##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048254_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6850826 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.192090267655317 31.0 31.0 34.0 28.0 34.0 2 31.27886797883934 31.0 31.0 34.0 28.0 34.0 3 31.44082538368366 31.0 31.0 34.0 28.0 34.0 4 35.18873943083651 37.0 35.0 37.0 32.0 37.0 5 35.138155895362104 37.0 35.0 37.0 32.0 37.0 6 35.15387721130269 37.0 35.0 37.0 32.0 37.0 7 35.12467547708846 37.0 35.0 37.0 32.0 37.0 8 35.12522329424218 37.0 35.0 37.0 32.0 37.0 9 36.69253371783198 39.0 37.0 39.0 32.0 39.0 10-11 36.631550341520864 39.0 37.0 39.0 32.0 39.0 12-13 36.60568024935971 39.0 37.0 39.0 32.0 39.0 14-15 37.80317468287765 40.0 37.0 41.0 32.0 41.0 16-17 37.812511002322935 40.0 37.5 41.0 32.0 41.0 18-19 37.817391654670544 40.0 37.0 41.0 32.0 41.0 20-21 37.77954738596485 40.0 37.0 41.0 32.0 41.0 22-23 37.81694937223628 40.0 37.0 41.0 32.0 41.0 24-25 37.795562170167514 40.0 37.0 41.0 32.0 41.0 26-27 37.79517477162608 40.0 37.0 41.0 32.0 41.0 28-29 37.587040161288584 40.0 37.0 41.0 31.5 41.0 30-31 37.43825875887083 40.0 36.5 41.0 31.0 41.0 32-33 37.36337610968371 40.0 36.0 41.0 31.0 41.0 34-35 37.08834175616196 39.5 35.5 41.0 30.5 41.0 36-37 37.00210047664325 39.0 35.0 41.0 30.0 41.0 38-39 36.86931831285746 39.0 35.0 41.0 30.0 41.0 40-41 36.487910435909484 39.0 35.0 41.0 29.5 41.0 42-43 36.270042473710475 39.0 35.0 40.0 29.0 41.0 44-45 36.09684168011273 38.0 35.0 40.0 28.0 41.0 46-47 36.008273454908945 38.0 34.0 40.0 28.0 41.0 48-49 35.8960142178476 38.0 34.0 40.0 28.0 41.0 50-51 35.841770247850405 38.0 34.0 40.0 28.0 41.0 52-53 35.655878502825786 38.0 34.0 40.0 28.0 41.0 54-55 35.594016473342045 38.0 34.0 40.0 28.0 41.0 56-57 35.512584030013315 38.0 34.0 40.0 28.0 41.0 58-59 35.389062866287944 38.0 34.0 40.0 28.0 41.0 60-61 35.14355743380433 37.0 33.5 40.0 28.0 41.0 62-63 34.827475329252266 37.0 33.0 40.0 27.0 41.0 64-65 34.4773973970438 36.0 33.0 39.5 26.5 41.0 66-67 34.140531886228025 36.0 33.0 39.0 26.0 41.0 68-69 33.793241136178324 35.0 32.5 39.0 26.0 40.5 70-71 33.41679288015781 35.0 32.0 38.0 26.0 40.0 72-73 33.14748936843528 35.0 32.0 37.0 26.0 40.0 74-75 33.12958758549699 35.0 32.5 37.0 26.0 39.0 76-77 32.86999363288456 35.0 32.5 36.5 26.0 39.0 78-79 32.5795517212085 35.0 32.0 36.0 26.0 38.5 80-81 32.30403392233286 35.0 32.0 36.0 26.0 37.0 82-83 32.03502103250032 35.0 32.0 35.0 25.0 37.0 84-85 31.818987666596698 35.0 32.0 35.0 25.0 36.5 86-87 31.623200618436375 35.0 32.0 35.0 25.0 36.0 88-89 31.451353092313248 35.0 32.0 35.0 25.0 36.0 90-91 31.27624106932507 35.0 32.0 35.0 24.5 36.0 92-93 31.11661228879554 35.0 32.0 35.0 24.0 35.0 94-95 30.968301194629667 35.0 32.0 35.0 24.0 35.0 96-97 30.795337744674875 34.0 32.0 35.0 23.0 35.0 98-99 30.607154524140594 34.0 32.0 35.0 21.0 35.0 100-101 29.990150165834017 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5166127284752022 1101 2 0.5272409823666102 1101 3 0.5708655063677917 1101 4 0.47117873786103104 1101 5 0.4681178776885915 1101 6 0.5355103068841771 1101 7 0.5223560694581053 1101 8 0.5609705570663905 1101 9 0.6534936332557777 1101 10-11 0.6432674450362867 1101 12-13 0.6455556533580662 1101 14-15 0.76824287533465 1101 16-17 0.7774616647203132 1101 18-19 0.8101864456948888 1101 20-21 0.8154465612743422 1101 22-23 0.7722893160956588 1101 24-25 0.7218255318590039 1101 26-27 0.7723254770683781 1101 28-29 0.8840451328629513 1101 30-31 0.7995790645234351 1101 32-33 0.864417301362792 1101 34-35 0.9160684333041189 1101 36-37 0.9038067438508364 1101 38-39 0.8233390673345937 1101 40-41 0.7537956037792028 1101 42-43 0.8009709678101729 1101 44-45 0.7808315355002975 1101 46-47 0.7282115826126798 1101 48-49 0.7932418161969181 1101 50-51 0.7909650188244015 1101 52-53 0.7409830431802646 1101 54-55 0.5334505670745102 1101 56-57 