##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048253_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253279 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.290546523854935 31.0 31.0 34.0 28.0 34.0 2 31.334684078745628 31.0 31.0 34.0 28.0 34.0 3 31.3697876505233 31.0 31.0 34.0 28.0 34.0 4 35.18052924753067 37.0 35.0 37.0 32.0 37.0 5 35.1254587063313 37.0 35.0 37.0 32.0 37.0 6 35.17928774006901 37.0 35.0 37.0 32.0 37.0 7 34.951677717071135 37.0 35.0 37.0 32.0 37.0 8 35.10292434635425 37.0 35.0 37.0 32.0 37.0 9 36.70686402660093 39.0 37.0 39.0 32.0 39.0 10-11 36.64708223687521 39.0 37.0 39.0 32.0 39.0 12-13 36.47557952754885 39.0 37.0 39.0 32.0 39.0 14-15 37.79577422845584 40.0 37.5 41.0 32.0 41.0 16-17 37.84110103857856 40.0 37.5 41.0 32.0 41.0 18-19 37.75082896990451 40.0 37.5 41.0 32.0 41.0 20-21 37.7906573702735 40.0 37.5 41.0 32.0 41.0 22-23 37.91051716058351 40.0 38.0 41.0 32.0 41.0 24-25 37.75785206938821 40.0 37.0 41.0 32.0 41.0 26-27 37.82797518198321 40.0 37.5 41.0 32.0 41.0 28-29 37.70504733100712 40.0 37.0 41.0 32.0 41.0 30-31 37.446383146160514 40.0 36.5 41.0 31.5 41.0 32-33 37.22408361790578 39.5 35.5 40.5 31.0 41.0 34-35 37.16838708396575 39.0 35.0 41.0 31.0 41.0 36-37 36.91283742153031 39.0 35.0 40.5 30.0 41.0 38-39 36.73461899094189 39.0 35.0 40.5 30.0 41.0 40-41 36.445365468500526 39.0 35.0 40.5 29.0 41.0 42-43 36.42222310764552 39.0 35.0 41.0 29.0 41.0 44-45 36.20976388747765 39.0 35.0 40.0 28.0 41.0 46-47 36.09032914630748 38.5 34.5 40.0 28.0 41.0 48-49 35.94441300272608 38.0 34.0 40.0 28.0 41.0 50-51 35.88092468247809 38.0 34.0 40.0 28.0 41.0 52-53 35.6890562185737 38.0 34.0 40.0 28.0 41.0 54-55 35.60715036606203 38.0 34.0 40.0 28.0 41.0 56-57 35.50519082472907 38.0 34.0 40.0 28.0 41.0 58-59 35.36648383441475 38.0 34.0 40.0 28.0 41.0 60-61 35.10702497449774 37.0 34.0 40.0 27.5 41.0 62-63 34.77766083449981 37.0 33.0 40.0 27.0 41.0 64-65 34.41571384473147 36.0 33.0 39.5 26.0 41.0 66-67 34.06494269695126 36.0 33.0 39.0 26.0 41.0 68-69 33.70422935612983 35.0 32.5 39.0 26.0 40.5 70-71 33.31791476654904 35.0 32.0 38.0 26.0 40.0 72-73 33.04169534909659 35.0 32.0 37.0 25.5 39.5 74-75 33.0229481728195 35.0 32.5 37.0 26.0 39.0 76-77 32.761345248128464 35.0 32.5 36.5 26.0 39.0 78-79 32.476703984501356 35.0 32.0 36.0 26.0 38.0 80-81 32.21398839062995 35.0 32.0 35.5 25.5 37.0 82-83 31.954673603483336 35.0 32.0 35.0 25.0 37.0 84-85 31.752907478121273 35.0 32.0 35.0 25.0 36.0 86-87 31.569341741852202 35.0 32.0 35.0 25.0 36.0 88-89 31.402737024178997 35.0 32.0 35.0 25.0 36.0 90-91 31.23566906774164 35.0 32.0 35.0 24.0 36.0 92-93 31.08465867644137 35.0 32.0 35.0 24.0 35.0 94-95 30.942031941691475 35.0 32.0 35.0 23.5 35.0 96-97 30.77524316933073 35.0 32.0 35.0 23.0 35.0 98-99 30.58928265409341 35.0 32.0 35.0 20.0 35.0 100-101 29.984521345449416 34.5 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4432062821268339 1101 2 0.4347386698457889 1101 3 0.46294771665486323 1101 4 0.4243620372889936 1101 5 0.3844580364853982 1101 6 0.4558101806986201 1101 7 0.5278063985735315 1101 8 0.5582734946885992 1101 9 0.5864802206516799 1101 10-11 0.6166398996617488 1101 12-13 0.6046015201384591 1101 14-15 0.707911192131597 1101 16-17 0.6826377153068961 1101 18-19 0.7432072350847818 1101 20-21 0.7672803243561006 1101 22-23 0.7033651858155565 1101 24-25 0.6769152415997297 1101 26-27 0.6954301400099538 1101 28-29 0.7580251171306216 1101 30-31 0.756110264201105 1101 32-33 0.7863457410321928 1101 34-35 0.8529848358427898 1101 36-37 0.8804728356655929 1101 38-39 0.8043210952864825 1101 40-41 0.7131093702713969 1101 42-43 0.7908174925554334 1101 44-45 0.7883895867953186 1101 46-47 0.7387752036251456 1101 48-49 0.7567959365793868 1101 50-51 0.7035230723281458 1101 52-53 0.6652401784912456 1101 54-55 0.5923918302865943 1101 56-57 0.5654566772771261 1101 58-59 0.5721731740170313 1101 