##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048255_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14488867
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14510057963815	33.0	31.0	34.0	30.0	34.0
2	32.274372661437226	34.0	31.0	34.0	30.0	34.0
3	32.312055041985	34.0	31.0	34.0	30.0	34.0
4	35.89853954764027	37.0	35.0	37.0	35.0	37.0
5	35.6480466001931	37.0	35.0	37.0	33.0	37.0
6	35.57269771335467	37.0	35.0	37.0	33.0	37.0
7	35.46405105381946	37.0	35.0	37.0	32.0	37.0
8	35.40045871081569	37.0	35.0	37.0	32.0	37.0
9	36.8383264198643	39.0	37.0	39.0	32.0	39.0
10-11	36.867235788692106	39.0	37.0	39.0	32.0	39.0
12-13	36.88950271266897	39.0	37.0	39.0	32.0	39.0
14-15	38.25389745795859	40.0	38.0	41.0	32.5	41.0
16-17	38.40551131430773	40.0	38.0	41.0	33.0	41.0
18-19	38.534926298930074	40.0	38.0	41.0	33.5	41.0
20-21	38.57202015865008	40.0	38.0	41.0	34.0	41.0
22-23	38.69584975139878	40.0	38.0	41.0	34.0	41.0
24-25	38.69921129788823	40.0	38.0	41.0	34.0	41.0
26-27	38.58705687615188	40.0	38.0	41.0	34.0	41.0
28-29	38.35410877882998	40.0	37.5	41.0	34.0	41.0
30-31	38.05095350105705	40.0	37.0	41.0	34.0	41.0
32-33	37.89043711975546	40.0	36.5	41.0	33.0	41.0
34-35	37.78871674369017	40.0	36.0	41.0	33.0	41.0
36-37	37.61731131219577	40.0	36.0	41.0	33.0	41.0
38-39	37.471591567511794	40.0	36.0	41.0	33.0	41.0
40-41	37.130718157603354	40.0	36.0	41.0	32.0	41.0
42-43	36.77393832795897	40.0	35.0	41.0	31.0	41.0
44-45	36.363336829580945	39.5	35.0	41.0	30.5	41.0
46-47	35.72338389192198	39.0	35.0	41.0	28.0	41.0
48-49	35.347725084369955	39.0	35.0	41.0	26.5	41.0
50-51	35.12328866018302	39.0	35.0	41.0	24.5	41.0
52-53	34.948016018091685	39.0	35.0	41.0	24.0	41.0
54-55	34.72943508971406	38.5	35.0	40.5	24.0	41.0
56-57	34.441074999170056	38.0	34.5	40.0	23.0	41.0
58-59	34.2546876853794	38.0	34.0	40.0	23.5	41.0
60-61	33.96192110811701	37.0	34.0	40.0	23.0	41.0
62-63	33.61692698262742	36.5	34.0	40.0	22.0	41.0
64-65	33.30205105064461	36.0	33.0	39.5	22.0	41.0
66-67	33.05540271023262	35.5	33.0	39.0	21.5	41.0
68-69	32.73474696123582	35.0	33.0	39.0	20.0	41.0
70-71	32.447392573898284	35.0	33.0	38.0	20.0	40.0
72-73	32.10985793437126	35.0	33.0	37.0	19.0	40.0
74-75	31.892260312693878	35.0	33.0	37.0	19.0	39.0
76-77	31.751124259750608	35.0	33.0	36.0	19.0	39.0
78-79	31.526379460864675	35.0	33.0	36.0	18.5	38.5
80-81	31.293654603910714	35.0	33.0	36.0	18.0	37.0
82-83	31.107339897591718	35.0	33.0	35.0	17.5	37.0
84-85	30.89670451802753	35.0	32.0	35.0	16.0	36.5
86-87	30.715214481574023	35.0	32.0	35.0	12.5	36.0
88-89	30.58279232599761	35.0	32.0	35.0	8.5	36.0
90-91	30.43552025151449	35.0	32.0	35.0	6.0	36.0
92-93	30.241687013898325	35.0	32.0	35.0	2.0	35.0
94-95	30.10736246664422	35.0	32.0	35.0	2.0	35.0
96-97	30.042395033372863	35.0	32.0	35.0	2.0	35.0
98-99	29.89960032761706	35.0	32.0	35.0	2.0	35.0
100-101	29.32905878699832	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34314454585410203
1101	2	0.3616477961351521
1101	3	0.32897447663141577
1101	4	0.21359981709012033
1101	5	0.2520324214581322
1101	6	0.17804808799720462
1101	7	0.19960088375292173
1101	8	0.2199074708322044
1101	9	0.25596214653830884
1101	10-11	0.2171851392253501
1101	12-13	0.1848019415156017
1101	14-15	0.32531266870946496
1101	16-17	0.40568734780443805
1101	18-19	0.4170793965848105
1101	20-21	0.48037988923406516
1101	22-23	0.4610963843312632
1101	24-25	0.4347040644750635
1101	26-27	0.41329556783281873
1101	28-29	0.4422851879423533
1101	30-31	0.4601831413039008
1101	32-33	0.4627904460556209
1101	34-35	0.46197789008202506
1101	36-37	0.5147495053673339
1101	38-39	0.4887742447996857
1101	40-41	0.4520204997802679
1101	42-43	0.48498839833130347
