##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048253_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253279 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26104028260873 33.0 31.0 34.0 30.0 34.0 2 32.39112351806679 34.0 31.0 34.0 30.0 34.0 3 32.47041027926818 34.0 31.0 34.0 31.0 34.0 4 36.03097633497898 37.0 35.0 37.0 35.0 37.0 5 35.88535722946822 37.0 35.0 37.0 35.0 37.0 6 35.86998294150825 37.0 35.0 37.0 35.0 37.0 7 35.84347148372481 37.0 35.0 37.0 35.0 37.0 8 35.812439449799186 37.0 35.0 37.0 33.0 37.0 9 37.397168646070284 39.0 37.0 39.0 34.0 39.0 10-11 37.39536325570821 39.0 37.0 39.0 34.0 39.0 12-13 37.39998240795646 39.0 37.0 39.0 34.0 39.0 14-15 38.734589762781766 40.0 38.0 41.0 34.0 41.0 16-17 38.74691949668557 40.0 38.0 41.0 34.0 41.0 18-19 38.78444997083388 40.0 38.0 41.0 34.0 41.0 20-21 38.80490292735189 40.0 38.0 41.0 34.5 41.0 22-23 38.7856319879602 40.0 38.0 41.0 34.0 41.0 24-25 38.76983368487549 40.0 38.0 41.0 34.0 41.0 26-27 38.678331758643225 40.0 38.0 41.0 34.0 41.0 28-29 38.58112572258698 40.0 38.0 41.0 34.0 41.0 30-31 38.43996439403475 40.0 38.0 41.0 34.0 41.0 32-33 38.32855650527162 40.0 38.0 41.0 34.0 41.0 34-35 38.27917794145241 40.0 37.5 41.0 34.0 41.0 36-37 38.194316322314364 40.0 37.0 41.0 34.0 41.0 38-39 38.09594632992885 40.0 37.0 41.0 33.0 41.0 40-41 37.94185436683188 40.0 37.0 41.0 33.0 41.0 42-43 37.85931521453952 40.0 37.0 41.0 33.0 41.0 44-45 37.73900597233334 40.0 37.0 41.0 33.0 41.0 46-47 37.622197629513494 40.0 36.5 41.0 32.5 41.0 48-49 37.50527561396714 40.0 36.0 41.0 32.0 41.0 50-51 37.40686239975051 39.0 36.0 41.0 32.0 41.0 52-53 37.37225005407899 39.0 36.0 41.0 32.0 41.0 54-55 37.18775377315556 39.0 35.0 41.0 32.0 41.0 56-57 36.93658964449044 39.0 35.0 41.0 31.0 41.0 58-59 36.680579845336155 39.0 35.0 40.5 31.0 41.0 60-61 36.39944844255957 38.0 35.0 40.0 31.0 41.0 62-63 36.0721424062138 37.5 35.0 40.0 31.0 41.0 64-65 35.74410263275409 37.0 35.0 40.0 30.0 41.0 66-67 35.39480399140858 36.5 34.0 39.0 30.0 41.0 68-69 35.03093959297581 36.0 34.0 39.0 30.0 41.0 70-71 34.67029918468599 35.5 34.0 39.0 29.0 40.5 72-73 34.29888020300887 35.0 34.0 37.5 29.0 40.0 74-75 33.96051082551767 35.0 34.0 37.0 29.0 39.0 76-77 33.720426375436546 35.0 34.0 36.5 29.0 39.0 78-79 33.57906079443518 35.0 34.0 36.0 29.0 38.5 80-81 33.34706964946474 35.0 34.0 36.0 29.0 37.0 82-83 33.12702944420034 35.0 34.0 35.5 29.0 37.0 84-85 32.93934956865716 35.0 33.0 35.0 29.0 36.5 86-87 32.75171739567718 35.0 33.0 35.0 29.0 36.0 88-89 32.62586204115408 35.0 33.0 35.0 29.0 36.0 90-91 32.49847289756812 35.0 33.0 35.0 29.0 36.0 92-93 32.363775611002964 35.0 33.0 35.0 29.0 35.5 94-95 32.251792258370315 35.0 33.0 35.0 29.0 35.0 96-97 32.13992774853966 35.0 33.0 35.0 29.0 35.0 98-99 31.99392764293225 35.0 33.0 35.0 27.5 35.0 100-101 31.53772500685552 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34184353900042197 1101 2 0.3863540399033809 1101 3 0.32627077465498644 1101 4 0.2524531091049198 1101 5 0.26380377940361655 1101 6 0.2629668149820077 1101 7 0.2611325850949058 1101 8 0.2858627527972857 1101 9 0.36236916660427454 1101 10-11 0.3612601246479912 1101 12-13 0.35508745579597445 1101 14-15 0.4246683596012346 1101 16-17 0.42783420771539227 1101 18-19 0.45702307659691854 1101 20-21 0.4231753797250519 1101 22-23 0.49048599851022345 1101 24-25 0.4706115713471206 1101 26-27 0.48383594376574024 1101 28-29 0.4989214558000725 1101 30-31 0.48249543210998525 1101 32-33 0.47247757357737896 1101 34-35 0.49006442626562574 1101 36-37 0.48828495408891115 1101 38-39 0.47481182086956153 1101 40-41 0.5114260250649494 1101 42-43 0.4175162090607145 1101 44-45 0.43762908037739834 1101 46-47 0.4644636452147637 1101 48-49 0.4898891923555766 1101 50-51 0.45149796077940607 1101 52-53 0.4759474125982379 1101 54-55 0.4540483907282322 1101 56-57 0.4100947797546155 1101 58-59 0.3559286388927916 1101 60-61 0.3577875354702087 1101 62-63 0.39773847371829874 1101 64-65 0.34195748456373565 