##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3907180 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.308309061778573 31.0 30.0 34.0 28.0 34.0 2 30.756004330489 31.0 30.0 34.0 27.0 34.0 3 31.38860891998833 31.0 31.0 34.0 28.0 34.0 4 34.26472161507788 37.0 35.0 37.0 30.0 37.0 5 34.256259757676894 35.0 35.0 37.0 30.0 37.0 6 34.023509794788055 36.0 35.0 37.0 29.0 37.0 7 34.094934709944255 36.0 35.0 37.0 30.0 37.0 8 33.824834023515685 35.0 35.0 37.0 28.0 37.0 9 36.06742996227458 39.0 35.0 39.0 31.0 39.0 10-11 35.177321751237464 38.0 34.5 39.0 27.5 39.0 12-13 34.87470183098808 37.0 34.0 39.0 27.0 39.0 14-15 35.85962202918729 39.0 34.0 40.0 27.0 41.0 16-17 35.83845945157377 39.0 34.0 40.5 27.0 41.0 18-19 35.852847322109554 39.0 34.0 40.5 27.0 41.0 20-21 36.128207428375454 39.0 34.5 41.0 28.0 41.0 22-23 35.87753008563721 39.0 34.0 40.5 27.0 41.0 24-25 35.63992918166043 39.0 34.0 40.5 26.0 41.0 26-27 35.178749635286835 38.0 33.0 40.0 25.0 41.0 28-29 35.1230498722864 38.0 33.0 40.0 25.0 41.0 30-31 34.868150302775916 38.0 33.0 40.0 24.5 41.0 32-33 34.68479734744752 38.0 33.0 40.0 24.5 41.0 34-35 34.71305442800178 38.0 33.0 40.0 25.0 41.0 36-37 34.38650126177959 37.5 32.5 40.0 23.5 41.0 38-39 34.54424725761291 38.0 33.0 40.0 24.0 41.0 40-41 34.45608392753853 38.0 32.0 40.0 23.0 41.0 42-43 34.27429680741609 38.0 32.0 40.0 23.0 41.0 44-45 34.00859648134972 37.5 31.0 40.0 22.0 41.0 46-47 33.93780335177801 37.0 31.0 40.0 22.0 41.0 48-49 33.90691150650853 37.0 31.0 40.0 22.0 41.0 50-51 33.84491244324551 37.0 31.0 40.0 23.0 41.0 52-53 33.680129018883186 37.0 31.0 40.0 22.0 41.0 54-55 33.496176782231686 36.5 31.0 40.0 22.0 41.0 56-57 33.851132914275766 37.0 31.0 40.0 23.0 41.0 58-59 33.81271940376435 36.5 31.0 40.0 23.0 41.0 60-61 33.73361644459687 36.0 31.0 40.0 23.0 41.0 62-63 33.55378431503028 36.0 31.0 40.0 23.0 41.0 64-65 33.29201150190163 35.0 31.0 39.0 23.0 41.0 66-67 32.97200372135402 35.0 31.0 39.0 22.5 41.0 68-69 32.72976801683055 35.0 31.0 39.0 22.0 40.5 70-71 32.462320522729954 35.0 31.0 38.0 22.5 40.0 72-73 32.1861508811982 35.0 31.0 37.0 22.0 40.0 74-75 31.878920474613405 35.0 30.5 37.0 21.0 39.0 76-77 31.632024375636647 35.0 30.0 36.0 20.5 39.0 78-79 31.460837611781386 35.0 30.0 36.0 22.0 38.5 80-81 31.254596921564914 34.5 30.0 35.5 21.5 37.0 82-83 31.126688941896713 34.0 30.0 35.0 23.0 37.0 84-85 31.02519246617765 34.0 30.0 35.0 23.0 36.5 86-87 30.884999667279217 34.0 30.0 35.0 23.0 36.0 88-89 30.776329859387076 34.0 30.0 35.0 23.0 36.0 90-91 30.742276014926368 34.0 30.5 35.0 23.0 36.0 92-93 30.71477280289109 34.0 31.0 35.0 24.0 35.5 94-95 30.623733485531766 34.0 31.0 35.0 23.0 35.0 96-97 30.534059603089695 34.0 31.0 35.0 23.0 35.0 98-99 30.406294693359406 34.0 31.0 35.0 22.5 35.0 100-101 29.574801007376166 33.5 30.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17706231984378817 1101 2 0.27680621623442647 1101 3 0.29637267445678717 1101 4 -0.008119627403694096 1101 5 0.047065105352658065 1101 6 -0.012004041006150601 1101 7 -0.154476296531314 1101 8 0.17496835978670333 1101 9 0.841554997330455 1101 10-11 0.4228697223935072 1101 12-13 0.1778862071397853 1101 14-15 0.03548680183770614 1101 16-17 -0.04306376832433756 1101 18-19 0.05238203386903706 1101 20-21 0.9167229958570289 1101 22-23 0.3125321208966696 1101 24-25 0.15842907207403556 1101 26-27 0.8624835316722255 1101 28-29 0.8715043989305471 1101 30-31 0.8317587767831398 1101 32-33 0.8063772228467627 1101 34-35 0.7875448599668218 1101 36-37 0.844496881641426 1101 38-39 0.8423225431076133 1101 40-41 0.6850809989060807 1101 42-43 0.6223200470133676 1101 44-45 0.7223341974064539 1101 46-47 0.6805752555590558 1101 48-49 0.6499368714879239 1101 