FastQCFastQC Report
Tue 31 May 2016
SRR2048252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907180
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT473371.2115387568527685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166470.42606176321541367No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.11665702629517964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG125050.087.6689152
ATATCTC126800.086.8777161
GTCGACG101550.086.8678051
ATCTCGA132450.081.7604143
TCTCGAG102650.078.7518544
CTCGAGG104600.077.192925
CGAGGGC105050.076.68147
TCGAGGG106200.076.029946
AGGGCGC108350.074.214419
CTCGACG36900.073.88325
GAGGGCG109800.073.27768
TCTCGAC40550.067.232814
TCGACGG133000.066.6785662
CGACGGC137300.064.550753
GACGGCG147250.060.124414
ACGGCGC147800.059.83645
CGGCGCG159400.058.8191876
GGGCGCG111550.036.46850610-11
TCGCCGG15000.035.4666632
GGCGCGC270050.035.4221957
Produced by FastQC (version 0.11.5)