##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048251_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3737001
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.745070445525705	31.0	28.0	33.0	16.0	34.0
2	30.6458157222864	31.0	30.0	33.0	27.0	34.0
3	28.439379063586014	31.0	28.0	33.0	16.0	34.0
4	32.884101716857984	35.0	33.0	37.0	19.0	37.0
5	34.147731563357894	35.0	33.0	37.0	30.0	37.0
6	34.7761207449503	35.0	35.0	37.0	32.0	37.0
7	34.97805405992666	35.0	35.0	37.0	32.0	37.0
8	35.15450544433892	35.0	35.0	37.0	33.0	37.0
9	36.883244077269445	39.0	37.0	39.0	33.0	39.0
10-11	36.9139937345481	39.0	37.0	39.0	33.0	39.0
12-13	36.867106805697944	39.0	37.0	39.0	33.0	39.0
14-15	37.856836538175926	39.0	37.0	40.0	34.0	41.0
16-17	37.792599466791685	39.0	37.0	40.0	34.0	41.0
18-19	37.91096349720003	39.0	37.0	40.0	34.0	41.0
20-21	37.9544210718702	39.0	37.0	40.0	34.0	41.0
22-23	37.930087120661724	39.0	37.0	40.0	34.0	41.0
24-25	37.88586489540677	39.0	37.0	40.0	34.0	41.0
26-27	38.136261670788954	39.0	37.0	40.0	34.0	41.0
28-29	38.3188021624827	39.0	38.0	40.0	35.0	41.0
30-31	38.24459278977983	39.0	37.0	40.0	35.0	41.0
32-33	38.195530319633306	39.0	37.0	40.0	35.0	41.0
34-35	38.15670105520442	39.0	37.0	40.0	35.0	41.0
36-37	38.136503977387214	39.0	37.0	40.0	35.0	41.0
38-39	38.06397911052205	39.0	37.0	40.0	34.0	41.0
40-41	37.96309152713633	39.0	37.0	40.0	34.0	41.0
42-43	37.885495481537205	39.0	37.0	40.0	34.0	41.0
44-45	37.784014240295896	39.0	37.0	40.0	34.0	41.0
46-47	37.6691338857014	39.0	37.0	40.0	33.0	41.0
48-49	37.56098366042717	39.0	36.0	40.0	33.0	41.0
50-51	37.43260357703945	39.0	36.0	40.0	33.0	41.0
52-53	37.2751048233597	39.0	36.0	40.0	33.0	41.0
54-55	37.07531400178914	38.0	35.5	40.0	33.0	41.0
56-57	36.82189541827792	38.0	35.0	40.0	32.5	40.5
58-59	36.539208445488775	38.0	35.0	40.0	32.0	40.0
60-61	36.18249928217841	37.5	35.0	39.5	31.5	40.0
62-63	35.876018229591054	37.0	34.5	39.0	31.0	40.0
64-65	35.558931747676816	36.5	34.0	39.0	31.0	40.0
66-67	35.19917321402911	36.0	34.0	38.5	31.0	40.0
68-69	34.83881687481485	35.5	34.0	38.0	30.5	40.0
70-71	34.457049784038055	35.0	33.5	37.5	30.0	39.0
72-73	34.07485186651007	35.0	33.0	37.0	30.0	39.0
74-75	33.68590669362946	35.0	33.0	36.0	29.5	39.0
76-77	33.301941048450345	34.5	33.0	36.0	29.0	38.0
78-79	32.917027316824374	34.0	32.0	35.5	29.0	37.0
80-81	32.49028512435507	34.0	32.0	35.0	29.0	36.5
82-83	32.233079413144395	34.0	32.0	35.0	28.5	36.0
84-85	31.90524059265705	34.0	31.0	35.0	27.0	35.5
86-87	31.64708746398516	34.0	31.0	35.0	27.0	35.0
88-89	31.35808446398596	33.5	31.0	34.0	26.5	35.0
90-91	31.08075205224724	33.0	31.0	34.0	26.0	35.0
92-93	30.78755411090337	33.0	30.0	34.0	25.5	35.0
94-95	30.475194681510658	33.0	30.0	34.0	25.0	35.0
96-97	30.101393068934154	33.0	30.0	34.0	24.5	35.0
98-99	29.596924646260465	32.0	29.0	34.0	23.5	35.0
100-101	27.440445426693756	30.5	26.5	33.0	11.0	34.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.066162751126253
1101	2	1.5297389960281613
1101	3	2.717805038448091
1101	4	1.76226158963388
1101	5	0.5860733925650763
1101	6	0.6613349413534735
1101	7	0.17100913004808405
1101	8	0.4180186575835094
1101	9	0.5901842932388419
1101	10-11	0.5270588075467018
1101	12-13	0.4338926711132487
1101	14-15	0.35455093636216617
1101	16-17	0.22249141119920068
1101	18-19	0.17596771521390053
1101	20-21	0.1526965194311316
