##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048254_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6850826 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.282044530104834 33.0 31.0 34.0 30.0 34.0 2 32.41332548805064 34.0 31.0 34.0 31.0 34.0 3 32.49254615428855 34.0 31.0 34.0 31.0 34.0 4 36.052887637198786 37.0 35.0 37.0 35.0 37.0 5 35.91540071226448 37.0 35.0 37.0 35.0 37.0 6 35.9063984401297 37.0 35.0 37.0 35.0 37.0 7 35.88375372546318 37.0 35.0 37.0 35.0 37.0 8 35.854456090404284 37.0 35.0 37.0 35.0 37.0 9 37.45974718960896 39.0 37.0 39.0 34.0 39.0 10-11 37.45437791005055 39.0 37.0 39.0 34.0 39.0 12-13 37.45744775885419 39.0 37.0 39.0 34.0 39.0 14-15 38.796716921434 40.0 38.0 41.0 35.0 41.0 16-17 38.80275750690501 40.0 38.0 41.0 34.5 41.0 18-19 38.83695382133483 40.0 38.0 41.0 35.0 41.0 20-21 38.85494420672777 40.0 38.0 41.0 35.0 41.0 22-23 38.83318916580278 40.0 38.0 41.0 35.0 41.0 24-25 38.817184745313924 40.0 38.0 41.0 35.0 41.0 26-27 38.73130634174623 40.0 38.0 41.0 34.0 41.0 28-29 38.64697322629418 40.0 38.0 41.0 34.0 41.0 30-31 38.51921126007287 40.0 38.0 41.0 34.0 41.0 32-33 38.42427248918598 40.0 38.0 41.0 34.0 41.0 34-35 38.374654603693045 40.0 38.0 41.0 34.0 41.0 36-37 38.291428872956345 40.0 38.0 41.0 34.0 41.0 38-39 38.19896688370132 40.0 37.5 41.0 33.5 41.0 40-41 38.05540937691309 40.0 37.0 41.0 33.0 41.0 42-43 37.98200771702565 40.0 37.0 41.0 33.0 41.0 44-45 37.87087827073699 40.0 37.0 41.0 33.0 41.0 46-47 37.76399283823586 40.0 37.0 41.0 33.0 41.0 48-49 37.65859576640831 40.0 36.5 41.0 33.0 41.0 50-51 37.56197413275421 40.0 36.0 41.0 32.0 41.0 52-53 37.53320198177563 39.0 36.0 41.0 32.0 41.0 54-55 37.35898634704779 39.0 36.0 41.0 32.0 41.0 56-57 37.12466533232635 39.0 35.0 41.0 32.0 41.0 58-59 36.8861392042361 39.0 35.0 41.0 31.0 41.0 60-61 36.6222413180542 38.5 35.0 40.5 31.0 41.0 62-63 36.310427457944485 38.0 35.0 40.0 31.0 41.0 64-65 35.9968225729277 37.0 35.0 40.0 31.0 41.0 66-67 35.65843206352052 37.0 35.0 39.5 30.5 41.0 68-69 35.303322767210844 36.0 34.0 39.0 30.0 41.0 70-71 34.95051837544845 36.0 34.0 39.0 30.0 41.0 72-73 34.58401257016307 35.0 34.0 38.5 29.5 40.0 74-75 34.25412993995177 35.0 34.0 37.0 29.0 39.5 76-77 34.014711218763985 35.0 34.0 37.0 29.5 39.0 78-79 33.86892908971852 35.0 34.0 36.0 30.0 39.0 80-81 33.631085507061485 35.0 34.0 36.0 30.0 37.5 82-83 33.401801841121056 35.0 34.0 36.0 29.0 37.0 84-85 33.204271718475994 35.0 34.0 35.0 29.0 37.0 86-87 33.010909195475115 35.0 34.0 35.0 29.0 36.0 88-89 32.87887686827837 35.0 34.0 35.0 29.0 36.0 90-91 32.75053263650251 35.0 33.0 35.0 29.0 36.0 92-93 32.621769769076025 35.0 33.0 35.0 29.0 36.0 94-95 32.514543501761686 35.0 33.0 35.0 29.0 35.0 96-97 32.405616417640736 35.0 33.0 35.0 29.0 35.0 98-99 32.262189187114075 35.0 33.0 35.0 29.0 35.0 100-101 31.811147736054018 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3463029691514947 1101 2 0.3678149139974778 1101 3 0.33353968599716666 1101 4 0.23824361882508072 1101 5 0.25161842537810486 1101 6 0.3058292997408145 1101 7 0.27399784314090425 1101 8 0.32681417451950523 1101 9 0.42083669150789405 1101 10-11 0.39645565987347453 1101 12-13 0.40137367968986126 1101 14-15 0.49682116262867737 1101 16-17 0.4811974397468717 1101 18-19 0.5085604123665703 1101 20-21 0.4758804609050955 1101 22-23 0.571714120863561 1101 24-25 0.5059661464013558 1101 26-27 0.5408986137079594 1101 28-29 0.5188994391132908 1101 30-31 0.5422548245379488 1101 32-33 0.5327891338216517 1101 34-35 0.5260653779527189 1101 36-37 0.5626536220905649 1101 38-39 0.49472949246597864 1101 40-41 0.4872040752954021 1101 42-43 0.41017962412760767 1101 44-45 0.472903944830513 1101 46-47 0.4551183816110651 1101 48-49 0.47929531213625154 1101 50-51 0.5113002572042831 1101 52-53 0.4608703633471123 1101 54-55 0.42715598384724274 1101 56-57 0.3922999739710491 1101 58-59 0.3630522707513322 1101 60-61 0.3897635418861043 1101 62-63 0.40688973315943855 1101 64-65 0.3400170607572335 