60-61 0.564708548611236 1101 62-63 0.48035369945229434 1101 64-65 0.4505646782290498 1101 66-67 0.49007073858263084 1101 68-69 0.46963539213728467 1101 70-71 0.482426248994102 1101 72-73 0.4963527957004601 1101 74-75 0.47678907851653207 1101 76-77 0.4557598773547369 1101 78-79 0.4790028448590036 1101 80-81 0.39611715250255486 1101 82-83 0.3918751319725047 1101 84-85 0.42320889112116333 1101 86-87 0.44001920645294135 1101 88-89 0.42397812821521796 1101 90-91 0.42991080562526207 1101 92-93 0.46615239940750897 1101 94-95 0.4000933779035698 1101 96-97 0.3844006851995374 1101 98-99 0.38473528465536333 1101 100-101 0.46041291262240946 1102 1 0.34937566131139164 1102 2 0.3688978008576065 1102 3 0.3748724217988446 1102 4 0.33991786542794955 1102 5 0.3483994566747839 1102 6 0.41393250774986967 1102 7 0.43939885729845685 1102 8 0.4906200722522769 1102 9 0.56664535996034 1102 10-11 0.5914968807851508 1102 12-13 0.5724398335850012 1102 14-15 0.7113927621946559 1102 16-17 0.6542247777473307 1102 18-19 0.7032204418951125 1102 20-21 0.7952373690646439 1102 22-23 0.6741014037266311 1102 24-25 0.6238577718200062 1102 26-27 0.6487649918158453 1102 28-29 0.7137563830296116 1102 30-31 0.6899758893487515 1102 32-33 0.69776294404042 1102 34-35 0.7253110678968824 1102 36-37 0.8003789923277509 1102 38-39 0.7841743943667723 1102 40-41 0.6414532180519643 1102 42-43 0.7154548080577712 1102 44-45 0.6773670840722517 1102 46-47 0.6729433891255638 1102 48-49 0.6648599014051584 1102 50-51 0.6263452987787801 1102 52-53 0.6271408799215408 1102 54-55 0.5133424735713774 1102 56-57 0.4528087269766843 1102 58-59 0.4137945299537975 1102 60-61 0.3718187320719011 1102 62-63 0.392517375479315 1102 64-65 0.3093555290779122 1102 66-67 0.3859857988974795 1102 68-69 0.29196633998056853 1102 70-71 0.2931615653572379 1102 72-73 0.3467945151777556 1102 74-75 0.34526216405998866 1102 76-77 0.3072518308227288 1102 78-79 0.30223646323463527 1102 80-81 0.2845480906802038 1102 82-83 0.35059217291881595 1102 84-85 0.32407525701179196 1102 86-87 0.35296533939745345 1102 88-89 0.3442344712334027 1102 90-91 0.3300227015935633 1102 92-93 0.3663308712230844 1102 94-95 0.3488174231838137 1102 96-97 0.3449493074565879 1102 98-99 0.3505838037978677 1102 100-101 0.40440150370670835 1103 1 0.26196549225951316 1103 2 0.29023655287982564 1103 3 0.28127624966636944 1103 4 0.3032522895474372 1103 5 0.33810460412246357 1103 6 0.38242758455184145 1103 7 0.3859632083666611 1103 8 0.4510017717855703 1103 9 0.5030432359450216 1103 10-11 0.5115287825347892 1103 12-13 0.45214126351361017 1103 14-15 0.6036837876499277 1103 16-17 0.5621501816717114 1103 18-19 0.53398300842575 1103 20-21 0.6343296181123037 1103 22-23 0.5802425140727436 1103 24-25 0.5783031263230072 1103 26-27 0.6154529049136528 1103 28-29 0.6691884445662097 1103 30-31 0.6236906454167936 1103 32-33 0.701813254893068 1103 34-35 0.6475356424314356 1103 36-37 0.678311546644295 1103 38-39 0.6892000306949555 1103 40-41 0.5434564150755534 1103 42-43 0.5485508893389621 1103 44-45 0.6056552168396934 1103 46-47 0.5681098909615301 1103 48-49 0.5344376387522658 1103 50-51 0.5109286423672259 1103 52-53 0.5034494027566723 1103 54-55 0.41487909948088486 1103 56-57 0.3516079833016619 1103 58-59 0.4175995744366858 1103 60-61 0.34556283449650493 1103 62-63 0.3473315669424579 1103 64-65 0.3083778665092254 1103 66-67 0.3730284108259525 1103 68-69 0.3848434322086476 1103 70-71 0.3816284985923559 1103 72-73 0.3366949297549624 1103 74-75 0.2829071061045738 1103 76-77 0.3609192800160983 1103 78-79 0.38570526164986063 1103 80-81 0.3275828489483672 1103 82-83 0.2915973027526846 1103 84-85 0.285094242469377 1103 86-87 0.2509252924929406 1103 88-89 0.227918059638764 1103 90-91 0.1767445068225335 1103 92-93 0.19657409370643109 1103 94-95 0.2442521128801225 1103 96-97 0.22803180063846185 1103 98-99 0.2109477739541319 1103 