1101	22-23	0.17052915719555983
1101	24-25	0.13852793149535358
1101	26-27	0.11709265167964134
1101	28-29	0.1392755487245836
1101	30-31	0.1761198242032478
1101	32-33	0.17032863815498445
1101	34-35	0.056082648689738335
1101	36-37	0.03680767810948993
1101	38-39	0.04929440012075048
1101	40-41	0.05371836218742487
1101	42-43	0.13609746093118247
1101	44-45	0.11571634240274165
1101	46-47	0.14334123329084036
1101	48-49	0.08149042658479289
1101	50-51	0.12735479137542427
1101	52-53	0.06675899931997265
1101	54-55	0.10043126924200863
1101	56-57	0.1443219004285652
1101	58-59	0.16536021420287028
1101	60-61	0.16154652065676345
1101	62-63	0.18502134072349463
1101	64-65	0.1056367374971785
1101	66-67	0.07263367529446185
1101	68-69	0.06524843766955968
1101	70-71	0.08376069379018247
1101	72-73	0.08253477370400475
1101	74-75	0.07601542032004716
1101	76-77	0.0409840938925754
1101	78-79	0.04514671460922415
1101	80-81	0.11284714910523519
1101	82-83	0.109430067074058
1101	84-85	0.1519571255332579
1101	86-87	0.12165353139240764
1101	88-89	0.09432420766837168
1101	90-91	0.09008286378931274
1101	92-93	0.19454065585933122
1101	94-95	0.19319053671409137
1101	96-97	0.19588340028218099
1101	98-99	0.28407084884693745
1101	100-101	0.17743499076454228
1102	1	0.6389734893722583
1102	2	0.7985980939596615
1102	3	-0.9304444968907681
1102	4	1.1541908328931427
1102	5	1.1052309955454334
1102	6	0.8291920983885959
1102	7	0.8650638512835727
1102	8	0.5808444520571143
1102	9	0.6999923995246107
1102	10-11	0.6176488931212489
1102	12-13	0.3633374140774066
1102	14-15	0.22796588516177962
1102	16-17	0.15041290182420397
1102	18-19	0.10902264982302512
1102	20-21	0.10784984289229271
1102	22-23	0.08067889793123584
1102	24-25	0.08302097217258364
1102	26-27	0.039501686816393544
1102	28-29	0.03599912813105277
1102	30-31	0.04169189837123355
1102	32-33	0.041719754306022594
1102	34-35	-0.03213432736870914
1102	36-37	0.006128700345030325
1102	38-39	0.09810878795361333
1102	40-41	0.02471639446045515
1102	42-43	0.05828504587069716
1102	44-45	0.04129963066478126
1102	46-47	0.02316052023019921
1102	48-49	0.022133609165969403
1102	50-51	-0.01927532583966496
1102	52-53	-0.06447908739177421
1102	54-55	-0.025289209020797898
1102	56-57	0.008957514434925429
1102	58-59	-0.07008058423343044
1102	60-61	-0.06987494887727763
1102	62-63	0.007944060530071795
1102	64-65	-0.047945701494356285
1102	66-67	-0.233428410431209
1102	68-69	-0.19698733595929951
1102	70-71	-0.21210920723053306
1102	72-73	-0.18317947328974782
1102	74-75	-0.08889666811703023
1102	76-77	-0.08221514923430107
1102	78-79	-0.11248849375059677
1102	80-81	-0.16642252324136564
1102	82-83	-0.14552758468244065
1102	84-85	-0.10705527073016441
1102	86-87	-0.1682346524893248
1102	88-89	-0.1240915312844777
1102	90-91	-0.05611646786679714
1102	92-93	-0.07040777512866825
1102	94-95	-0.17204547054824104
1102	96-97	-0.13954048040724132
1102	98-99	-0.12985399783195106
1102	100-101	-0.29162789454027305
1103	1	0.2388709009113974
1103	2	0.6736125984064287
1103	3	1.1951569632054664
1103	4	1.9722546437833586
1103	5	1.3747989853429772
1103	6	0.9739795526656962
1103	7	0.8465512972104534
1103	8	0.6129353371916579
1103	9	0.6200156933950041
1103	10-11	0.4591231522015704
1103	12-13	0.27597292898797576
1103	14-15	0.20600317053663275
1103	16-17	0.17992335099032175
1103	18-19	0.06870625226129334