1101	44-45	0.4292507415864648
1101	46-47	0.3765521813988286
1101	48-49	0.3021538817044629
1101	50-51	0.27267292283709565
1101	52-53	0.2787266742676593
1101	54-55	0.2736718949705548
1101	56-57	0.2540820017782863
1101	58-59	0.24740167430700666
1101	60-61	0.2657778043541512
1101	62-63	0.1971695784597216
1101	64-65	0.22640824640611612
1101	66-67	0.1955560211162748
1101	68-69	0.19767299486836976
1101	70-71	0.2078692372839015
1101	72-73	0.18857957955947313
1101	74-75	0.22625783109977604
1101	76-77	0.2255973917870513
1101	78-79	0.2232673643441352
1101	80-81	0.22192207905050765
1101	82-83	0.23334235754462185
1101	84-85	0.22909602502119952
1101	86-87	0.2202890033852114
1101	88-89	0.25476165190681854
1101	90-91	0.26440112549606454
1101	92-93	0.309791175587911
1101	94-95	0.29793083236545215
1101	96-97	0.32111341700287
1101	98-99	0.35673278469973013
1101	100-101	0.46229046892367265
1102	1	0.33539713733672016
1102	2	0.33535681041676924
1102	3	0.32457904506417634
1102	4	0.21207920819971804
1102	5	0.24718174006747518
1102	6	0.198457557451313
1102	7	0.19532155934958695
1102	8	0.2265717127036595
1102	9	0.2099299991929584
1102	10-11	0.19873974307624565
1102	12-13	0.18985191726488893
1102	14-15	0.31172270102761246
1102	16-17	0.3979082173073891
1102	18-19	0.4038254816471607
1102	20-21	0.4542269369948002
1102	22-23	0.4325141176620164
1102	24-25	0.39892751174762253
1102	26-27	0.40089196766651014
1102	28-29	0.4347374052158628
1102	30-31	0.443296048710323
1102	32-33	0.4868951365935459
1102	34-35	0.5177812518583238
1102	36-37	0.5410607199614077
1102	38-39	0.4870034241194716
1102	40-41	0.4697018041801613
1102	42-43	0.4752516194816252
1102	44-45	0.41263079259794466
1102	46-47	0.4406732257709578
1102	48-49	0.4145218084820925
1102	50-51	0.3347668274093891
1102	52-53	0.3563488647785604
1102	54-55	0.3368278673033913
1102	56-57	0.3654254852612979
1102	58-59	0.34758941502924756
1102	60-61	0.32566341377916785
1102	62-63	0.29314568291215437
1102	64-65	0.2829714937340668
1102	66-67	0.25677349911897807
1102	68-69	0.2304467023051302
1102	70-71	0.2383880361583124
1102	72-73	0.22469088461983233
1102	74-75	0.30018890890587
1102	76-77	0.31592329897560134
1102	78-79	0.28260420826316945
1102	80-81	0.22985255785724235
1102	82-83	0.25643724304591586
1102	84-85	0.2513842976259255
1102	86-87	0.2479412515795083
1102	88-89	0.2589570031146238
1102	90-91	0.25601028038045115
1102	92-93	0.2643861176352331
1102	94-95	0.2655762762555298
1102	96-97	0.2672181740205879
1102	98-99	0.3429201313674284
1102	100-101	0.47199777101704754
1103	1	0.2619176957309861
1103	2	0.2926430145175303
1103	3	0.23942327840769906
1103	4	0.17372927965925555
1103	5	0.17211913996003148
1103	6	0.19190938826327653
1103	7	0.18173341505830365
1103	8	0.18371680056510797
1103	9	0.1875634654807996
1103	10-11	0.17640109606185206
1103	12-13	0.1768190672201797
1103	14-15	0.3116915528127677
1103	16-17	0.3572530178619431
1103	18-19	0.35896868565991724
1103	20-21	0.3971950889614888
1103	22-23	0.38274534837702134
1103	24-25	0.3845502648117005
1103	26-27	0.38369989173121866
1103	28-29	0.37798714898607244
1103	30-31	0.32717936573092743
1103	32-33	0.42468747563811604
1103	34-35	0.41546881634960187
1103	36-37	0.4750013292136046
1103	38-39	0.42851949098990616
1103	40-41	0.3848407134111369
1103	42-43	0.3935571051167557
1103	44-45	0.3696838401568172
1103	46-47	0.39648680180212637
1103	48-49	0.37199506064303023
1103	50-51	0.32906898055897926
1103	52-53	0.326214281815254
1103	54-55	0.342336197314161
1103	56-57	0.3527073397483207
1103	58-59	0.3774649606362175
1103	60-61	0.37502110333039695
1103	62-63	0.336591834189619
1103	64-65	0.30555313886501523