0.5009766708858692 1101 58-59 0.5357329869961163 1101 60-61 0.5286438049808169 1101 62-63 0.4859599748935608 1101 64-65 0.48522966878486784 1101 66-67 0.5259989895281976 1101 68-69 0.45253676137173215 1101 70-71 0.49338428454399974 1101 72-73 0.46609377696529464 1101 74-75 0.4710528466151729 1101 76-77 0.4951970673391912 1101 78-79 0.5434241074993551 1101 80-81 0.47425506943905305 1101 82-83 0.4091755818543916 1101 84-85 0.440331826275159 1101 86-87 0.4113365357785419 1101 88-89 0.48273691443762345 1101 90-91 0.5124760752624269 1101 92-93 0.5057242493510792 1101 94-95 0.5000607806563409 1101 96-97 0.49844915763266684 1101 98-99 0.5436812477497774 1101 100-101 0.5465889009463467 1102 1 0.3952938169721705 1102 2 0.408373849150351 1102 3 0.4792526796040093 1102 4 0.38892382519697577 1102 5 0.39134976922405684 1102 6 0.42544333624535824 1102 7 0.453459960618531 1102 8 0.4920891172179793 1102 9 0.606689597501969 1102 10-11 0.5885134030282728 1102 12-13 0.5976455882831431 1102 14-15 0.7293613560292016 1102 16-17 0.693532110144325 1102 18-19 0.7474630716189168 1102 20-21 0.7876077508239661 1102 22-23 0.73545035194104 1102 24-25 0.6499969442208098 1102 26-27 0.6633294931606954 1102 28-29 0.7268078334176238 1102 30-31 0.7165349222983721 1102 32-33 0.6951172160740526 1102 34-35 0.7551752322233796 1102 36-37 0.8014160019556442 1102 38-39 0.7644455126651977 1102 40-41 0.6051443646418875 1102 42-43 0.6424359671364428 1102 44-45 0.6009240766549411 1102 46-47 0.5959385194974942 1102 48-49 0.6280819032860947 1102 50-51 0.6007007671354714 1102 52-53 0.5491035580234396 1102 54-55 0.4434595660931109 1102 56-57 0.4239575693854434 1102 58-59 0.3696851635430889 1102 60-61 0.3350884943029797 1102 62-63 0.369657528508462 1102 64-65 0.34712261594265215 1102 66-67 0.3820693125165846 1102 68-69 0.3450861052336762 1102 70-71 0.37156454632398095 1102 72-73 0.36640260967809724 1102 74-75 0.4090234555256771 1102 76-77 0.4548211833663274 1102 78-79 0.392231679081533 1102 80-81 0.4375477672414476 1102 82-83 0.43087683110429253 1102 84-85 0.40776474496403736 1102 86-87 0.3971455140995097 1102 88-89 0.37010021207650823 1102 90-91 0.3340496218246827 1102 92-93 0.3795211264916709 1102 94-95 0.3300433118476178 1102 96-97 0.33779768930206444 1102 98-99 0.36261552555658483 1102 100-101 0.34888030117363655 1103 1 0.28874549093220025 1103 2 0.3615835148467177 1103 3 0.3589368322106985 1103 4 0.30239959529967564 1103 5 0.34045683911710256 1103 6 0.3970070521205784 1103 7 0.3720389428283255 1103 8 0.44865972278614663 1103 9 0.499112448560453 1103 10-11 0.5113881858452629 1103 12-13 0.4921266188596576 1103 14-15 0.6334780853595134 1103 16-17 0.5892391122489684 1103 18-19 0.6367065120050412 1103 20-21 0.6367569908087134 1103 22-23 0.6129120589104389 1103 24-25 0.5868169282307107 1103 26-27 0.5549834027023621 1103 28-29 0.6223336936203765 1103 30-31 0.5874786805811638 1103 32-33 0.6118915994805221 1103 34-35 0.6617497046270415 1103 36-37 0.6772371353607909 1103 38-39 0.7263271957738553 1103 40-41 0.5889043781848358 1103 42-43 0.6012445621987723 1103 44-45 0.6063766358827252 1103 46-47 0.5667591861146164 1103 48-49 0.5797647853300774 1103 50-51 0.5151521492337352 1103 52-53 0.5053384994102572 1103 54-55 0.43980786494100954 1103 56-57 0.38117178320204914 1103 58-59 0.42512092869858265 1103 60-61 0.37688496665890625 1103 62-63 0.3386846201332432 1103 64-65 0.3062137304043162 1103 66-67 0.32595733999378496 1103 68-69 0.3293253003190131 1103 70-71 0.32242802953633287 1103 72-73 0.3251599492147932 1103 74-75 0.3267745608658217 1103 76-77 0.30329425376888963 1103 78-79 0.31898129367559136 1103 80-81 0.33165388905841553 1103 82-83 0.3340574055585819 1103 84-85 0.3911077146069779 1103 86-87 0.40140413414111364 1103 88-89 0.4017653628825748 1103 90-91 0.4115716725875487 1103 92-93 0.4003013408234608 