100-101 0.23698361283054936 1104 1 0.3318464753491952 1104 2 0.3169390751862373 1104 3 0.31365604009210557 1104 4 0.3076856076071621 1104 5 0.3030726211204353 1104 6 0.31481300297465253 1104 7 0.3965622913410698 1104 8 0.3799385505211319 1104 9 0.43805830158002124 1104 10-11 0.4195185316533596 1104 12-13 0.4285218155754933 1104 14-15 0.5615079739443942 1104 16-17 0.5463309207299432 1104 18-19 0.5000313672304628 1104 20-21 0.5926905167411505 1104 22-23 0.5480446593951314 1104 24-25 0.4813701886432966 1104 26-27 0.519064769031857 1104 28-29 0.5948535338500704 1104 30-31 0.5338727517987891 1104 32-33 0.5950336669917817 1104 34-35 0.614717001737489 1104 36-37 0.6464389267832971 1104 38-39 0.6452001451740017 1104 40-41 0.520742261130188 1104 42-43 0.561506221524084 1104 44-45 0.4951419305737659 1104 46-47 0.5219490673364859 1104 48-49 0.513340405444751 1104 50-51 0.44406530452940274 1104 52-53 0.4688113086259449 1104 54-55 0.42888377151113133 1104 56-57 0.2994038047412033 1104 58-59 0.35209906297314575 1104 60-61 0.311734508599514 1104 62-63 0.29385693375207467 1104 64-65 0.267288076843208 1104 66-67 0.34599033223165776 1104 68-69 0.3434808828536333 1104 70-71 0.3351768422736541 1104 72-73 0.29860284329040354 1104 74-75 0.32201266592737454 1104 76-77 0.39870156380724353 1104 78-79 0.355879910205644 1104 80-81 0.3099817324271754 1104 82-83 0.29305102399144545 1104 84-85 0.32809086763196404 1104 86-87 0.3137790099185054 1104 88-89 0.3167408908333549 1104 90-91 0.2854824624811769 1104 92-93 0.2870395824654395 1104 94-95 0.313177567201123 1104 96-97 0.362231006953202 1104 98-99 0.32803487369621465 1104 100-101 0.3718635606441225 1105 1 0.1773324988634215 1105 2 0.17493981903600186 1105 3 0.17200679269209118 1105 4 0.17212902064053992 1105 5 0.15441982200825066 1105 6 0.18357610192225593 1105 7 0.1310699072782171 1105 8 0.1507194921225974 1105 9 0.2605172883793685 1105 10-11 0.2625860896734835 1105 12-13 0.24253631366521233 1105 14-15 0.23202645768570562 1105 16-17 0.2954209142135511 1105 18-19 0.2954083661034872 1105 20-21 0.3059797410172038 1105 22-23 0.31949974528527036 1105 24-25 0.21398975007389254 1105 26-27 0.3327051100403793 1105 28-29 0.41952220251483396 1105 30-31 0.3859411273718152 1105 32-33 0.27116101660409697 1105 34-35 0.3491828755596629 1105 36-37 0.48957178676064217 1105 38-39 0.36309211438882016 1105 40-41 0.2788910119268806 1105 42-43 0.3206159135520963 1105 44-45 0.17189660106390647 1105 46-47 0.22953521884092964 1105 48-49 0.3277985672234749 1105 50-51 0.20799529969486485 1105 52-53 0.16486793082071216 1105 54-55 0.16522690817396324 1105 56-57 0.04970586180562009 1105 58-59 0.029746897026143415 1105 60-61 -0.11152633266178213 1105 62-63 -0.03381150958238521 1105 64-65 -0.09206373162732717 1105 66-67 0.014300104099334021 1105 68-69 0.023824936909534244 1105 70-71 0.10063282120559336 1105 72-73 0.07406429963201333 1105 74-75 0.011699400790547543 1105 76-77 0.03557887372701174 1105 78-79 0.030155216763631643 1105 80-81 0.03483726858830494 1105 82-83 0.04929348524778021 1105 84-85 0.006821533138861469 1105 86-87 -0.0089562256701754 1105 88-89 -0.04338872929026394 1105 90-91 -0.0828196590329 1105 92-93 0.04658079502172541 1105 94-95 -0.009917734207384399 1105 96-97 -0.009971934483971268 1105 98-99 -0.019685507625005982 1105 100-101 0.03570650817885124 1106 1 0.09542069472917092 1106 2 0.17211096581712582 1106 3 0.19263914406988647 1106 4 0.08171439752874932 1106 5 0.1386626439209948 1106 6 0.15743373688852813 1106 7 0.19276387001065132 1106 8 0.18476214899612842 1106 9 0.2853889298104306 1106 10-11 0.29296999641570665 1106 12-13 0.27420936017970376 1106 14-15 0.32167965790560515 1106 16-17 0.3273541888679574 1106 18-19 0.2688385104988953 1106 20-21 0.3669012595979595 1106 22-23 0.2555669815116772 1106 24-25 0.18840156874513525 1106 26-27 0.20169059920481658 1106 28-29 0.32695803081489316 