1103	66-67	0.3157525399326815
1103	68-69	0.29257505931074945
1103	70-71	0.3212523248733419
1103	72-73	0.3009099983326564
1103	74-75	0.3025502282606176
1103	76-77	0.31074015239201813
1103	78-79	0.28809357742149544
1103	80-81	0.29336304114461953
1103	82-83	0.32710921726642184
1103	84-85	0.3454514200632701
1103	86-87	0.3752453574173735
1103	88-89	0.37291198902674694
1103	90-91	0.33989994109090205
1103	92-93	0.3507114830991256
1103	94-95	0.3582585229868869
1103	96-97	0.35412156036666076
1103	98-99	0.4198728974621879
1103	100-101	0.49903683772574325
1104	1	0.18106772533636217
1104	2	0.19390027943433807
1104	3	0.18797069333623995
1104	4	0.11405520415748782
1104	5	0.13708446606081282
1104	6	0.15331563122806813
1104	7	0.1374422099960242
1104	8	0.10913701081998539
1104	9	0.1293797893458617
1104	10-11	0.12691701893714935
1104	12-13	0.13269027122264276
1104	14-15	0.24042550932278317
1104	16-17	0.2403602401152014
1104	18-19	0.27053039969815984
1104	20-21	0.2724947566829101
1104	22-23	0.2579510909041218
1104	24-25	0.2516381078772909
1104	26-27	0.22888799030307894
1104	28-29	0.20530880290004916
1104	30-31	0.23278756618744012
1104	32-33	0.2692105687086581
1104	34-35	0.32412864298940036
1104	36-37	0.28623619922110777
1104	38-39	0.3081465533607428
1104	40-41	0.18723554517991658
1104	42-43	0.25967151985473436
1104	44-45	0.21844944436926284
1104	46-47	0.28406351783313255
1104	48-49	0.2273971730489066
1104	50-51	0.16758241382721195
1104	52-53	0.15575761307380276
1104	54-55	0.166437828639026
1104	56-57	0.07890775970471964
1104	58-59	0.11966819111943039
1104	60-61	0.15151102997249666
1104	62-63	0.15068796522182026
1104	64-65	0.13284359272943647
1104	66-67	0.12593072333334732
1104	68-69	0.15365678340477018
1104	70-71	0.11218052842792048
1104	72-73	0.12899325265063766
1104	74-75	0.12480457200073047
1104	76-77	0.14430708629085487
1104	78-79	0.14479008340990163
1104	80-81	0.12367593209193473
1104	82-83	0.12903624548622972
1104	84-85	0.13112543169140167
1104	86-87	0.14461184446337683
1104	88-89	0.16024371727593945
1104	90-91	0.1828893332950834
1104	92-93	0.21560392984536492
1104	94-95	0.20178123819401605
1104	96-97	0.1913363506220911
1104	98-99	0.2264963273350773
1104	100-101	0.2631866777861589
1105	1	0.1922866304054125
1105	2	0.20323426474963213
1105	3	0.20291495084022415
1105	4	0.1070373779558551
1105	5	0.13853559360640588
1105	6	0.10443739599137558
1105	7	0.1203852448333933
1105	8	0.09624395421061394
1105	9	0.14359539434166635
1105	10-11	0.12707289141955158
1105	12-13	0.14791561946964293
1105	14-15	0.20738420881665576
1105	16-17	0.24946230444322026
1105	18-19	0.19496565428748625
1105	20-21	0.23648208219230327
1105	22-23	0.22204053245253164
1105	24-25	0.22683216179163423
1105	26-27	0.17991316351751152
1105	28-29	0.18874702594552417
1105	30-31	0.1997097588967165
1105	32-33	0.2048242277463288
1105	34-35	0.23236566309059015
1105	36-37	0.20326721394304315
1105	38-39	0.24261317338272903
1105	40-41	0.19348683228485442
1105	42-43	0.2474340969098705
1105	44-45	0.18439617267167563
1105	46-47	0.231916249672949
1105	48-49	0.1612132418951333
1105	50-51	0.10947708904785003
1105	52-53	0.14740439196269506
1105	54-55	0.154633249550848
1105	56-57	0.15905208170926244
1105	58-59	0.10822672620987817
1105	60-61	0.10383630444218284
1105	62-63	0.08047890340809971
1105	64-65	0.08198343689049636
1105	66-67	0.04776138809987884
1105	68-69	0.038197218716874204
1105	70-71	0.043491060072490484
1105	72-73	0.03381536564986476
1105	74-75	0.09875828029655409
1105	76-77	0.09229774366805188
1105	78-79	0.10159069703866308
1105	80-81	0.06790384519188208
1105	82-83	0.04669967139294684
1105	84-85	0.05363678706625308