1103 94-95 0.34023404106440225 1103 96-97 0.38048504522796023 1103 98-99 0.3699696732871942 1103 100-101 0.41999498614906017 1104 1 0.3408540500182937 1104 2 0.2679414897390373 1104 3 0.26100346940133434 1104 4 0.2747885264434444 1104 5 0.26635840153671353 1104 6 0.30666856350743643 1104 7 0.3404853673211221 1104 8 0.3435402171127109 1104 9 0.40098844531118516 1104 10-11 0.39867497741629165 1104 12-13 0.43423784864600634 1104 14-15 0.5092862710438197 1104 16-17 0.47704968999668296 1104 18-19 0.5059148101513387 1104 20-21 0.5500525233195432 1104 22-23 0.5065543081599202 1104 24-25 0.4122550281340125 1104 26-27 0.5190413798287139 1104 28-29 0.5812636476849633 1104 30-31 0.51136817373051 1104 32-33 0.5397840668347271 1104 34-35 0.5956297050483954 1104 36-37 0.6308771769519126 1104 38-39 0.636782219858766 1104 40-41 0.488421204103787 1104 42-43 0.49148127659952934 1104 44-45 0.4161950548300126 1104 46-47 0.4627841226368332 1104 48-49 0.4732921549899203 1104 50-51 0.3992792240188905 1104 52-53 0.45600750917964206 1104 54-55 0.44312745570715606 1104 56-57 0.25773057641669794 1104 58-59 0.2958256131717789 1104 60-61 0.29099577114772757 1104 62-63 0.21614482311125727 1104 64-65 0.20582528159893343 1104 66-67 0.3073620890364168 1104 68-69 0.3087510064168697 1104 70-71 0.34488439046565844 1104 72-73 0.32135788169763657 1104 74-75 0.3131583663017352 1104 76-77 0.2998563244347281 1104 78-79 0.2616415417328639 1104 80-81 0.2281634422862524 1104 82-83 0.246684110011671 1104 84-85 0.23917839424568754 1104 86-87 0.28360893934648956 1104 88-89 0.3088716531555775 1104 90-91 0.26078432701732623 1104 92-93 0.30524065032688696 1104 94-95 0.23006074200502624 1104 96-97 0.26691280643861504 1104 98-99 0.279591890679761 1104 100-101 0.34537477782337334 1105 1 0.219118146508805 1105 2 0.20829118616420317 1105 3 0.2596453055526027 1105 4 0.18252121723273973 1105 5 0.174791900951341 1105 6 0.24183320180414825 1105 7 0.25469168930102626 1105 8 0.18726680917892935 1105 9 0.33940952946812075 1105 10-11 0.34698222781787535 1105 12-13 0.3144018607345487 1105 14-15 0.28462243592716163 1105 16-17 0.3539730475615528 1105 18-19 0.3722961981553681 1105 20-21 0.38290296847187477 1105 22-23 0.3590324317135156 1105 24-25 0.3298452157241982 1105 26-27 0.36309782024365234 1105 28-29 0.4224466638302431 1105 30-31 0.38524881300738656 1105 32-33 0.29183334669533423 1105 34-35 0.4281380227265288 1105 36-37 0.5303223020574137 1105 38-39 0.43606061023150033 1105 40-41 0.36200983978676504 1105 42-43 0.3682406765287354 1105 44-45 0.3152382359675414 1105 46-47 0.3823231245154517 1105 48-49 0.39493731276714783 1105 50-51 0.34890586781535404 1105 52-53 0.3675699802515453 1105 54-55 0.3855818922559351 1105 56-57 0.2624342710449312 1105 58-59 0.229100518559882 1105 60-61 0.15712844560115968 1105 62-63 0.23192995202845879 1105 64-65 0.08305480050769631 1105 66-67 0.25239326331287515 1105 68-69 0.2737423484082129 1105 70-71 0.28183413045313443 1105 72-73 0.2349511186493629 1105 74-75 0.19595999444298684 1105 76-77 0.2089079729958101 1105 78-79 0.1694491596914247 1105 80-81 0.13052805880180784 1105 82-83 0.15638295833316818 1105 84-85 0.1685292614078726 1105 86-87 0.1713812718358021 1105 88-89 0.1397272713319495 1105 90-91 0.1279104126717172 1105 92-93 0.1763353438932782 1105 94-95 0.15523883016424378 1105 96-97 0.1526438091884863 1105 98-99 0.1290881470439409 1105 100-101 0.11942566404406918 1106 1 0.151779685381225 1106 2 0.17519907281858593 1106 3 0.2411993476966643 1106 4 0.16846684124790556 1106 5 0.15743704156507476 1106 6 0.21672426288365898 1106 7 0.17509528943990915 1106 8 0.2271066190232247 1106 9 0.3093196328252219 1106 10-11 0.290635006708456 1106 12-13 0.33917824083229675 1106 14-15 0.4015101318916905 1106 16-17 0.35475006863357805 1106 18-19 0.35262966502501314 1106 20-21 