1106 30-31 0.291597192076388 1106 32-33 0.3125161173916666 1106 34-35 0.4162314245329881 1106 36-37 0.43107015760512724 1106 38-39 0.43818177450991413 1106 40-41 0.37151401108233273 1106 42-43 0.34843764996034565 1106 44-45 0.28125563998855796 1106 46-47 0.2605395395539958 1106 48-49 0.31058522394935295 1106 50-51 0.2808209323357218 1106 52-53 0.2310355927663963 1106 54-55 0.21829498514473045 1106 56-57 0.15901118492715227 1106 58-59 0.20578073385802753 1106 60-61 0.1311964124500946 1106 62-63 0.17911352549919002 1106 64-65 0.1448130844708686 1106 66-67 0.17303068155835888 1106 68-69 0.13806115586307754 1106 70-71 0.14263933770123316 1106 72-73 0.12544613965496154 1106 74-75 0.04589119624557014 1106 76-77 0.05224606815006183 1106 78-79 0.06060674426757373 1106 80-81 0.11773630108129396 1106 82-83 0.1817925084244365 1106 84-85 0.14552359784824276 1106 86-87 0.06895911955139056 1106 88-89 0.1359118563486703 1106 90-91 0.10199675506283512 1106 92-93 0.12909484187410314 1106 94-95 0.09088022712343147 1106 96-97 0.03264164406204628 1106 98-99 0.03047767338456353 1106 100-101 0.1087097766657763 1107 1 0.04959991825015564 1107 2 0.06559039494134211 1107 3 0.12748148920044144 1107 4 0.0929900983264389 1107 5 0.14008559657492725 1107 6 0.13337242573400943 1107 7 0.14526996455411734 1107 8 -0.10369107335493055 1107 9 -2.2297054366759994E-4 1107 10-11 -0.0034356719585986184 1107 12-13 -0.022366914461102283 1107 14-15 -0.10919739092098979 1107 16-17 -0.0645684974499261 1107 18-19 0.030286195706395347 1107 20-21 0.04279743862870333 1107 22-23 0.048299362461364126 1107 24-25 -0.038590667346717566 1107 26-27 0.04427481006106149 1107 28-29 -0.011405179090353101 1107 30-31 0.028535921557413246 1107 32-33 0.0505022987880821 1107 34-35 0.12458510713666726 1107 36-37 0.29371945777607067 1107 38-39 0.16825999634909294 1107 40-41 -0.0817223025370879 1107 42-43 0.0034946247672564823 1107 44-45 0.02203561712555313 1107 46-47 0.06230957169386642 1107 48-49 0.1005464107087235 1107 50-51 0.029297745350184812 1107 52-53 0.14243683848194166 1107 54-55 0.13188169051911558 1107 56-57 -0.10344159988309087 1107 58-59 0.07868238073140787 1107 60-61 -0.12792685687754357 1107 62-63 -0.025121721516065065 1107 64-65 -0.1994237170900348 1107 66-67 0.04471449075276723 1107 68-69 0.10080060235662103 1107 70-71 0.24382408419059232 1107 72-73 0.2238981224645542 1107 74-75 0.20238604702966256 1107 76-77 0.20239002506252746 1107 78-79 0.2422430004302356 1107 80-81 0.21489194716905047 1107 82-83 0.24065333201547645 1107 84-85 0.20003647380180212 1107 86-87 0.252505183085848 1107 88-89 0.21134847577415883 1107 90-91 0.2333524999989045 1107 92-93 0.24128010938038358 1107 94-95 0.23467084888184075 1107 96-97 0.16470714067716585 1107 98-99 0.08987183056094139 1107 100-101 0.10347069006451193 1108 1 -0.2167681558720389 1108 2 -0.11795388133825568 1108 3 -4.6222350043123583E-4 1108 4 -0.1372422077653681 1108 5 -0.008612954969784425 1108 6 0.07920947939685874 1108 7 0.10737320204420087 1108 8 0.15918847114441093 1108 9 0.22383542639496312 1108 10-11 0.20432971382250997 1108 12-13 0.2454152734518189 1108 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-0.6704783654677939 2215 12-13 -0.5749047618965619 2215 14-15 -0.7910206093547529 2215 16-17 -0.7634297368700018 2215 18-19 -0.6946390439272818 2215 20-21 -3.6096689859567803 2215 22-23 -1.381032733765906 2215 24-25 -0.980441787417206 2215 26-27 -0.8170193452803005 2215 28-29 -0.7616935405033232 2215 30-31 -1.218643209620332 2215 32-33 -1.080463845446019 2215 34-35 -1.140758790548432 2215 36-37 -1.3145255426244304 2215 38-39 -1.0580115973817144 2215 40-41 -1.2898004271198928 2215 42-43 -1.6137049320105703 2215 44-45 -1.082980669954253 2215 46-47 -0.9762754548727557 2215 48-49 -0.999643827723439 2215 50-51 -0.9050940256694986 2215 52-53 -0.7418394544718936 2215 54-55 -0.7112864135045953 2215 56-57 -0.6424671786084275 2215 58-59 -0.4964604649685356 2215 60-61 -0.5235794080750438 2215 62-63 -0.634462094783899 2215 64-65 -0.5657615271453054 2215 66-67 -0.6545058654269837 2215 68-69 -0.5328649251044553 2215 70-71 -0.4806859684086646 2215 72-73 -0.4470654852571343 2215 74-75 -0.462366442757542 2215 76-77 -0.5459165191395456 2215 78-79 -0.5215008919148332 2215 80-81 -0.5178250581442754 2215 82-83 -0.5063315557104744 2215 84-85 -0.6277904025498948 2215 86-87 -0.6338630651185717 2215 88-89 -0.6168114847096469 2215 90-91 -0.6035257207043756 2215 92-93 -0.5206928967873452 2215 94-95 -0.5662876858149808 2215 96-97 -0.5571088687212793 2215 98-99 -0.5185974509385396 2215 100-101 -0.5741479252667041 2216 1 -0.457191013592702 2216 2 -0.39987288346076255 2216 3 -0.36647801660997814 2216 4 -0.38973461156052736 2216 5 -0.36666864050837944 2216 6 -0.42053666480411067 2216 7 -0.6250174505245099 2216 8 -1.5635889903544609 2216 9 -0.9072736692665089 2216 10-11 -0.7637960166947195 2216 12-13 -0.732746862171858 2216 14-15 -1.4980362905678888 2216 16-17 -0.9409415375056085 2216 18-19 -0.8837086566686025 2216 20-21 -0.873118560192296 2216 22-23 -0.7899593974315451 2216 24-25 -0.9948106802102927 2216 26-27 -1.2080824829517738 2216 28-29 -1.8220238389249062 2216 30-31 -1.0163412200558 2216 32-33 -0.8981449390131075 2216 34-35 -2.2231172719958465 2216 36-37 -2.1905664822925033 2216 38-39 -2.2946345390195972 2216 40-41 -1.4288253548567837 2216 42-43 -1.3800690657524655 2216 44-45 -1.1785065722740171 2216 46-47 -1.4262572562434883 2216 48-49 -1.4264845767444285 2216 50-51 -0.9841485925661786 2216 52-53 -0.8077296533954552 2216 54-55 -0.8093424155836715 2216 56-57 -0.8032018361986033 2216 58-59 -0.8755969454912815 2216 60-61 -0.9281605780118127 2216 62-63 -0.8374111543356193 2216 64-65 -0.7957527265732338 2216 66-67 -0.8463276325711391 2216 68-69 -0.8838651630022056 2216 70-71 -0.967509997895803 2216 72-73 -0.8521286402258283 2216 74-75 -0.7108794067533069 2216 76-77 -0.812111357818253 2216 78-79 -0.8475013871392463 2216 80-81 -0.8719814913810424 2216 82-83 -1.14764315065527 2216 84-85 -0.8173159098351661 2216 86-87 -1.0070402178114684 2216 88-89 -0.7925982983044619 2216 90-91 -0.9465398939527674 2216 92-93 -1.2037785834009824 2216 94-95 -0.919801799710747 2216 96-97 -0.8389900289923062 2216 98-99 -0.8632712137103908 2216 100-101 -0.8973804306009754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 106.0 11 1405.0 12 5084.0 13 10526.0 14 14569.0 15 17771.0 16 19732.0 17 23517.0 18 32190.0 19 50575.0 20 50123.0 21 50593.0 22 58119.0 23 65915.0 24 73046.0 25 81889.0 26 95440.0 27 112111.0 28 133148.0 29 154885.0 30 177469.0 31 209296.0 32 254619.0 33 320477.0 34 418055.0 35 594432.0 36 981075.0 37 1582750.0 38 1414270.0 39 249892.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.774021437266644 39.297966636152914 12.593655918043947 20.334356008536492 2 23.842214277822784 27.0013797030142 32.235961628725406 16.92044439043761 3 22.275757654340676 27.85136639036682 22.748693309379867 27.124182645912637 4 25.95327801054256 25.623374624021878 25.249769212475165 23.1735781529604 5 23.134323108737597 29.16975911137311 23.742085904700243 23.953831875189046 6 22.561712997091302 24.577656653205622 26.114176456328554 26.74645389337452 7 26.695123387381248 25.47647356197445 23.311978381938395 24.51642466870591 8 27.690259146459166 24.29480129678022 24.952636525387923 23.062303031372682 9 21.97686278846029 25.92700643781854 25.171754667466693 26.924376106254478 10-11 26.232055286376088 24.82346622680658 24.17472636791227 24.769752118905057 12-13 25.38492638632059 25.040962742499467 23.97893354519275 25.59517732598719 14-15 25.221213316090324 24.835947818749844 24.345880014007648 25.596958851152184 16-17 25.349018105885108 26.852248990352912 24.15989352123185 23.63883938253013 18-19 23.221834160836092 