FastQCFastQC Report
Tue 31 May 2016
SRR2048254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6850826
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT246770.3602047402751143No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT243530.3554753835522899No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84030.1226567424132506No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.11087714094621584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG66600.072.0593641
ATATCTC55400.067.7581
TCGACGG76550.064.244862
TATCTCG62200.059.8920172
CGACGGC85850.057.174623
GACGGCG101600.048.49854
ACGGCGC102550.048.0776525
CGGCGCG102700.047.961186
ATCTCGA103800.036.9876981
TCTCGAG97450.034.9119762
TCGAGGG109300.031.170394
GGTCGAC6650.030.7248691
CTCGAGG111400.030.625453
CGAGGGC112700.030.260925
GGGCGCG115350.029.5657188
AGGGCGC117700.029.0561187
CGCGCCG213350.027.0943989
GAGGGCG126800.027.008326
GCGCGCC221450.026.1462618
GGCGCGC221550.026.070147
Produced by FastQC (version 0.11.5)