1101 66-67 0.34691675242203246 1101 68-69 0.32977244449764953 1101 70-71 0.2807843474168834 1101 72-73 0.2904951475684072 1101 74-75 0.26126488355135535 1101 76-77 0.30475647196612243 1101 78-79 0.29793808796080157 1101 80-81 0.3235948901150323 1101 82-83 0.3070134858135489 1101 84-85 0.25596457340348877 1101 86-87 0.30527666523764196 1101 88-89 0.29347778011192105 1101 90-91 0.2801150441953624 1101 92-93 0.3548118326784575 1101 94-95 0.3253308429026802 1101 96-97 0.4100673029785469 1101 98-99 0.4392245542830011 1101 100-101 0.4420054754160425 1102 1 0.3695185783007986 1102 2 0.37967210597440015 1102 3 0.33854927260006207 1102 4 0.24223529192180138 1102 5 0.2559564496549527 1102 6 0.27045757911287893 1102 7 0.27094640192852637 1102 8 0.2890364094833089 1102 9 0.3630278951522712 1102 10-11 0.38213572182240796 1102 12-13 0.37492210273304494 1102 14-15 0.51215374615537 1102 16-17 0.47354038158956513 1102 18-19 0.503261574019696 1102 20-21 0.47579807395211304 1102 22-23 0.5438867972422514 1102 24-25 0.4731210236764696 1102 26-27 0.5029737173322033 1102 28-29 0.484496701398335 1102 30-31 0.5109145588838757 1102 32-33 0.5198776129560727 1102 34-35 0.4921209200891852 1102 36-37 0.5176021087556464 1102 38-39 0.5329708199367218 1102 40-41 0.5566961313042071 1102 42-43 0.48545152944998193 1102 44-45 0.5476663889106916 1102 46-47 0.4980577348500077 1102 48-49 0.4957138763926352 1102 50-51 0.4798537626357273 1102 52-53 0.4773347148414473 1102 54-55 0.5217089246583555 1102 56-57 0.5006066220131444 1102 58-59 0.4489164780180417 1102 60-61 0.3852653326138693 1102 62-63 0.41784434075213994 1102 64-65 0.45938975997763976 1102 66-67 0.47206303849978326 1102 68-69 0.3866117680037462 1102 70-71 0.35943602904936256 1102 72-73 0.38221998061719376 1102 74-75 0.36156371767798845 1102 76-77 0.3517199796393484 1102 78-79 0.3840935455245216 1102 80-81 0.37312744553972976 1102 82-83 0.3370573211637051 1102 84-85 0.28518117757996464 1102 86-87 0.3259429696229148 1102 88-89 0.29365366733097176 1102 90-91 0.274440768930603 1102 92-93 0.3153140265609409 1102 94-95 0.3172757670887876 1102 96-97 0.3381685573975233 1102 98-99 0.400925128099594 1102 100-101 0.3710592489398046 1103 1 0.2825793354541162 1103 2 0.3111501845028002 1103 3 0.2883146349867829 1103 4 0.18590451517113138 1103 5 0.2124504553231219 1103 6 0.24406544205434955 1103 7 0.21453163828541477 1103 8 0.2023006867803545 1103 9 0.3274039162304021 1103 10-11 0.31216262001568396 1103 12-13 0.30748593218091713 1103 14-15 0.45784774214676816 1103 16-17 0.4223651965791717 1103 18-19 0.4280555295932871 1103 20-21 0.4615136473295678 1103 22-23 0.4700402333693319 1103 24-25 0.47483931652513434 1103 26-27 0.4975866727926004 1103 28-29 0.4371228608149025 1103 30-31 0.462170979084803 1103 32-33 0.474320826098662 1103 34-35 0.45289402533813927 1103 36-37 0.5197591436138538 1103 38-39 0.467775713643519 1103 40-41 0.4522757077890418 1103 42-43 0.5032215585266755 1103 44-45 0.48603869931925203 1103 46-47 0.48202865080599366 1103 48-49 0.46850850420143075 1103 50-51 0.4434373870790722 1103 52-53 0.5019145543768886 1103 54-55 0.45530017895805486 1103 56-57 0.45248063067899125 1103 58-59 0.38019101278700873 1103 60-61 0.3674544530910637 1103 62-63 0.41181423584844623 1103 64-65 0.4119137059008082 1103 66-67 0.40283074718233536 1103 68-69 0.3259066023035473 1103 70-71 0.3588105671084634 1103 72-73 0.3776785200026609 1103 74-75 0.3007233331753909 1103 76-77 0.2732843364447959 1103 78-79 0.23172604325455382 1103 80-81 0.2481899149827811 1103 82-83 0.16246799331040052 1103 84-85 0.20947675204317306 1103 86-87 0.2110049283054991 1103 88-89 0.20071741935239373 1103 90-91 0.25730348487815746 1103 92-93 0.25312261120402013 1103 94-95 0.2443944243013263 1103 96-97 0.247864393071211 1103 98-99 0.310322310124441 1103 100-101 0.33737090764390487 1104 1 0.23808880265946897 1104 2 0.24266831608923667 1104 3 0.19449009573238385 1104 4 0.13928365755369754 1104 5 0.13850642653719092 1104 