##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048250_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4031247 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29384133495169 31.0 31.0 34.0 28.0 34.0 2 31.43978401720361 31.0 31.0 34.0 28.0 34.0 3 31.5197024642747 31.0 31.0 34.0 28.0 34.0 4 35.202667313612885 37.0 35.0 37.0 32.0 37.0 5 35.157596644413005 37.0 35.0 37.0 32.0 37.0 6 35.20869621732432 37.0 35.0 37.0 32.0 37.0 7 35.20183580911812 37.0 35.0 37.0 32.0 37.0 8 35.227022556543915 37.0 35.0 37.0 32.0 37.0 9 36.81926336937429 39.0 37.0 39.0 32.0 39.0 10-11 36.71791867379994 39.0 37.0 39.0 32.0 39.0 12-13 36.57343509340906 39.0 37.0 39.0 32.0 39.0 14-15 37.852267797036504 40.0 38.0 41.0 32.0 41.0 16-17 37.93004782391156 40.0 38.0 41.0 32.5 41.0 18-19 37.86529019432449 40.0 37.5 41.0 32.0 41.0 20-21 37.859577321855994 40.0 38.0 41.0 32.0 41.0 22-23 37.98942399212949 40.0 38.0 41.0 32.0 41.0 24-25 37.84828367004056 40.0 37.5 41.0 32.0 41.0 26-27 37.91072092580782 40.0 38.0 41.0 32.0 41.0 28-29 37.79244362848518 40.0 37.5 41.0 32.0 41.0 30-31 37.58069078873113 40.0 36.5 41.0 32.0 41.0 32-33 37.37176325340521 39.5 36.0 41.0 31.5 41.0 34-35 37.30312853566154 40.0 35.5 41.0 31.5 41.0 36-37 37.03370135841341 39.0 35.0 40.5 30.5 41.0 38-39 36.90277747803595 39.0 35.0 40.5 30.0 41.0 40-41 36.67874704774974 39.0 35.0 41.0 29.5 41.0 42-43 36.64179123730201 39.0 35.0 41.0 29.5 41.0 44-45 36.42835045830732 39.0 35.0 40.5 29.0 41.0 46-47 36.31452910228522 39.0 35.0 40.0 29.0 41.0 48-49 36.17436279642503 39.0 35.0 40.0 28.5 41.0 50-51 36.11184380416283 38.5 34.0 40.0 28.5 41.0 52-53 35.92312105906683 38.0 34.0 40.0 28.0 41.0 54-55 35.84017836168312 38.0 34.0 40.0 28.0 41.0 56-57 35.734025848577375 38.0 34.0 40.0 28.0 41.0 58-59 35.59100843982023 38.0 34.0 40.0 28.0 41.0 60-61 35.337743879251256 37.5 34.0 40.0 28.0 41.0 62-63 35.01585948467063 37.0 34.0 40.0 28.0 41.0 64-65 34.66282319093819 36.5 33.0 39.5 27.0 41.0 66-67 34.3179078334818 36.0 33.0 39.0 26.5 41.0 68-69 33.964654485324274 35.5 33.0 39.0 26.0 40.5 70-71 33.586744746724776 35.0 33.0 38.5 26.0 40.0 72-73 33.30998026169074 35.0 32.5 37.0 26.0 40.0 74-75 33.28246272183272 35.0 33.0 37.0 26.5 39.0 76-77 33.023138497839504 35.0 33.0 36.5 26.5 39.0 78-79 32.73668643970464 35.0 33.0 36.0 26.0 38.5 80-81 32.47096754428593 35.0 33.0 36.0 26.0 37.0 82-83 32.21858558902494 35.0 32.5 35.0 26.0 37.0 84-85 32.01541706573673 35.0 32.0 35.0 26.0 36.5 86-87 31.83170046390112 35.0 32.0 35.0 25.0 36.0 88-89 31.665430200630226 35.0 32.0 35.0 25.0 36.0 90-91 31.50043882203199 35.0 32.0 35.0 25.0 36.0 92-93 31.359114437790588 35.0 32.0 35.0 25.0 35.0 94-95 31.225206989301327 35.0 32.0 35.0 24.5 35.0 96-97 31.067397755582824 35.0 32.0 35.0 24.0 35.0 98-99 30.892386400535614 35.0 32.0 35.0 23.5 35.0 100-101 30.30918609055709 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5075875938021781 1101 2 0.5300892886613262 1101 3 0.588910511102462 1101 4 0.47692146733611196 1101 5 0.5059758856307894 1101 6 0.5634089951842967 1101 7 0.5269319815130871 1101 8 0.5283598082903609 1101 9 0.6581555528196787 1101 10-11 0.6511802831872657 1101 12-13 0.6713357810333207 1101 14-15 0.8198874500024331 1101 16-17 0.6930624483207595 1101 18-19 0.7124173893633596 1101 20-21 0.7278690004015047 1101 22-23 0.7187706847295274 1101 24-25 0.7402558870760174 1101 26-27 0.7188641021274478 1101 28-29 0.7946630934756911 1101 30-31 0.7431323394831395 1101 32-33 0.7812488781785234 1101 34-35 0.8591965035172038 1101 36-37 0.8701048924919519 1101 38-39 0.7997150073135018 1101 40-41 0.6310180118900703 1101 42-43 0.6774346178982498 1101 44-45 0.6323967576295573 1101 46-47 0.6016163235220944 1101 48-49 0.6109930759834228 1101 50-51 0.5321928310895601 1101 52-53 0.5529716347151492 1101 54-55 0.4001382887388232 1101 56-57 0.39688125014966147 1101 