50-51 0.43393658872489027 1101 52-53 0.3794016941593483 1101 54-55 0.5218921692604148 1101 56-57 0.6637897474502665 1101 58-59 0.6946942111892973 1101 60-61 0.799474681262982 1101 62-63 0.6480832352623409 1101 64-65 0.7802287680830879 1101 66-67 1.410962529339237 1101 68-69 1.3251758726239196 1101 70-71 1.936241062670554 1101 72-73 1.3210726647047224 1101 74-75 0.9164539815574493 1101 76-77 1.2797212314047108 1101 78-79 1.537101391385196 1101 80-81 2.203554988008541 1101 82-83 2.0992660798303895 1101 84-85 2.035150026164434 1101 86-87 1.8921419144755163 1101 88-89 1.8809254841711862 1101 90-91 1.760064800104118 1101 92-93 1.6776757217605507 1101 94-95 1.6293582959835895 1101 96-97 1.7103654571177138 1101 98-99 1.7078692921163032 1101 100-101 1.866812364036626 1102 1 1.3530571921889951 1102 2 2.45088140103298 1102 3 1.403959464361911 1102 4 2.29759249930607 1102 5 2.0940643043566496 1102 6 2.567066872969505 1102 7 2.310604998473586 1102 8 2.6763904944655224 1102 9 2.1343952452595687 1102 10-11 3.060948649618986 1102 12-13 3.086534163390766 1102 14-15 3.6038474593390504 1102 16-17 3.388860737290237 1102 18-19 3.4944576102300786 1102 20-21 3.5047915516556216 1102 22-23 3.657589280217529 1102 24-25 3.7002498394138144 1102 26-27 4.39297556229981 1102 28-29 4.424540648601244 1102 30-31 4.379773939960394 1102 32-33 4.379517716693826 1102 34-35 4.19386927873348 1102 36-37 4.494122590048114 1102 38-39 4.6130865976357995 1102 40-41 4.53324786142808 1102 42-43 4.559734189336375 1102 44-45 4.628594938368224 1102 46-47 4.688805883018382 1102 48-49 4.532507290720297 1102 50-51 4.404131250583028 1102 52-53 4.460617310745349 1102 54-55 4.48322561379133 1102 56-57 4.434400911231052 1102 58-59 4.597035290744142 1102 60-61 4.417436801237766 1102 62-63 4.2894462194663845 1102 64-65 4.363518261058569 1102 66-67 4.32454278591225 1102 68-69 4.180505092298748 1102 70-71 4.108140986269632 1102 72-73 3.951179907837197 1102 74-75 3.9341529971476668 1102 76-77 3.827456841727841 1102 78-79 3.622350057125356 1102 80-81 3.507167151945197 1102 82-83 3.3180706015057844 1102 84-85 3.0792255408214935 1102 86-87 2.976787570430254 1102 88-89 2.927445161386416 1102 90-91 2.791435570213462 1102 92-93 2.6505959928277676 1102 94-95 2.612426010242906 1102 96-97 2.5920881133374998 1102 98-99 2.5672055901455018 1102 100-101 2.5480632248028243 1103 1 1.2847792082686844 1103 2 2.3426149411810826 1103 3 1.3999545762351602 1103 4 2.2376538332922777 1103 5 2.031407270621038 1103 6 2.3341981300957855 1103 7 2.244895286101084 1103 8 2.599280634737177 1103 9 2.050905548452725 1103 10-11 3.015820751488768 1103 12-13 3.118835777202179 1103 14-15 3.3993775585905865 1103 16-17 3.3108219959843126 1103 18-19 3.3963404271642545 1103 20-21 3.4809481204360253 1103 22-23 3.5819000145806754 1103 24-25 3.607493960887588 1103 26-27 4.358095843328343 1103 28-29 4.399909954176117 1103 30-31 4.405136979299137 1103 32-33 4.291328087580197 1103 34-35 4.315992709883524 1103 36-37 4.367433499661715 1103 38-39 4.444811870850316 1103 40-41 4.416088673812382 1103 42-43 4.452850033898436 1103 44-45 4.530312832944148 1103 46-47 4.463976146072852 1103 48-49 4.244214690208054 1103 50-51 4.0749177993562995 1103 52-53 4.19142860353486 1103 54-55 4.074971788496224 1103 56-57 4.179822718724907 1103 58-59 4.3397562922470705 1103 60-61 4.188239576667552 1103 62-63 4.153506467400703 1103 64-65 4.183989822403472 1103 66-67 4.109250408693988 1103 68-69 3.9216939484250553 1103 70-71 3.838647676340031 1103 72-73 3.7009006069306203 1103 74-75 3.7265053961032315 1103 76-77 3.620265853661639 1103 78-79 3.3420262156177962 1103 80-81 3.2857801124036854 1103 82-83 3.1761907654401575 1103 84-85 2.9229247243541288 