1105	86-87	0.06652164225057788
1105	88-89	0.04207598569531257
1105	90-91	0.036117220001536054
1105	92-93	0.07032353438427918
1105	94-95	0.05422437197007923
1105	96-97	0.03305819907240348
1105	98-99	0.0672027329313103
1105	100-101	0.14800621958196913
1106	1	0.13384104178761902
1106	2	0.15188489821159834
1106	3	0.12832547059921495
1106	4	0.09112663340471983
1106	5	0.08230152836559057
1106	6	0.07815718866395116
1106	7	0.08202051976664393
1106	8	0.0810281730059117
1106	9	0.13660847952980504
1106	10-11	0.10963980976954701
1106	12-13	0.1177314285583293
1106	14-15	0.19622808906547817
1106	16-17	0.19232444382256375
1106	18-19	0.19508338323719698
1106	20-21	0.22247720018442863
1106	22-23	0.1846833281017055
1106	24-25	0.1781967233031665
1106	26-27	0.174466815664573
1106	28-29	0.1315991311990885
1106	30-31	0.1437468615403361
1106	32-33	0.12723668012723977
1106	34-35	0.18418282837816236
1106	36-37	0.13086670853942906
1106	38-39	0.20869101899327092
1106	40-41	0.14008548702388168
1106	42-43	0.2150860267459649
1106	44-45	0.19778708839862702
1106	46-47	0.2220515229372637
1106	48-49	0.11167466532882742
1106	50-51	0.08408954918286327
1106	52-53	0.07348983249239183
1106	54-55	0.10030923201834696
1106	56-57	0.06985723814072031
1106	58-59	0.04134569864145021
1106	60-61	0.06038219785809673
1106	62-63	0.04138186175492109
1106	64-65	0.027304003844278668
1106	66-67	0.03223100059211248
1106	68-69	-0.006412850995658914
1106	70-71	0.024001439666960778
1106	72-73	0.07845502422257056
1106	74-75	0.07138810193405831
1106	76-77	0.05215787185734655
1106	78-79	0.04903103725407121
1106	80-81	0.030738347076365358
1106	82-83	-0.011354298749626679
1106	84-85	0.0358391903780273
1106	86-87	0.02955251824156946
1106	88-89	0.058335254359544564
1106	90-91	0.06323398276866499
1106	92-93	0.09006440334265164
1106	94-95	0.10615102425655465
1106	96-97	0.07346993201573682
1106	98-99	0.10549474396473713
1106	100-101	0.13081248315647542
1107	1	0.05964912471304018
1107	2	0.08969622590429793
1107	3	0.10809580128362484
1107	4	0.08093654883570167
1107	5	-0.05022209140869904
1107	6	0.012743367187773913
1107	7	0.029614386429130946
1107	8	0.03987856367609055
1107	9	0.06029123988525242
1107	10-11	0.04033178265775916
1107	12-13	0.058121623946462364
1107	14-15	0.15412452118523134
1107	16-17	0.12544709794523357
1107	18-19	0.11954188239089802
1107	20-21	0.17397059624454414
1107	22-23	0.1556288363164171
1107	24-25	0.13237679071391284
1107	26-27	0.13136986611959145
1107	28-29	0.10009099124778942
1107	30-31	0.08221452726262157
1107	32-33	0.06117176861836526
1107	34-35	0.1524234513088345
1107	36-37	0.1865786404841714
1107	38-39	0.19997933909380805
1107	40-41	0.18887428065637835
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2216	50-51	-0.43785203777368764
2216	52-53	-0.4720786773333998
2216	54-55	-0.42204817389120564
2216	56-57	-0.47669620923122125
2216	58-59	-0.46227120597026783
2216	60-61	-0.2734075486711163
2216	62-63	-0.19192866166291367
2216	64-65	-0.33018601115978186
2216	66-67	-0.26085295804791997
2216	68-69	-0.17839855220989875
2216	70-71	-0.24789529053234105
2216	72-73	-0.25156877462552885
2216	74-75	-0.35205150073358027
2216	76-77	-0.317527930586472
2216	78-79	-0.17970229919739822
2216	80-81	-0.45547295613517136
2216	82-83	-0.26160916217886054
2216	84-85	-0.330447366809274
2216	86-87	-0.3148981072983048
2216	88-89	-0.23511437982455163
2216	90-91	-0.28657808117572614
2216	92-93	-0.2180402179535399
2216	94-95	-0.3411917806095772
2216	96-97	-0.505204695306869
2216	98-99	-0.3695938756888104
2216	100-101	-0.46883609601073317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	287.0
3	87.0
4	185.0
5	362.0
6	949.0
7	2315.0