58-59 0.3731284748883681 1101 60-61 0.4355579854742331 1101 62-63 0.33586451524693217 1101 64-65 0.34079458730779777 1101 66-67 0.4500953494414617 1101 68-69 0.4118685720076769 1101 70-71 0.35610773146978403 1101 72-73 0.3653644951760171 1101 74-75 0.35983036682239344 1101 76-77 0.3363574363319515 1101 78-79 0.38507744963998647 1101 80-81 0.36413846929528404 1101 82-83 0.37789842480690794 1101 84-85 0.33147972972633966 1101 86-87 0.3572407626250147 1101 88-89 0.39364652011330037 1101 90-91 0.3447192392808702 1101 92-93 0.35829168628884744 1101 94-95 0.3268217522957322 1101 96-97 0.37975268654676597 1101 98-99 0.37411550649358105 1101 100-101 0.4924820381633168 1102 1 0.42150541331860225 1102 2 0.3754748802483725 1102 3 0.39179132289309493 1102 4 0.37251517589039906 1102 5 0.4066430295300876 1102 6 0.4341951441688181 1102 7 0.4493254184118811 1102 8 0.5155011634859008 1102 9 0.5486956324637404 1102 10-11 0.6315932985760924 1102 12-13 0.6291550328025579 1102 14-15 0.7719267230892086 1102 16-17 0.7217599233845036 1102 18-19 0.7321258591327151 1102 20-21 0.7627103120143417 1102 22-23 0.701187692993166 1102 24-25 0.701116024192892 1102 26-27 0.6623487882103944 1102 28-29 0.8257483537745074 1102 30-31 0.7684004413479784 1102 32-33 0.8009622141840183 1102 34-35 0.7749420642506024 1102 36-37 0.813902042158162 1102 38-39 0.806966339445637 1102 40-41 0.7410773330159799 1102 42-43 0.6814029054057258 1102 44-45 0.626613705534794 1102 46-47 0.6149037093416325 1102 48-49 0.6349251399121059 1102 50-51 0.5658810581703335 1102 52-53 0.6031585792865855 1102 54-55 0.5470426582346946 1102 56-57 0.5589058690358684 1102 58-59 0.4530510248985422 1102 60-61 0.4203663646206195 1102 62-63 0.43173453210374646 1102 64-65 0.36569712385472286 1102 66-67 0.43423743841418627 1102 68-69 0.29832886757603205 1102 70-71 0.33163658248740546 1102 72-73 0.3945827001922524 1102 74-75 0.28377698677319074 1102 76-77 0.2956556397090395 1102 78-79 0.3211160687729162 1102 80-81 0.3323287801069057 1102 82-83 0.37205710593739383 1102 84-85 0.39046978083462136 1102 86-87 0.4046048633765942 1102 88-89 0.38470143900295994 1102 90-91 0.40018920884128306 1102 92-93 0.45053575961875936 1102 94-95 0.39380617564461673 1102 96-97 0.48033441519604736 1102 98-99 0.5222520155155408 1102 100-101 0.4838235100631536 1103 1 0.2545011724957398 1103 2 0.28886648863901243 1103 3 0.30328003446142304 1103 4 0.2980478405899376 1103 5 0.3035714655509736 1103 6 0.4162943817630378 1103 7 0.3638056090980726 1103 8 0.39946612700197903 1103 9 0.4775601293375047 1103 10-11 0.4924817635605834 1103 12-13 0.47830047399470743 1103 14-15 0.5613083768787774 1103 16-17 0.5889280391300886 1103 18-19 0.6021185955577479 1103 20-21 0.6777531677703195 1103 22-23 0.6309840477231674 1103 24-25 0.5368185427363841 1103 26-27 0.5275067360570418 1103 28-29 0.6246511278551452 1103 30-31 0.605830150036013 1103 32-33 0.6576890480486739 1103 34-35 0.7168207036247694 1103 36-37 0.7308709522378507 1103 38-39 0.6659254873181624 1103 40-41 0.5880706750200346 1103 42-43 0.6573049882453148 1103 44-45 0.5618254177722406 1103 46-47 0.572370803499922 1103 48-49 0.5018940596695529 1103 50-51 0.5335545687565713 1103 52-53 0.5130069793603838 1103 54-55 0.49241062844334493 1103 56-57 0.46609209657437134 1103 58-59 0.5021547300517639 1103 60-61 0.3862978366106802 1103 62-63 0.3776498350424333 1103 64-65 0.29972884042394554 1103 66-67 0.3717405404824703 1103 68-69 0.39989282982534746 1103 70-71 0.4305190443286122 1103 72-73 0.4589812091864971 1103 74-75 0.3857282392234964 1103 76-77 0.4319460242139854 1103 78-79 0.4345150626783365 1103 80-81 0.44801193317256605 1103 82-83 0.4447402629018171 1103 84-85 0.43814226944921586 1103 86-87 0.3957277428303634 1103 88-89 0.37794478518918595 1103 90-91 0.4372963604943294 1103 92-93 0.4705984787042752 1103 94-95 0.39849512035299384 