1104	42-43	0.3643862967682807
1104	44-45	0.30051005483603177
1104	46-47	0.2658095976989543
1104	48-49	0.062321536595110416
1104	50-51	-0.02248148239998926
1104	52-53	-0.07850089145106409
1104	54-55	-0.134580562727443
1104	56-57	-0.10585352352955368
1104	58-59	-0.08834709339122426
1104	60-61	-0.10601736262708172
1104	62-63	-0.05851399877516528
1104	64-65	-0.06841583593539013
1104	66-67	-0.12388926337401074
1104	68-69	-0.08468594399341
1104	70-71	-0.0882779981456494
1104	72-73	-0.04853429683227617
1104	74-75	-0.08556767159789658
1104	76-77	-0.1155870027887218
1104	78-79	-0.10614865465742085
1104	80-81	-0.08966072960055627
1104	82-83	-0.09164468048742336
1104	84-85	-0.1587342361033457
1104	86-87	-0.12992251234935281
1104	88-89	-0.0541068897496082
1104	90-91	-0.1104067551627459
1104	92-93	-0.10169524883774628
1104	94-95	-0.08661038449888991
1104	96-97	-0.09318590744975452
1104	98-99	-0.07388040137702134
1104	100-101	-0.09093024693117258
1105	1	0.3562537778433139
1105	2	0.37843559959550177
1105	3	0.37637129980483053
1105	4	0.43596145354584337
1105	5	0.39827216253836184
1105	6	0.40947835076994465
1105	7	0.4550175263342666
1105	8	0.42109099135549144
1105	9	0.5315243726864765
1105	10-11	0.4165253806355409
1105	12-13	0.4315340627573079
1105	14-15	0.4858688470172865
1105	16-17	0.49984316164803744
1105	18-19	0.5653319507032464
1105	20-21	0.5170029010340969
1105	22-23	0.48056024715957335
1105	24-25	0.5283221378763798
1105	26-27	0.5299651861103101
1105	28-29	0.48684678519414604
1105	30-31	0.49216982701045
1105	32-33	0.43526299023634607
1105	34-35	0.4069963596183612
1105	36-37	0.4341240519048455
1105	38-39	0.45900719692066616
1105	40-41	0.4034864552793209
1105	42-43	0.31869162150967156
1105	44-45	0.2650898078085149
1105	46-47	0.20408913706977216
1105	48-49	0.1012757652711116
1105	50-51	0.004468061862798578
1105	52-53	-0.020904778666732682
1105	54-55	-0.036691768214236475
1105	56-57	9.888755832037077E-4
1105	58-59	-0.04263700504769474
1105	60-61	-0.034733639264597826
1105	62-63	-0.012610849701847116
1105	64-65	-0.038698028411751295
1105	66-67	-0.0454441129322376
1105	68-69	-0.07070544682558833
1105	70-71	-0.054861943738842456
1105	72-73	-0.07112224820300739
1105	74-75	-0.045112782736357104
1105	76-77	-0.024939027879888442
1105	78-79	-0.02267754206561179
1105	80-81	-0.01819721504460503
1105	82-83	0.009544436218440921
1105	84-85	-0.0670244820352508
1105	86-87	-0.020333634632279995
1105	88-89	0.007825861307875215
1105	90-91	-0.023393499192387424
1105	92-93	-0.1425164197304909
1105	94-95	-0.0851811534288025
1105	96-97	-0.042011315606405475
1105	98-99	-0.0030049987047711113
1105	100-101	-0.07384022871332974
1106	1	0.35928475140257277
1106	2	0.33132003845858193
1106	3	0.35656569173501396
1106	4	0.33660359137950735
1106	5	0.34444635937823165
1106	6	0.2826859049158372
1106	7	0.3546410412104706
1106	8	0.3731490989060191
1106	9	0.3978619604241018
1106	10-11	0.3046466703134101
1106	12-13	0.3825179950613702
1106	14-15	0.3959020741467043
1106	16-17	0.42583110066991736
1106	18-19	0.47437022288224995
1106	20-21	0.47147943317038
1106	22-23	0.48852212056269906
1106	24-25	0.47157592654197344
1106	26-27	0.46499469922669334
1106	28-29	0.4689965273186516