1101 66-67 0.3267571599673076 1101 68-69 0.27463471693017283 1101 70-71 0.2522056689889496 1101 72-73 0.24651365356480426 1101 74-75 0.22560085705761423 1101 76-77 0.27629721604128576 1101 78-79 0.27028041006313686 1101 80-81 0.22270582555025698 1101 82-83 0.2210277449182243 1101 84-85 0.24118429679325004 1101 86-87 0.24144711709640632 1101 88-89 0.2227631915168118 1101 90-91 0.22965584121200777 1101 92-93 0.2255828750786648 1101 94-95 0.25845060349571725 1101 96-97 0.2818924711628483 1101 98-99 0.2614523872729393 1101 100-101 0.28156453647820356 1102 1 0.33163454025174843 1102 2 0.3220526160211179 1102 3 0.28595752142360453 1102 4 0.20077563614377425 1102 5 0.24283692992788275 1102 6 0.21631554109044515 1102 7 0.23222289029055077 1102 8 0.2173512040983141 1102 9 0.24824680834559842 1102 10-11 0.336199948490723 1102 12-13 0.32840195342355827 1102 14-15 0.4149177979758818 1102 16-17 0.40293160739976486 1102 18-19 0.3549149974704875 1102 20-21 0.412425049121353 1102 22-23 0.4240491543267382 1102 24-25 0.4043367283920105 1102 26-27 0.4113356002673072 1102 28-29 0.4216350240531028 1102 30-31 0.42166482039670683 1102 32-33 0.4290981341581457 1102 34-35 0.4026323728558481 1102 36-37 0.4006227025509759 1102 38-39 0.40261803834285814 1102 40-41 0.44050172605097515 1102 42-43 0.38279074557622295 1102 44-45 0.39363571282280674 1102 46-47 0.42890825484666806 1102 48-49 0.39783324697204137 1102 50-51 0.41249196505222585 1102 52-53 0.38541136866857784 1102 54-55 0.38681404938051855 1102 56-57 0.40696773041263157 1102 58-59 0.3829023895445687 1102 60-61 0.33755967356481165 1102 62-63 0.3402426834703576 1102 64-65 0.32335684299101786 1102 66-67 0.3061141179018634 1102 68-69 0.3109062267585614 1102 70-71 0.23750692686503072 1102 72-73 0.21443381716107268 1102 74-75 0.24535397595835207 1102 76-77 0.24193488999560486 1102 78-79 0.21883847083353203 1102 80-81 0.1816711129208315 1102 82-83 0.1463419842176492 1102 84-85 0.1191728306639348 1102 86-87 0.15894345780869656 1102 88-89 0.14471871833070793 1102 90-91 0.0718039958777581 1102 92-93 0.14294002632589553 1102 94-95 0.0842027133562624 1102 96-97 0.1412130811120278 1102 98-99 0.21019753657664353 1102 100-101 0.23023495265960037 1103 1 0.2737522419988565 1103 2 0.30348765542532163 1103 3 0.26277635668229493 1103 4 0.1654881062693221 1103 5 0.18361183040344997 1103 6 0.23253469365447188 1103 7 0.21660922463055954 1103 8 0.23613391671440098 1103 9 0.2775940637913763 1103 10-11 0.30113991708550003 1103 12-13 0.32508206979024834 1103 14-15 0.4041793000804361 1103 16-17 0.3871928088069012 1103 18-19 0.38943884577258814 1103 20-21 0.4126634882684357 1103 22-23 0.45291099209951824 1103 24-25 0.42675225114217596 1103 26-27 0.42965514039929076 1103 28-29 0.41800176447803494 1103 30-31 0.45744262190687124 1103 32-33 0.4660763283513276 1103 34-35 0.4213594018732536 1103 36-37 0.46369740114192837 1103 38-39 0.4422002765204738 1103 40-41 0.4118927737428706 1103 42-43 0.4347213878423801 1103 44-45 0.5038138776525116 1103 46-47 0.47443216286023215 1103 48-49 0.4840513444198393 1103 50-51 0.5012666241137822 1103 52-53 0.464835553430774 1103 54-55 0.48846182418746054 1103 56-57 0.43128154637853555 1103 58-59 0.3907898435276991 1103 60-61 0.39056974896174523 1103 62-63 0.4367499105885173 1103 64-65 0.3821712219352662 1103 66-67 0.4340198662735517 1103 68-69 0.39391277297361427 1103 70-71 0.3373030946743185 1103 72-73 0.4047887578337992 1103 74-75 0.3729833523632493 1103 76-77 0.3335359079580513 1103 78-79 0.30261374092081184 1103 80-81 0.268683830602761 1103 82-83 0.22906182560350175 1103 84-85 0.2812844414155009 1103 86-87 0.26456001059201384 1103 88-89 0.27273325710473273 1103 90-91 0.2971335185222017 1103 92-93 0.3268973242952171 1103 94-95 0.3219617278049327 1103 96-97 0.30289398614065277 1103 98-99 0.38106995932682963 1103 100-101 0.33066985744552824 1104 1 0.257497568497989 1104 2 0.2465656341408078 1104 3 0.22296126037491604 1104 4 0.16070520156282697 1104 5 