1103	20-21	-0.011240611717738602
1103	22-23	0.05931317006827186
1103	24-25	0.0014897146831458485
1103	26-27	-0.00782347631118796
1103	28-29	0.006741896783239554
1103	30-31	0.0622990572239317
1103	32-33	0.05429497409583206
1103	34-35	0.00876495917517417
1103	36-37	-0.04254334299804441
1103	38-39	-0.019501941975214265
1103	40-41	0.042018708339931266
1103	42-43	-0.09464279193108638
1103	44-45	-0.022050912804637335
1103	46-47	-0.020994269056792803
1103	48-49	-0.003612190508917479
1103	50-51	-0.037242946492256124
1103	52-53	-0.008093389481174995
1103	54-55	-0.043677594185155044
1103	56-57	-0.019080258940007866
1103	58-59	-0.018673029341556457
1103	60-61	-0.08940254311280427
1103	62-63	-0.024692842469605125
1103	64-65	-0.15081286461976617
1103	66-67	-0.2506575242989797
1103	68-69	-0.23168197262155132
1103	70-71	-0.2204729863465147
1103	72-73	-0.14970104126653183
1103	74-75	-0.19808072114203412
1103	76-77	-0.18695026821413308
1103	78-79	-0.144113067699422
1103	80-81	-0.321999659244824
1103	82-83	-0.3514554842975741
1103	84-85	-0.29578767426068
1103	86-87	-0.317495983037805
1103	88-89	-0.3307508887944195
1103	90-91	-0.2777682722199728
1103	92-93	-0.2793271152224328
1103	94-95	-0.3300761624998181
1103	96-97	-0.36182017819421475
1103	98-99	-0.3275889183584262
1103	100-101	-0.501943999301961
1104	1	-0.11115293037944696
1104	2	-0.34414354727411123
1104	3	0.8927927149502963
1104	4	-0.018618194044400127
1104	5	0.06246356007157061
1104	6	0.1299755252551904
1104	7	0.21963148070898342
1104	8	0.188351881435473
1104	9	0.11962712635303774
1104	10-11	0.12897059070861872
1104	12-13	0.03483515178422891
1104	14-15	0.03130996928985752
1104	16-17	0.0015724174146924952
1104	18-19	0.05142930267508916
1104	20-21	-0.02644878250110594
1104	22-23	-0.05294732358455434
1104	24-25	-0.027417981139734593
1104	26-27	0.022656439460817523
1104	28-29	7.047435303917382E-4
1104	30-31	-0.01518866207093339
1104	32-33	-0.002245774145116286
1104	34-35	-0.029857528492442498
1104	36-37	0.017679367791068046
1104	38-39	0.017166693095568064
1104	40-41	0.005241238085133659
1104	42-43	0.01730099535232199
1104	44-45	4.710760524559987E-4
1104	46-47	7.327678037185592E-4
1104	48-49	0.0750088872544552
1104	50-51	0.05343134008306549
1104	52-53	0.0503208098664345
1104	54-55	0.026564272131821554
1104	56-57	0.12600216851780033
1104	58-59	0.12523210396829398
1104	60-61	-0.019391064682281467
1104	62-63	0.10771361937914747
1104	64-65	0.12402107537934626
1104	66-67	0.02896806874567659
1104	68-69	-0.021843681624694966
1104	70-71	0.14017058862662424
1104	72-73	0.03709586403379461
1104	74-75	0.10467501249090105
1104	76-77	0.07759457019493254
1104	78-79	0.08319815653602092
1104	80-81	-0.004666048428617842
1104	82-83	0.08897150946259558
1104	84-85	0.06361426930812542
1104	86-87	0.08024698943554043
1104	88-89	0.011444145247565984
1104	90-91	0.020133318497940422
1104	92-93	0.11524520587783016
1104	94-95	0.07516263581624116
1104	96-97	-0.0661869509972739
1104	98-99	-0.1169050328679262
1104	100-101	-0.13181616762600257
1105	1	-0.919128743128141
1105	2	-0.5566613579963722
1105	3	-1.5700239270281635
1105	4	-0.3805936032138071
1105	5	-0.17446273549026614
1105	6	-0.45615261045747957
1105	7	-0.45120076285752475
1105	8	-0.41355129605191365
1105	9	-0.4958395874848236
1105	10-11	-0.29268463923553867
1105	12-13	-0.3729053661772994
1105	14-15	-0.2973987809113936