24.82750248581719 26.296319887585362 25.654343465761347 20-21 23.137739930335073 27.62354935683652 24.629199748154537 24.60951096467387 22-23 22.239335191590833 26.943612256501865 27.49032715816288 23.32672539374442 24-25 22.122936096978076 25.25746451806714 29.206105304947744 23.413494080007037 26-27 22.071935089636717 25.033448697760345 30.543095717911743 22.351520494691194 28-29 22.097823383050276 24.883762907495157 30.831373410987446 22.187040298467124 30-31 22.19141533617661 24.801618199073147 30.732057504992415 22.274908959757823 32-33 22.10856740887523 24.7858154675026 30.821732619058583 22.28388450456358 34-35 22.160134405826238 24.995667463596007 30.748586462605697 22.095611667972058 36-37 22.156593420797297 24.94489067234517 30.540263398355112 22.358252508502417 38-39 22.329162231738707 24.977068443620702 30.306358699110476 22.387410625530116 40-41 22.9531893643827 25.655929888553064 28.40006628854987 22.990814458514368 42-43 22.98262164657822 25.82983283429608 28.23058864566448 22.956956873461227 44-45 23.14727693875507 26.138040423875843 27.777742926848877 22.936939710520218 46-47 23.080292375351892 26.619539934972856 27.50220279683161 22.797964892843638 48-49 23.094024095860643 26.739044506629345 27.21074151428616 22.95618988322385 50-51 23.01771576667732 26.69877750628102 27.288457435386242 22.99504929165542 52-53 23.035232556768825 26.830483083838125 27.197058462830643 22.937225896562406 54-55 23.06975477042101 26.630145175546776 27.2385211195077 23.061578934524515 56-57 23.030687224357425 26.501352009208524 27.515534698168924 22.952426068265126 58-59 23.09722947600168 26.635106062051662 27.42429811755921 22.84336634438745 60-61 23.039352270883278 26.5839836024507 27.38549006594121 22.991174060724813 62-63 23.092803958044357 26.533220354545854 27.458912031372297 22.915063656037496 64-65 23.207264845023268 26.722248256991715 27.188275226623134 22.882211671361887 66-67 23.240629915679946 26.667580279285584 26.974407977988925 23.11738182704555 68-69 23.238503578864126 26.720280579307648 26.933935672404164 23.10728016942406 70-71 23.350743351261745 26.908690433787186 26.71361790062441 23.026948314326663 72-73 23.326332842161502 26.87790846969815 26.558857034528515 23.236901653611834 74-75 23.304464684753707 26.859390270813428 26.711952866908494 23.124192177524368 76-77 23.390563797974114 26.998254546657783 26.537763696500683 23.073417958867413 78-79 23.438067955157045 26.900582820007283 26.479772532893275 23.1815766919424 80-81 23.429706688689084 26.8290888936837 26.586382728210868 23.154821689416348 82-83 23.534257495414785 27.012563142811015 26.385336625856638 23.067842735917562 84-85 23.594384002049278 26.87401105072616 26.38718295546056 23.144421991764 86-87 23.59685185141782 26.833222601805335 26.473054462678192 23.096871084098655 88-89 23.706514233529017 26.968597717570063 26.275090636599714 23.04979741230121 90-91 23.7576687729784 26.92419525017582 26.18430229969094 23.13383367715484 92-93 23.698323199755585 26.891410078117772 26.309762798315077 23.100503923811562 94-95 23.83956276878361 27.060209596239165 26.089910508061248 23.01031712691598 96-97 23.862731600425132 27.025122017228348 26.029806657099495 23.08233972524702 98-99 23.905912070940605 26.962874308295596 26.14900791766041 22.982205703103382 100-101 24.093930483027055 27.124663204048815 25.8150692948665 22.966337018057626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5534.0 1 4198.5 2 2386.0 3 1646.0 4 1200.0 5 874.5 6 647.0 7 522.5 8 439.5 9 399.0 10 378.0 11 332.5 12 327.0 13 415.0 14 489.0 15 685.0 16 7183.5 17 7926.0 18 2421.0 19 2769.0 20 3611.5 21 4888.0 22 6724.5 23 9235.0 24 12155.5 25 15965.5 26 21013.0 27 27589.0 28 35139.0 29 44781.5 30 57267.5 31 70792.5 32 85857.5 33 105679.5 34 127226.5 35 148672.5 36 169301.0 37 186093.0 38 201940.5 39 219600.0 40 229816.0 41 230435.0 42 232392.5 43 240642.0 44 252390.5 45 265700.0 46 276956.0 47 285190.5 48 289969.5 49 289056.0 50 284091.5 51 279371.5 52 278962.5 53 277031.5 54 264386.5 55 247008.0 56 228930.0 57 204137.5 58 181040.5 59 163090.0 60 143007.0 61 120860.0 62 98190.0 63 76746.5 64 58764.0 65 43126.5 66 30850.5 67 22323.0 68 16490.5 69 12564.5 70 9861.5 71 7476.0 72 6008.0 73 4658.5 74 3327.5 75 2473.0 76 1743.0 77 1126.0 78 709.0 79 418.5 80 181.5 81 68.5 82 37.5 83 20.5 84 11.5 85 8.5 86 9.5 87 5.5 88 2.0 89 1.5 90 0.5 91 1.5 92 3.5 93 3.5 94 5.5 95 6.0 96 3.0 97 3.0 98 3.5 99 3.5 100 20.