FastQCFastQC Report
Tue 31 May 2016
SRR2048253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253279
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT338480.4666579074098762No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT317050.43711264932729044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105960.14608565312322883No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT95020.13100281955237073No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75800.10450445929351401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG73850.072.96251
ATATCTC73050.071.745131
TCGACGG87150.063.4633142
CGACGGC97600.056.473583
TATCTCG93350.056.245152
ACGGCGC115650.047.970335
GACGGCG115850.047.7002684
CGGCGCG119350.046.4433866
ATCTCGA139200.037.548333
TCTCGAG133050.035.9626544
GGTCGAC6200.034.48721
CTCGAGG150300.031.7601495
TCGAGGG153000.031.2617666
CGAGGGC154900.030.8169827
AGGGCGC165400.028.9180769
GGGCGCG163750.028.2522548
GAGGGCG175000.027.413148
CGCGCCG274550.024.5491479
GGCGCGC286200.023.6328357
GCGCGCC286250.023.595528
Produced by FastQC (version 0.11.5)