8	5541.0
9	11023.0
10	19752.0
11	26519.0
12	30068.0
13	45042.0
14	75044.0
15	128060.0
16	153854.0
17	223353.0
18	210515.0
19	96401.0
20	64211.0
21	53681.0
22	51299.0
23	54333.0
24	64363.0
25	81262.0
26	107510.0
27	139707.0
28	177007.0
29	208962.0
30	258990.0
31	329251.0
32	437147.0
33	601598.0
34	839614.0
35	1193556.0
36	1860759.0
37	3138755.0
38	3048047.0
39	748874.0
40	584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.661148654762695	44.61000199349675	12.510829191036807	19.218020160703745
2	22.009213940768866	33.08496658217356	29.2316764521013	15.674143024956274
3	19.519663485326795	25.4396045927743	30.568925882472058	24.47180603942684
4	21.792711604019832	23.55866749277221	33.067437226112986	21.581183677094973
5	21.28814753988491	24.621747068271024	22.656192247953722	31.433913143890347
6	24.932543209685672	23.43463458759872	24.69352661904924	26.939295583666368
7	24.205248303608958	27.548237830883394	21.8083104007355	26.438203464772148
8	26.376389541017943	24.649712085838043	23.75307192757032	25.220826445573696
9	24.406946381659793	24.536742590017564	27.366473858860047	23.689837169462592
10-11	27.28105310097746	23.70645682647235	25.07434501262245	23.938145059927738
12-13	25.250375918028357	25.3258614797496	24.865425090104548	24.558337512117497
14-15	24.08623117321734	24.838922877820604	24.84997619206526	26.224869756896794
16-17	24.749723356560594	27.152661419281436	23.43809560816591	24.659519615992057
18-19	25.839739573839694	26.295033973325864	25.555956169657712	22.309270283176733
20-21	24.712605202325342	24.817116479846216	27.411694786072644	23.058583531755797
22-23	20.414053079512705	25.664877729914977	29.425126892254582	24.495942298317736
24-25	20.882550719804385	24.36595628905973	31.63930968515343	23.112183305982448
26-27	20.080660173644972	24.067903606464547	32.96841462701684	22.88302159287364
28-29	22.3618209760639	24.539524035937387	32.71426606373017	20.384388924268542
30-31	20.871379385348966	24.559193098447285	33.31795944821177	21.251468067991983
32-33	20.817138427732136	24.611317089183025	33.705130290725975	20.866414192358864
34-35	20.630074111384967	24.41820330050652	33.5122994779371	21.439423110171415
36-37	21.750928293876967	24.57337166682029	32.7645714764113	20.91112856289144
38-39	20.79036269709702	24.736123259327318	32.79844103752212	21.675073006053545
40-41	21.817782820372205	25.188608112454965	31.976891996993356	21.016717070179478
42-43	21.653106270856128	24.930767595808028	31.519119435115623	21.897006698220224
44-45	21.56273502959203	26.497451457039396	29.32090894339771	22.61890456997086
46-47	22.34512539857948	26.199620792591933	28.674642431957558	22.780611376871025
48-49	23.671716359878243	26.05682694167874	27.704129660379934	22.567327038063087
50-51	23.681693074716687	25.64738623128937	27.351792413460313	23.319128280533626
52-53	22.993316178552817	26.504066881143984	27.224854779880303	23.277762160422895
54-55	24.011397854642464	25.710136617307622	27.195421836641888	23.08304369140803
56-57	22.79088489113745	26.23514316198775	27.67877571103386	23.29519623584094
58-59	22.335131518565255	27.03069536078977	27.34664829209903	23.287524828545944
60-61	23.46073367917588	25.997384750650276	26.504991729166953	24.036889841006893
62-63	23.74894807164701	25.927872759132924	27.249946458891504	23.073232710328558
64-65	22.0139469842604	27.524257072688986	27.56398412657111	22.897811816479507
66-67	22.019117392501077	26.25022465634826	28.04171236864678	23.688945582503887
68-69	23.062648721946307	26.59115443602319	27.1988831148771	23.147313727153406
70-71	22.61924300784826	27.359268003679528	27.164541947388937	22.856947041083274
