FastQCFastQC Report
Tue 31 May 2016
SRR2048250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031247
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT119270.29586378606917413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG140550.089.063791
TATCTCG77450.084.8580862
ATATCTC79150.083.431981
ATCTCGA82550.079.729643
TCGACGG157900.079.333342
CGACGGC163200.076.697773
TCTCGAG65300.076.266744
TCGAGGG67450.073.7432866
CTCGAGG67450.073.6874855
CGGCGCG170200.073.521446
ACGGCGC172400.072.62525
AGGGCGC68600.072.485489
GACGGCG172900.072.449864
CGAGGGC69400.071.596577
CTCGACG22300.070.315825
GAGGGCG77600.064.0826348
TCTCGAC26300.060.1693884
CGCGCCG232900.054.0590869
GCGCGCC237550.053.0041738
GGCGCGC237900.052.8741727
Produced by FastQC (version 0.11.5)