0.42625451615769094 1106 22-23 0.36818172530644233 1106 24-25 0.2914564648228364 1106 26-27 0.27368236208057795 1106 28-29 0.4433721505700916 1106 30-31 0.4247060146205044 1106 32-33 0.4162649894590018 1106 34-35 0.49701028189379315 1106 36-37 0.5076681343892275 1106 38-39 0.4931932652003823 1106 40-41 0.49301486075196266 1106 42-43 0.47620793342588286 1106 44-45 0.46311183049789406 1106 46-47 0.4054765577339481 1106 48-49 0.438409123252427 1106 50-51 0.415657223208882 1106 52-53 0.4995952987356418 1106 54-55 0.41080682081400965 1106 56-57 0.3770724143560997 1106 58-59 0.4014660341435743 1106 60-61 0.4136696148450767 1106 62-63 0.4018142027562348 1106 64-65 0.3254213042600753 1106 66-67 0.40304625586154685 1106 68-69 0.3148184106895542 1106 70-71 0.29310435452705974 1106 72-73 0.22734830904543202 1106 74-75 0.23610482792321363 1106 76-77 0.280454430277544 1106 78-79 0.27326236910306534 1106 80-81 0.2472481399918891 1106 82-83 0.29257949707204745 1106 84-85 0.26617031581056594 1106 86-87 0.21501880087994962 1106 88-89 0.21127917689507214 1106 90-91 0.14123044578871813 1106 92-93 0.20936343993259143 1106 94-95 0.17416968506369201 1106 96-97 0.18801656244009735 1106 98-99 0.20678982693070225 1106 100-101 0.2355099788692705 1107 1 0.04172467273095393 1107 2 0.10611081874409578 1107 3 0.0965952604000968 1107 4 0.066644544231238 1107 5 0.07739298191585675 1107 6 0.059051079501507786 1107 7 0.15758242023251512 1107 8 -0.1906836443679012 1107 9 -0.09473056461083473 1107 10-11 -0.08182249769531325 1107 12-13 -0.03933161816507891 1107 14-15 -0.2081379862150925 1107 16-17 -0.12518477056145372 1107 18-19 -0.020731095524624266 1107 20-21 0.039746767752134815 1107 22-23 -0.015442559188592497 1107 24-25 -0.06465817408346908 1107 26-27 0.04004533550789091 1107 28-29 -0.09161783012470437 1107 30-31 -0.02907739027325107 1107 32-33 -0.010545664801711041 1107 34-35 0.12932659846816819 1107 36-37 0.29287752963954006 1107 38-39 0.1428822510871086 1107 40-41 -0.08039077752214752 1107 42-43 0.03797526065392276 1107 44-45 -0.07572073590312556 1107 46-47 0.028431032269551793 1107 48-49 0.1405765914052992 1107 50-51 -0.00612262543872788 1107 52-53 0.058951566115446496 1107 54-55 0.012945619292182187 1107 56-57 -0.07274362983520888 1107 58-59 0.038680020669254134 1107 60-61 -0.12155457031678907 1107 62-63 -0.09345821127535459 1107 64-65 -0.2597446684448954 1107 66-67 0.017073219642988136 1107 68-69 0.05836503518459324 1107 70-71 0.16272236788793037 1107 72-73 0.20568727974273315 1107 74-75 0.19095808051579866 1107 76-77 0.2538369285576607 1107 78-79 0.25906109073613237 1107 80-81 0.12749340593698122 1107 82-83 0.14378887263774942 1107 84-85 0.20511592052665506 1107 86-87 0.15219711699639404 1107 88-89 0.16210967937583476 1107 90-91 0.14482227767245348 1107 92-93 0.19194222055280363 1107 94-95 0.19550311385677688 1107 96-97 0.10541849143613646 1107 98-99 0.08272776264109893 1107 100-101 0.16116875301472433 1108 1 -0.20251907918741097 1108 2 -0.11298224539515545 1108 3 -0.008481618935018531 1108 4 -0.12255359736339955 1108 5 -0.003510327473371433 1108 6 0.08177600169322119 1108 7 0.06519088864261136 1108 8 0.14095459712521574 1108 9 0.21043252938761015 1108 10-11 0.15337739524047578 1108 12-13 0.20536884140244638 1108 14-15 0.2632395422850067 1108 16-17 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-0.9726131028499623 2215 28-29 -0.867569715386594 2215 30-31 -1.3388114972214922 2215 32-33 -1.1935205962485895 2215 34-35 -1.3197877826753626 2215 36-37 -1.4194401450626657 2215 38-39 -1.1821394114628276 2215 40-41 -1.3603273985254276 2215 42-43 -1.6794853241220906 2215 44-45 -1.178131652306675 2215 46-47 -1.0724971379859056 2215 48-49 -1.039595174487424 2215 50-51 -0.9508671010532908 2215 52-53 -0.8624091931945941 2215 54-55 -0.8486549013225471 2215 56-57 -0.7611455098423718 