6 0.1661980924174813 1104 7 0.1415677456105584 1104 8 0.1727335702675532 1104 9 0.24667712602600034 1104 10-11 0.1712069684905373 1104 12-13 0.21894043037198685 1104 14-15 0.25809845166624257 1104 16-17 0.22585957920752264 1104 18-19 0.2666847202309057 1104 20-21 0.2969771994103496 1104 22-23 0.34207364518181294 1104 24-25 0.34278256020598974 1104 26-27 0.28606016512862453 1104 28-29 0.2757033213652349 1104 30-31 0.32261107145188817 1104 32-33 0.3128859195610829 1104 34-35 0.23936707518319622 1104 36-37 0.31562130430352653 1104 38-39 0.3154751434396985 1104 40-41 0.3388868086892529 1104 42-43 0.3083143627318776 1104 44-45 0.3407030126630701 1104 46-47 0.39023951739593343 1104 48-49 0.33973245511957373 1104 50-51 0.32915513071756664 1104 52-53 0.3725478270886242 1104 54-55 0.3559696794411735 1104 56-57 0.3161295112050837 1104 58-59 0.23929081099113603 1104 60-61 0.19358886840021228 1104 62-63 0.19269543208012152 1104 64-65 0.1618763812182067 1104 66-67 0.08069202377559037 1104 68-69 0.13218596061306442 1104 70-71 0.11536512358193107 1104 72-73 0.13428045064389238 1104 74-75 0.06872985679014931 1104 76-77 0.1363977302233721 1104 78-79 0.08677700581259273 1104 80-81 0.05507754155671307 1104 82-83 0.10260754546992246 1104 84-85 0.12119881374112396 1104 86-87 0.13106182268860778 1104 88-89 0.15900504574553764 1104 90-91 0.16575827401081256 1104 92-93 0.19597953215946973 1104 94-95 0.19652595469337797 1104 96-97 0.16839718753445254 1104 98-99 0.17958450376573154 1104 100-101 0.21828548624523947 1105 1 0.16226479438262942 1105 2 0.2057200735560869 1105 3 0.16971544899547553 1105 4 0.12593464178958413 1105 5 0.16123707534330833 1105 6 0.17234843683197454 1105 7 0.14850684005908477 1105 8 0.16090841848013326 1105 9 0.16627401132100061 1105 10-11 0.19237245080697107 1105 12-13 0.1726473649468474 1105 14-15 0.273733181716068 1105 16-17 0.19664947275213507 1105 18-19 0.23295627676660757 1105 20-21 0.2047455568390859 1105 22-23 0.2089242809192342 1105 24-25 0.22445058819477737 1105 26-27 0.21162184663369743 1105 28-29 0.15745923630983327 1105 30-31 0.19712976240453628 1105 32-33 0.2090780293381016 1105 34-35 0.18001318588591175 1105 36-37 0.17827266970961375 1105 38-39 0.19541554475745926 1105 40-41 0.25751285350397524 1105 42-43 0.15800117696586824 1105 44-45 0.2547180416592312 1105 46-47 0.15409979129393747 1105 48-49 0.22944363701943615 1105 50-51 0.23712071649447353 1105 52-53 0.24298140818258673 1105 54-55 0.2007475933511671 1105 56-57 0.19634928304749621 1105 58-59 0.14597860537703866 1105 60-61 0.13612013796921474 1105 62-63 -0.0021201087731554935 1105 64-65 0.0027336329811191717 1105 66-67 0.12693444801801945 1105 68-69 0.10621811504144318 1105 70-71 0.10994022772307233 1105 72-73 0.041817247180688355 1105 74-75 0.02539690462700861 1105 76-77 0.13378391008124169 1105 78-79 0.11470466515534383 1105 80-81 0.10729853858903482 1105 82-83 0.08693415387200076 1105 84-85 0.07219998447815357 1105 86-87 0.08583695950148496 1105 88-89 0.055744407013200714 1105 90-91 0.03566360767086252 1105 92-93 0.05079602593822585 1105 94-95 -0.09430937978346776 1105 96-97 -0.029406700401509056 1105 98-99 -0.002142877867306936 1105 100-101 -0.04215805972661357 1106 1 0.1274914054981906 1106 2 0.14802234634405664 1106 3 0.10918920911085195 1106 4 0.10856776413555025 1106 5 0.0834346022508754 1106 6 0.11709292845925034 1106 7 0.12859865962482786 1106 8 0.1439824777168397 1106 9 0.18686109034778298 1106 10-11 0.16583934715608706 1106 12-13 0.1759768524344949 1106 14-15 0.26427704839341715 1106 16-17 0.2218861257981004 1106 18-19 0.23975872315178748 1106 20-21 0.23554549274165737 1106 22-23 0.2649262305493423 1106 24-25 0.22070235755085577 1106 26-27 0.24264273171235118 1106 28-29 0.23834695898163005 1106 30-31 0.23990146873148888 1106 32-33 0.24227301905580845 1106 34-35 0.18885871108421526 1106 36-37 0.1946208789877133 1106 38-39 0.22103910590373488 1106 40-41 0.20428202264106687 1106 42-43 0.18330900486427026 