1103 96-97 0.4541707797840999 1103 98-99 0.4760933638743978 1103 100-101 0.565431277733385 1104 1 0.1863484537119362 1104 2 0.1506086548950485 1104 3 0.20806504663247338 1104 4 0.2376832764286192 1104 5 0.21982496003924723 1104 6 0.23445975155565435 1104 7 0.2626431144858401 1104 8 0.3193310879021638 1104 9 0.4051888032724733 1104 10-11 0.36905404464906155 1104 12-13 0.3685112013510832 1104 14-15 0.520029412320163 1104 16-17 0.4367310119692789 1104 18-19 0.4792318988531079 1104 20-21 0.5334103735617717 1104 22-23 0.436268163030455 1104 24-25 0.42577669922224004 1104 26-27 0.420425895169636 1104 28-29 0.5402815635204021 1104 30-31 0.47977266148578224 1104 32-33 0.473724114332704 1104 34-35 0.5376591872138121 1104 36-37 0.5608053229449439 1104 38-39 0.5919944572302711 1104 40-41 0.5973236847098207 1104 42-43 0.5315127137767703 1104 44-45 0.4599781957106046 1104 46-47 0.4747055397821782 1104 48-49 0.4557132123558034 1104 50-51 0.44173889758492635 1104 52-53 0.4746104416228718 1104 54-55 0.3472396838714289 1104 56-57 0.307015851458317 1104 58-59 0.31270607472897183 1104 60-61 0.36849843186685405 1104 62-63 0.3076490203228417 1104 64-65 0.2759986131709482 1104 66-67 0.3074145031468021 1104 68-69 0.419643568652333 1104 70-71 0.46302714917522536 1104 72-73 0.47379146690946783 1104 74-75 0.463777085292854 1104 76-77 0.4428641809256959 1104 78-79 0.37904813289937067 1104 80-81 0.40797991980149106 1104 82-83 0.41713115030353975 1104 84-85 0.44009688392148405 1104 86-87 0.3706850507171957 1104 88-89 0.43550824660199794 1104 90-91 0.42235104216079833 1104 92-93 0.4914723340299716 1104 94-95 0.4374750833598853 1104 96-97 0.3949113840909284 1104 98-99 0.46490794955991177 1104 100-101 0.4150568831601156 1105 1 0.19687689604059244 1105 2 0.19603783170375877 1105 3 0.2344320857004547 1105 4 0.20686416809181907 1105 5 0.20553839134046825 1105 6 0.26458321576241417 1105 7 0.20572509709022313 1105 8 0.2157122498484867 1105 9 0.3570096923200268 1105 10-11 0.4002249756027254 1105 12-13 0.3422480703039099 1105 14-15 0.36356607558347775 1105 16-17 0.38228205276400473 1105 18-19 0.38015972118926555 1105 20-21 0.42205072023018175 1105 22-23 0.35950533514802174 1105 24-25 0.3566026390661676 1105 26-27 0.3977916102011534 1105 28-29 0.4593902458874837 1105 30-31 0.4395287713651186 1105 32-33 0.3640593260203744 1105 34-35 0.44886312471376044 1105 36-37 0.5425139360400095 1105 38-39 0.4302607190747665 1105 40-41 0.327894865159692 1105 42-43 0.3720304888532482 1105 44-45 0.40420706076866963 1105 46-47 0.4329001851033567 1105 48-49 0.46867323262172533 1105 50-51 0.3238646977949742 1105 52-53 0.1881792900567092 1105 54-55 0.3132156018784613 1105 56-57 0.22606577488961932 1105 58-59 0.21227906566114285 1105 60-61 0.08236534134886142 1105 62-63 0.0833140223523614 1105 64-65 0.035074323548009545 1105 66-67 0.1855622396717891 1105 68-69 0.1426864548338358 1105 70-71 0.14628220814548598 1105 72-73 0.16200057801342638 1105 74-75 0.1888512682831447 1105 76-77 0.17787297017795822 1105 78-79 0.14076634731884496 1105 80-81 0.13697573344329328 1105 82-83 0.21671646187209603 1105 84-85 0.15702079980516714 1105 86-87 0.17271582918205652 1105 88-89 0.1743462152148112 1105 90-91 0.16383397362695717 1105 92-93 0.24273773117046815 1105 94-95 0.2390609366642984 1105 96-97 0.1905312715916878 1105 98-99 0.1155450341172255 1105 100-101 0.05387205793106631 1106 1 0.2133217300955117 1106 2 0.2500821162166993 1106 3 0.1997831973960551 1106 4 0.24429148118380795 1106 5 0.2509366737205667 1106 6 0.23666439384933113 1106 7 0.2634360928703856 1106 8 0.2907133189487183 1106 9 0.37111965267050806 1106 10-11 0.37568801185818756 1106 12-13 0.34958042438177017 1106 14-15 0.48073446585940616 1106 16-17 0.46668385340426966 1106 18-19 0.39181829457626094 1106 20-21 0.45206318924719113 1106 22-23 0.3458205304053692 1106 