2215 58-59 -0.6890732529988455 2215 60-61 -0.715426820696706 2215 62-63 -0.7420359878782463 2215 64-65 -0.710200758663575 2215 66-67 -0.7872401025619453 2215 68-69 -0.6522850949153565 2215 70-71 -0.5369354757948344 2215 72-73 -0.5565594573350126 2215 74-75 -0.514820025081903 2215 76-77 -0.5921049351287166 2215 78-79 -0.6042753223565214 2215 80-81 -0.6819732970936201 2215 82-83 -0.7241354276089105 2215 84-85 -0.7901449982732203 2215 86-87 -0.7551736690471564 2215 88-89 -0.7589218229299028 2215 90-91 -0.7595452766655235 2215 92-93 -0.6320600352150834 2215 94-95 -0.6632052527080852 2215 96-97 -0.6896714093818552 2215 98-99 -0.7855585608419489 2215 100-101 -0.7022865607091582 2216 1 -0.5835231836783414 2216 2 -0.5570341850288436 2216 3 -0.6097699165480464 2216 4 -0.49114783543954843 2216 5 -0.49205800918895903 2216 6 -0.5750723114946439 2216 7 -0.8149071365908682 2216 8 -1.7932239346132803 2216 9 -1.1411429165171896 2216 10-11 -0.9510210897405784 2216 12-13 -0.9294130766273128 2216 14-15 -1.7324903918190557 2216 16-17 -1.0435437665277476 2216 18-19 -1.0909359650245634 2216 20-21 -1.0323368492601475 2216 22-23 -0.8843447417266859 2216 24-25 -1.1496154063949078 2216 26-27 -1.3652013356464465 2216 28-29 -1.9933090715911703 2216 30-31 -1.1917688821024015 2216 32-33 -1.1328116481855233 2216 34-35 -2.4406317738697965 2216 36-37 -2.4256172700434533 2216 38-39 -2.53701091281345 2216 40-41 -1.5128864854130626 2216 42-43 -1.3494113621219128 2216 44-45 -1.1591176567632715 2216 46-47 -1.4351932501621292 2216 48-49 -1.4794386786555336 2216 50-51 -0.9659685746810354 2216 52-53 -0.8727698344045862 2216 54-55 -0.8279861176923475 2216 56-57 -0.8734503130738531 2216 58-59 -0.939216807004648 2216 60-61 -1.0642251226416803 2216 62-63 -0.9369550231667461 2216 64-65 -0.9795871580381998 2216 66-67 -0.9911788024055426 2216 68-69 -1.0786514391889455 2216 70-71 -1.1602678877648813 2216 72-73 -0.9052647555382691 2216 74-75 -0.8380048745903466 2216 76-77 -1.000757124971031 2216 78-79 -0.9982991540661175 2216 80-81 -1.0055087551117197 2216 82-83 -1.2692350830330525 2216 84-85 -0.9759234469062363 2216 86-87 -1.1202073772807601 2216 88-89 -0.924372343303979 2216 90-91 -1.1140488450740165 2216 92-93 -1.403926418059818 2216 94-95 -1.025185413058928 2216 96-97 -0.8837791685289424 2216 98-99 -0.8617177076777587 2216 100-101 -0.8626507101062195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 63.0 11 1218.0 12 5003.0 13 10308.0 14 14853.0 15 18064.0 16 20012.0 17 23037.0 18 29758.0 19 41970.0 20 41893.0 21 43366.0 22 50089.0 23 58704.0 24 67114.0 25 76529.0 26 90074.0 27 107280.0 28 126237.0 29 147279.0 30 170957.0 31 201623.0 32 247143.0 33 309310.0 34 403192.0 35 571435.0 36 933080.0 37 1463859.0 38 1330829.0 39 246341.0 40 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.917690341414186 39.1561671981229 13.493880011319161 19.43226244914375 2 23.62108010986148 27.842464313126253 31.665770040900153 16.870685536112116 3 22.170408572477562 27.97211263483581 23.91691038440565 25.940568408280978 4 25.36602606840821 26.234072369079236 25.66760406222593 22.73229750028662 5 22.869776386939815 29.271567277405484 24.85210988917916 23.006546446475536 6 22.3676978187566 25.380012050132873 26.526380578934866 25.725909552175658 7 26.15536564587985 26.13050235383691 24.32199350167276 23.392138498610475 8 26.346134194445014 25.083984665827746 25.920152524211463 22.649728615515777 9 21.78042453508406 26.156755704972102 26.17519191982889 25.88762784011496 10-11 25.100103762111875 25.872944290248395 25.09295857624107 23.93399337139866 12-13 24.525536609489823 25.860141425984036 25.112395763876645 24.5019262006495 14-15 24.39068483724652 25.763272882239296 25.330757846829638 24.515284433684542 16-17 24.56379341174238 27.421011338050526 25.171340677947544 22.843854572259545 18-19 