FastQCFastQC Report
Tue 31 May 2016
SRR2048252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907180
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT230630.5902722679784397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164060.4198936317241591No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT150280.3846252284256164No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97650.249924497975522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG33400.071.86771
TATCTCG63850.060.269292
ATATCTC62650.059.4058841
TCGACGG48250.052.776362
CGACGGC52350.049.1874773
TCTCGAG74250.048.3643154
ATCTCGA80200.047.982473
CTCGAGG85900.043.296945
GACGGCG64750.041.4484564
TCGAGGG89400.041.283086
ACGGCGC66250.040.867455
CGAGGGC90150.040.8342447
GAGGGCG95300.038.577738
AGGGCGC95900.038.3363659
CTCGACG18450.037.8450935
CGGCGCG73800.036.8796546
TTTCTCG11750.036.793872
TCGCGAG1107.00893E-834.5516852
GGTCGAC3350.034.052991
TTCTCGA13500.032.376213
Produced by FastQC (version 0.11.5)