72-73	22.982508673124983	27.076607407487675	27.124813386885716	22.816070532501627
74-75	22.6575577393478	26.831498096158406	26.971381398250504	23.539562766243286
76-77	22.625091346091462	27.299426483202392	26.90864327967461	23.16683889103153
78-79	23.02321499672818	26.92365800583303	26.925121198227576	23.128005799211216
80-81	21.955704117165574	26.937509625927525	27.39985437781231	23.70693187909459
82-83	22.006655614926242	28.239831003479303	26.02021945335917	23.733293928235284
84-85	22.622524078899843	26.559503013867737	26.268436298735963	24.54953660849646
86-87	22.293098126650943	26.792061595019128	27.005275972233267	23.909564306096666
88-89	22.163442048969674	28.223589030537916	26.219741030042915	23.393227890449488
90-91	22.927688431873875	27.177135273774823	25.605009175848696	24.290167118502602
92-93	24.36921111686423	26.685651265442832	25.737350782627257	23.20778683506569
94-95	23.13796644467131	27.74547527743208	26.418023859988843	22.69853441790777
96-97	21.78120266063433	28.768092293078368	26.1628145131821	23.28789053310521
98-99	23.981917004446277	26.95275104622617	25.894279263714353	23.1710526856132
100-101	23.438169268521555	27.090295336400626	26.0620124584799	23.409522936597913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	479.0
1	380.5
2	262.5
3	213.0
4	166.5
5	163.5
6	188.5
7	184.0
8	194.5
9	200.0
10	221.0
11	269.5
12	350.0
13	770.0
14	1075.0
15	1230.5
16	7932.5
17	9799.0
18	5240.5
19	5805.0
20	7362.5
21	9925.5
22	13706.5
23	19115.5
24	25102.0
25	32764.5
26	44010.0
27	60459.0
28	82698.0
29	113123.5
30	152857.0
31	191355.5
32	228522.5
33	278387.5
34	343053.0
35	400211.5
36	425819.5
37	441782.0
38	454033.0
39	460521.0
40	461244.5
41	451674.5
42	435896.0
43	447609.5
44	470911.5
45	469914.5
46	474555.5
47	485022.0
48	531415.5
49	582808.0
50	535966.0
51	506092.5
52	561365.5
53	593295.0
54	530164.5
55	507436.5
56	504176.0
57	446686.0
58	365959.5
59	275242.5
60	234696.5
61	191577.5
62	152226.5
63	117861.0
64	85409.0
65	60862.5
66	43936.5
67	31426.5
68	23371.0
69	18695.0
70	15205.5
71	12021.5
72	10578.0
73	8915.5
74	6622.0
75	5385.0
76	4515.5
77	3520.0
78	2357.0
79	1266.5
80	514.0
81	306.5
82	256.5
83	121.0
84	27.0
85	8.0
86	8.0
87	7.5
88	7.5
89	6.5
90	7.0
91	5.0
92	1.5
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08139352787212416
2	0.011657226199950625
3	7.453998991087433E-4
4	0.0
5	0.005134977082749121
6	0.002298316355585292
7	0.0052247011446788765
8	0.0
9	0.0
10-11	0.0
12-13	2.4156478211857422E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.00552148073413884
28-29	0.0
30-31	7.937128555324581E-5
32-33	0.0
34-35	0.0
36-37	3.8305272593088197E-4
38-39	0.0
40-41	0.0020256932443371866
42-43	0.0011560600287103195
44-45	0.0
46-47	0.0015598183073942222
48-49	0.0
50-51	5.8665732800225165E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	4.90031415154822E-4
68-69	0.0
70-71	3.51994396801351E-4
72-73	3.450925458836774E-6
74-75	2.7607403670694194E-5
76-77	1.0352776376510325E-4
78-79	0.0
80-81	2.4156478211857422E-5
82-83	3.3128884404833036E-4
84-85	6.384212098848032E-4
86-87	0.0011250016995807885
88-89	3.002305149187994E-4
90-91	1.1388054014161357E-4
92-93	7.246943463557227E-4
94-95	3.485434713425142E-4
96-97	4.5897308602529095E-4
98-99	4.486203096487807E-5
100-101	6.970869426850284E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4488867E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.99961854951936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83247872284788	25.782753502413712
2	12.658784564369817	8.607876929782348
3	4.215458672158206	4.299719605939272
4	1.8902663890685314	2.5707334474122967
5	1.0379945698746758	1.7645709716105702