1106 44-45 0.23267958320237625 1106 46-47 0.2000280555890015 1106 48-49 0.22217701842583892 1106 50-51 0.1853164807227614 1106 52-53 0.09527996866117405 1106 54-55 0.14875480431182808 1106 56-57 0.1771744306051133 1106 58-59 0.17942378872238862 1106 60-61 0.121160679592478 1106 62-63 0.1573946202816714 1106 64-65 0.07854668214918092 1106 66-67 0.11196379929862843 1106 68-69 0.11199332001967832 1106 70-71 0.1452596999875766 1106 72-73 0.13078786919281526 1106 74-75 0.08940064364364986 1106 76-77 0.13072996141260518 1106 78-79 0.146524097358963 1106 80-81 0.09783281735943206 1106 82-83 0.12243360637313572 1106 84-85 0.1693777213047909 1106 86-87 0.1817931595922886 1106 88-89 0.1942407881792363 1106 90-91 0.12282540026226485 1106 92-93 0.1387247442567343 1106 94-95 0.12742758039600943 1106 96-97 0.1917204261642027 1106 98-99 0.23756961846044078 1106 100-101 0.25512920358028524 1107 1 0.006259241298103291 1107 2 0.06847833916065582 1107 3 0.07737137340883038 1107 4 0.056503994693500204 1107 5 0.08295936720372765 1107 6 0.09474095364623025 1107 7 0.10991934528195202 1107 8 0.06650924335579589 1107 9 0.1576052959435401 1107 10-11 0.05036135811627673 1107 12-13 0.09715056605745787 1107 14-15 0.10219993733991828 1107 16-17 0.022905804211980296 1107 18-19 0.018514941330657564 1107 20-21 0.01786461119731797 1107 22-23 0.12576119275728104 1107 24-25 0.11976625139750041 1107 26-27 0.03808478788152314 1107 28-29 0.03654390737044366 1107 30-31 0.11604058957151864 1107 32-33 0.09225663109298665 1107 34-35 0.027316206386501563 1107 36-37 0.024455948455582188 1107 38-39 0.030146220158101755 1107 40-41 0.09061204075883467 1107 42-43 0.07845325465513753 1107 44-45 0.0659140304991439 1107 46-47 0.08394414318697585 1107 48-49 0.06748315557239692 1107 50-51 0.1065564314541021 1107 52-53 0.20146286869773178 1107 54-55 0.07865027958841608 1107 56-57 0.14992355555298786 1107 58-59 0.03341122031375221 1107 60-61 0.05846959142169794 1107 62-63 -0.11654181971623956 1107 64-65 -0.05243440173913427 1107 66-67 -0.08427208089980809 1107 68-69 -0.012317447612716137 1107 70-71 0.0502104284158591 1107 72-73 -0.03180725917435723 1107 74-75 0.03335576867078771 1107 76-77 -0.020083018065612634 1107 78-79 -0.0517070583226058 1107 80-81 -0.05975798965486945 1107 82-83 -0.0016226371999366052 1107 84-85 0.05927930462964781 1107 86-87 -0.06320826039797112 1107 88-89 -0.016213594415511068 1107 90-91 0.00855995907867424 1107 92-93 0.03245285340173609 1107 94-95 0.07281214111473133 1107 96-97 0.09134138904088474 1107 98-99 0.03158409098419668 1107 100-101 -0.055443315748068045 1108 1 -0.04482054059675278 1108 2 0.028105803049840006 1108 3 -3.927427689234264E-4 1108 4 0.014870276009460781 1108 5 -0.019422756652609507 1108 6 -0.05789312047101447 1108 7 -0.015536726493969866 1108 8 -0.03712525390296406 1108 9 -0.20496936200052573 1108 10-11 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-0.09019793224724992 2214 80-81 -0.13205643976594672 2214 82-83 -0.12988835695050938 2214 84-85 -0.2420402752621058 2214 86-87 -0.1870851184205975 2214 88-89 -0.19121879645000206 2214 90-91 -0.2004987299914731 2214 92-93 -0.23183817319596756 2214 94-95 -0.20035483432892676 2214 96-97 -0.14611882161363354 2214 98-99 -0.2424545661789992 2214 100-101 -0.42873814630669926 2215 1 -0.6334093568268315 2215 2 -0.9881456385601979 2215 3 -0.6334271912041132 2215 4 -0.34612566843928505 2215 5 -0.3616584664725053 2215 6 -0.3756498276218707 2215 7 -0.36842623294232624 2215 8 -0.4689206302236286 2215 9 -0.4764501453120431 2215 10-11 -0.5731777173240715 2215 12-13 -0.45462262095184514 2215 14-15 -0.6341915389694748 2215 16-17 -0.5980605825684009 2215 18-19 -0.5836114637692376 2215 20-21 -0.5195818105543353 2215 22-23 -0.5534292281381425 2215 24-25 -0.6000439339876777 2215 26-27 -0.6058491372040251 2215 28-29 -0.567618646323858 2215 30-31 -0.6093836338423415 2215 32-33 -0.6101550762047978 2215 34-35 -0.7496886255903874 2215 36-37 -0.6541247703598145 2215 