1105	16-17	-0.3815336397603062
1105	18-19	-0.1566870158945619
1105	20-21	-0.20543317356231938
1105	22-23	-0.1359066386664054
1105	24-25	-0.008796069250429639
1105	26-27	-0.06552954751698792
1105	28-29	0.015079884468960358
1105	30-31	-0.015828413492648963
1105	32-33	-0.02629134588205062
1105	34-35	0.09056168652924157
1105	36-37	0.09636736993843442
1105	38-39	0.10740335843989612
1105	40-41	0.14565730888745065
1105	42-43	0.12724078331432764
1105	44-45	0.11379765777145678
1105	46-47	0.18606209158551934
1105	48-49	0.21823175478725432
1105	50-51	0.18000721405630316
1105	52-53	0.14860884644370742
1105	54-55	0.0665797987897534
1105	56-57	0.04538839128834837
1105	58-59	0.03666186303239982
1105	60-61	0.02487431549438668
1105	62-63	0.07756972036217746
1105	64-65	0.06826892048647437
1105	66-67	0.08116216951672328
1105	68-69	0.1473411664627946
1105	70-71	0.03378683926121795
1105	72-73	0.1331725137471267
1105	74-75	0.10327878702429416
1105	76-77	0.19704568102202558
1105	78-79	0.20583533851874591
1105	80-81	0.03039164183162768
1105	82-83	0.09712211567330087
1105	84-85	0.11545085147054124
1105	86-87	0.15536004261718972
1105	88-89	0.06202161693951069
1105	90-91	0.11836486108847666
1105	92-93	0.18039670342493608
1105	94-95	0.21877029082850896
1105	96-97	0.0939852981605327
1105	98-99	0.14924424451885443
1105	100-101	0.03786503319073731
1106	1	0.28693599099329603
1106	2	0.4534732732250859
1106	3	1.1912346735688892
1106	4	1.0055916477085987
1106	5	0.8667248302099964
1106	6	0.6477647096441288
1106	7	0.5649192340657834
1106	8	0.39457788774176805
1106	9	0.4554645330455216
1106	10-11	0.29914302583488706
1106	12-13	0.11613487419193547
1106	14-15	-0.010413371510161085
1106	16-17	-0.10906521741001285
1106	18-19	-0.1517552329060763
1106	20-21	-0.10561488886511938
1106	22-23	-0.1261110405660233
1106	24-25	-0.13844938954166963
1106	26-27	-0.0831117406750792
1106	28-29	-0.07187521754816828
1106	30-31	-0.0554834366089878
1106	32-33	-0.023649225697106147
1106	34-35	-0.03682051498172001
1106	36-37	-0.03731750163775871
1106	38-39	-0.03465075334251111
1106	40-41	-0.10835356178023403
1106	42-43	-0.12132963374398287
1106	44-45	-0.07014607224433433
1106	46-47	-0.06665577690316837
1106	48-49	-0.0955234841008803
1106	50-51	-0.03933379102008416
1106	52-53	-0.1359612442397875
1106	54-55	-0.07510597225218874
1106	56-57	-0.06138325712535675
1106	58-59	-0.18641080422219147
1106	60-61	-0.16312269456405204
1106	62-63	-0.23890866802997834
1106	64-65	-0.3875247483904758
1106	66-67	-0.32834150534430506
1106	68-69	-0.2897682702036448
1106	70-71	-0.27502871527199346
1106	72-73	-0.28107480289239817
1106	74-75	-0.2726842726363472
1106	76-77	-0.47776749600615176
1106	78-79	-0.35152559864136634
1106	80-81	-0.4271987413346565
1106	82-83	-0.44354662050635696
1106	84-85	-0.37659215002314284
1106	86-87	-0.5377262536882448
1106	88-89	-0.366629286017357
1106	90-91	-0.37281144919592535
1106	92-93	-0.449983502265372
1106	94-95	-0.3975853119417394
1106	96-97	-0.3334157779410738
1106	98-99	-0.3388950788242511
1106	100-101	-0.664408657564941
1107	1	-2.133290867021582
1107	2	-0.42469339065586453
1107	3	-1.7000226140017602
1107	4	-3.610718330352533
1107	5	-1.963443838668347
1107	6	-0.4530901999665389
1107	7	-0.26784284754422316
1107	8	0.03657145830676711
1107	9	0.274841064403617
1107	10-11	0.3621983607796224
