FastQCFastQC Report
Tue 31 May 2016
SRR2048255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488867
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT904270.624113672932466No Hit
AATTCGACTGTCACCGAAGTGCGGCAAAGACAGCAACGGCAATACACACA806190.5564203191319238No Hit
AATTCCCCTTCAGTAGAAGGGCACACCGGTATCCCCGCCCGACAACGACG777170.5363911477688352No Hit
AATTCCCCTTGGTTGCAAGGGAACGATGCGTAATGTGTGTATTGCCGTTG770960.5321050983489599No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT719770.496774523501389No Hit
AATTCCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGG693270.4784846185695541No Hit
AATTCCCCTTGATCAGAAGGGGGACGCTGCCGGATTAATCTGTATACCTG548140.3783180562013579No Hit
AATTCCCCTTCTACTGAAGGGACAGGTGTTCTCCCGTATTGTTGACATGC523800.3615189510677405No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT522680.36074594376496105No Hit
AATTCCCCTTGATTGCGGGGCTATTTTCAAGTGAAACCGGGTACATTGCC477680.32968761463543006No Hit
AATTCCCCTTGATCAGAAGGGCTGACCTGCTTACTGATTTGTAAAACCGG473910.32708561683946713No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT416960.2877795758633163No Hit
AATTCAGTCATCTGAAAGGGTTAACGTGAAGTACCGTTATGAGCTGACGG405970.2801944417047931No Hit
AATTCCCCTTGTTCCGAAGGGAACCCCAGGGAAGCCATCACCCCCACACT313760.21655247439292527No Hit
AATTCCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG280470.19357621268799002No Hit
AATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGA249880.17246345073082664No Hit
AATTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC236350.16312524643921433No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC234050.1615378207281494TruSeq Adapter, Index 12 (100% over 50bp)
AATTCCATGTAAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC230070.15879088406291533No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT222450.1535316736636481No Hit
AATTCGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA216130.14916970388367842No Hit
AATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAA213890.14762368927811956No Hit
AATTCCCCTTGTTCCGAAGGGTAAAACCGGTCCGGCCATCACGCTCACAT199380.13760910359657522No Hit
AATTCCCCTTGTGCAGAAGGGCCCGTAGAAAAGATCAAAGGATCTTCTTG182200.12575172372001206No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG166020.11458452893521626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC326850.087.7833251
AATTCGA242000.087.123541
AATTCCC600550.084.87121
ATTCCCC589200.084.241412
TCGACTG94700.078.706354
TCCCCTT598900.077.535264
TTCCCCT616300.075.35473
ATTCGAA110200.073.0345842
TCGACGT14250.072.001697
CCCCTTG459500.071.706815
TTCGACT104600.071.166993
TTCGAAC99750.071.055423
ATTCCGA99250.070.94382
TCGACGG193600.070.898812
AATTCCG100950.070.324831
GTCGACG196650.069.276491
CACTACG37800.068.734128
ATTCGAG80550.067.831132
ATTCGAC113300.065.9203342
TCGAACC108450.065.61744
Produced by FastQC (version 0.11.5)