22.734663786872666 25.671945648352512 26.98507741118946 24.608313153585357 20-21 22.67706650424371 27.91909701985752 25.464301315501842 23.939535160396925 22-23 22.077958291193735 27.511176498712906 27.71222755858697 22.698637651506388 24-25 22.0260855403607 25.94530391874389 29.21737320084587 22.811237340049537 26-27 21.99629453450774 25.757795283797734 30.201901374006106 22.044008807688414 28-29 22.05802367994964 25.70945208828296 30.314851106288955 21.917673125478444 30-31 22.107561622196304 25.621035270560068 30.239380124813188 22.03202298243044 32-33 22.076556065311685 25.537799122056843 30.350575112399657 22.035069700231816 34-35 22.132721902296712 25.809818060293132 30.206558680950813 21.850901356459346 36-37 22.124755168645706 25.763834412990093 30.037358586081233 22.07405183228297 38-39 22.23529895222255 25.712207141242477 29.940024085545954 22.112469820989023 40-41 22.78265078580432 26.304711497821504 28.298823211197018 22.613814505177157 42-43 22.82166119618106 26.433838459182724 28.155421152459997 22.589079192176218 44-45 22.93083086587701 26.677547340748802 27.835702697185393 22.55591909618879 46-47 22.87121290191869 27.063218362282154 27.62596802195823 22.439600713840928 48-49 22.878657259723134 27.14385097505031 27.39480611535018 22.58268564987638 50-51 22.80711972508579 27.00318447791269 27.571326692575447 22.618369104426076 52-53 22.823082327384174 27.145300285622078 27.477676971609018 22.553940415384734 54-55 22.879682949793086 26.976119054396296 27.460963558202504 22.68323443760811 56-57 22.8322176041254 26.826932985891045 27.7464790377102 22.59437037227336 58-59 22.9215204829374 26.98550447708556 27.592818299467286 22.500156740509755 60-61 22.84532551257323 26.958223723679453 27.54367137626908 22.65277938747824 62-63 22.858148783810886 26.87379594810903 27.696083654730103 22.57197161334998 64-65 22.998066176243046 27.053578362193747 27.41456463566515 22.533790825898055 66-67 23.01100995343464 26.970787795470923 27.277438068920702 22.740764182173734 68-69 23.007539528810103 26.974542923729196 27.267157274173947 22.750760273286755 70-71 23.10549942498942 27.188808046893016 27.05900461752801 22.64668791058956 72-73 23.076715347981544 27.193532987083625 26.866826452142778 22.862925212792053 74-75 23.026207988847506 27.131528960530183 27.049841343408044 22.792421707214267 76-77 23.135977828779993 27.299212355906704 26.872824213293768 22.691985602019535 78-79 23.172418172144482 27.20023821983224 26.8251660568852 22.802177551138076 80-81 23.148344125323323 27.130238104969372 26.941419638113146 22.77999813159416 82-83 23.230633603008833 27.30261710207671 26.763932696854493 22.702816598059968 84-85 23.276521692420737 27.162702716431564 26.78582115499649 22.774954436151205 86-87 23.288680810168 27.091426639649 26.89695373959286 22.72293881059014 88-89 23.34593108523929 27.231521223245288 26.69734472144999 22.725202970065432 90-91 23.420956830606997 27.20500418489683 26.59390269144188 22.780136293054298 92-93 23.336996151996853 27.124838669088952 26.745745695482558 22.792419483431633 94-95 23.48131451594304 27.298554947972697 26.53539879716694 22.68473173891732 96-97 23.487197018286555 27.27787131070035 26.46692530214605 22.768006368867052 98-99 23.506523155018098 27.19583010866135 26.62501572803046 22.672631008290097 100-101 23.67133539809652 27.39164591247829 26.265029939455474 22.671988749969714 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5358.0 1 4025.5 2 2218.0 3 1464.0 4 1029.0 5 767.5 6 568.5 7 451.5 8 392.0 9 333.0 10 275.5 11 245.5 12 249.0 13 295.5 14 363.0 15 532.0 16 5298.0 17 5949.5 18 2139.5 19 2661.0 20 3750.5 21 5321.5 22 7488.0 23 10397.5 24 13471.0 25 17365.5 26 22897.5 27 30085.0 28 37697.0 29 48275.5 30 63030.0 31 77874.0 32 94662.0 33 118053.5 34 142648.5 35 166292.5 36 187907.5 37 203484.5 38 216570.0 39 