1107	12-13	0.23416480789007466
1107	14-15	0.04905652090904056
1107	16-17	0.0018951504628006433
1107	18-19	-0.04152063419221008
1107	20-21	-0.05067574060799984
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2216	8	0.709649196581708
2216	9	0.6932262473718538
2216	10-11	0.5192950761142754
2216	12-13	0.3871098157846049
2216	14-15	0.39266095475571205
2216	16-17	0.24475785518846038
2216	18-19	0.11069902697435907
2216	20-21	0.09276899537624672
2216	22-23	0.03811093861126125
2216	24-25	0.12770252257339365
2216	26-27	0.05038702002497786
2216	28-29	0.05239179225121404
2216	30-31	0.05254950581843332
2216	32-33	0.05479824593649596
2216	34-35	0.01637371025260137
2216	36-37	-0.0761930622553848
2216	38-39	-0.04087661665317199
2216	40-41	-0.0270576714217583
2216	42-43	-0.06076149806070674
2216	44-45	-0.04494142004158164
2216	46-47	0.07520316412944084
2216	48-49	0.07802676869619063
2216	50-51	0.024914319556941678
2216	52-53	-0.030094423255114577
2216	54-55	-0.011884275394621113
2216	56-57	-0.04912596276294323
2216	58-59	-0.009511656854336081
2216	60-61	0.032951744940355354
2216	62-63	-0.06325961931129598
2216	64-65	-0.07937973975779755
2216	66-67	-0.02031493119743999
2216	68-69	-0.03705185831627489
2216	70-71	-0.02177578869206087
2216	72-73	0.01323513473914062
2216	74-75	-0.009653557627849807
2216	76-77	-0.03155814658629197
2216	78-79	-0.10614413678739965
2216	80-81	-0.06806458932594239
2216	82-83	-0.02937273627016168
2216	84-85	-0.012332234828519972
2216	86-87	0.04429379744587081
2216	88-89	0.10503436181805981
2216	90-91	0.13184795393273063
2216	92-93	0.11018301014985354
2216	94-95	-0.022985169324797994
2216	96-97	0.03124864510860803
2216	98-99	0.014873759840281053
2216	100-101	0.1966401575613581
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	7.0
12	27.0
13	134.0
14	412.0
15	1081.0
16	1990.0
17	3572.0
18	5973.0
19	10572.0
20	11328.0
21	11563.0
22	11796.0
23	12199.0
24	14064.0
25	16056.0
26	19578.0
27	24823.0
28	32219.0
29	42909.0
30	58914.0
31	85029.0
32	129197.0
33	208913.0
34	370899.0
35	719652.0
36	1150814.0
37	735585.0
38	57628.0
39	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.323868460063682	42.13970730171462	11.43851491348713	22.097909324734566
2	23.117527569670433	27.52447359329856	33.21641204531886	16.141586791712147
3	22.4528029204113	28.547288074808776	24.094276400657307	24.90563260412262
4	22.69735829789147	27.744816452053065	25.89283922297558	23.664986027079877
5	22.68023084833579	28.781891565932987	25.95446239081145	22.583415194919773
6	22.74974578538935	27.24150388011774	27.41273748996521	22.596012844527696
7	23.492206718703045	28.905103316804038	25.239409890444236	22.36328007404868
8	23.14417363013818	26.345430466836913	27.888298665159578	22.622097237865336
9	22.270050235469565	27.105210836175853	27.71382721064297	22.91091171771161
10-11	23.853472343197126	27.041402985977257	26.371761741567635	22.73336292925798
12-13	23.3856640659181	27.384070809721482	26.23397745946549	22.996287664894925
14-15	22.98812604010542	27.234151128137242	26.59137634696913	23.186346484788203
16-17	23.21849793457374	27.597008938450912	26.38769697947632	22.796796147499023
18-19	22.6805799623816	27.738365603862565	27.009612788436506	22.571441645319336
20-21	22.198937062098725	27.922537350137183	27.490493045091508	22.388032542672587