24-25 0.23365155581468144 1106 26-27 0.35589494323728843 1106 28-29 0.45802814397026737 1106 30-31 0.40921595019254653 1106 32-33 0.40081599595067985 1106 34-35 0.440186201704968 1106 36-37 0.47170088203401406 1106 38-39 0.4607408116303944 1106 40-41 0.44912946647979624 1106 42-43 0.3985817507094893 1106 44-45 0.36996795774491886 1106 46-47 0.3765782601143073 1106 48-49 0.28800658368543 1106 50-51 0.2792013976848011 1106 52-53 0.28565557501928396 1106 54-55 0.2606348840388648 1106 56-57 0.21003972432195894 1106 58-59 0.27009518212115324 1106 60-61 0.2909809606959328 1106 62-63 0.20837735415064174 1106 64-65 0.18842168344200871 1106 66-67 0.20084924011072758 1106 68-69 0.25968880953191587 1106 70-71 0.26744497828831726 1106 72-73 0.17054617097374347 1106 74-75 0.1449447701284683 1106 76-77 0.23091553159225953 1106 78-79 0.23822858787023193 1106 80-81 0.22471048783589254 1106 82-83 0.3088417875550391 1106 84-85 0.2859572609580283 1106 86-87 0.1924017425964486 1106 88-89 0.22862550868335063 1106 90-91 0.1724959382436424 1106 92-93 0.24891839203944244 1106 94-95 0.24807806654637332 1106 96-97 0.18567342433293987 1106 98-99 0.12453591601175873 1106 100-101 0.13322126900976272 1107 1 0.05207890971312423 1107 2 0.01832802759984986 1107 3 0.06670099236181315 1107 4 0.08813902486180325 1107 5 0.032084935163965156 1107 6 0.02633626610958828 1107 7 0.08605020657387996 1107 8 -0.22551308352032606 1107 9 -0.1448103542116428 1107 10-11 -0.030984316152043334 1107 12-13 -0.028592255386250542 1107 14-15 -0.20779551709280497 1107 16-17 -0.1283136097779547 1107 18-19 0.012700657837747542 1107 20-21 -0.0015277594441087672 1107 22-23 -0.01977881038921936 1107 24-25 -0.07528157970739358 1107 26-27 0.03224144316828159 1107 28-29 -0.07752211342952364 1107 30-31 -0.03321880172207159 1107 32-33 -0.010974428220144716 1107 34-35 0.12295304622089276 1107 36-37 0.26099715327909934 1107 38-39 0.13790064624660658 1107 40-41 -0.11550920121248254 1107 42-43 -0.07326685247499398 1107 44-45 -0.10322276433623756 1107 46-47 -0.05360591622216759 1107 48-49 0.0806982840275765 1107 50-51 0.06162035033505475 1107 52-53 0.09805076043087979 1107 54-55 0.07790273333659314 1107 56-57 -0.0955406576613882 1107 58-59 0.056800038418636234 1107 60-61 -0.09077066454539562 1107 62-63 -0.06285721060865512 1107 64-65 -0.19418851487144906 1107 66-67 0.07184121848280256 1107 68-69 0.17072114646130387 1107 70-71 0.14113734919902043 1107 72-73 0.12348700273376068 1107 74-75 0.09269660080409636 1107 76-77 0.107300453551197 1107 78-79 0.13536255245332285 1107 80-81 0.11682254520734148 1107 82-83 0.08079973299172849 1107 84-85 0.05718386947339127 1107 86-87 0.13092554160907355 1107 88-89 0.15257014097788613 1107 90-91 0.13273399722205426 1107 92-93 0.17515164299845054 1107 94-95 0.1480933899661565 1107 96-97 0.1044929222511044 1107 98-99 -0.03395431173164809 1107 100-101 -0.02208053223285944 1108 1 -0.16948750533113355 1108 2 -0.11786031480543357 1108 3 -0.058574586610046 1108 4 -0.19457775983308778 1108 5 0.03398510901118357 1108 6 0.06570022861364322 1108 7 0.059886999100911 1108 8 0.11324577269278535 1108 9 0.19522040494851467 1108 10-11 0.10541201617960638 1108 12-13 0.159442824557253 1108 14-15 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-0.6140161407090048 2215 12-13 -0.4942437591691089 2215 14-15 -0.662816434656115 2215 16-17 -0.7038856193952014 2215 18-19 -0.5981429871141302 2215 20-21 -3.5670806455500994 2215 22-23 -1.383104729043204 2215 24-25 -0.9084287935184605 2215 26-27 -0.8027658203344146 2215 28-29 -0.7256544229625064 2215 30-31 -1.1519035478446469 2215 32-33 -1.0387296577113077 2215 34-35 -1.090103955267928 2215 36-37 -1.171904597486936 2215 38-39 -0.9262839992364675 2215 40-41 -1.2147203300344458 2215 42-43 -1.570361125294923 2215 44-45 -1.0820153733234008 2215 46-47 -0.8644986066239682 2215 