0.18056344185254147 1104 6 0.22344643376900564 1104 7 0.21355730658704886 1104 8 0.23018859906819955 1104 9 0.3136676476210596 1104 10-11 0.26445670310616975 1104 12-13 0.28039482601483456 1104 14-15 0.34497756033262306 1104 16-17 0.3338612452262453 1104 18-19 0.3920234648362495 1104 20-21 0.3493516794164151 1104 22-23 0.40505532364886676 1104 24-25 0.39317610039930173 1104 26-27 0.4107347446004823 1104 28-29 0.39031272812921713 1104 30-31 0.38778847338256384 1104 32-33 0.42776399070120874 1104 34-35 0.35691128414206474 1104 36-37 0.404606894646804 1104 38-39 0.37885954752338336 1104 40-41 0.367971947187435 1104 42-43 0.3068446166973118 1104 44-45 0.42117517224369294 1104 46-47 0.39011449951450317 1104 48-49 0.3524366904171288 1104 50-51 0.38619329598456886 1104 52-53 0.4123037995745804 1104 54-55 0.43035911670367 1104 56-57 0.434888871385688 1104 58-59 0.33431092375602844 1104 60-61 0.32130215080106694 1104 62-63 0.3321094622111218 1104 64-65 0.27637531947593175 1104 66-67 0.2441187792607522 1104 68-69 0.2294851104460065 1104 70-71 0.19276407290031017 1104 72-73 0.1885270086598183 1104 74-75 0.1928471156175675 1104 76-77 0.17531901346180234 1104 78-79 0.15292171418227696 1104 80-81 0.13165448020169634 1104 82-83 0.166199205178863 1104 84-85 0.15012763269259466 1104 86-87 0.18591457144533052 1104 88-89 0.1410272201162428 1104 90-91 0.2064767195825823 1104 92-93 0.19697247954178465 1104 94-95 0.20008848173017668 1104 96-97 0.1344153255321956 1104 98-99 0.18108664723511936 1104 100-101 0.20592282840427245 1105 1 0.1563965588298899 1105 2 0.17800723998615098 1105 3 0.13589812600434925 1105 4 0.10754605602574685 1105 5 0.15098034837910035 1105 6 0.15175293688612612 1105 7 0.17645732064583797 1105 8 0.1838544861699205 1105 9 0.1510150829303214 1105 10-11 0.15914154993513563 1105 12-13 0.17544000246891756 1105 14-15 0.26975132091040877 1105 16-17 0.21925432972868464 1105 18-19 0.24567392135740818 1105 20-21 0.21174406714818872 1105 22-23 0.26946583001542734 1105 24-25 0.2600390300894233 1105 26-27 0.27639573271609663 1105 28-29 0.1813742863240151 1105 30-31 0.19762317947748897 1105 32-33 0.24267418894317672 1105 34-35 0.24297012392474926 1105 36-37 0.2555671296916415 1105 38-39 0.286082268675834 1105 40-41 0.3091839880404095 1105 42-43 0.22204403889065105 1105 44-45 0.2635261660834729 1105 46-47 0.177900931188276 1105 48-49 0.2101371304930666 1105 50-51 0.25020507485047006 1105 52-53 0.18665632397210885 1105 54-55 0.17246109051924918 1105 56-57 0.1393434389187007 1105 58-59 0.12855830834973858 1105 60-61 0.0987557267508592 1105 62-63 0.03469459583295986 1105 64-65 0.012458283238331092 1105 66-67 0.09962927822452627 1105 68-69 0.08053153676900138 1105 70-71 0.07263223437707467 1105 72-73 0.036633138261109366 1105 74-75 -0.024642522110383425 1105 76-77 0.024117867361724166 1105 78-79 -0.004936461168213668 1105 80-81 0.024719550334950213 1105 82-83 0.011943096341077819 1105 84-85 0.02056123606514859 1105 86-87 0.023569563144796746 1105 88-89 0.04740010671113026 1105 90-91 7.897308169546591E-4 1105 92-93 0.02616732873119787 1105 94-95 -0.06218428044602575 1105 96-97 0.006545306541461571 1105 98-99 -0.0029190365108675564 1105 100-101 -0.045246673235869395 1106 1 0.09938112483148842 1106 2 0.13826255461933812 1106 3 0.0651106679736202 1106 4 0.09130416372268968 1106 5 0.05688659963134057 1106 6 0.07689966896172251 1106 7 0.09702903692350162 1106 8 0.09338629736536319 1106 9 0.17768187031730776 1106 10-11 0.12686075402289276 1106 12-13 0.1506122090607036 1106 14-15 0.1799725178315441 1106 16-17 0.20924890616278446 1106 18-19 0.19569493364597434 1106 20-21 0.15866119245727361 1106 22-23 0.21445727656976032 1106 24-25 0.19123222280317265 1106 26-27 0.14667505457632046 1106 28-29 0.17988071205820688 1106 30-31 0.1768074327565614 1106 32-33 0.20008439893712193 1106 34-35 0.14068110860186067 1106 36-37 0.14267703713898072 1106 38-39 0.12581439533685312 1106 40-41 0.22672536350879824 1106 42-43 