38-39 -0.6274482148694034 2215 40-41 -0.6662181368420477 2215 42-43 -0.6554099305295225 2215 44-45 -0.5978026221871744 2215 46-47 -0.5894899689837487 2215 48-49 -0.6510750743375482 2215 50-51 -0.6198472843733072 2215 52-53 -0.6913428135334385 2215 54-55 -0.7205473410234902 2215 56-57 -0.5977967197727665 2215 58-59 -0.5654365155688339 2215 60-61 -0.48719052479246727 2215 62-63 -0.5228913420956403 2215 64-65 -0.47926940422465236 2215 66-67 -0.5142606924527513 2215 68-69 -0.5181952703582766 2215 70-71 -0.47183209031575757 2215 72-73 -0.4735845317541134 2215 74-75 -0.5256676359956174 2215 76-77 -0.3900957948759114 2215 78-79 -0.3595306098202471 2215 80-81 -0.360098684537121 2215 82-83 -0.4644686013075159 2215 84-85 -0.4672611284408248 2215 86-87 -0.4087129916862864 2215 88-89 -0.37821372293581845 2215 90-91 -0.3820262719256249 2215 92-93 -0.4000538493193915 2215 94-95 -0.415673844475954 2215 96-97 -0.45814853791100063 2215 98-99 -0.541366763321264 2215 100-101 -0.5468265704110671 2216 1 -0.4945119449187949 2216 2 -0.5048801364827149 2216 3 -0.5018571440169985 2216 4 -0.31864566289849705 2216 5 -0.343246954237884 2216 6 -0.36246197019509907 2216 7 -0.370010663906605 2216 8 -0.46474734638186277 2216 9 -0.5346248580726538 2216 10-11 -0.6838807777706108 2216 12-13 -0.7143048578173179 2216 14-15 -0.9727929347192514 2216 16-17 -1.2699679622259197 2216 18-19 -1.272494159788927 2216 20-21 -1.6493911177809295 2216 22-23 -1.4491575595127912 2216 24-25 -1.5001028816198385 2216 26-27 -1.2869628741421266 2216 28-29 -1.3290315733941398 2216 30-31 -1.3704225869102018 2216 32-33 -1.0889106514161 2216 34-35 -0.6814063907848436 2216 36-37 -1.0464039716029987 2216 38-39 -1.341966101894002 2216 40-41 -1.34813142513579 2216 42-43 -0.7048110184668843 2216 44-45 -1.1904152714611698 2216 46-47 -0.8472604604010954 2216 48-49 -0.8008842344262703 2216 50-51 -0.9578699196418725 2216 52-53 -0.9657754741034097 2216 54-55 -1.1826148466142001 2216 56-57 -1.27115620300777 2216 58-59 -0.9074273539246462 2216 60-61 -0.9591153339809395 2216 62-63 -0.8530840023124853 2216 64-65 -0.9886820109547756 2216 66-67 -0.753408881931847 2216 68-69 -0.7263726168866498 2216 70-71 -0.6545321794182399 2216 72-73 -0.5717686907655875 2216 74-75 -0.5679873653123977 2216 76-77 -0.5744566458624618 2216 78-79 -0.46150718841509075 2216 80-81 -0.47296803052823577 2216 82-83 -0.6704880197219936 2216 84-85 -0.5034325970217353 2216 86-87 -0.6187050987908691 2216 88-89 -0.5746880180561149 2216 90-91 -0.5555274573313156 2216 92-93 -0.5357404650076916 2216 94-95 -0.6291785317627969 2216 96-97 -0.6141256878211507 2216 98-99 -0.8603506712735012 2216 100-101 -0.6445444915716543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 11.0 12 47.0 13 267.0 14 965.0 15 2199.0 16 4003.0 17 6381.0 18 10983.0 19 22010.0 20 24512.0 21 22547.0 22 22299.0 23 23349.0 24 26381.0 25 31995.0 26 41057.0 27 54461.0 28 70838.0 29 92117.0 30 120540.0 31 157405.0 32 205970.0 33 270314.0 34 365866.0 35 523644.0 36 874143.0 37 1635969.0 38 1825798.0 39 414126.0 40 628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.26748949688694 39.507717755494006 14.098314568199513 20.126478179419532 2 24.14758453944094 28.419901483412364 29.57728017030355 17.855233806843145 3 22.526801877613007 27.647352304670996 25.0210996455026 24.80474617221339 4 23.667210819703588 26.76250165925545 26.01201439671516 23.5582731243258 5 23.512011544301373 27.486714740675065 25.419460368720504 23.58181334630306 6 23.465535396753616 26.179091397154153 26.242339244931923 24.113033961160305 7 24.27978757597989 27.36205823940062 24.794148909927067 23.564005274692423 8 24.061405150269472 25.9060586270911 26.300667977846757 23.731868244792672 9 23.1753222166203 25.803589231429903 26.530698634004136 24.49038991794566 10-11 24.243025585527935 26.435133515287063 25.60929149273387 23.71254940645113 12-13 23.90095734441365 26.55252811850717 25.546656709716466 23.999857827362714 14-15 23.581922822153125 