48-49 -0.8457136740718738 2215 50-51 -0.822343989970534 2215 52-53 -0.7534459786688004 2215 54-55 -0.7037719179320021 2215 56-57 -0.5132547744630074 2215 58-59 -0.38608047470432894 2215 60-61 -0.4188950593789258 2215 62-63 -0.3720134934130641 2215 64-65 -0.3854965483917283 2215 66-67 -0.4611682857788679 2215 68-69 -0.42643053502101225 2215 70-71 -0.27209798387356443 2215 72-73 -0.3223151161340283 2215 74-75 -0.2425775630602871 2215 76-77 -0.36490531037282636 2215 78-79 -0.3857601779654729 2215 80-81 -0.37235931086593155 2215 82-83 -0.4234870530523125 2215 84-85 -0.5326445828275972 2215 86-87 -0.5081479840222904 2215 88-89 -0.45369468277857194 2215 90-91 -0.4943604431631421 2215 92-93 -0.4552773963227956 2215 94-95 -0.41474872662996987 2215 96-97 -0.3834501800100618 2215 98-99 -0.4314734277175134 2215 100-101 -0.4983883590558662 2216 1 -0.5326949980656934 2216 2 -0.5163973188399922 2216 3 -0.44644408742319186 2216 4 -0.4403489893989132 2216 5 -0.48200337261236115 2216 6 -0.5156698691374046 2216 7 -0.779809405896799 2216 8 -1.545148252286289 2216 9 -0.9653245494466915 2216 10-11 -0.7901987148148493 2216 12-13 -0.8102563382698875 2216 14-15 -1.6228342984249835 2216 16-17 -1.0388400075672735 2216 18-19 -1.035919977851151 2216 20-21 -1.0099042391775512 2216 22-23 -0.8659086227060158 2216 24-25 -1.1015805829806382 2216 26-27 -1.3995914678918027 2216 28-29 -1.964721544181046 2216 30-31 -1.1978484520172117 2216 32-33 -1.0791701180726534 2216 34-35 -2.328760722970948 2216 36-37 -2.1843599000120264 2216 38-39 -2.3882783586363274 2216 40-41 -1.5263989434901575 2216 42-43 -1.4082943424679328 2216 44-45 -1.2170451639086508 2216 46-47 -1.537986092297416 2216 48-49 -1.5497652303903493 2216 50-51 -1.050108299359664 2216 52-53 -0.8706267745156708 2216 54-55 -0.8553131071132682 2216 56-57 -0.8341437459377516 2216 58-59 -0.8170296197643339 2216 60-61 -0.9279224622331768 2216 62-63 -0.8643746169735209 2216 64-65 -0.8389747767391995 2216 66-67 -0.937797113443736 2216 68-69 -0.9631882510659935 2216 70-71 -1.034601196612165 2216 72-73 -0.8390283150620164 2216 74-75 -0.6862991047437887 2216 76-77 -0.804566950711262 2216 78-79 -0.8482419869430338 2216 80-81 -0.9299376387497134 2216 82-83 -1.3053781810423573 2216 84-85 -0.8860582592293085 2216 86-87 -1.031725402216022 2216 88-89 -0.8418933244672431 2216 90-91 -1.0509414147339484 2216 92-93 -1.3401043597560687 2216 94-95 -0.994911235754774 2216 96-97 -0.924381267051011 2216 98-99 -1.0296443423667334 2216 100-101 -1.0148948289158426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 65.0 11 649.0 12 2638.0 13 5290.0 14 7779.0 15 8924.0 16 10165.0 17 12139.0 18 16657.0 19 25486.0 20 24897.0 21 25613.0 22 29571.0 23 33771.0 24 37551.0 25 42196.0 26 49164.0 27 58109.0 28 68859.0 29 80884.0 30 93745.0 31 111262.0 32 136403.0 33 172528.0 34 227037.0 35 325439.0 36 546199.0 37 896991.0 38 830184.0 39 150936.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.79961402356229 39.72178214552349 12.82978131229506 19.648822518619156 2 24.017249962650062 27.263493005759088 31.879622519951816 16.839634511639034 3 22.219453537807848 28.01476227231776 23.58987786627089 26.175906323603503 4 25.375806789491605 25.824511309696963 25.588493427042202 23.211188473769226 5 23.17021176272053 28.777965559087832 24.34186786979062 23.709954808401022 6 22.493154131227804 25.06224362882031 26.494436343778847 25.95016589617304 7 26.04681686737123 25.924371989276455 23.92123829361215 24.107572849740162 8 26.676290991349273 24.636848792001707 25.61510127497079 23.07175894167823 9 22.07907479807101 26.194878842604535 25.666787394074102 26.059258965250354 10-11 25.67999115898314 25.300899072375405 24.681420134225192 24.33768963441626 12-13 24.908111058053073 25.499879069017485 24.551854585144714 25.04015528778473 14-15 24.705552864048634 25.324859923809267 