1106	30-31	0.45575143477626767
1106	32-33	0.4183297662137093
1106	34-35	0.3982833903053802
1106	36-37	0.42302613419135326
1106	38-39	0.4261140642116459
1106	40-41	0.3270416785322041
1106	42-43	0.31318347699874494
1106	44-45	0.2405659422257429
1106	46-47	0.2573901410728965
1106	48-49	0.15034791417877003
1106	50-51	0.12892485696610478
1106	52-53	0.11418909869355076
1106	54-55	0.12728866267289618
1106	56-57	0.1008333106873387
1106	58-59	0.10201518847947
1106	60-61	0.04149178861717573
1106	62-63	0.02367959972582767
1106	64-65	0.0384569374663144
1106	66-67	0.03935238405893671
1106	68-69	0.022153372665162152
1106	70-71	0.02460917977770194
1106	72-73	0.08200926945338338
1106	74-75	0.032475488941965835
1106	76-77	0.081185445091009
1106	78-79	0.016646335533462064
1106	80-81	-0.030970847169623283
1106	82-83	0.009521877408527502
1106	84-85	0.03974152795419883
1106	86-87	0.05341120740855132
1106	88-89	0.03144070904104268
1106	90-91	0.03233232268544839
1106	92-93	0.007098588733555289
1106	94-95	-0.03168659452850875
1106	96-97	0.04020538402378193
1106	98-99	0.0011323989801361733
1106	100-101	0.014550467485022978
1107	1	0.20106920167363285
1107	2	0.19672922310978436
1107	3	0.16776799822712363
1107	4	0.20791104974784247
1107	5	0.1599019274322231
1107	6	0.15307172613138675
1107	7	0.23116865532157505
1107	8	0.2025001707045746
1107	9	0.25702375581485626
1107	10-11	0.17470249910041957
1107	12-13	0.17260255903061505
1107	14-15	0.1446640721838861
1107	16-17	0.19029765618074634
1107	18-19	0.25582639301634913
1107	20-21	0.26619470029545056
1107	22-23	0.2613177421472699
1107	24-25	0.2598282476062437
1107	26-27	0.20830541246432688
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2216	64-65	-0.9845440537065784
2216	66-67	-1.2343808547794417
2216	68-69	-1.0346191417935735
2216	70-71	-1.23881842859603
2216	72-73	-1.175098154367145
2216	74-75	-0.8936411108288773
2216	76-77	-1.0494362844419172
2216	78-79	-1.0801793407960787
2216	80-81	-1.0495403111347805
2216	82-83	-1.1670224071348585
2216	84-85	-1.0063671694999528
2216	86-87	-0.9546842917502616
2216	88-89	-0.9167491021917016
2216	90-91	-0.816728503618716
2216	92-93	-0.9768523958715996
2216	94-95	-1.029868550536598
2216	96-97	-1.0611733498654274
2216	98-99	-1.0647930702496318
2216	100-101	-1.1912327042654987
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	360315.0
3	61019.0
4	38497.0
5	30637.0
6	26631.0
7	32982.0
8	44048.0
9	49936.0
10	55207.0
11	58841.0
12	66536.0
13	85034.0
14	115634.0
15	174590.0
16	185563.0
17	326751.0
18	542917.0
19	193052.0
20	115147.0
21	93794.0
22	92724.0
23	102898.0
24	123521.0
25	152344.0
26	188327.0
27	229155.0
28	274912.0
29	321364.0
30	360205.0
31	401127.0
32	482209.0
33	580289.0
34	722410.0
35	995846.0
36	1683997.0
37	2500394.0
38	2173649.0
39	446149.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.008541097318	47.57084531469121	10.511856652772408	16.908756935218378
2	19.515727807559795	29.982538478228722	36.790251675324875	13.711482038886608
3	17.207641048594873	31.151838684857825	27.34309879076125	24.297421475786056
4	22.525687936065335	21.308827312080105	37.27063441921512	18.89485033263944
5	18.833862205842383	25.869664537700558	19.866281421450633	35.43019183500643