6	0.6644734969573345	1.3555107259688755
7	0.4749021525255981	1.13025444239512
8	0.3577918962341661	0.9731830393656687
9	0.27997261198400125	0.8567065810591771
>10	2.013215318024596	13.401702711351327
>50	0.28365249854186314	6.8886834303670135
>100	0.27001662173555213	17.425921746070163
>500	0.016412542373648416	3.73156050637111
>1k	0.0036232529451498603	2.3078070652709006
>5k	2.442613682587244E-4	0.5892735326103012
>10k+	7.124289907546129E-4	8.313741762011892
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	90427	0.624113672932466	No Hit
AATTCGACTGTCACCGAAGTGCGGCAAAGACAGCAACGGCAATACACACA	80619	0.5564203191319238	No Hit
AATTCCCCTTCAGTAGAAGGGCACACCGGTATCCCCGCCCGACAACGACG	77717	0.5363911477688352	No Hit
AATTCCCCTTGGTTGCAAGGGAACGATGCGTAATGTGTGTATTGCCGTTG	77096	0.5321050983489599	No Hit
AATTCGAACCTCTGTAACACTCAGACCACGCTGATGCCCAGCGCCTGTTT	71977	0.496774523501389	No Hit
AATTCCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGG	69327	0.4784846185695541	No Hit
AATTCCCCTTGATCAGAAGGGGGACGCTGCCGGATTAATCTGTATACCTG	54814	0.3783180562013579	No Hit
AATTCCCCTTCTACTGAAGGGACAGGTGTTCTCCCGTATTGTTGACATGC	52380	0.3615189510677405	No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT	52268	0.36074594376496105	No Hit
AATTCCCCTTGATTGCGGGGCTATTTTCAAGTGAAACCGGGTACATTGCC	47768	0.32968761463543006	No Hit
AATTCCCCTTGATCAGAAGGGCTGACCTGCTTACTGATTTGTAAAACCGG	47391	0.32708561683946713	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41696	0.2877795758633163	No Hit
AATTCAGTCATCTGAAAGGGTTAACGTGAAGTACCGTTATGAGCTGACGG	40597	0.2801944417047931	No Hit
AATTCCCCTTGTTCCGAAGGGAACCCCAGGGAAGCCATCACCCCCACACT	31376	0.21655247439292527	No Hit
AATTCCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG	28047	0.19357621268799002	No Hit
AATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGA	24988	0.17246345073082664	No Hit
AATTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC	23635	0.16312524643921433	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	23405	0.1615378207281494	TruSeq Adapter, Index 12 (100% over 50bp)
AATTCCATGTAAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC	23007	0.15879088406291533	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	22245	0.1535316736636481	No Hit
AATTCGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA	21613	0.14916970388367842	No Hit
AATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAA	21389	0.14762368927811956	No Hit
AATTCCCCTTGTTCCGAAGGGTAAAACCGGTCCGGCCATCACGCTCACAT	19938	0.13760910359657522	No Hit
AATTCCCCTTGTGCAGAAGGGCCCGTAGAAAAGATCAAAGGATCTTCTTG	18220	0.12575172372001206	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	16602	0.11458452893521626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.901850917673549E-5	0.0	0.0	0.0	0.0
2	8.282221101208259E-5	0.0	0.0	0.0	0.0
3	8.972406192975613E-5	0.0	0.0	0.0	0.0
4	1.0352776376510323E-4	0.0	0.0	0.0	0.0
5	1.3113516743579743E-4	0.0	0.0	0.0	0.0
6	2.277610802832271E-4	0.0	0.0	0.0	0.0
7	2.898777385422891E-4	0.0	0.0	0.0	0.0
8	3.105832912953097E-4	0.0	0.0	0.0	0.0
9	3.8650365138971876E-4	0.0	0.0	0.0	1.3803701835347098E-5
10-11	5.65951775249231E-4	0.0	0.0	0.0	1.3803701835347098E-5
12-13	8.661822901680304E-4	3.4509254588367746E-6	0.0	0.0	1.3803701835347098E-5
14-15	0.0010456304140275428	1.3803701835347098E-5	0.0	0.0	1.3803701835347098E-5
16-17	0.0012768424197696065	3.4509254588367746E-5	0.0	0.0	1.3803701835347098E-5
18-19	0.0015632692328530588	5.5214807341388394E-5	0.0	0.0	1.3803701835347098E-5
20-21	0.0018945580769013893	8.627313647091936E-5	0.0	0.0	2.0705552753020648E-5
22-23	0.0022672580264557606	2.7607403670694197E-4	0.0	0.0	2.0705552753020648E-5