1103 86-87 2.838321481231368 1103 88-89 2.8072519175868607 1103 90-91 2.651585182761959 1103 92-93 2.4860158889481383 1103 94-95 2.5441102470677492 1103 96-97 2.5470745658418608 1103 98-99 2.491967562097873 1103 100-101 2.593995501249605 1104 1 -0.2613605445300564 1104 2 -0.37340930623350843 1104 3 -0.2208649267000773 1104 4 -0.6458542477045768 1104 5 -0.6626312939411676 1104 6 -1.1755483317657038 1104 7 -0.9008192267158748 1104 8 -0.667026191046709 1104 9 0.1603202616319308 1104 10-11 -0.7017385864002321 1104 12-13 -1.1786444819571642 1104 14-15 -1.474112483596116 1104 16-17 -1.3295586609909051 1104 18-19 -1.2501681995284812 1104 20-21 -0.7547214965145983 1104 22-23 -1.260902687882485 1104 24-25 -1.4603307016430378 1104 26-27 -1.3627860042494149 1104 28-29 -1.1214805007361761 1104 30-31 -1.3392903526390683 1104 32-33 -1.3919577676530324 1104 34-35 -1.1000406153529312 1104 36-37 -1.2145951310160719 1104 38-39 -1.2853041429309613 1104 40-41 -1.1768345774322526 1104 42-43 -1.206300816280887 1104 44-45 -1.2741438447519684 1104 46-47 -1.6084156458750094 1104 48-49 -1.6540844842967672 1104 50-51 -1.7646576445538287 1104 52-53 -1.58673973838998 1104 54-55 -1.5286837017601727 1104 56-57 -1.434174962397556 1104 58-59 -1.0030973476979703 1104 60-61 -1.0770517625086633 1104 62-63 -1.1283464216319956 1104 64-65 -1.0727563386726722 1104 66-67 -0.7923940433807601 1104 68-69 -0.7464301355740801 1104 70-71 -0.8781217883896453 1104 72-73 -0.8629541217175394 1104 74-75 -0.7972506942133215 1104 76-77 -0.8619762011544267 1104 78-79 -0.873956700087664 1104 80-81 -0.6777011105593012 1104 82-83 -0.783191884500706 1104 84-85 -0.6717215287663407 1104 86-87 -0.7336221146488313 1104 88-89 -0.6165826760906619 1104 90-91 -0.40804474127812895 1104 92-93 -0.17609088725725286 1104 94-95 -0.20600896961546056 1104 96-97 0.04101423982926633 1104 98-99 0.14341431368217528 1104 100-101 0.2948264836943082 1105 1 -0.9363235461079178 1105 2 -1.5041977648510105 1105 3 -0.9216406694865071 1105 4 -1.8585681410886963 1105 5 -1.6011676586552852 1105 6 -1.889161167525213 1105 7 -1.505897141652948 1105 8 -2.0414045935941267 1105 9 -1.301597876585383 1105 10-11 -2.1325829463363846 1105 12-13 -2.562784727802125 1105 14-15 -2.739041813119904 1105 16-17 -2.8011471231087484 1105 18-19 -2.6235733815786375 1105 20-21 -2.452205770492313 1105 22-23 -2.977347416104781 1105 24-25 -2.8195813176930713 1105 26-27 -3.9742403261214108 1105 28-29 -3.2332613650668165 1105 30-31 -3.1961653189118024 1105 32-33 -3.127120843029804 1105 34-35 -3.1268680915490314 1105 36-37 -3.3852995897244185 1105 38-39 -3.1670617070586076 1105 40-41 -3.2774140301243833 1105 42-43 -3.6764085601865943 1105 44-45 -3.5355896553721315 1105 46-47 -3.9364507509863635 1105 48-49 -3.744856255860185 1105 50-51 -3.6437281741828826 1105 52-53 -3.498471545823584 1105 54-55 -3.1527637416974237 1105 56-57 -2.607139278271056 1105 58-59 -2.717493489486049 1105 60-61 -2.8812452052402335 1105 62-63 -3.1234246365985037 1105 64-65 -2.737394420867819 1105 66-67 -2.832782433574881 1105 68-69 -2.6198868330958582 1105 70-71 -2.5365662940901004 1105 72-73 -2.4127688535381004 1105 74-75 -2.1541114924291485 1105 76-77 -2.4670193210105644 1105 78-79 -2.4996209579763047 1105 80-81 -2.345485022272303 1105 82-83 -2.4705898041715706 1105 84-85 -2.3817810471065783 1105 86-87 -2.221433054013925 1105 88-89 -2.249497437101585 1105 90-91 -2.356748145299651 1105 92-93 -2.3745916672296197 1105 94-95 -2.3254864745956354 1105 96-97 -2.272024185399541 1105 98-99 -2.327318335292336 1105 100-101 -2.430107358772318 1106 1 0.9259418376296615 1106 2 1.9039603068774902 1106 3 0.9910047254403516 1106 4 1.7310147251833143 1106 5 