22-23	21.831958300251994	27.63498056329126	28.25839490008164	22.274666236375104
24-25	21.744147245344596	27.324985998130586	28.948038815081933	21.982827941442885
26-27	21.705948165387166	27.051638466246064	29.893703533930015	21.348709834436757
28-29	21.789705167325348	27.19686186864815	29.711284530028227	21.302148433998276
30-31	21.74903084050553	27.316503260234608	29.585381432865553	21.34908446639431
32-33	21.77314108291649	27.146567528346928	29.78445817916559	21.29583320957099
34-35	21.851787034576656	27.35054124952067	29.501330077246436	21.296341638656237
36-37	21.866063188101904	27.52807665826153	29.145550134987925	21.46031001864864
38-39	21.942434588591226	27.36216821991752	29.31825546741893	21.377141724072324
40-41	22.112263282776752	27.63239827872671	28.713920601038108	21.541417837458432
42-43	22.14472246595599	27.831341227899053	28.37110560045341	21.652830705691542
44-45	22.100462376113896	27.76798828793463	28.510977117747625	21.620572218203847
46-47	22.156657169746545	28.095296201419266	28.1720154744406	21.57603115439359
48-49	22.20791484936718	28.23563600866042	27.875895671422086	21.680553470550315
50-51	22.116277196607655	27.95808724696622	28.26017440188001	21.66546115454612
52-53	22.169060163484033	28.206334437694824	27.994761039667903	21.629844359153235
54-55	22.2045966806003	28.275949083235464	27.798534172187807	21.720920063976436
56-57	22.240414171684726	28.030083481379855	28.067198804602945	21.662303542332477
58-59	22.3775696072867	28.255437983559546	27.709920334514226	21.65707207463953
60-61	22.280018121482975	28.29604541181552	27.569446730145376	21.854489736556133
62-63	22.310497107172303	28.1351276063346	27.826363974748737	21.728011311744364
64-65	22.358436618026058	28.43148824418297	27.504046694127187	21.706028443663783
66-67	22.367240469028506	28.32463785800432	27.414027986612794	21.89409368635438
68-69	22.324973956389094	28.342620191966766	27.44623295524941	21.88617289639473
70-71	22.409359804827453	28.4390210224723	27.29112194511053	21.86049722758972
72-73	22.335677726604835	28.542606758735147	27.100701872972472	22.021013641687546
74-75	22.371936748210665	28.39579117051347	27.274691657829365	21.9575804234465
76-77	22.47568304102675	28.51093697860932	27.03464623102857	21.978733749335362
78-79	22.418484768936374	28.559505335963248	26.945885216514526	22.076124678585852
80-81	22.376713305669437	28.42063729712676	27.12293895559568	22.079710441608125
82-83	22.40711201308215	28.614616372861555	26.88670674693424	22.09156486712206
84-85	22.453847349786635	28.573781489488496	26.847022518859376	22.125348641865497
86-87	22.393411187206	28.439087921036148	27.074450876518362	22.093050015239495
88-89	22.463346945853104	28.604166817188435	26.76116490201635	22.17132133494211
90-91	22.45240234080751	28.710227800313675	26.615352792252395	22.22201706662642
92-93	22.399873588473753	28.50955886819404	26.860148017086427	22.23041952624578
94-95	22.513788462994793	28.69604530477782	26.59437340262954	22.195792829597853
96-97	22.434687601100457	28.759264447614548	26.52657839802558	22.279469553259418
98-99	22.42071918096891	28.514710057610365	26.793918438876524	22.2706523225442
100-101	22.39545828325976	28.66690429036546	26.470704717499405	22.46693270887538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19865.0
1	12842.0
2	4098.0
3	1937.0
4	1346.5
5	1095.5
6	953.0
7	882.5
8	836.0
9	791.5