24-25	0.0028711699817521963	4.3136568235459683E-4	0.0	0.0	2.0705552753020648E-5
26-27	0.004006524457709495	5.625008497903942E-4	0.0	0.0	2.7607403670694197E-5
28-29	0.005490422405009308	7.108906445203755E-4	0.0	0.0	2.7607403670694197E-5
30-31	0.008016499840877827	7.799091536971111E-4	0.0	0.0	3.4509254588367746E-5
32-33	0.011457072523338092	7.971637809912949E-4	0.0	3.4509254588367746E-6	4.1411105506041295E-5
34-35	0.016519580171451637	8.006147064501316E-4	0.0	6.901850917673549E-6	5.1763881882551616E-5
36-37	0.023390372759995656	8.006147064501316E-4	0.0	6.901850917673549E-6	6.901850917673549E-5
38-39	0.03228685859287686	8.075165573678052E-4	0.0	6.901850917673549E-6	6.901850917673549E-5
40-41	0.044478978238947184	8.075165573678052E-4	0.0	6.901850917673549E-6	7.592036009440903E-5
42-43	0.05950085676126367	8.075165573678052E-4	0.0	6.901850917673549E-6	8.627313647091936E-5
44-45	0.07789428945686368	8.075165573678052E-4	0.0	6.901850917673549E-6	8.972406192975613E-5
46-47	0.10085329653450473	8.075165573678052E-4	0.0	6.901850917673549E-6	9.317498738859291E-5
48-49	0.12854697334166987	8.178693337443155E-4	0.0	6.901850917673549E-6	9.662591284742969E-5
50-51	0.1602506255320033	8.282221101208259E-4	0.0	6.901850917673549E-6	1.0352776376510323E-4
52-53	0.1969719233394854	8.282221101208259E-4	0.0	6.901850917673549E-6	1.0352776376510323E-4
54-55	0.24035005635706366	8.282221101208259E-4	0.0	6.901850917673549E-6	1.0352776376510323E-4
56-57	0.2875483638575742	8.282221101208259E-4	0.0	6.901850917673549E-6	1.0352776376510323E-4
58-59	0.3418314213250767	8.316730355796627E-4	0.0	6.901850917673549E-6	1.0352776376510323E-4
60-61	0.4015048243592822	8.385748864973362E-4	0.0	6.901850917673549E-6	1.1042961468277679E-4
62-63	0.46681703959322696	8.42025811956173E-4	0.0	6.901850917673549E-6	1.1042961468277679E-4
64-65	0.540011168575155	8.42025811956173E-4	0.0	1.0352776376510324E-5	1.1388054014161355E-4
66-67	0.6193824541284008	8.42025811956173E-4	0.0	1.7254627294183873E-5	1.1733146560045033E-4
68-69	0.7076847347691162	8.42025811956173E-4	0.0	2.0705552753020648E-5	1.1733146560045033E-4
70-71	0.8033202320098598	8.42025811956173E-4	0.0	2.0705552753020648E-5	1.2078239105928711E-4
72-73	0.906402826390773	8.42025811956173E-4	0.0	2.0705552753020648E-5	1.2423331651812387E-4
74-75	1.0181852038534138	8.42025811956173E-4	0.0	2.7607403670694197E-5	1.3113516743579743E-4
76-77	1.1414246538393926	8.558295137915201E-4	0.0	3.1058329129530975E-5	1.3113516743579743E-4
78-79	1.2725908796043197	8.558295137915201E-4	0.0	3.796018004720452E-5	1.3803701835347098E-4
80-81	1.4135094207159193	8.627313647091937E-4	0.0	4.1411105506041295E-5	1.3803701835347098E-4
82-83	1.56400773090125	8.627313647091937E-4	0.0	4.4862030964878073E-5	1.3803701835347098E-4
84-85	1.723626837074286	8.765350665445407E-4	0.0	5.5214807341388394E-5	1.483897947299813E-4
86-87	1.895044657391085	8.765350665445407E-4	0.0	5.8665732800225165E-5	1.5184072018881807E-4
88-89	2.0770499170155956	8.765350665445407E-4	0.0	6.211665825906194E-5	1.621934965653284E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	32685	0.0	87.783325	1
AATTCGA	24200	0.0	87.12354	1
AATTCCC	60055	0.0	84.8712	1
ATTCCCC	58920	0.0	84.24141	2
TCGACTG	9470	0.0	78.70635	4
TCCCCTT	59890	0.0	77.53526	4
TTCCCCT	61630	0.0	75.3547	3
ATTCGAA	11020	0.0	73.034584	2
TCGACGT	1425	0.0	72.00169	7
CCCCTTG	45950	0.0	71.70681	5
TTCGACT	10460	0.0	71.16699	3
TTCGAAC	9975	0.0	71.05542	3
ATTCCGA	9925	0.0	70.9438	2
TCGACGG	19360	0.0	70.89881	2
AATTCCG	10095	0.0	70.32483	1
GTCGACG	19665	0.0	69.27649	1
CACTACG	3780	0.0	68.73412	8
ATTCGAG	8055	0.0	67.83113	2
ATTCGAC	11330	0.0	65.920334	2
TCGAACC	10845	0.0	65.6174	4
>>END_MODULE