1.4557395976093943 1106 6 1.8309701347730325 1106 7 1.813144620193242 1106 8 2.2447343392237755 1106 9 1.7547721329275205 1106 10-11 2.501792362933948 1106 12-13 2.4483954578913583 1106 14-15 2.520946230110283 1106 16-17 2.3681961836248107 1106 18-19 2.557683523354946 1106 20-21 2.865269442437153 1106 22-23 2.7587652097998046 1106 24-25 2.766854565138665 1106 26-27 3.515126328628881 1106 28-29 3.773196451974407 1106 30-31 3.7376500721977735 1106 32-33 3.6405545246105717 1106 34-35 3.8573114054183506 1106 36-37 3.952985098541184 1106 38-39 3.9741720821265716 1106 40-41 3.7972799311009453 1106 42-43 3.9416993819765764 1106 44-45 3.918258374487479 1106 46-47 3.9829498654335964 1106 48-49 3.866058689496164 1106 50-51 3.8312370865534646 1106 52-53 3.895819349055806 1106 54-55 3.8778830549183247 1106 56-57 3.852523286334886 1106 58-59 4.0098525216587895 1106 60-61 3.9110819113806876 1106 62-63 3.9444901189037296 1106 64-65 3.940104786390261 1106 66-67 3.868933776936977 1106 68-69 3.7139708712447295 1106 70-71 3.6714900845032403 1106 72-73 3.4911175005963777 1106 74-75 3.5184871054780125 1106 76-77 3.4414818514395797 1106 78-79 3.2593180907737924 1106 80-81 3.050842259966032 1106 82-83 2.913301276118542 1106 84-85 2.8018709987734916 1106 86-87 2.645281530619606 1106 88-89 2.619314631560968 1106 90-91 2.4552326592374563 1106 92-93 2.346118538640912 1106 94-95 2.3993838590830663 1106 96-97 2.4425215684323796 1106 98-99 2.4132674757381842 1106 100-101 2.377548205468056 1107 1 -0.9050451999330207 1107 2 -2.32576603566066 1107 3 -0.9535035805218293 1107 4 -1.7813926116373047 1107 5 -1.9477905376919615 1107 6 -1.9386395052794114 1107 7 -2.126619974375778 1107 8 -1.8490138732034076 1107 9 -0.24557867739377315 1107 10-11 -1.6872977793960402 1107 12-13 -2.056878618585671 1107 14-15 -2.6342083324243575 1107 16-17 -2.8865332542190174 1107 18-19 -2.9110515509188772 1107 20-21 -1.9569956319718358 1107 22-23 -2.5753197962150907 1107 24-25 -2.8284161457003165 1107 26-27 -2.9861512351357717 1107 28-29 -3.0076849261800334 1107 30-31 -3.3144806740547423 1107 32-33 -3.2004938378510737 1107 34-35 -3.4960506101798288 1107 36-37 -3.0768834388255044 1107 38-39 -3.3414685109826543 1107 40-41 -3.660610611320962 1107 42-43 -3.935459884341366 1107 44-45 -4.126507679876362 1107 46-47 -4.145919320730798 1107 48-49 -3.974411053552913 1107 50-51 -4.229878399436558 1107 52-53 -4.429669979573738 1107 54-55 -4.610122000297061 1107 56-57 -4.377906488072746 1107 58-59 -4.194447722992006 1107 60-61 -3.840152872285831 1107 62-63 -3.8052387917919717 1107 64-65 -3.991951618459499 1107 66-67 -3.6918317367621114 1107 68-69 -3.5521653897882075 1107 70-71 -3.5487549177270026 1107 72-73 -3.589902868947977 1107 74-75 -3.5739861924417795 1107 76-77 -3.4476064120804146 1107 78-79 -3.1425174254934625 1107 80-81 -2.808394794492422 1107 82-83 -2.9401818213497997 1107 84-85 -2.8747849627311837 1107 86-87 -2.7272681767455964 1107 88-89 -2.357450449606322 1107 90-91 -2.0186837269593703 1107 92-93 -1.6824971027902293 1107 94-95 -1.0045095894118532 1107 96-97 -0.5237573334775156 1107 98-99 0.031123077700797808 1107 100-101 0.3383620951454134 1108 1 -0.2644967440341439 1108 2 -0.8839867087640876 1108 3 -0.40513499766871064 1108 4 -1.022814159936317 1108 5 -0.11673161040018698 1108 6 -1.0065253397470215 1108 7 -0.3760977658638254 1108 8 -1.048528682411618 1108 9 0.19082124967326308 1108 10-11 -0.4586713530689437 1108 12-13 0.3810036625200297 1108 14-15 -1.3194885943045875 1108 16-17 -0.18188315011992273 1108 18-19 -1.0347261342045115 1108 20-21 -0.03668799054760541 1108 22-23 -0.725884238002287 1108 24-25 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2215 74-75 -5.101215562492978 