10	775.5
11	779.5
12	800.5
13	1082.0
14	1350.5
15	1667.5
16	5965.0
17	6383.5
18	2621.0
19	2609.0
20	3174.5
21	4063.0
22	5187.0
23	6748.5
24	8644.5
25	10992.0
26	14206.5
27	18759.0
28	24394.5
29	32008.0
30	41828.0
31	51100.5
32	60707.0
33	75319.5
34	91434.0
35	106283.5
36	117994.0
37	125173.5
38	130042.5
39	134961.5
40	136849.0
41	133339.0
42	129566.0
43	130578.0
44	133627.0
45	135609.0
46	137060.0
47	137812.0
48	138316.0
49	134914.0
50	129108.5
51	124589.0
52	122776.0
53	119827.5
54	111530.5
55	102097.5
56	93027.0
57	82217.5
58	72583.0
59	63965.0
60	54367.0
61	45429.0
62	36786.0
63	28059.0
64	20842.5
65	15119.5
66	10705.0
67	7629.5
68	5562.0
69	4314.0
70	3637.0
71	3113.5
72	2733.5
73	2192.5
74	1649.5
75	1305.5
76	966.5
77	655.5
78	419.5
79	202.5
80	60.5
81	35.5
82	33.5
83	14.5
84	1.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050628833120462104
2	3.746319575509881E-4
3	0.014476849216791755
4	0.003478725320116318
5	5.351885107871258E-5
6	2.675942553935629E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3737001.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.48430136020518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.93048043235231	49.67099812272702
2	8.309023601179087	9.718948806008301
3	2.4789893098594646	4.349458735996439
4	1.1470961512735607	2.6834846800085774
5	0.6777774032058226	1.981966895211331
6	0.446543459468879	1.5669469352403895
7	0.30370976583555626	1.2433577429814813
8	0.2100022336077957	0.9825467133307626
9	0.15517867964134366	0.8167964998340771
>10	1.1165333412244918	13.740840481092976
>50	0.17372439048483787	6.9673829300782035
>100	0.05010822775493819	4.241793347693107
>500	4.165020565258057E-4	0.18588495179643535
>1k	2.3139003074539223E-4	0.2736308763409826
>5k	4.627800614907845E-5	0.21673947158640838
>10k+	1.3883401844723534E-4	1.359222810073605
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20970	0.5611451535603014	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	17356	0.4644365896610678	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11894	0.3182766073651037	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8008	0.21428947971916518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3379712769678146E-4	0.0	0.0	0.0	0.0
2	2.408348298542066E-4	0.0	0.0	0.0	0.0
3	3.746319575509881E-4	0.0	0.0	0.0	0.0
4	6.154667874051947E-4	0.0	0.0	0.0	0.0
5	7.225044895626198E-4	0.0	0.0	0.0	0.0
6	8.295421917200451E-4	0.0	0.0	0.0	2.675942553935629E-5
7	0.0010168581704955392	0.0	0.0	0.0	2.675942553935629E-5
8	0.001177414723731677	0.0	0.0	0.0	2.675942553935629E-5
9	0.002649183128396273	0.0	0.0	0.0	2.675942553935629E-5
10-11	0.005820175054809993	0.0	0.0	0.0	2.675942553935629E-5
12-13	0.012322715460873573	0.0	0.0	0.0	2.675942553935629E-5
14-15	0.015052176865887914	0.0	0.0	0.0	2.675942553935629E-5
16-17	0.0193470646649546	0.0	0.0	0.0	5.351885107871258E-5
18-19	0.027214335773525347	0.0	0.0	0.0	5.351885107871258E-5
20-21	0.03326196594541987	0.0	0.0	0.0	6.689856384839073E-5
22-23	0.05325125682331902	0.0	0.0	0.0	1.0703770215742517E-4
24-25	0.07728122095766096	0.0	0.0	0.0	1.0703770215742517E-4
26-27	0.09237353696185792	0.0	0.0	0.0	1.0703770215742517E-4
28-29	0.10733205583835809	1.3379712769678146E-5	0.0	0.0	1.0703770215742517E-4
30-31	0.11612252712803663	2.675942553935629E-5	0.0	1.3379712769678146E-5	1.0703770215742517E-4