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08648502284277222 2 0.04367679776277736 3 0.05055644488513402 4 0.057725616235084855 5 0.04249112711643934 6 0.017109503164017267 7 0.02157644838975586 8 0.011787772123476844 9 0.0027711604641156087 10-11 8.754661167728417E-4 12-13 5.721550211979988E-4 14-15 0.0013648999300867925 16-17 2.7573735961349343E-5 18-19 0.001806079705468382 20-21 0.00576980424991235 22-23 0.015779070403882163 24-25 0.02005299947789131 26-27 0.01040219189141904 28-29 0.017798846563051 30-31 4.204994734105775E-4 32-33 0.0058042714198640365 34-35 3.0331109557484276E-4 36-37 0.002709119558202573 38-39 0.009905864644114751 40-41 0.0019439483852751287 42-43 0.002019776159168839 44-45 0.0022679397828209834 46-47 0.0 48-49 0.0 50-51 0.0038120689966565464 52-53 0.0027642670301252716 54-55 0.002729799860173585 56-57 0.0 58-59 0.004894338133139509 60-61 0.0 62-63 0.0 64-65 0.002178325140946598 66-67 3.102045295651801E-4 68-69 0.0 70-71 0.0010960560044636363 72-73 0.0029848569178160663 74-75 0.005108034586839966 76-77 0.002054243329120526 78-79 1.4476211379708403E-4 80-81 8.82359550763179E-4 82-83 0.0010753757024926243 84-85 0.0 86-87 0.0 88-89 6.893433990337336E-6 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7253279.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.87738820614272 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57365619144036 45.02245466197389 2 10.725093741164894 11.985804530446849 3 3.346667096052565 5.610090495685604 4 1.495978941670106 3.3436558428766014 5 0.8314878086239255 2.3230683535576997 6 0.5344899717309942 1.7919542185621806 7 0.3923871714141078 1.5347899212951457 8 0.2819021746402456 1.2601565798823076 9 0.22976056857954819 1.1554578436485143 >10 1.3809724433177055 14.30714497557661 >50 0.14492452969688197 5.657866443238281 >100 0.062108524702443886 5.075829771107915 >500 2.233879781462929E-4 0.08082858981648801 >1k 2.729956335963618E-4 0.2790424385008084 >5k 4.9635569686800075E-5 0.17109758490695848 >10k+ 2.4817784843400038E-5 0.4007577489241525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 28899 0.39842669777351736 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 2.7573735961349343E-5 0.0 0.0 0.0 0.0 4 4.136060394202401E-5 0.0 0.0 0.0 1.3786867980674671E-5 5 4.136060394202401E-5 1.3786867980674671E-5 0.0 0.0 1.3786867980674671E-5 6 8.272120788404803E-5 1.3786867980674671E-5 0.0 1.3786867980674671E-5 1.3786867980674671E-5 7 9.65080758647227E-5 1.3786867980674671E-5 0.0 1.3786867980674671E-5 1.3786867980674671E-5 8 1.5165554778742138E-4 1.3786867980674671E-5 0.0 1.3786867980674671E-5 1.3786867980674671E-5 9 2.757373596134934E-4 1.3786867980674671E-5 0.0 1.3786867980674671E-5 1.3786867980674671E-5 10-11 5.376878512463122E-4 1.3786867980674671E-5 0.0 1.3786867980674671E-5 2.0680301971012006E-5 12-13 8.203186448501429E-4 1.3786867980674671E-5 0.0 1.3786867980674671E-5 4.136060394202401E-5 14-15 0.0011374166084056605 1.3786867980674671E-5 0.0 2.7573735961349343E-5 6.204090591303602E-5 16-17 0.0014338342699901658 2.7573735961349343E-5 0.0 2.7573735961349343E-5 1.0340150985506004E-4 18-19 0.001619956987729274 2.7573735961349343E-5 0.0 2.7573735961349343E-5 1.1029494384539737E-4 20-21 0.0018819074793620927 2.7573735961349343E-5 0.0 2.7573735961349343E-5 