2215 76-77 -4.72640265125532 2215 78-79 -4.931554069158462 2215 80-81 -5.305801860349259 2215 82-83 -5.24857128086369 2215 84-85 -5.523961079159886 2215 86-87 -5.371540656367397 2215 88-89 -4.854553808697279 2215 90-91 -3.7994019512732926 2215 92-93 -2.66540334715906 2215 94-95 -2.2381169499443914 2215 96-97 -1.8108416976458024 2215 98-99 -1.2441455965765549 2215 100-101 -1.1049370854865472 2216 1 -0.10548160013412655 2216 2 -0.11590580438866738 2216 3 -0.12318482737954639 2216 4 -0.21408441656440402 2216 5 0.18841861263484105 2216 6 0.5600432498739281 2216 7 -0.17670946658292053 2216 8 0.21064022433304075 2216 9 0.4962674764523811 2216 10-11 -0.0976507205725099 2216 12-13 -0.39019385237149606 2216 14-15 -0.6088840488647804 2216 16-17 -1.024329131544306 2216 18-19 -0.8593894091390908 2216 20-21 0.13191638411664997 2216 22-23 -0.21122326529503255 2216 24-25 -0.2981376137747205 2216 26-27 0.128179584371523 2216 28-29 0.13766751223122498 2216 30-31 0.43282048230523884 2216 32-33 0.27267760508507877 2216 34-35 -0.018591585544335487 2216 36-37 0.03774300662446706 2216 38-39 -0.11641873722699358 2216 40-41 -0.03198919352307428 2216 42-43 0.15113740072651183 2216 44-45 0.5628819631603363 2216 46-47 0.035227989475338006 2216 48-49 -0.24911492484502418 2216 50-51 -0.281896498984068 2216 52-53 -0.586318546692894 2216 54-55 -0.7103914824488946 2216 56-57 -0.28869180732865374 2216 58-59 -0.27492032228813557 2216 60-61 0.0408546691922993 2216 62-63 0.17053395833372065 2216 64-65 1.664022237749645 2216 66-67 0.31732864533944394 2216 68-69 -0.3151558372492431 2216 70-71 0.17103794300703612 2216 72-73 0.5149789812279906 2216 74-75 0.6062330131891898 2216 76-77 0.7720949453856498 2216 78-79 1.9587827253361034 2216 80-81 2.1116906755930493 2216 82-83 2.1008136537633284 2216 84-85 1.9681632524523245 2216 86-87 1.8985186508059329 2216 88-89 1.7788833864922182 2216 90-91 1.698323814518762 2216 92-93 1.6849578997235994 2216 94-95 1.6577689425137372 2216 96-97 1.6563814297200672 2216 98-99 1.667101961562036 2216 100-101 1.742167022244459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 106.0 11 1175.0 12 4972.0 13 10719.0 14 14363.0 15 16056.0 16 16272.0 17 16654.0 18 18595.0 19 25864.0 20 30915.0 21 40247.0 22 56542.0 23 74062.0 24 90891.0 25 104336.0 26 113066.0 27 116035.0 28 117649.0 29 118613.0 30 119711.0 31 124298.0 32 140481.0 33 165799.0 34 209808.0 35 303748.0 36 481039.0 37 704388.0 38 575964.0 39 94765.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.303760144419524 44.21179072888296 10.514516306961617 20.969932819735902 2 21.953671090388752 24.858717583879354 36.20510528704281 16.982506038689074 3 19.752199796272503 30.6257198286232 25.059889741450352 24.56219063365394 4 22.819021089128498 23.511541191227735 28.209175980931512 25.460261738712255 5 21.71814454414693 26.911608884156863 25.245240813067227 26.125005758628983 6 20.98736172891958 22.595810789367267 29.006444545682562 27.41038293603059 7 24.047292420620497 25.38746615205852 24.771344038411335 25.79389738890965 8 25.231202673643228 21.979955865844865 27.43430168781663 25.35453977269528 9 20.468010176137266 27.67264369698862 27.26705705905538 24.592289067818733 10-11 25.089322734043478 22.60214528125144 25.729157602158075 26.579374382547005 12-13 23.872383662897537 22.861219600837433 25.547428068325495 27.718968667939535 14-15 23.599552618512583 22.621212741670462 25.978032749962885 27.801201889854067 16-17 23.786528903198725 24.22880440624696 25.566329168351597 26.418337522202712 18-19 22.42471552372811 23.334361355248543 26.99357337005206 27.24734975097129 20-21 22.165564307202782 26.858897476730668 26.215922708400196 24.759615507666354 22-23 