6	20.665600592652382	20.98418580094454	30.358825586329193	27.991388020073888
7	25.00654284862671	23.577275102406723	19.655961227911796	31.760220821054762
8	33.87015526979141	21.861696374734578	21.558834759562473	22.709313595911535
9	20.086756687811455	30.44158427022903	24.92922267934603	24.542436362613486
10-11	31.803165129176865	21.56946658685835	22.659072664530168	23.96829561943462
12-13	27.84790015407081	22.79973881336343	22.410606253887497	26.941754778678266
14-15	27.086892143281112	22.57654891342661	21.999244166131955	28.337314777160326
16-17	27.765652276654734	26.80635193283122	20.51029772296691	24.917698067547136
18-19	27.14299004616435	23.349801297213297	23.52521835810958	25.981990298512773
20-21	27.135692668851984	28.511798520968156	22.42670856657786	21.92580024360199
22-23	18.905413427521786	25.37727578123307	30.297068145605905	25.420242645639235
24-25	19.32941203753962	24.1693081272448	31.175124127305352	25.326155707910225
26-27	18.530547450258076	23.87224818164486	36.56403605614288	21.03316831195419
28-29	20.794286399739065	22.35975442503115	38.12198232852483	18.72397684670496
30-31	19.402933275066463	22.482933279214556	38.75172703229819	19.362406413420793
32-33	19.150845340986713	22.44176632988732	39.323277933421615	19.084110395704357
34-35	19.08992571924073	22.212104617816195	38.94725686970361	19.75071279323947
36-37	20.10502519625356	22.404599965090206	38.32374304607558	19.166631792580663
38-39	19.22811501403122	22.58725551110311	38.06985693762478	20.114772537240892
40-41	21.035076552895283	23.330863355555532	35.786681010301436	19.847379081247755
42-43	20.785823364283573	23.344857506419427	35.235890931710884	20.633428197586117
44-45	21.2460367416589	25.483938613272727	31.766008494900788	21.504016150167583
46-47	21.80526648471279	25.32846214680406	31.181215025424176	21.685056343058974
48-49	23.59834641361323	25.92295652276225	28.702154084878153	21.776542978746377
50-51	23.807763964466787	26.07115129093249	27.63387395576027	22.48721078884045
52-53	22.75672586126944	26.94957550056313	27.440304896309208	22.85339374185822
54-55	24.003904997522213	25.689435505574632	27.602282778031	22.704376718872155
56-57	22.844899459947598	26.111347842253736	28.16699738919643	22.876755308602238
58-59	22.29321640416178	27.025432931430792	27.669013079270794	23.01233758513664
60-61	23.543997600751577	26.167230034856516	26.79591609199078	23.492856272401124
62-63	23.635359596715205	26.086229872554302	27.728834713432537	22.549575817297956
64-65	21.880472597366435	27.530159861715507	28.14776403480562	22.44160350611244
66-67	21.912733103647223	26.205774311309778	28.55207606836774	23.329416516675263
68-69	23.146374455461046	26.498454889353624	27.613163952130513	22.74200670305481
70-71	22.984648501812107	27.10115232776819	27.372896728450343	22.54130244196936
72-73	23.321818183012514	26.872178235871502	27.283943305784717	22.522060275331267
74-75	22.83675686498765	26.83103269157628	27.151396131509138	23.180814311926937
76-77	22.94183689211692	27.444888317542514	26.9244578931844	22.688816897156165
78-79	23.269891191029775	26.908064852940704	26.979557688119023	22.8424862679105
80-81	22.230999545775234	26.80701074719784	27.62549328806087	23.336496418966057