26.45359844199809 25.837614325630227 24.126864410218563 16-17 23.773724511467666 26.823933347599255 25.667072846398376 23.735269294534703 18-19 23.226857843627748 26.651233490037075 26.425720520833586 23.69618814550159 20-21 22.75534366220949 26.898114183603557 26.56894219762697 23.777599956559982 22-23 22.54739392700879 26.835629285524004 27.19869504767674 23.418281739790466 24-25 22.476012381572673 26.353157998758103 28.07197263512458 23.09885698454464 26-27 22.426445124346785 26.138817970781748 28.687118572147675 22.747618332723796 28-29 22.478985877508347 26.367716107125734 28.567631314226443 22.585666701139477 30-31 22.51686074551032 26.30923666992467 28.486164583997486 22.687738000567524 32-33 22.46653997143555 26.249990238399807 28.591643033863008 22.691826756301634 34-35 22.546507530624773 26.49433805500242 28.438672942503572 22.520481471869232 36-37 22.537906018618195 26.44381516094777 28.28249882313444 22.735779997299595 38-39 22.527354189265832 26.37030039231044 28.419228955580454 22.683116462843266 40-41 22.70188192868953 26.627792960475155 27.87922025364126 22.79110485719405 42-43 22.755095211602058 26.68690220199204 27.681046041280172 22.876956545125733 44-45 22.700679306931185 26.677817179467645 27.795773227266977 22.825730286334192 46-47 22.78475026124614 26.92041637649455 27.54991045596154 22.744922906297774 48-49 22.80943203053179 26.94371452435079 27.36954638754509 22.877307057572327 50-51 22.73413853181869 26.76908123061545 27.615783287713462 22.8809969498524 52-53 22.767471431192625 26.96703174152986 27.47404258896957 22.791454238307942 54-55 22.807032268819146 26.903956085469034 27.3597885899408 22.929223055771022 56-57 22.816349629285362 26.846260200600746 27.45415805577783 22.88323211433606 58-59 22.92886059416011 27.016546811106412 27.23997503337593 22.81461756135755 60-61 22.848443765010114 26.975102312913375 27.170999228264975 23.005454693811537 62-63 22.847234741026785 26.890474914991742 27.326102552911664 22.93618779106981 64-65 22.979245162526343 27.089713811318 27.07094231523633 22.860098710919324 66-67 22.95253647082728 27.00497955134701 26.989499648071224 23.05298432975449 68-69 22.9175988891788 26.99232752439044 27.012208450488355 23.077865135942403 70-71 23.014871916025555 27.149623507391148 26.86121917029088 22.97428540629242 72-73 22.967331511459502 27.11078320236608 26.73793013451525 23.183955151659173 74-75 22.952247654954583 27.053638541691676 26.894868148830202 23.099245654523536 76-77 23.02718142250038 27.22831495732896 26.72166476761339 23.02283885255727 78-79 23.0062920191396 27.21080968172941 26.633200120598676 23.14969817853231 80-81 22.94506243593379 27.160424897886347 26.775266765413935 23.119245900765932 82-83 23.000170348276832 27.325374404930553 26.605569001926966 23.06888624486565 84-85 23.012349167822972 27.255217108126818 26.62794238242221 23.104491341628002 86-87 23.01094787694214 27.192115228149135 26.72709101063142 23.069845884277314 88-89 23.06059596225435 27.337444968130015 26.54809954395127 23.053859525664365 90-91 23.067583920934297 27.32448294241475 26.451680849055993 23.15625228759496 92-93 22.966051218356732 27.238767762418593 26.61289975317501 23.182281266049664 94-95 23.0951056120824 27.39504696222032 26.42887149666332 23.080975929033958 96-97 23.06433160835042 27.378722972561203 26.387742731954006 23.169202687134373 98-99 23.015824881249713 27.287762620723587 26.556806913269064 23.139605584757632 100-101 23.13380424586212 27.43498554650446 26.272253580382372 23.15895662725105 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5939.0 1 4153.5 2 1663.5 3 783.5 4 535.5 5 411.5 6 363.5 7 346.0 8 343.5 9 351.0 10 355.5 11 351.0 12 405.0 13 728.5 14 997.5 15 1162.0 16 6486.5 17 7572.5 18 3667.0 19 4247.5 20 5208.5 21 6606.5 22 8742.0 23 11416.5 24 14266.0 25 18121.5 26 23348.0 27 29787.5 28 36992.5 29 47012.0 30 60589.5 31 73873.0 32 88978.5 33 110910.5 34 133737.0 35 156750.5 36 179209.0 37 194986.0 38 208255.5 39 224450.5 