20.856441812319883 24.219806090336498 28.910970762796406 26.012781334547213 24-25 20.731809224531407 23.44046389473532 29.78021613800953 26.047510742723745 26-27 20.745806438403143 23.474027303579565 31.6349003629216 24.145265895095697 28-29 20.869053895648523 23.24594720488946 31.96133784468594 23.92366105477608 30-31 20.87440302212849 23.342628187081218 31.84292763578847 23.94004115500182 32-33 20.877269539667996 23.17434825116836 32.02262245404614 23.9257597551175 34-35 20.94934198066124 23.657932319473378 31.851258452387654 23.54146724747772 36-37 20.881554974942897 23.379446354534114 31.50514994783316 24.233848722689828 38-39 21.10052788718819 23.439568107889592 31.54281752348465 23.91708648143757 40-41 21.514084326803477 23.788793963933067 30.153550642662996 24.543571066600464 42-43 21.51829452443962 23.962704559298523 29.800636264518143 24.718364651743713 44-45 21.711445944268327 24.137577094033283 29.462315911798925 24.68866104989947 46-47 21.730378869179322 24.536136143002263 29.21013317458071 24.523351813237706 48-49 21.69462855785643 24.79545900695722 28.897706596832535 24.612205838353812 50-51 21.638287065717627 24.66676400886215 29.2557916153562 24.439157310064026 52-53 21.67875552188535 25.012617795955137 28.971214533243927 24.337412148915586 54-55 21.719890488764403 24.958301116995152 28.83739137623334 24.48441701800711 56-57 21.71342246837873 25.080019348993392 29.160942162889857 24.04561601973802 58-59 21.83917062757045 25.464232597340207 28.767742805684044 23.9288539694053 60-61 21.74718361416464 25.672508775191925 28.64874777316988 23.931559837473554 62-63 21.804788963064457 25.55647992415997 28.868696958912604 23.77003415386297 64-65 21.956969716126356 25.91826813133825 28.512800221438567 23.611961931096825 66-67 21.948546102100185 25.80892593005476 28.350455493795156 23.892072474049904 68-69 21.966694927455087 25.784102506678614 28.29743573336746 23.95176683249884 70-71 22.10527177903763 25.945666971150168 28.05086452753571 23.898196722276495 72-73 22.03720515250642 25.984330766157505 27.829103947202395 24.14936013413368 74-75 22.128827201309793 25.933873782865618 28.013519726953934 23.923779288870655 76-77 22.233362681012906 26.178049130063112 27.796505407992466 23.792082780931516 78-79 22.193784544580048 26.260093758170054 27.715056346912135 23.831065350337756 80-81 22.279047804298752 26.197129387435442 27.83215771989005 23.69166508837576 82-83 22.37789012939774 26.568971262635078 27.56739007027578 23.485748537691396 84-85 22.403024687882308 26.549416714868524 27.672771666521633 23.37478693072753 86-87 22.462432683184865 26.497198793688813 27.799539269299384 23.240829253826934 88-89 22.584259410577328 26.665854454788057 27.495065620875618 23.254820513758993 90-91 22.604044160461108 27.0022382243676 27.376094637603448 23.017622977567846 92-93 22.56854909815639 26.901629136025296 27.642076872656705 22.88774489316161 94-95 22.79566843217858 27.137614592020604 27.356724222721958 22.709992753078854 96-97 22.706629441082605 27.318778977978813 27.29797107599248 22.6766205049461 98-99 22.84752452795516 27.170922546983732 27.49609783258707 22.48545509247404 100-101 23.01043105791443 27.468571946299864 26.96694391706751 22.554053078718194 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17315.0 1 10869.0 2 3416.0 3 1862.0 4 1110.5 5 789.0 6 611.5 7 509.0 8 480.0 9 438.5 10 395.0 11 396.0 12 422.0 13 602.0 14 750.5 15 977.0 16 10865.5 17 12037.0 18 3375.5 19 3073.5 20 3054.5 21 3415.5 22 4179.5 23 5242.0 24 6535.0 25 8374.0 26 10771.0 27 14501.0 28 19165.0 29 25519.5 30 34597.0 31 44046.0 32 54509.0 33 69215.5 34 84303.5 35 98787.0 36 111237.0 37 118918.0 38 124190.0 39 128902.5 40 130248.5 41 126834.5 