82-83	22.482834904348252	28.05491309608053	26.078162072766947	23.384089926804272
84-85	23.03341440458496	26.54542973506682	26.306882067545384	24.11427379280284
86-87	22.800538907481904	26.638626795162967	27.081604459466323	23.479229837888806
88-89	22.616827817640942	28.118776210873627	26.310447340883634	22.953948630601793
90-91	23.41843104454662	27.117537789084267	25.720399061994474	23.743632104374633
92-93	24.90624311068591	26.674470148880737	25.827963772983654	22.591322967449702
94-95	23.466839019497137	27.880749513563728	26.4806938971417	22.171717569797433
96-97	22.356895892412922	28.83165687135545	26.236318258161894	22.575128978069735
98-99	24.681820878280714	27.266017229427614	25.700485653643952	22.35167623864772
100-101	24.01244254603428	27.578491058596178	25.686424491500198	22.72264190386935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2247.0
1	2680.5
2	3261.5
3	3538.5
4	3550.5
5	3353.5
6	3106.5
7	2731.0
8	2387.0
9	2110.5
10	1824.5
11	1577.0
12	1481.5
13	1473.0
14	1482.5
15	1596.5
16	2281.5
17	2818.5
18	3234.0
19	4488.0
20	6469.0
21	9193.5
22	13137.5
23	18899.0
24	25800.5
25	34768.0
26	47538.0
27	66081.5
28	89203.0
29	119303.5
30	159119.5
31	200093.0
32	241076.5
33	293425.5
34	364899.5
35	430225.5
36	460063.0
37	470712.5
38	474834.5
39	480440.5
40	480925.0
41	469803.0
42	449579.0
43	452841.0
44	469414.5
45	464723.0
46	464389.0
47	470424.0
48	509537.0
49	550533.5
50	508685.0
51	486272.0
52	535254.0
53	570067.0
54	515275.0
55	484304.5
56	481378.0
57	419407.5
58	349861.0
59	279853.0
60	233366.5
61	189033.0
62	148740.5
63	114592.5
64	83579.5
65	59732.5
66	42764.0
67	30356.0
68	22896.0
69	18685.5
70	15633.0
71	12787.5
72	11207.0
73	9455.5
74	7319.0
75	5983.0
76	4808.5
77	3593.5
78	2476.0
79	1485.5
80	742.0
81	456.5
82	330.5
83	207.0
84	121.0
85	85.0
86	69.0
87	50.5
88	35.5
89	34.0
90	29.5
91	29.0
92	25.0
93	16.0
94	16.0
95	13.0
96	12.0
97	15.5
98	16.0
99	21.5
100	75.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5259141380758068
2	0.117655852593581
3	0.14211601224581605
4	0.13006538054355804
5	0.12958915283023856
6	0.12649022176820313
7	0.13023792681649987
8	0.13038976753668868
9	0.13731922585803294
10-11	0.15555391598252644
12-13	0.15058113239634266
14-15	0.1385822645759672
16-17	0.1257275672418002
18-19	0.14339285466558566
20-21	0.15630621773255288
22-23	0.1351209863407539
24-25	0.15160260633215833
26-27	0.1766149140578073
28-29	0.14398296291904675
30-31	0.168311987403846
32-33	0.1462122607654553
34-35	0.41942548026702153
36-37	0.1390619432147455
38-39	0.14216087427678092
40-41	0.14580505156131254
42-43	0.2528631120708058
44-45	0.14257843625730018
46-47	0.17848876658195567
48-49	0.27795134015654915
50-51	0.13285372831429815
52-53	0.16122033558593643
54-55	0.17495846983756563
56-57	0.1852767369594876
58-59	0.17852672676200285
60-61	0.1794791821886418
62-63	0.2070762330829595
64-65	0.16298720942086087
66-67	0.15900484144136323
68-69	0.1592567589998583
70-71	0.17952749514506552
72-73	0.18369621309934034
74-75	0.17457541711163474
76-77	0.16838790776394041
78-79	0.1689607613901073
80-81	0.16261796039676532
82-83	0.17049987414474851
84-85	0.15726212408465065
86-87	0.1557195604045506
88-89	0.15147147116472254