1.1718837783573471E-4 22-23 0.0023644478586857063 2.7573735961349343E-5 0.0 3.4467169951686676E-5 1.378686798067467E-4 24-25 0.002971070049835392 2.7573735961349343E-5 0.0 7.582777389371069E-5 1.585489817777587E-4 26-27 0.003763814958724185 2.7573735961349343E-5 0.0 8.272120788404803E-5 1.7922928374877072E-4 28-29 0.004728895717371413 2.7573735961349343E-5 0.0 8.272120788404803E-5 1.8612271773910805E-4 30-31 0.006279918365197312 3.4467169951686676E-5 0.0 8.272120788404803E-5 1.930161517294454E-4 32-33 0.008030850598742995 4.136060394202401E-5 0.0 9.65080758647227E-5 1.930161517294454E-4 34-35 0.010112667663824872 4.136060394202401E-5 0.0 1.1029494384539737E-4 1.930161517294454E-4 36-37 0.013573171526974213 4.136060394202401E-5 0.0 1.1029494384539737E-4 2.0680301971012007E-4 38-39 0.018157305130548543 4.825403793236135E-5 0.0 1.1718837783573471E-4 2.4127018966180674E-4 40-41 0.024051191192286964 5.5147471922698686E-5 0.0 1.3097524581640935E-4 2.619504916328187E-4 42-43 0.03275759832208302 5.5147471922698686E-5 0.0 1.4476211379708405E-4 2.8263079360383073E-4 44-45 0.0429460937598016 5.5147471922698686E-5 0.0 1.5165554778742138E-4 3.0331109557484276E-4 46-47 0.05623663449317198 5.5147471922698686E-5 0.0 1.5165554778742138E-4 3.3088483153619216E-4 48-49 0.07290495788180766 5.5147471922698686E-5 0.0 1.6544241576809605E-4 3.5845856749754145E-4 50-51 0.0940333330621916 6.204090591303602E-5 0.0 1.7922928374877072E-4 3.5845856749754145E-4 52-53 0.11920126056091321 6.893433990337335E-5 0.0 1.7922928374877072E-4 3.7913886946855347E-4 54-55 0.14841563381196285 8.272120788404803E-5 0.0 1.7922928374877072E-4 3.929257374492281E-4 56-57 0.1823657962143742 8.272120788404803E-5 0.0 1.8612271773910805E-4 4.204994734105775E-4 58-59 0.222781999699722 8.272120788404803E-5 0.0 1.930161517294454E-4 4.2739290740091484E-4 60-61 0.269312679134499 8.272120788404803E-5 0.0 1.930161517294454E-4 4.6186007735260146E-4 62-63 0.3232744804108597 8.272120788404803E-5 0.0 1.9990958571978275E-4 4.7564694533327616E-4 64-65 0.3839987404317413 8.272120788404803E-5 0.0 2.0680301971012007E-4 5.307944172559748E-4 66-67 0.4534569813183803 8.272120788404803E-5 0.0 2.0680301971012007E-4 5.583681532173242E-4 68-69 0.5335380039841291 8.272120788404803E-5 0.0 2.136964537004574E-4 5.859418891786736E-4 70-71 0.6210225747555003 9.65080758647227E-5 0.0 2.2058988769079474E-4 6.066221911496855E-4 72-73 0.7205086141040487 9.65080758647227E-5 0.0 2.274833216811321E-4 6.066221911496855E-4 74-75 0.8322442856534266 9.65080758647227E-5 0.0 2.3437675567146942E-4 6.066221911496855E-4 76-77 0.9590421104716915 9.65080758647227E-5 0.0 2.4127018966180674E-4 6.204090591303603E-4 78-79 1.0973795437897813 9.65080758647227E-5 0.0 2.4816362365214406E-4 6.341959271110349E-4 80-81 1.2481389451584586 9.65080758647227E-5 0.0 2.4816362365214406E-4 6.548762290820469E-4 82-83 1.4158699810113466 9.65080758647227E-5 0.0 2.4816362365214406E-4 6.617696630723842E-4 84-85 1.597360311108948 1.1029494384539736E-4 0.0 2.4816362365214406E-4 6.755565310530588E-4 86-87 1.7982418710213683 1.2408181182607203E-4 0.0 2.4816362365214406E-4 6.893433990337336E-4 88-89 2.014640550846038 1.2408181182607203E-4 0.0 2.5505705764248144E-4 7.100237010047455E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 30260 0.0 89.73894 1 TATCTCG 15850 0.0 85.84339 2 ATATCTC 16095 0.0 84.742485 1 ATCTCGA 16740 0.0 81.27944 3 TCGACGG 34335 0.0 79.283134 2 TCTCGAG 12755 0.0 78.35571 4 CGACGGC 35255 0.0 77.20071 3 TCGAGGG 13340 0.0 74.78432 6 CGGCGCG 36540 0.0 74.52203 6 CTCGAGG 13480 0.0 74.06023 5 ACGGCGC 36790 0.0 74.045906 5 GACGGCG 36865 0.0 73.94464 4 CGAGGGC 13555 0.0 73.73629 7 AGGGCGC 13575 0.0 73.50696 9 CTCGACG 4945 0.0 72.3565 5 GAGGGCG 14875 0.0 67.217026 8 TCTCGAC 5525 0.0 64.77009 4 CGCGCCG 48965 0.0 55.889442 9 GCGCGCC 49825 0.0 55.044468 8 GGCGCGC 49945 0.0 54.928165 7 >>END_MODULE