0.20424471221931384 1106 44-45 0.15790463781981856 1106 46-47 0.11224904839999539 1106 48-49 0.16727022098261557 1106 50-51 0.1899472415236474 1106 52-53 0.03485070821439962 1106 54-55 0.06930021059072544 1106 56-57 0.06612224970849923 1106 58-59 0.05339041703544467 1106 60-61 -0.020306176481497573 1106 62-63 0.0432119123099568 1106 64-65 -0.03930744424950916 1106 66-67 0.048185632240731024 1106 68-69 0.059516901736877514 1106 70-71 0.03884641630980212 1106 72-73 0.027213492678278328 1106 74-75 -0.0049924217481844835 1106 76-77 0.056044940088575856 1106 78-79 0.05250942610530274 1106 80-81 0.017530686740443002 1106 82-83 -0.0012179232690883168 1106 84-85 0.04608124359405252 1106 86-87 0.04915915867103848 1106 88-89 0.0024723954306580254 1106 90-91 0.054910760777460155 1106 92-93 0.10232836591216454 1106 94-95 0.06410542816545473 1106 96-97 0.1074774270211094 1106 98-99 0.1471829294985838 1106 100-101 0.09297024272774124 1107 1 0.013441462623070777 1107 2 0.07473406989341669 1107 3 0.052084347168950273 1107 4 0.08597837221955018 1107 5 0.09832966845250724 1107 6 0.11212501193040225 1107 7 0.11618310263526865 1107 8 0.06685176235406232 1107 9 0.15371003610149359 1107 10-11 0.04110691278311407 1107 12-13 0.10536460632129518 1107 14-15 0.11729555241806366 1107 16-17 0.03721652121254948 1107 18-19 0.03423627054263534 1107 20-21 -5.914412139702563E-4 1107 22-23 0.10780183665224286 1107 24-25 0.11143911125168415 1107 26-27 0.008450123633110707 1107 28-29 0.07029780437427746 1107 30-31 0.11288520067471097 1107 32-33 0.1401339994960722 1107 34-35 0.03415441876979486 1107 36-37 0.0430057282556362 1107 38-39 0.08429185095985758 1107 40-41 0.1175683099823388 1107 42-43 0.13159466706346024 1107 44-45 0.12698503980295328 1107 46-47 0.13106442948733132 1107 48-49 0.14979374155758762 1107 50-51 0.2107409512680789 1107 52-53 0.1538171023079471 1107 54-55 0.09937737716587947 1107 56-57 0.20592333559092424 1107 58-59 0.05328401649691017 1107 60-61 0.08142087333735759 1107 62-63 -0.1611835458337083 1107 64-65 -0.022626641770045808 1107 66-67 -0.012636738159464755 1107 68-69 0.016772975883817764 1107 70-71 0.08196815818214276 1107 72-73 -0.04271523919992859 1107 74-75 0.04824493138504238 1107 76-77 0.009062861306659897 1107 78-79 -0.06395125144980085 1107 80-81 -0.06283141687010385 1107 82-83 -0.009289488124828438 1107 84-85 0.04883219089038704 1107 86-87 -0.14006239038723578 1107 88-89 -0.10673168822059154 1107 90-91 -0.04156751606144837 1107 92-93 -0.045564429343620816 1107 94-95 -0.05102602623091457 1107 96-97 -0.023186970355411063 1107 98-99 -0.05008148753419306 1107 100-101 -0.06257892611796123 1108 1 -0.015383534966723289 1108 2 0.03937779097488203 1108 3 0.010873503571495746 1108 4 -0.023678899578015944 1108 5 0.0037783417677701436 1108 6 0.003714399712769989 1108 7 -0.0035442951420492363 1108 8 -0.04162390968394902 1108 9 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90-91 -0.2539923411480771 2214 92-93 -0.24468266418349316 2214 94-95 -0.22224820792351352 2214 96-97 -0.21384638308111192 2214 98-99 -0.3075978341245964 2214 100-101 -0.556908803222651 2215 1 -0.5585327450505453 2215 2 -0.998440308661035 2215 3 -0.5935902316364867 2215 4 -0.3495101066739963 2215 5 -0.31632736836697006 2215 6 -0.3371380644586708 2215 7 -0.37266959274293754 2215 8 -0.4315529048288056 2215 9 -0.45743192849413106 2215 10-11 -0.4926680852143832 2215 12-13 -0.41541944302054645 2215 14-15 -0.5465284403195056 2215 16-17 -0.4971091669833214 2215 18-19 -0.4844538210388123 2215 20-21 -0.4482798773873071 2215 22-23 -0.48529551202476995 2215 24-25 -0.48963289900116536 2215 26-27 -0.5589964093548971 2215 28-29 -0.5220546106525603 2215 30-31 -0.5955206680140464 2215 32-33 -0.625870058923816 2215 34-35 -0.7340937856464151 2215 36-37 -0.6384363458105256 2215 38-39 -0.6033186208314589 2215 40-41 -0.6552623043876693 2215 42-43 -0.6349527390188214 2215 44-45 -0.5650135984903741 2215 46-47 -0.6030783628123473 2215 48-49 -0.6728386243410114 