231329.0 40 238329.5 41 234263.5 42 230248.0 43 232917.5 44 239601.0 45 249033.5 46 256086.0 47 259199.5 48 259751.0 49 254612.5 50 246808.5 51 241094.0 52 241389.0 53 239952.5 54 227975.0 55 213297.0 56 198702.0 57 176879.5 58 156512.5 59 141374.5 60 124782.5 61 106234.5 62 86656.5 63 68132.5 64 52225.5 65 38141.5 66 27217.0 67 19805.0 68 14662.0 69 11146.0 70 8727.5 71 6700.5 72 5316.5 73 3923.0 74 2792.5 75 2110.5 76 1483.5 77 934.0 78 577.0 79 339.0 80 160.0 81 61.0 82 28.5 83 17.0 84 12.5 85 7.0 86 3.5 87 2.5 88 2.5 89 2.0 90 0.5 91 0.5 92 2.0 93 3.0 94 3.5 95 5.5 96 5.0 97 4.0 98 3.0 99 1.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0846175337105336 2 0.04276856542554139 3 0.049979374749847684 4 0.05606623201348276 5 0.04139646810472197 6 0.016187829029667374 7 0.02001218539195128 8 0.011181133486677373 9 0.0026128236215603785 10-11 8.685084105186732E-4 12-13 4.962905202963847E-4 14-15 0.0011896375707104515 16-17 1.4596780008717196E-5 18-19 0.001525363510910947 20-21 0.005816816833473803 22-23 0.01566964333935791 24-25 0.019304241561528494 26-27 0.00984552811587975 28-29 0.017392063380386538 30-31 4.0141145023972296E-4 32-33 0.005583268353334328 34-35 1.8245975010896496E-4 36-37 0.0028025817616737017 38-39 0.009626576415748992 40-41 0.0015545570709283815 42-43 0.0018100007210809326 44-45 0.0021530250512857866 46-47 0.0 48-49 0.0 50-51 0.003685686952201092 52-53 0.0024084687014383375 54-55 0.0025690332815342266 56-57 0.0 58-59 0.004838832572889751 60-61 0.0 62-63 0.0 64-65 0.0021895170013075792 66-67 3.649195002179299E-4 68-69 0.0 70-71 0.0012042343507191688 72-73 0.0026639123515908884 74-75 0.0049410100329507715 76-77 0.0020873395412465594 78-79 1.6056458009588916E-4 80-81 9.633874805753351E-4 82-83 8.977019705361077E-4 84-85 0.0 86-87 0.0 88-89 7.298390004358598E-6 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6850826.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.655774785683285 #Duplication Level Percentage of deduplicated Percentage of total 1 80.78250076983917 43.344476679307775 2 10.530810243549633 11.300775654773314 3 3.2111037390002357 5.168827770997865 4 1.4931478290408242 3.204640145469856 5 0.8296469135355916 2.225767397215148 6 0.5265337132647964 1.6950944601603273 7 0.3890500681127915 1.461234798451028 8 0.2935751606433097 1.2601602161718575 9 0.2369109913274407 1.1440478515426311 >10 1.4398565938893932 14.63855463990835 >50 0.17591560805638431 6.625212383760413 >100 0.09042903477635182 7.286833363293625 >500 3.0067557482898745E-4 0.11905916602365095 >1k 1.3666211903465717E-4 0.10261396967344624 >5k 5.4664846860992414E-5 0.15779995749845938 >10k+ 2.7332423430496207E-5 0.2649015457522929 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 18063 0.26366163729745873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.379034002615159E-5 0.0 0.0 0.0 1.4596780008717196E-5 2 4.379034002615159E-5 0.0 0.0 0.0 2.9193560017434393E-5 3 8.758068005230318E-5 0.0 0.0 0.0 4.379034002615159E-5 4 1.3137102007845477E-4 0.0 0.0 0.0 5.8387120034868786E-5 5 4.233066202527987E-4 0.0 0.0 0.0 5.8387120034868786E-5 6 0.0015618554609327401 0.0 0.0 1.4596780008717196E-5 5.8387120034868786E-5 7 0.002320888021386034 0.0 0.0 1.4596780008717196E-5 5.8387120034868786E-5 8 0.0042914533225628555 0.0 0.0 1.4596780008717196E-5 5.8387120034868786E-5 9 0.00869968088519545 0.0 0.0 1.4596780008717196E-5 7.298390004358598E-5 10-11 0.022537428333459352 0.0 0.0 2.9193560017434393E-5 8.758068005230318E-5 12-13 0.036543038751823506 0.0 0.0 3.649195002179299E-5 8.758068005230318E-5 14-15 0.048008809448670864 0.0 0.0 4.379034002615159E-5 8.758068005230318E-5 16-17 0.058795829875112865 0.0 0.0 4.379034002615159E-5 9.487907005666178E-5 18-19 0.06624018767955864 0.0 0.0 5.108873003051019E-5 1.0217746006102037E-4 20-21 0.07529748967496766 0.0 0.0 