40 232397.0 41 228955.0 42 226482.0 43 230434.0 44 239397.5 45 249803.0 46 257249.5 47 261830.0 48 263365.0 49 259364.5 50 251895.0 51 246659.0 52 247503.5 53 246301.5 54 234007.0 55 218679.0 56 203528.5 57 181246.5 58 160501.5 59 145427.0 60 128043.5 61 108548.5 62 88628.5 63 69497.5 64 53157.0 65 38568.5 66 27615.0 67 20090.0 68 14954.5 69 11662.5 70 8983.0 71 6806.5 72 5478.5 73 4076.0 74 2885.5 75 2135.5 76 1504.0 77 939.0 78 573.0 79 307.5 80 126.5 81 47.0 82 17.5 83 10.5 84 4.5 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.041965742525061946 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.298390004358598E-6 20-21 0.0 22-23 1.4596780008717196E-5 24-25 0.0 26-27 8.028229004794458E-5 28-29 2.4084687014383377E-4 30-31 2.9193560017434393E-5 32-33 3.649195002179299E-5 34-35 0.0 36-37 1.4596780008717196E-5 38-39 2.1895170013075795E-5 40-41 1.2407263007409617E-4 42-43 9.487907005666178E-5 44-45 2.9193560017434393E-4 46-47 1.0217746006102039E-4 48-49 0.0 50-51 1.4596780008717196E-4 52-53 0.0012407263007409618 54-55 9.341939205579006E-4 56-57 5.8387120034868786E-5 58-59 0.0011677424006973757 60-61 4.233066202527987E-4 62-63 3.065323801830611E-4 64-65 1.9705653011768215E-4 66-67 1.1677424006973757E-4 68-69 5.619760303356121E-4 70-71 4.0141145023972296E-4 72-73 5.8387120034868786E-5 74-75 7.663309504576529E-4 76-77 6.422583203835566E-4 78-79 6.568551003922738E-5 80-81 5.1088730030510194E-5 82-83 0.0022625009013511653 84-85 0.0 86-87 0.0 88-89 3.3572594020049557E-4 90-91 2.5544365015255094E-4 92-93 2.992339901787025E-4 94-95 0.0 96-97 4.88992130292026E-4 98-99 7.298390004358598E-6 100-101 0.008466132405055974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6850826.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.562844477316446 #Duplication Level Percentage of deduplicated Percentage of total 1 80.14186899916965 41.323427273256534 2 10.82065619285717 11.158876248296108 3 3.2637296981343704 5.048615605227045 4 1.518503524779612 3.1319344434587193 5 0.8399267755449681 2.1654506849879533 6 0.5717523867093816 1.7688707637258199 7 0.4122497974191812 1.487974053309132 8 0.31553855961527155 1.3016052540830954 9 0.2551581630488732 1.184101261056612 >10 1.5667573990886121 15.24791693471611 >50 0.19238907151090587 6.968187664118947 >100 0.10098750386385075 7.930555035690152 >500 1.7010097242222354E-4 0.05477357419700373 >1k 1.700876104463573E-4 0.19317829406981346 >5k 8.504380516246672E-5 0.3178613181963238 >10k+ 5.6695870108311145E-5 0.7166715916106758 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24677 0.3602047402751143 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 24353 0.3554753835522899 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8403 0.1226567424132506 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 7596 0.11087714094621584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.9193560017434393E-5 0.0 0.0 0.0 0.0 2 4.379034002615159E-5 0.0 0.0 0.0 0.0 3 7.298390004358598E-5 0.0 0.0 0.0 1.4596780008717196E-5 4 1.1677424006973757E-4 0.0 0.0 0.0 1.4596780008717196E-5 5 4.233066202527987E-4 0.0 0.0 0.0 1.4596780008717196E-5 6 0.0013720973208194165 0.0 0.0 1.4596780008717196E-5 1.4596780008717196E-5 7 0.0021457266612814278 0.0 0.0 1.4596780008717196E-5 1.4596780008717196E-5 8 0.003926533822344926 0.0 0.0 1.4596780008717196E-5 1.4596780008717196E-5 9 0.007940648324742155 0.0 0.0 1.4596780008717196E-5 4.379034002615159E-5 10-11 0.020180048362051527 0.0 0.0 1.4596780008717196E-5 4.379034002615159E-5 12-13 0.032156706359203986 7.298390004358598E-6 0.0 1.4596780008717196E-5 5.108873003051019E-5 14-15 0.041651911754874524 1.4596780008717196E-5 0.0 1.4596780008717196E-5 5.8387120034868786E-5 16-17 0.050526754000174576 1.4596780008717196E-5 0.0 1.4596780008717196E-5 7.298390004358598E-5 18-19 0.05699312754403629 1.4596780008717196E-5 0.0 1.4596780008717196E-5 9.487907005666178E-5 20-21 0.0642039368683426 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.0217746006102037E-4 