42 123731.5 43 125399.5 44 128159.0 45 130054.5 46 131897.5 47 133163.5 48 133694.5 49 131546.0 50 127245.5 51 124216.5 52 124633.5 53 123365.0 54 117163.5 55 109488.5 56 102038.5 57 92991.5 58 84805.0 59 77703.0 60 69613.0 61 61859.0 62 53980.0 63 45630.5 64 38603.5 65 32704.5 66 27760.5 67 23926.5 68 20955.5 69 18659.0 70 16651.0 71 14641.0 72 12933.5 73 11243.0 74 9594.5 75 8253.5 76 6960.5 77 5633.0 78 4481.5 79 3560.0 80 2750.5 81 2060.5 82 1522.5 83 1127.5 84 803.0 85 536.5 86 382.0 87 265.5 88 173.0 89 117.5 90 77.0 91 49.5 92 36.0 93 30.0 94 17.5 95 15.0 96 23.0 97 25.5 98 23.0 99 11.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00586100461202197 2 0.007396639008184931 3 0.0 4 2.5593906602716026E-5 5 0.0 6 0.0 7 0.0 8 5.118781320543205E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002712954099887899 22-23 0.0039542585701196255 24-25 1.2796953301358013E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.1517257971222212E-4 38-39 1.2796953301358013E-5 40-41 0.0 42-43 0.0 44-45 0.01087741030615431 46-47 0.0013564770499439494 48-49 4.350964122461724E-4 50-51 3.8390859904074036E-5 52-53 0.0 54-55 2.5593906602716026E-5 56-57 0.0 58-59 8.445989178896289E-4 60-61 1.2796953301358013E-5 62-63 2.175482061230862E-4 64-65 3.8390859904074036E-5 66-67 0.015241171381917393 68-69 0.003007284025819133 70-71 4.350964122461724E-4 72-73 8.445989178896289E-4 74-75 1.2796953301358013E-5 76-77 0.0 78-79 1.2796953301358013E-5 80-81 0.0 82-83 5.118781320543205E-5 84-85 0.0 86-87 0.014319790744219617 88-89 0.00392866466351691 90-91 0.0016636039291765415 92-93 4.350964122461724E-4 94-95 8.95786731095061E-5 96-97 2.687360193285183E-4 98-99 0.0014460557230534553 100-101 5.118781320543205E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3907180.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.78158014639268 #Duplication Level Percentage of deduplicated Percentage of total 1 87.32508730249936 53.95081879970102 2 7.076257756173995 8.74364771399193 3 2.0208045284922393 3.745454908117097 4 0.9334450768953829 2.306788473218711 5 0.5567028535942928 1.7196990983530658 6 0.35886699963345364 1.3302822179849814 7 0.24664650286260392 1.0666747479103404 8 0.17982972993844376 0.8888131898316892 9 0.1326347488651498 0.7374945930488027 >10 0.9683060417535053 12.723269679169832 >50 0.1544401411259405 6.559210143049528 >100 0.04617775469078982 4.051134531781099 >500 4.6348412256719235E-4 0.19547142966384357 >1k 2.528095143333346E-4 0.31563337777935097 >5k 0.0 0.0 >10k+ 8.426983811111141E-5 1.6656070963986784 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 47337 1.2115387568527685 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16647 0.42606176321541367 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.11665702629517964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.815329726298763E-4 0.0 0.0 0.0 0.0 2 3.5831469243802433E-4 0.0 0.0 0.0 0.0 3 4.095025056434564E-4 0.0 0.0 0.0 0.0 4 5.374720386570365E-4 0.0 0.0 0.0 0.0 5 7.166293848760487E-4 0.0 0.0 0.0 0.0 6 8.957867310950609E-4 0.0 0.0 0.0 0.0 7 0.001023756264108641 0.0 0.0 0.0 2.5593906602716026E-5 8 0.0011005379839167891 0.0 0.0 0.0 2.5593906602716026E-5 9 0.0018171673687928378 0.0 0.0 0.0 2.5593906602716026E-5 10-11 0.002277857687641726 0.0 0.0 0.0 7.678171980814809E-5 12-13 0.0031096596522299973 0.0 0.0 0.0 1.4076648631493815E-4 14-15 0.004274182402653577 0.0 0.0 0.0 1.7915734621901217E-4 16-17 0.006321694930870859 0.0 0.0 0.0 2.047512528217282E-4 18-19 0.009712887555730732 0.0 0.0 1.2796953301358013E-5 2.047512528217282E-4 20-21 0.012451435562221346 0.0 0.0 2.5593906602716026E-5 2.687360193285183E-4 22-23 0.017186308283723813 0.0 0.0 2.5593906602716026E-5 