7.298390004358598E-5 1.0217746006102037E-4 22-23 0.08456644498050309 0.0 0.0 7.298390004358598E-5 1.0217746006102037E-4 24-25 0.09372592443597313 0.0 0.0 7.298390004358598E-5 1.0217746006102037E-4 26-27 0.10394367044207516 0.0 0.0 7.298390004358598E-5 1.0947585006537897E-4 28-29 0.111409923416534 0.0 0.0 7.298390004358598E-5 1.2407263007409617E-4 30-31 0.11875940215092312 0.0 0.0 7.298390004358598E-5 1.3137102007845477E-4 32-33 0.12646650199552578 0.0 0.0 7.298390004358598E-5 1.4596780008717196E-4 34-35 0.13423198896016333 7.298390004358598E-6 0.0 7.298390004358598E-5 1.5326619009153056E-4 36-37 0.14301195213540674 1.4596780008717196E-5 0.0 7.298390004358598E-5 1.6056458009588916E-4 38-39 0.15201086701078087 1.4596780008717196E-5 0.0 8.028229004794458E-5 1.8245975010896496E-4 40-41 0.16171772571657783 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.0435492012204075E-4 42-43 0.1728477704732247 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.0435492012204075E-4 44-45 0.18538640450071275 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.0435492012204075E-4 46-47 0.20010725713950406 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.1165331012639935E-4 48-49 0.21773286900003008 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.3354848013947514E-4 50-51 0.23821944974226467 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.3354848013947514E-4 52-53 0.26133345088606835 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.3354848013947514E-4 54-55 0.28851265526229974 1.4596780008717196E-5 0.0 8.758068005230318E-5 2.5544365015255094E-4 56-57 0.3197497644809546 1.4596780008717196E-5 0.0 1.0217746006102037E-4 2.9193560017434393E-4 58-59 0.35535131092221584 1.4596780008717196E-5 0.0 1.0217746006102037E-4 2.9193560017434393E-4 60-61 0.3964339482567504 1.4596780008717196E-5 0.0 1.0217746006102037E-4 3.138307701874197E-4 62-63 0.44126795805352526 1.4596780008717196E-5 0.0 1.0947585006537897E-4 3.649195002179299E-4 64-65 0.4916925345936388 1.4596780008717196E-5 0.0 1.1677424006973757E-4 4.233066202527987E-4 66-67 0.5502767111586253 1.4596780008717196E-5 0.0 1.1677424006973757E-4 4.670969602789503E-4 68-69 0.6148601643071945 1.4596780008717196E-5 0.0 1.1677424006973757E-4 4.962905202963847E-4 70-71 0.6870923301803316 1.4596780008717196E-5 0.0 1.1677424006973757E-4 5.108873003051019E-4 72-73 0.7671191765781236 1.4596780008717196E-5 0.0 1.3137102007845477E-4 5.108873003051019E-4 74-75 0.8550574777406403 1.4596780008717196E-5 0.0 1.3866941008281337E-4 5.181856903094604E-4 76-77 0.952680742438941 1.4596780008717196E-5 0.0 1.4596780008717196E-4 5.254840803138191E-4 78-79 1.0583030425820186 1.4596780008717196E-5 0.0 1.4596780008717196E-4 5.400808603225363E-4 80-81 1.1775003481332031 1.4596780008717196E-5 0.0 1.4596780008717196E-4 5.546776403312535E-4 82-83 1.3082437066712833 1.4596780008717196E-5 0.0 1.6056458009588916E-4 5.692744203399707E-4 84-85 1.451949005857104 1.4596780008717196E-5 0.0 1.6056458009588916E-4 5.98467980357405E-4 86-87 1.606039914019127 1.4596780008717196E-5 0.0 1.6786297010024776E-4 6.276615403748394E-4 88-89 1.771888528478172 1.4596780008717196E-5 0.0 1.7516136010460636E-4 6.495567103879153E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 24955 0.0 89.36349 1 TATCTCG 10110 0.0 83.3823 2 ATATCTC 10495 0.0 80.44753 1 TCGACGG 27860 0.0 80.21679 2 ATCTCGA 10840 0.0 77.94242 3 CGACGGC 28725 0.0 77.78467 3 CGGCGCG 29590 0.0 75.49819 6 GACGGCG 30065 0.0 74.49057 4 ACGGCGC 30090 0.0 74.38786 5 TCTCGAG 7790 0.0 74.29762 4 AGGGCGC 8005 0.0 71.7291 9 TCGAGGG 8190 0.0 70.17913 6 CTCGAGG 8245 0.0 70.13117 5 CTCGACG 3790 0.0 69.82761 5 CGAGGGC 8350 0.0 68.833885 7 CGCGCCG 36695 0.0 60.98582 9 TCTCGAC 4415 0.0 59.950058 4 GAGGGCG 9620 0.0 59.93936 8 GCGCGCC 37460 0.0 59.783646 8 GGCGCGC 37570 0.0 59.701904 7 >>END_MODULE