22-23 0.07169938340281887 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.0217746006102037E-4 24-25 0.07935539451739104 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.0947585006537897E-4 26-27 0.08750769615225959 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.6786297010024776E-4 28-29 0.09427330368630002 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.7516136010460636E-4 30-31 0.10117758063042326 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.7516136010460636E-4 32-33 0.10835919639471212 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.8975814011332355E-4 34-35 0.11551891698898789 1.4596780008717196E-5 0.0 1.4596780008717196E-5 1.8975814011332355E-4 36-37 0.1238025896439349 1.4596780008717196E-5 0.0 2.9193560017434393E-5 2.0435492012204075E-4 38-39 0.13226142365898652 1.4596780008717196E-5 0.0 4.379034002615159E-5 2.0435492012204075E-4 40-41 0.14207045982484448 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.0435492012204075E-4 42-43 0.1537551822218226 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.1895170013075795E-4 44-45 0.16665143735952426 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.3354848013947514E-4 46-47 0.18167152398849423 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.3354848013947514E-4 48-49 0.19969854729925995 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.3354848013947514E-4 50-51 0.22038218457161224 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.3354848013947514E-4 52-53 0.24391219394566438 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.3354848013947514E-4 54-55 0.27133224519203963 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.6274204015690954E-4 56-57 0.3027883061108252 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.6274204015690954E-4 58-59 0.3384190461121038 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.6274204015690954E-4 60-61 0.3791221671664118 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.6274204015690954E-4 62-63 0.423963475353191 1.4596780008717196E-5 0.0 5.8387120034868786E-5 2.7004043016126813E-4 64-65 0.47430047121325225 1.4596780008717196E-5 0.0 5.8387120034868786E-5 3.065323801830611E-4 66-67 0.5324686395479903 1.4596780008717196E-5 0.0 5.8387120034868786E-5 3.211291601917783E-4 68-69 0.5967090683663546 1.4596780008717196E-5 0.0 5.8387120034868786E-5 3.576211102135713E-4 70-71 0.6689558310195004 1.4596780008717196E-5 0.0 5.8387120034868786E-5 3.722178902222885E-4 72-73 0.7489023951272444 1.4596780008717196E-5 0.0 6.568551003922738E-5 3.941130602353643E-4 74-75 0.8363517041594692 1.4596780008717196E-5 0.0 7.298390004358598E-5 4.087098402440815E-4 76-77 0.9335881541875388 1.4596780008717196E-5 0.0 7.298390004358598E-5 4.306050102571573E-4 78-79 1.0388893251704245 1.4596780008717196E-5 0.0 7.298390004358598E-5 4.525001802702331E-4 80-81 1.1573275981611562 1.4596780008717196E-5 0.0 7.298390004358598E-5 4.670969602789503E-4 82-83 1.287443295158861 1.4596780008717196E-5 0.0 7.298390004358598E-5 4.670969602789503E-4 84-85 1.429769198633858 1.4596780008717196E-5 0.0 9.487907005666178E-5 4.88992130292026E-4 86-87 1.5824442191350356 1.4596780008717196E-5 0.0 1.0947585006537897E-4 5.108873003051019E-4 88-89 1.747183478313418 1.4596780008717196E-5 0.0 1.1677424006973757E-4 5.400808603225363E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6660 0.0 72.059364 1 ATATCTC 5540 0.0 67.758 1 TCGACGG 7655 0.0 64.24486 2 TATCTCG 6220 0.0 59.892017 2 CGACGGC 8585 0.0 57.17462 3 GACGGCG 10160 0.0 48.4985 4 ACGGCGC 10255 0.0 48.077652 5 CGGCGCG 10270 0.0 47.96118 6 ATCTCGA 10380 0.0 36.987698 1 TCTCGAG 9745 0.0 34.911976 2 TCGAGGG 10930 0.0 31.17039 4 GGTCGAC 665 0.0 30.724869 1 CTCGAGG 11140 0.0 30.62545 3 CGAGGGC 11270 0.0 30.26092 5 GGGCGCG 11535 0.0 29.565718 8 AGGGCGC 11770 0.0 29.056118 7 CGCGCCG 21335 0.0 27.094398 9 GAGGGCG 12680 0.0 27.00832 6 GCGCGCC 22145 0.0 26.146261 8 GGCGCGC 22155 0.0 26.07014 7 >>END_MODULE