3.455177391366663E-4 24-25 0.025069231517360346 0.0 0.0 2.5593906602716026E-5 3.5831469243802433E-4 26-27 0.03228671317932627 0.0 0.0 5.118781320543205E-5 3.5831469243802433E-4 28-29 0.038825956316320215 0.0 0.0 5.118781320543205E-5 4.2229945894481445E-4 30-31 0.04373998638404168 0.0 0.0 7.678171980814807E-5 4.4789336554753044E-4 32-33 0.049549803182858225 0.0 0.0 7.678171980814807E-5 4.6069031884888846E-4 34-35 0.05515486872885303 0.0 0.0 7.678171980814807E-5 4.7348727215024643E-4 36-37 0.06210361437149044 2.5593906602716026E-5 0.0 7.678171980814807E-5 4.990811787529625E-4 38-39 0.07118945121545463 2.5593906602716026E-5 0.0 7.678171980814807E-5 5.630659452597526E-4 40-41 0.08294985129940263 2.5593906602716026E-5 0.0 7.678171980814807E-5 5.758628985611106E-4 42-43 0.09808864705490916 2.5593906602716026E-5 0.0 7.678171980814807E-5 6.142537584651846E-4 44-45 0.11649066590226198 2.5593906602716026E-5 0.0 7.678171980814807E-5 6.782385249719746E-4 46-47 0.13914127324566566 2.5593906602716026E-5 0.0 7.678171980814807E-5 7.294263381774067E-4 48-49 0.16525985493373738 2.5593906602716026E-5 0.0 7.678171980814807E-5 7.550202447801227E-4 50-51 0.1946032688537513 2.5593906602716026E-5 0.0 7.678171980814807E-5 7.806141513828388E-4 52-53 0.23074186497678634 5.118781320543205E-5 0.0 7.678171980814807E-5 8.445989178896288E-4 54-55 0.27239594797270666 5.118781320543205E-5 0.0 7.678171980814807E-5 8.573958711909868E-4 56-57 0.3208196192650454 5.118781320543205E-5 0.0 1.023756264108641E-4 8.957867310950609E-4 58-59 0.3770622290245138 5.118781320543205E-5 0.0 1.023756264108641E-4 8.957867310950609E-4 60-61 0.4435807922849728 5.118781320543205E-5 0.0 1.023756264108641E-4 8.957867310950609E-4 62-63 0.5141687866952636 5.118781320543205E-5 0.0 1.023756264108641E-4 8.957867310950609E-4 64-65 0.5930236129382316 5.118781320543205E-5 0.0 1.023756264108641E-4 8.957867310950609E-4 66-67 0.6847649711556673 5.118781320543205E-5 0.0 1.023756264108641E-4 9.469745443004929E-4 68-69 0.7881131660174345 5.118781320543205E-5 0.0 1.023756264108641E-4 0.001023756264108641 70-71 0.9009822941354122 5.118781320543205E-5 0.0 1.023756264108641E-4 0.0010877410306154311 72-73 1.0273778018929254 5.118781320543205E-5 0.0 1.023756264108641E-4 0.0011261318905195052 74-75 1.1648298773028118 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.0011517257971222212 76-77 1.318290941292697 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.001177319703724937 78-79 1.488004135975307 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.001190116657026295 80-81 1.6684795683843592 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.0012029136103276531 82-83 1.865322304065848 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.0012029136103276531 84-85 2.0805926525012923 5.118781320543205E-5 0.0 1.2796953301358013E-4 0.0012285075169303692 86-87 2.311206547945065 5.118781320543205E-5 0.0 1.4076648631493815E-4 0.0012541014235330852 88-89 2.5616685179592444 5.118781320543205E-5 0.0 1.5356343961629615E-4 0.0012541014235330852 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 12505 0.0 87.668915 2 ATATCTC 12680 0.0 86.877716 1 GTCGACG 10155 0.0 86.867805 1 ATCTCGA 13245 0.0 81.760414 3 TCTCGAG 10265 0.0 78.751854 4 CTCGAGG 10460 0.0 77.19292 5 CGAGGGC 10505 0.0 76.6814 7 TCGAGGG 10620 0.0 76.02994 6 AGGGCGC 10835 0.0 74.21441 9 CTCGACG 3690 0.0 73.8832 5 GAGGGCG 10980 0.0 73.2776 8 TCTCGAC 4055 0.0 67.23281 4 TCGACGG 13300 0.0 66.678566 2 CGACGGC 13730 0.0 64.55075 3 GACGGCG 14725 0.0 60.12441 4 ACGGCGC 14780 0.0 59.8364 5 CGGCGCG 15940 0.0 58.819187 6 GGGCGCG 11155 0.0 36.468506 10-11 TCGCCGG 1500 0.0 35.466663 2 GGCGCGC 27005 0.0 35.422195 7 >>END_MODULE