FastQCFastQC Report
Tue 31 May 2016
SRR2048251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737001
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT623561.6686107389321008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129900.3476049377562382No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT52650.1408883754647109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC135000.088.815091
TATCTCG135300.088.647342
GTCGACG99000.087.618691
ATCTCGA143800.082.4098053
TCTCGAG123350.080.785524
CTCGAGG127200.078.2656865
TCGAGGG127250.078.1229556
CGAGGGC127000.077.9027567
AGGGCGC128700.076.910649
GAGGGCG130850.075.719518
CTCGACG26050.071.655925
TCGACGG121850.071.495042
CGACGGC127550.068.257323
TCTCGAC29000.064.3667764
GACGGCG138050.063.0313074
ACGGCGC140100.062.00735
CGGCGCG151950.060.234916
TCGCCGG11850.039.283472
GGGCGCG134250.037.2724110-11
GATCCTT125850.034.87413420-21
Produced by FastQC (version 0.11.5)