90-91	0.1739231921999146
92-93	0.1624005520928586
94-95	0.17608692246260527
96-97	0.17236682481797921
98-99	0.1758108484258983
100-101	0.14554968307735863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4488867E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.783292236013544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91887358697392	27.881938242636846
2	11.343178916228558	8.117925720895878
3	3.834621162887574	4.116461090580245
4	1.7939397700561244	2.56772284182901
5	0.9943509426373833	1.7790575182774513
6	0.6443020773719762	1.383314971372322
7	0.4648778294532873	1.1644401457759452
8	0.34476203172397346	0.9869376426448572
9	0.2728076491596738	0.8785760250690419
>10	1.8747454926440688	13.109275673134396
>50	0.2663082422562443	6.767499094558792
>100	0.23211979669618735	15.665573136784957
>500	0.011450773868839456	2.6608284962450366
>1k	0.0023624108110664567	1.6544545990933062
>5k	5.118522426229795E-4	1.2222387347496444
>10k+	7.874649886507376E-4	10.043756066352229
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	167426	1.1555492917424117	No Hit
AATTCCCCTTCAGTAGAAGGGCACACCGGTATCCCCGCCCGACAACGACG	74457	0.5138911137772194	No Hit
AATTCCCCTTGGTTGCAAGGGAACGATGCGTAATGTGTGTATTGCCGTTG	73239	0.505484659359493	No Hit
AATTCGACTGTCACCGAAGTGCGGCAAAGACAGCAACGGCAATACACACA	70749	0.48829905057448597	No Hit
AATTCCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGG	60176	0.4153257808219235	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	59814	0.4128273107897256	No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT	58352	0.4027368047480869	No Hit
AATTCCCCTTCTACTGAAGGGACAGGTGTTCTCCCGTATTGTTGACATGC	50209	0.34653503272547126	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	46054	0.3178578421625376	No Hit
AATTCCCCTTGATCAGAAGGGCTGACCTGCTTACTGATTTGTAAAACCGG	44729	0.30871288969662014	No Hit
AATTCAGTCATCTGAAAGGGTTAACGTGAAGTACCGTTATGAGCTGACGG	44344	0.3060556770933159	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	44192	0.30500659575382943	No Hit
AATTCCCCTTGATTGCGGGGCTATTTTCAAGTGAAACCGGGTACATTGCC	43357	0.2992435502375721	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	41455	0.286116229792157	No Hit
AATTCCCCTTGATCAGAAGGGGGACGCTGCCGGATTAATCTGTATACCTG	40003	0.276094742259695	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	37579	0.2593646556352543	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTGGG	36527	0.2521039084698617	No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT	35918	0.2479006812609985	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	33365	0.23028025586817796	No Hit
AATTCCCCTTGTTCCGAAGGGAACCCCAGGGAAGCCATCACCCCCACACT	28282	0.19519814765364332	No Hit
AATTCCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG	24140	0.16661068115263947	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTAAA	23973	0.165458072049388	No Hit
AATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGA	22173	0.1530347403975756	No Hit
AATTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC	22147	0.15285529227371608	No Hit
AATTCCATGTAAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC	21382	0.14757537632169582	No Hit
AATTCGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA	20271	0.13990741995216052	No Hit
ATATCTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTT	19709	0.13602857973642798	No Hit
AATTCCCCTTGTTCCGAAGGGTAAAACCGGTCCGGCCATCACGCTCACAT	18558	0.1280845493301857	No Hit
AATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAA	18457	0.1273874623875007	No Hit
AATTCCCCTTGTGCAGAAGGGCCCGTAGAAAAGATCAAAGGATCTTCTTG	17487	0.12069266699735734	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCC	16421	0.11333529391911734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1411105506041295E-5	0.0	6.901850917673549E-6	0.0	6.901850917673549E-6
2	5.5214807341388394E-5	0.0	6.901850917673549E-6	0.0	6.901850917673549E-6
3	7.592036009440903E-5	0.0	6.901850917673549E-6	6.901850917673549E-6	6.901850917673549E-6
4	1.0352776376510323E-4	0.0	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5
5	1.3113516743579743E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	1.3803701835347098E-5
6	2.001536766125329E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	2.0705552753020648E-5
7	2.4156478211857422E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	2.0705552753020648E-5
8	2.7607403670694197E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	2.7607403670694197E-5
9	3.450925458836774E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	3.4509254588367746E-5
10-11	5.279915952020265E-4	6.901850917673549E-6	6.901850917673549E-6	1.3803701835347098E-5	3.4509254588367746E-5
12-13	7.453998991087433E-4	1.0352776376510324E-5	1.0352776376510324E-5	1.3803701835347098E-5	3.4509254588367746E-5
14-15	9.490045011801131E-4	1.3803701835347098E-5	2.0705552753020648E-5	1.3803701835347098E-5	3.4509254588367746E-5
16-17	0.0011664128050868296	1.3803701835347098E-5	4.1411105506041295E-5	1.3803701835347098E-5	3.796018004720452E-5
18-19	0.0013838211089935465	1.3803701835347098E-5	6.211665825906194E-5	1.3803701835347098E-5	4.4862030964878073E-5
20-21	0.001635738667488631	1.3803701835347098E-5	8.282221101208259E-5	1.3803701835347098E-5	5.5214807341388394E-5
22-23	0.001956674735160451	2.0705552753020648E-5	2.6227033487159486E-4	2.0705552753020648E-5	6.901850917673549E-5
24-25	0.002491568181280151	2.4156478211857422E-5	4.1411105506041293E-4	2.0705552753020648E-5	6.901850917673549E-5
26-27	0.0036165698808609398	3.1058329129530975E-5	5.383443715785369E-4	2.7607403670694197E-5	7.937128555324581E-5
28-29	0.005103918753619589	3.796018004720452E-5	6.694795390143342E-4	2.7607403670694197E-5	8.282221101208259E-5
30-31	0.0076023887858174145	4.1411105506041295E-5	7.35047122732233E-4	2.7607403670694197E-5	8.282221101208259E-5
32-33	0.011029157766442331	4.1411105506041295E-5	7.488508245675801E-4	2.7607403670694197E-5	8.972406192975613E-5
34-35	0.016043352458132164	4.1411105506041295E-5	7.523017500264169E-4	3.1058329129530975E-5	9.317498738859291E-5
36-37	0.022762304326487362	4.4862030964878073E-5	7.523017500264169E-4	3.4509254588367746E-5	9.662591284742969E-5
38-39	0.031589771650191834	4.8312956423714845E-5	7.592036009440903E-4	3.796018004720452E-5	1.0352776376510323E-4
40-41	0.04366455983066171	4.8312956423714845E-5	7.592036009440903E-4	4.1411105506041295E-5	1.1042961468277679E-4
42-43	0.0585656559619189	4.8312956423714845E-5	7.592036009440903E-4	4.4862030964878073E-5	1.1042961468277679E-4
