FastQCFastQC Report
Tue 31 May 2016
SRR2048254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6850826
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT180630.26366163729745873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG249550.089.363491
TATCTCG101100.083.38232
ATATCTC104950.080.447531
TCGACGG278600.080.216792
ATCTCGA108400.077.942423
CGACGGC287250.077.784673
CGGCGCG295900.075.498196
GACGGCG300650.074.490574
ACGGCGC300900.074.387865
TCTCGAG77900.074.297624
AGGGCGC80050.071.72919
TCGAGGG81900.070.179136
CTCGAGG82450.070.131175
CTCGACG37900.069.827615
CGAGGGC83500.068.8338857
CGCGCCG366950.060.985829
TCTCGAC44150.059.9500584
GAGGGCG96200.059.939368
GCGCGCC374600.059.7836468
GGCGCGC375700.059.7019047
Produced by FastQC (version 0.11.5)