2215 50-51 -0.6557336969576966 2215 52-53 -0.6433847239810007 2215 54-55 -0.6443821347557304 2215 56-57 -0.5224155157694454 2215 58-59 -0.5408786674002357 2215 60-61 -0.5310781254077384 2215 62-63 -0.4574635501057287 2215 64-65 -0.47946387777250266 2215 66-67 -0.44097425451329286 2215 68-69 -0.4459009087182153 2215 70-71 -0.4435351392840161 2215 72-73 -0.417649823879394 2215 74-75 -0.4753226065958813 2215 76-77 -0.35485858690843486 2215 78-79 -0.35378918165565665 2215 80-81 -0.3249746363718842 2215 82-83 -0.3927594371275731 2215 84-85 -0.3802295665122202 2215 86-87 -0.3590088032246612 2215 88-89 -0.3188054873822068 2215 90-91 -0.30056813016445005 2215 92-93 -0.28870983825611773 2215 94-95 -0.36416582811594367 2215 96-97 -0.39334623663582136 2215 98-99 -0.379039781929837 2215 100-101 -0.4311715622218806 2216 1 -0.4668279001405651 2216 2 -0.4817841624424801 2216 3 -0.4696020203041087 2216 4 -0.2736762418875003 2216 5 -0.31783286641771724 2216 6 -0.31098957899208557 2216 7 -0.3476792295505575 2216 8 -0.40744558322425206 2216 9 -0.5349997334623211 2216 10-11 -0.6399906560399629 2216 12-13 -0.6590716661189262 2216 14-15 -0.8239680813801655 2216 16-17 -1.1809240777878074 2216 18-19 -1.1949899518652813 2216 20-21 -1.5253964394859594 2216 22-23 -1.3170368586465955 2216 24-25 -1.3881891228075176 2216 26-27 -1.1502885418767335 2216 28-29 -1.1953489870629426 2216 30-31 -1.2110355841599656 2216 32-33 -1.03772204718193 2216 34-35 -0.644990566651181 2216 36-37 -0.9574060597710741 2216 38-39 -1.2223750751964175 2216 40-41 -1.2102367516971881 2216 42-43 -0.6569037544968594 2216 44-45 -1.0186933439702415 2216 46-47 -0.7106359237428848 2216 48-49 -0.7296264937973973 2216 50-51 -0.8809413763285434 2216 52-53 -0.9139002906000115 2216 54-55 -1.0651495099853392 2216 56-57 -1.1216349389204083 2216 58-59 -0.8464468388990554 2216 60-61 -0.8979667243989269 2216 62-63 -0.6704439188521079 2216 64-65 -0.8084566025232931 2216 66-67 -0.5697816614284932 2216 68-69 -0.606824579598559 2216 70-71 -0.4991239958881337 2216 72-73 -0.500961601663164 2216 74-75 -0.4501314981489273 2216 76-77 -0.44532609404156176 2216 78-79 -0.34202893416160407 2216 80-81 -0.3620155313351532 2216 82-83 -0.5417909767894642 2216 84-85 -0.412361704030765 2216 86-87 -0.5119555072476558 2216 88-89 -0.44367079438145396 2216 90-91 -0.4530538311762058 2216 92-93 -0.369869763644175 2216 94-95 -0.4805609994129796 2216 96-97 -0.44372457840327684 2216 98-99 -0.7368590928071939 2216 100-101 -0.5328267193315348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 16.0 12 68.0 13 341.0 14 1166.0 15 2648.0 16 4725.0 17 7830.0 18 13820.0 19 27501.0 20 30911.0 21 28440.0 22 27768.0 23 28654.0 24 32034.0 25 38520.0 26 48321.0 27 63035.0 28 81560.0 29 104557.0 30 134251.0 31 173121.0 32 224037.0 33 294374.0 34 398135.0 35 567266.0 36 941367.0 37 1748739.0 38 1857160.0 39 372468.0 40 446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.035934919916908 39.608251661076324 13.265255617493827 21.090557801512944 2 24.734302926993433 27.766476375719172 29.575920076974842 17.923300620312553 3 23.005774353916348 27.216945604877463 24.14411468247671 25.63316535872948 4 24.13167012402348 26.29740532727192 25.555788498035923 24.015136050668666 5 24.09958309889913 26.967072409595715 24.672262021080396 24.261082470424753 6 24.193030490072147 25.504547667337768 25.726337012542878 24.57608483004721 7 24.932006613836307 26.794764133573246 24.084748428951926 24.18848082363852 8 25.03339524096619 25.226149442204004 25.72200793599695 24.01844738083286 9 23.89069826212393 25.328186052128977 25.9593212945483 24.821794391198797 10-11 25.170312626882268 25.572172254782977 24.940947397721775 24.31656772061298 12-13 24.75139864328947 25.679020481633202 24.875370160171695 24.694210714905633 14-15 24.383468497489204 25.61803427112069 25.20071267077966 24.79778456061045 16-17 24.519593138496397 26.021982609520467 25.071943323840156 24.38648092814298 18-19 23.925109988216363 