44-45	0.07666575999351778	4.8312956423714845E-5	7.592036009440903E-4	5.1763881882551616E-5	1.1042961468277679E-4
46-47	0.09931418377986354	4.8312956423714845E-5	7.592036009440903E-4	5.5214807341388394E-5	1.3113516743579743E-4
48-49	0.12650057454457964	7.246943463557227E-5	7.592036009440903E-4	5.5214807341388394E-5	1.3803701835347098E-4
50-51	0.15748298331401622	7.592036009440903E-5	7.592036009440903E-4	5.5214807341388394E-5	1.4493886927114454E-4
52-53	0.1936210747189549	7.937128555324581E-5	7.592036009440903E-4	5.5214807341388394E-5	1.4493886927114454E-4
54-55	0.23641600133398974	8.627313647091936E-5	7.592036009440903E-4	5.5214807341388394E-5	1.4493886927114454E-4
56-57	0.2830690626120041	9.317498738859291E-5	7.592036009440903E-4	5.5214807341388394E-5	1.4493886927114454E-4
58-59	0.3369794201299522	1.0007683830626647E-4	7.592036009440903E-4	5.5214807341388394E-5	1.5184072018881807E-4
60-61	0.39615934082354404	1.1042961468277679E-4	7.592036009440903E-4	5.5214807341388394E-5	1.5874257110649163E-4
62-63	0.4610194848223812	1.1733146560045033E-4	7.592036009440903E-4	5.5214807341388394E-5	1.5874257110649163E-4
64-65	0.533689073134566	1.2768424197696064E-4	7.592036009440903E-4	5.5214807341388394E-5	1.6909534748300195E-4
66-67	0.6128360485329875	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	1.7944812385951227E-4
68-69	0.7008380986587841	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	1.8980090023602261E-4
70-71	0.7968083356690348	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	1.9670275115369614E-4
72-73	0.900167004086655	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	2.001536766125329E-4
74-75	1.011883813965578	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	2.1740830390671678E-4
76-77	1.1353234176281695	1.3113516743579743E-4	7.592036009440903E-4	5.5214807341388394E-5	2.2431015482439034E-4
78-79	1.2672005340376167	1.3458609289463422E-4	7.592036009440903E-4	5.5214807341388394E-5	2.277610802832271E-4
80-81	1.4089265917065843	1.3803701835347098E-4	7.592036009440903E-4	5.5214807341388394E-5	2.277610802832271E-4
82-83	1.560225516598365	1.3803701835347098E-4	7.592036009440903E-4	5.5214807341388394E-5	2.312120057420639E-4
84-85	1.721155974445759	1.483897947299813E-4	7.592036009440903E-4	5.8665732800225165E-5	2.3811385665973743E-4
86-87	1.8944372945103298	1.5184072018881807E-4	7.592036009440903E-4	6.211665825906194E-5	2.4846663303624775E-4
88-89	2.0780472344731993	1.5184072018881807E-4	7.592036009440903E-4	6.211665825906194E-5	2.5191755849508454E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	146210	0.0	93.5784	1
TATCTCG	150325	0.0	90.84194	2
GTCGACG	72860	0.0	89.15985	1
AATTCGA	23620	0.0	87.70358	1
CTCGACG	52645	0.0	86.30005	5
ATCTCGA	158670	0.0	85.995125	3
TCTCGAC	53335	0.0	85.41738	4
TCTCGAG	107695	0.0	84.3003	4
AATTCCC	58900	0.0	83.944046	1
ATTCCCC	57515	0.0	83.33877	2
CTCGAGG	110365	0.0	82.17681	5
TCGAGGG	111325	0.0	81.3526	6
CGAGGGC	111370	0.0	81.29842	7
GAGGGCG	113610	0.0	79.67516	8
AGGGCGC	114625	0.0	79.118706	9
TCGACTG	9035	0.0	78.487465	4
TCCCCTT	59990	0.0	74.51739	4
ATTCGAG	9280	0.0	74.474266	2
ATTCCGA	9885	0.0	72.84534	2
TTCCCCT	61745	0.0	72.56556	3
>>END_MODULE