FastQCFastQC Report
Tue 31 May 2016
SRR2048253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253279
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT288990.39842669777351736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG302600.089.738941
TATCTCG158500.085.843392
ATATCTC160950.084.7424851
ATCTCGA167400.081.279443
TCGACGG343350.079.2831342
TCTCGAG127550.078.355714
CGACGGC352550.077.200713
TCGAGGG133400.074.784326
CGGCGCG365400.074.522036
CTCGAGG134800.074.060235
ACGGCGC367900.074.0459065
GACGGCG368650.073.944644
CGAGGGC135550.073.736297
AGGGCGC135750.073.506969
CTCGACG49450.072.35655
GAGGGCG148750.067.2170268
TCTCGAC55250.064.770094
CGCGCCG489650.055.8894429
GCGCGCC498250.055.0444688
GGCGCGC499450.054.9281657
Produced by FastQC (version 0.11.5)