25.897466758818116 25.91495540567388 24.262467847291642 20-21 23.338582453535842 26.328354389787016 26.09179241554061 24.241270741136525 22-23 23.026881504732273 26.16377397416368 26.900944221487748 23.908400299616297 24-25 22.939955846176606 25.648703158943697 27.95640426902095 23.454936725858747 26-27 22.85905962728826 25.479028570963756 28.678432481446652 22.98347932030133 28-29 22.900760306714623 25.68546102805953 28.564760630982743 22.849018034243098 30-31 22.95053986639968 25.61422590288857 28.523752577456037 22.911481653255716 32-33 22.85662368287102 25.571169496376534 28.623107682652638 22.949099138099804 34-35 22.93140798802859 25.828587318921553 28.478057992805738 22.761946700244124 36-37 22.916240278963546 25.80547207796751 28.30001333879753 22.978274304271416 38-39 22.92639215714389 25.740396477398626 28.44898636147308 22.884225003984408 40-41 23.082594787387347 26.076629548938087 27.848746893212684 22.992028770461882 42-43 23.16850403297326 26.11525020123678 27.64420165438451 23.072044111405454 44-45 23.123083273544058 26.106482995489472 27.727259441695946 23.043174289270524 46-47 23.189563495589542 26.397060967592346 27.44390582925236 22.96946970756575 48-49 23.239380646972265 26.42246537203831 27.232071676277148 23.106082304712274 50-51 23.155757043291178 26.292536432718084 27.447360792911606 23.104345731079132 52-53 23.15681390233991 26.51979290427251 27.28161868590516 23.041774507482423 54-55 23.228540994722056 26.4427696898556 27.169262623597135 23.15942669182521 56-57 23.20585909568092 26.38876854086285 27.299943839119724 23.105428524336507 58-59 23.32756552753364 26.539978987181023 27.078245486831555 23.054209998453786 60-61 23.26220403511724 26.54090137430184 26.978601926737223 23.2182926638437 62-63 23.277084291668167 26.45126849696815 27.10941489052596 23.162232320837717 64-65 23.35663137263715 26.712110159615193 26.83593039651015 23.095328071237514 66-67 23.367435667265525 26.62155550043863 26.70680673963884 23.304202092657007 68-69 23.320692987813704 26.632438237860928 26.758382127686563 23.28848664663881 70-71 23.431491697853858 26.789650701168927 26.590822452571228 23.188035148405987 72-73 23.3906541226285 26.757196371899965 26.432487788616253 23.419661716855273 74-75 23.358997344351156 26.707318048347645 26.583517826156612 23.35016678114459 76-77 23.436216625764132 26.841553167052666 26.424421103541828 23.29780910364137 78-79 23.42946741829067 26.799746376531857 26.366569459962346 23.404216745215127 80-81 23.355153361757274 26.784204376643654 26.476522674240254 23.384119587358814 82-83 23.41133884452484 26.969862624636022 26.30472817215212 23.31407035868702 84-85 23.436703592954302 26.88772898436693 26.28777963731989 23.38778778535887 86-87 23.391345966198863 26.86326655270897 26.364946931224804 23.38044054986736 88-89 23.447684117943517 26.98903381469676 26.22124022584906 23.342041841510667 90-91 23.492861032244218 26.940010170606865 26.12536896368382 23.4417598334651 92-93 23.37642364541324 26.89784289245607 26.302737619367967 23.42299584276272 94-95 23.47833993425594 27.064138853613656 26.09847904651124 23.35904216561916 96-97 23.43477298781442 27.00711198918553 26.0542143550921 23.50390066790795 98-99 23.4207253565269 26.906888743835626 26.231848801530948 23.440537098106518 100-101 23.527305024018528 27.075672750735063 25.97396213691019 23.423060088336225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6764.0 1 4946.5 2 2320.5 3 1187.5 4 761.0 5 661.5 6 626.5 7 573.0 8 557.0 9 532.5 10 551.5 11 564.0 12 608.0 13 991.5 14 1251.5 15 1429.5 16 7482.5 17 8729.5 18 4287.0 19 4717.0 20 5456.0 21 6532.5 22 8282.0 23 10569.5 24 13105.5 25 16761.5 26 21552.5 27 27151.5 28 33574.5 29 42128.0 30 53289.5 31 64879.5 32 78208.5 33 96615.0 34 116354.0 35 137038.0 36 158398.0 37 175549.0 38 191474.0 39 211165.5 40 223437.0 41 225260.0 42 228925.0 43 238702.5 44 251271.5 45 265786.5 46 279143.0 47 289275.0 48 295572.0 49 295573.0 50 290467.0 51 285479.5 52 286499.0 53 285043.0 54 270637.0 55 252839.5 56 234952.0 57 208857.5 58 185615.0 59 167785.5 60 146455.0 61 123723.5 62 100944.0 63 78866.5 64 60039.5 65 43789.0 66 31456.5 67 23062.5 68 16886.0 69 12882.5 70 10174.0 71 7836.0 72 6374.0 73 4939.5 74 3465.5 75 2474.5 76 1809.0 77 1237.0 78 798.0 79 435.5 80 157.0 81 51.0 82 32.5 83 12.5 84 4.0 85 4.0 86 3.