24.91958347876153 25.050003733380567 16-17 24.853776472142275 26.991206805345996 24.670297061017788 23.484719661493944 18-19 23.145828379750956 25.379867673767947 26.413226818443807 25.061077128037283 20-21 23.04125647353479 27.596111959529928 25.159599486781037 24.203032080154244 22-23 22.269409970923608 27.018789683246087 27.49431565779646 23.217484688033846 24-25 22.129223463326767 25.751167360549417 28.828247892300134 23.291361283823683 26-27 22.09721218845142 25.501844284256947 30.084843526100812 22.316100001190822 28-29 22.14358063997906 25.392154491136075 30.27015728109693 22.194107587787936 30-31 22.227930439515227 25.33000200559358 30.18876021696132 22.25330733792987 32-33 22.170870327783856 25.28219974646553 30.289418780901062 22.257511144849556 34-35 22.21572298048776 25.471657042455554 30.220357783257484 22.092262193799204 36-37 22.186256481089394 25.48341652297322 29.992674548782716 22.33765244715467 38-39 22.329453507460332 25.44481301839239 29.909713854468944 22.316019619678325 40-41 22.857973526799036 25.985972810381895 28.295401013750098 22.860652649068967 42-43 22.87357198627941 26.202173439828535 28.140396051516092 22.78385852237596 44-45 23.010310193899716 26.40510563223056 27.770098701216227 22.8144854726535 46-47 22.940519397595832 26.812447860426314 27.564646869814723 22.68238587216313 48-49 22.987539587626358 26.937706868371002 27.303462179320693 22.771291364681943 50-51 22.903586070098022 26.77402037856683 27.51080474848292 22.811588802852224 52-53 22.90494103971191 26.9688975503365 27.364789983393056 22.76137142655853 54-55 22.972604389553968 26.80684377378748 27.333952926698004 22.886598909960547 56-57 22.983731832854698 26.69432064073474 27.551363138998923 22.77058438741164 58-59 23.050423083454096 26.82569512414909 27.405158022311994 22.71872377008482 60-61 22.99589928377001 26.811530030285912 27.35321105355241 22.839359632391666 62-63 23.024562870992522 26.700596614397483 27.50218480782745 22.77265570678254 64-65 23.156365855443415 26.910674484111873 27.232790602197138 22.700169058247575 66-67 23.13669671164007 26.82245351889105 27.109760049930117 22.931089719538768 68-69 23.12128232281475 26.838940903397884 27.09412248864929 22.945654285138072 70-71 23.247113794733405 27.035066344364083 26.894301860127808 22.823518000774705 72-73 23.19250919159828 27.010895464221296 26.708324991993553 23.08827035218687 74-75 23.159144377518185 26.958895484765954 26.921374275980213 22.960585861735648 76-77 23.253748074845277 27.14285519375953 26.715299369078878 22.88809736231632 78-79 23.308650697309997 27.012745329199454 26.672019029373455 23.006584944117094 80-81 23.273661032173578 26.946899213797142 26.799090050224354 22.980349703804922 82-83 23.339038400738836 27.148898042189053 26.613785954917024 22.898277602155083 84-85 23.411205019191332 27.010537930322805 26.615275620670232 22.96298142981564 86-87 23.421561616045857 26.933837098049313 26.701787312958004 22.942813972946833 88-89 23.48380042205303 27.09434574462939 26.512801125805492 22.909052707512092 90-91 23.56330435718774 27.10543412497423 26.34250642543114 22.98875509240689 92-93 23.450857761878645 27.06397052822613 26.50695926099294 22.978212448902287 94-95 23.642721470552413 27.221849715485057 26.297049027261295 22.83837978670124 96-97 23.625096651234717 27.198469853124852 26.26425520440697 22.912178291233456 98-99 23.71836803847544 27.131803136845743 26.361495586849433 22.788333237829388 100-101 23.8795340498858 27.348547484190377 26.023188358341727 22.748730107582098 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2272.0 1 1843.0 2 1142.5 3 774.5 4 612.0 5 463.5 6 349.0 7 285.5 8 233.0 9 201.0 10 201.5 11 182.0 12 182.0 13 235.0 14 260.5 15 295.5 16 2497.0 17 2816.0 18 1043.5 19 1291.5 20 1770.0 21 2434.0 22 3368.0 23 4569.5 24 5990.0 25 7960.0 26 10601.0 27 14560.5 28 19114.5 29 