FastQCFastQC Report
Tue 31 May 2016
SRR2048251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737001
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209700.5611451535603014No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT173560.4644365896610678No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT118940.3182766073651037No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80080.21428947971916518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC41150.073.460721
GTCGACG33050.073.200531
TATCTCG43300.071.7553562
TCGACGG41050.060.5275342
CGACGGC47200.053.3455623
ATCTCGA67550.045.784673
TCTCGAG67400.045.316614
ACGGCGC61250.043.350555
GACGGCG60650.043.232924
CTCGAGG74650.042.1864135
TCGAGGG78750.039.7487756
CGAGGGC79750.039.250367
CGGCGCG70050.038.447126
AGGGCGC85750.036.7255489
GAGGGCG85950.036.47438
CTCGACG11700.033.2902375
TTTCTCG10900.031.3812662
GTCGCGC2200.030.2322032
GGGCGCG86850.027.236328
TTCTCGA12800.025.6096463
Produced by FastQC (version 0.11.5)