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.04189829179327032 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.7573735961349343E-5 20-21 0.0 22-23 2.7573735961349343E-5 24-25 0.0 26-27 8.272120788404803E-5 28-29 3.0331109557484276E-4 30-31 4.136060394202401E-5 32-33 4.136060394202401E-5 34-35 0.0 36-37 2.0680301971012006E-5 38-39 4.136060394202401E-5 40-41 8.961464187438536E-5 42-43 1.0340150985506004E-4 44-45 3.7224543547821615E-4 46-47 1.0340150985506004E-4 48-49 6.893433990337336E-6 50-51 1.5854898177775873E-4 52-53 0.0015372357798452258 54-55 0.0010615888345119496 56-57 1.3097524581640938E-4 58-59 0.0012063509483090336 60-61 4.4807320937192686E-4 62-63 4.067126054299028E-4 64-65 3.0331109557484276E-4 66-67 1.6544241576809605E-4 68-69 6.755565310530588E-4 70-71 4.6875351134293883E-4 72-73 8.272120788404803E-5 74-75 8.40998946821155E-4 76-77 8.341055128308175E-4 78-79 9.65080758647227E-5 80-81 5.5147471922698686E-5 82-83 0.0021921120089272725 84-85 0.0 86-87 3.446716995168668E-5 88-89 3.7913886946855347E-4 90-91 3.377782655265294E-4 92-93 3.377782655265294E-4 94-95 0.0 96-97 4.6875351134293883E-4 98-99 6.204090591303603E-5 100-101 0.008940783885467525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7253279.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.65817526650943 #Duplication Level Percentage of deduplicated Percentage of total 1 79.70554248336906 42.76853968284831 2 11.181635917837571 11.99972359691251 3 3.4260002643540877 5.514987679434579 4 1.5624925751616279 3.3536200180256923 5 0.8650513162989473 2.32085375722468 6 0.5704566918927189 1.836579909331961 7 0.39205941568732916 1.472603498928519 8 0.3063785067437425 1.3151769290197743 9 0.24653280506517455 1.1905650416818208 >10 1.5147705072178554 15.129229413301076 >50 0.15875214298990967 5.985859289452488 >100 0.0697867673264788 5.499252851964697 >500 2.0595127400410416E-4 0.07549156295760098 >1k 2.0594140466083624E-4 0.24971231654197795 >5k 5.1485350744023614E-5 0.23595451786913618 >10k+ 7.722802611603543E-5 1.0518499345051429 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 33848 0.4666579074098762 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31705 0.43711264932729044 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10596 0.14608565312322883 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 9502 0.13100281955237073 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7580 0.10450445929351401 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3786867980674671E-5 2.7573735961349343E-5 2 0.0 0.0 0.0 2.7573735961349343E-5 2.7573735961349343E-5 3 2.7573735961349343E-5 0.0 0.0 2.7573735961349343E-5 2.7573735961349343E-5 4 2.7573735961349343E-5 0.0 0.0 2.7573735961349343E-5 4.136060394202401E-5 5 2.7573735961349343E-5 0.0 0.0 2.7573735961349343E-5 4.136060394202401E-5 6 6.893433990337335E-5 0.0 0.0 2.7573735961349343E-5 4.136060394202401E-5 7 8.272120788404803E-5 0.0 0.0 4.136060394202401E-5 5.5147471922698686E-5 8 1.1029494384539737E-4 1.3786867980674671E-5 0.0 4.136060394202401E-5 5.5147471922698686E-5 9 1.930161517294454E-4 1.3786867980674671E-5 0.0 4.136060394202401E-5 5.5147471922698686E-5 10-11 3.722454354782161E-4 1.3786867980674671E-5 0.0 4.136060394202401E-5 5.5147471922698686E-5 12-13 5.928353231690109E-4 1.3786867980674671E-5 0.0 4.136060394202401E-5 6.204090591303602E-5 14-15 8.754661167728416E-4 1.3786867980674671E-5 0.0 4.136060394202401E-5 6.893433990337335E-5 16-17 0.0011029494384539739 1.3786867980674671E-5 0.0 6.204090591303602E-5 6.893433990337335E-5 18-19 0.0012683918542220698 1.3786867980674671E-5 0.0 