25024.5 30 33297.0 31 41866.5 32 51840.5 33 66360.5 34 81825.5 35 96535.0 36 109085.5 37 117105.5 38 123873.0 39 132144.5 40 136620.5 41 135521.0 42 134417.5 43 136661.5 44 141031.5 45 146291.0 46 149234.0 47 151328.5 48 153494.5 49 151961.0 50 147786.5 51 144188.5 52 145667.5 53 145708.5 54 138511.0 55 129909.0 56 121058.0 57 108274.5 58 96012.0 59 86694.0 60 76299.0 61 64893.5 62 53512.0 63 42512.0 64 32937.5 65 24428.0 66 17678.0 67 12929.0 68 9649.0 69 7360.5 70 5784.5 71 4479.5 72 3640.0 73 2861.5 74 2080.0 75 1605.0 76 1174.5 77 713.5 78 423.5 79 253.5 80 132.5 81 64.5 82 30.0 83 13.5 84 5.5 85 2.0 86 1.5 87 2.5 88 2.0 89 1.5 90 2.5 91 2.0 92 1.0 93 1.5 94 1.0 95 1.5 96 4.0 97 3.0 98 1.5 99 1.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0878140188383396 2 0.04437832759937558 3 0.05102639456227812 4 0.05742639932507236 5 0.0430884041588124 6 0.0177116410877329 7 0.0213829616493358 8 0.012105435365285234 9 0.00315038994137546 10-11 9.054270303953094E-4 12-13 5.457368402382687E-4 14-15 0.001190698560519859 16-17 1.116279900487368E-4 18-19 0.0018232571707960342 20-21 0.0054325621823718565 22-23 0.016310089657120986 24-25 0.01952249514852352 26-27 0.010294581304494614 28-29 0.017972106397846622 30-31 5.333337302328534E-4 32-33 0.005879074142566804 34-35 1.8604665008122796E-4 36-37 0.002381397121039718 38-39 0.009823263124288837 40-41 0.00188527272082311 42-43 0.0019720944908610165 44-45 0.002257366020985566 46-47 0.0 48-49 0.0 50-51 0.003696126781613729 52-53 0.002517831331099285 54-55 0.002691474871175098 56-57 0.0 58-59 0.004626360032019869 60-61 0.0 62-63 0.0 64-65 0.0022821722409963967 66-67 3.1007775013537994E-4 68-69 0.0 70-71 0.001128683010492783 72-73 0.0029767464012996473 74-75 0.004800003572095681 76-77 0.0019224820508393556 78-79 1.3643421005956718E-4 80-81 8.80620810384479E-4 82-83 8.80620810384479E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4031247.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.79918688545189 #Duplication Level Percentage of deduplicated Percentage of total 1 83.81670174580827 48.44537208328155 2 9.026836863265073 10.434876616886887 3 2.684028759067773 4.654040395538573 4 1.179718099817385 2.7274698769398094 5 0.6664152938573875 1.9259131056493244 6 0.4460070674101327 1.546730750488236 7 0.30081782239211113 1.2170917874441416 8 0.21483115446854378 0.9933652836755796 9 0.167155921458934 0.869532870907336 >10 1.2581712839546995 15.431158144086881 >50 0.19469506810636472 7.723078345923158 >100 0.04401626053142556 3.367043060514064 >500 3.887099120342722E-4 0.1702757820722192 >1k 1.727599598771148E-4 0.1963126731747419 >5k 0.0 0.0 >10k+ 4.318998996927865E-5 0.29773922341749043 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 11927 0.29586378606917413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 4.961244002166079E-5 0.0 0.0 0.0 0.0 4 7.441866003249119E-5 0.0 0.0 0.0 0.0 5 1.2403110005415198E-4 0.0 0.0 0.0 2.4806220010830395E-5 6 1.4883732006498238E-4 0.0 0.0 2.4806220010830395E-5 2.4806220010830395E-5 7 2.4806220010830396E-4 0.0 0.0 2.4806220010830395E-5 4.961244002166079E-5 8 3.720933001624559E-4 0.0 0.0 2.4806220010830395E-5 4.961244002166079E-5 9 6.697679402924207E-4 0.0 0.0 2.4806220010830395E-5 4.961244002166079E-5 10-11 9.426363604115552E-4 1.2403110005415197E-5 0.0 2.4806220010830395E-5 4.961244002166079E-5 12-13 0.0012775203305577655 2.4806220010830395E-5 0.0 2.4806220010830395E-5 4.961244002166079E-5 14-15 0.0014387607606281628 2.4806220010830395E-5 0.0 2.4806220010830395E-5 6.201555002707599E-5 16-17 0.0016248074107093908 4.961244002166079E-5 0.0 2.4806220010830395E-5 7.441866003249119E-5 18-19 0.002108528700920584 4.961244002166079E-5 0.0 2.4806220010830395E-5 7.441866003249119E-5 20-21 0.0030263588413213085 4.961244002166079E-5 0.0 2.4806220010830395E-5 7.441866003249119E-5 22-23 