32-33	0.12515383324756937	2.675942553935629E-5	0.0	2.675942553935629E-5	1.0703770215742517E-4
34-35	0.13589774260162094	2.675942553935629E-5	0.0	2.675942553935629E-5	1.3379712769678146E-4
36-37	0.1514583485527566	4.0139138309034435E-5	0.0	2.675942553935629E-5	1.6055655323613774E-4
38-39	0.17060471752616602	5.351885107871258E-5	0.0	2.675942553935629E-5	1.6055655323613774E-4
40-41	0.19206577680872977	5.351885107871258E-5	0.0	2.675942553935629E-5	1.739362660058159E-4
42-43	0.22008289534843584	5.351885107871258E-5	0.0	5.351885107871258E-5	1.8731597877549405E-4
44-45	0.2531843047406196	5.351885107871258E-5	0.0	5.351885107871258E-5	2.408348298542066E-4
46-47	0.2933368227624237	5.351885107871258E-5	0.0	5.351885107871258E-5	2.809739681632411E-4
48-49	0.3425607860420696	5.351885107871258E-5	0.0	5.351885107871258E-5	2.9435368093291923E-4
50-51	0.39736408954667124	5.351885107871258E-5	0.0	5.351885107871258E-5	2.9435368093291923E-4
52-53	0.4616669891177444	5.351885107871258E-5	0.0	6.689856384839073E-5	3.211131064722755E-4
54-55	0.534680081701878	5.351885107871258E-5	0.0	8.027827661806887E-5	3.211131064722755E-4
56-57	0.6161090136181393	5.351885107871258E-5	0.0	8.027827661806887E-5	3.478725320116318E-4
58-59	0.7070910604519507	5.351885107871258E-5	0.0	8.027827661806887E-5	3.746319575509881E-4
60-61	0.810877492406344	5.351885107871258E-5	0.0	8.027827661806887E-5	3.880116703206662E-4
62-63	0.9243508364059845	5.351885107871258E-5	0.0	8.027827661806887E-5	4.0139138309034435E-4
64-65	1.0530502935375186	5.351885107871258E-5	0.0	8.027827661806887E-5	4.0139138309034435E-4
66-67	1.1928415325551156	5.351885107871258E-5	0.0	1.0703770215742517E-4	4.0139138309034435E-4
68-69	1.3446209942143446	5.351885107871258E-5	0.0	1.0703770215742517E-4	4.0139138309034435E-4
70-71	1.5077330725894909	5.351885107871258E-5	0.0	1.3379712769678146E-4	4.0139138309034435E-4
72-73	1.6884528529695335	5.351885107871258E-5	0.0	1.3379712769678146E-4	4.0139138309034435E-4
74-75	1.8797559861503919	8.027827661806887E-5	0.0	1.3379712769678146E-4	4.147710958600225E-4
76-77	2.0863521310269917	8.027827661806887E-5	0.0	1.3379712769678146E-4	4.5491023416905697E-4
78-79	2.3130847436219577	8.027827661806887E-5	0.0	1.3379712769678146E-4	4.5491023416905697E-4
80-81	2.5488754217619958	8.027827661806887E-5	0.0	1.6055655323613774E-4	4.682899469387351E-4
82-83	2.8014710191407497	8.027827661806887E-5	0.0	1.8731597877549405E-4	4.816696597084132E-4
84-85	3.067259013310406	8.027827661806887E-5	0.0	1.8731597877549405E-4	4.816696597084132E-4
86-87	3.3511229994318974	8.027827661806887E-5	0.0	1.8731597877549405E-4	4.816696597084132E-4
88-89	3.6475505358441165	8.027827661806887E-5	0.0	1.8731597877549405E-4	4.816696597084132E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	4115	0.0	73.46072	1
GTCGACG	3305	0.0	73.20053	1
TATCTCG	4330	0.0	71.755356	2
TCGACGG	4105	0.0	60.527534	2
CGACGGC	4720	0.0	53.345562	3
ATCTCGA	6755	0.0	45.78467	3
TCTCGAG	6740	0.0	45.31661	4
ACGGCGC	6125	0.0	43.35055	5
GACGGCG	6065	0.0	43.23292	4
CTCGAGG	7465	0.0	42.186413	5
TCGAGGG	7875	0.0	39.748775	6
CGAGGGC	7975	0.0	39.25036	7
CGGCGCG	7005	0.0	38.44712	6
AGGGCGC	8575	0.0	36.725548	9
GAGGGCG	8595	0.0	36.4743	8
CTCGACG	1170	0.0	33.290237	5
TTTCTCG	1090	0.0	31.381266	2
GTCGCGC	220	0.0	30.232203	2
GGGCGCG	8685	0.0	27.23632	8
TTCTCGA	1280	0.0	25.609646	3
>>END_MODULE