6.893433990337335E-5 7.582777389371069E-5 20-21 0.0015027686098935391 1.3786867980674671E-5 0.0 8.272120788404803E-5 8.272120788404803E-5 22-23 0.0019163746493137794 3.4467169951686676E-5 0.0 8.272120788404803E-5 1.1029494384539737E-4 24-25 0.002488529670511778 4.136060394202401E-5 0.0 8.272120788404803E-5 1.2408181182607203E-4 26-27 0.003253700843439222 4.136060394202401E-5 0.0 8.272120788404803E-5 1.5165554778742138E-4 28-29 0.00421878160208645 4.136060394202401E-5 0.0 8.961464187438536E-5 1.5165554778742138E-4 30-31 0.005756017381931675 4.825403793236135E-5 0.0 9.65080758647227E-5 1.585489817777587E-4 32-33 0.0075138430494676965 5.5147471922698686E-5 0.0 9.65080758647227E-5 1.930161517294454E-4 34-35 0.00962323385051092 5.5147471922698686E-5 0.0 9.65080758647227E-5 2.0680301971012007E-4 36-37 0.013028590241737564 5.5147471922698686E-5 0.0 9.65080758647227E-5 2.2058988769079474E-4 38-39 0.01759204354334088 5.5147471922698686E-5 0.0 1.0340150985506004E-4 2.4816362365214406E-4 40-41 0.023423888699166267 5.5147471922698686E-5 0.0 1.2408181182607203E-4 2.4816362365214406E-4 42-43 0.03204068118708794 5.5147471922698686E-5 0.0 1.3097524581640935E-4 2.4816362365214406E-4 44-45 0.04220160288884517 5.5147471922698686E-5 0.0 1.378686798067467E-4 2.4816362365214406E-4 46-47 0.05544388958428319 6.204090591303602E-5 0.0 1.5165554778742138E-4 2.5505705764248144E-4 48-49 0.07198813116109279 6.893433990337335E-5 0.0 1.5165554778742138E-4 2.757373596134934E-4 50-51 0.09292349018974728 6.893433990337335E-5 0.0 1.585489817777587E-4 2.757373596134934E-4 52-53 0.11802248334856552 6.893433990337335E-5 0.0 1.6544241576809605E-4 3.0331109557484276E-4 54-55 0.1470645207498567 6.893433990337335E-5 0.0 1.6544241576809605E-4 3.239913975458548E-4 56-57 0.18074583922664494 6.893433990337335E-5 0.0 1.7922928374877072E-4 3.377782655265294E-4 58-59 0.2208931987863696 6.893433990337335E-5 0.0 1.7922928374877072E-4 4.067126054299028E-4 60-61 0.26709988682360075 6.893433990337335E-5 0.0 1.7922928374877072E-4 4.480732093719268E-4 62-63 0.3207376967024156 6.893433990337335E-5 0.0 1.7922928374877072E-4 4.963272473042881E-4 64-65 0.3810276703819059 6.893433990337335E-5 0.0 1.930161517294454E-4 5.239009832656375E-4 66-67 0.44972074009561747 6.893433990337335E-5 0.0 1.930161517294454E-4 5.652615872076615E-4 68-69 0.5291330996643036 6.893433990337335E-5 0.0 1.9990958571978275E-4 5.928353231690109E-4 70-71 0.6160937694524091 6.893433990337335E-5 0.0 2.0680301971012007E-4 5.928353231690109E-4 72-73 0.7149042522699044 6.893433990337335E-5 0.0 2.0680301971012007E-4 5.928353231690109E-4 74-75 0.8258471789103935 8.961464187438536E-5 0.0 2.0680301971012007E-4 5.928353231690109E-4 76-77 0.9514800133842914 9.65080758647227E-5 0.0 2.0680301971012007E-4 6.066221911496855E-4 78-79 1.0880527276008547 9.65080758647227E-5 0.0 2.0680301971012007E-4 6.341959271110349E-4 80-81 1.2369853689620929 9.65080758647227E-5 0.0 2.0680301971012007E-4 6.410893611013722E-4 82-83 1.4028827513735513 9.65080758647227E-5 0.0 2.0680301971012007E-4 6.686630970627215E-4 84-85 1.5826290426715972 1.378686798067467E-4 0.0 2.136964537004574E-4 6.893433990337336E-4 86-87 1.781008286045525 1.378686798067467E-4 0.0 2.274833216811321E-4 7.031302670144082E-4 88-89 1.994387641782427 1.378686798067467E-4 0.0 2.3437675567146942E-4 7.238105689854203E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 7385 0.0 72.9625 1 ATATCTC 7305 0.0 71.74513 1 TCGACGG 8715 0.0 63.463314 2 CGACGGC 9760 0.0 56.47358 3 TATCTCG 9335 0.0 56.24515 2 ACGGCGC 11565 0.0 47.97033 5 GACGGCG 11585 0.0 47.700268 4 CGGCGCG 11935 0.0 46.443386 6 ATCTCGA 13920 0.0 37.54833 3 TCTCGAG 13305 0.0 35.962654 4 GGTCGAC 620 0.0 34.4872 1 CTCGAGG 15030 0.0 31.760149 5 TCGAGGG 15300 0.0 31.261766 6 CGAGGGC 15490 0.0 30.816982 7 AGGGCGC 16540 0.0 28.918076 9 GGGCGCG 16375 0.0 28.252254 8 GAGGGCG 17500 0.0 27.41314 8 CGCGCCG 27455 0.0 24.549147 9 GGCGCGC 28620 0.0 23.632835 7 GCGCGCC 28625 0.0 23.59552 8 >>END_MODULE