0.003906979651705788 4.961244002166079E-5 0.0 2.4806220010830395E-5 1.1162799004873678E-4 24-25 0.004688375582046945 4.961244002166079E-5 0.0 2.4806220010830395E-5 1.2403110005415198E-4 26-27 0.0054697715123881024 4.961244002166079E-5 0.0 3.720933001624559E-5 1.4883732006498238E-4 28-29 0.006648066962902546 4.961244002166079E-5 0.0 4.961244002166079E-5 1.4883732006498238E-4 30-31 0.00787597485343865 4.961244002166079E-5 0.0 4.961244002166079E-5 1.4883732006498238E-4 32-33 0.009413960494110134 4.961244002166079E-5 0.0 4.961244002166079E-5 1.9844976008664316E-4 34-35 0.011113186564852016 4.961244002166079E-5 0.0 4.961244002166079E-5 1.9844976008664316E-4 36-37 0.01369303344597838 6.201555002707599E-5 0.0 4.961244002166079E-5 1.9844976008664316E-4 38-39 0.01670698917729427 7.441866003249119E-5 0.0 4.961244002166079E-5 2.2325598009747356E-4 40-41 0.020936449689140855 9.922488004332158E-5 0.0 4.961244002166079E-5 2.2325598009747356E-4 42-43 0.02653025230158311 9.922488004332158E-5 0.0 4.961244002166079E-5 2.3565909010288876E-4 44-45 0.034443436485038004 9.922488004332158E-5 0.0 4.961244002166079E-5 2.7286842011913436E-4 46-47 0.04411786228926186 9.922488004332158E-5 0.0 4.961244002166079E-5 2.8527153012454954E-4 48-49 0.05595042923442796 9.922488004332158E-5 0.0 6.201555002707599E-5 3.596901901570407E-4 50-51 0.07023881196066627 1.2403110005415198E-4 0.0 7.441866003249119E-5 3.720933001624559E-4 52-53 0.0882977401285508 1.2403110005415198E-4 0.0 7.441866003249119E-5 3.720933001624559E-4 54-55 0.10971791110790284 1.2403110005415198E-4 0.0 7.441866003249119E-5 3.720933001624559E-4 56-57 0.136607853599643 1.4883732006498238E-4 0.0 7.441866003249119E-5 3.968995201732863E-4 58-59 0.16780167526326223 1.6124043007039758E-4 0.0 7.441866003249119E-5 3.968995201732863E-4 60-61 0.20659860336020097 1.7364354007581276E-4 0.0 7.441866003249119E-5 4.093026301787015E-4 62-63 0.24942654220889965 1.7364354007581276E-4 0.0 7.441866003249119E-5 4.3410885018953195E-4 64-65 0.30130875136155144 1.7364354007581276E-4 0.0 9.922488004332158E-5 4.589150702003623E-4 66-67 0.36012429900723025 1.8604665008122796E-4 0.0 1.1162799004873678E-4 4.961244002166079E-4 68-69 0.4267414028463153 1.9844976008664316E-4 0.0 1.2403110005415198E-4 5.209306202274383E-4 70-71 0.5008499851286712 1.9844976008664316E-4 0.0 1.2403110005415198E-4 5.209306202274383E-4 72-73 0.5852159393855052 2.2325598009747356E-4 0.0 1.3643421005956718E-4 5.209306202274383E-4 74-75 0.6837958577085452 2.4806220010830396E-4 0.0 1.4883732006498238E-4 5.209306202274383E-4 76-77 0.7932160941763181 2.4806220010830396E-4 0.0 1.4883732006498238E-4 5.333337302328536E-4 78-79 0.9145185100292788 2.4806220010830396E-4 0.0 1.7364354007581276E-4 5.953492802599295E-4 80-81 1.0495511686582342 2.4806220010830396E-4 0.0 1.7364354007581276E-4 5.953492802599295E-4 82-83 1.1989590317834655 2.7286842011913436E-4 0.0 1.7364354007581276E-4 6.201555002707599E-4 84-85 1.362171556344724 2.8527153012454954E-4 0.0 1.7364354007581276E-4 6.201555002707599E-4 86-87 1.5429716908936615 3.2248086014079517E-4 0.0 1.7364354007581276E-4 6.449617202815903E-4 88-89 1.7426741650908517 3.472870801516255E-4 0.0 1.9844976008664316E-4 7.069772703086662E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 14055 0.0 89.06379 1 TATCTCG 7745 0.0 84.858086 2 ATATCTC 7915 0.0 83.43198 1 ATCTCGA 8255 0.0 79.72964 3 TCGACGG 15790 0.0 79.33334 2 CGACGGC 16320 0.0 76.69777 3 TCTCGAG 6530 0.0 76.26674 4 TCGAGGG 6745 0.0 73.743286 6 CTCGAGG 6745 0.0 73.687485 5 CGGCGCG 17020 0.0 73.52144 6 ACGGCGC 17240 0.0 72.6252 5 AGGGCGC 6860 0.0 72.48548 9 GACGGCG 17290 0.0 72.44986 4 CGAGGGC 6940 0.0 71.59657 7 CTCGACG 2230 0.0 70.31582 5 GAGGGCG 7760 0.0 64.082634 8 TCTCGAC 2630 0.0 60.169388 4 CGCGCCG 23290 0.0 54.059086 9 GCGCGCC 23755 0.0 53.004173 8 GGCGCGC 23790 0.0 52.874172 7 >>END_MODULE