FastQCFastQC Report
Tue 31 May 2016
SRR2048250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031247
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT186510.4626608094219977No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT152550.3784188862652177No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT51530.12782645171580903No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45180.11207450200893172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42550.072.247841
TCGACGG48750.065.106152
ATATCTC39500.061.3469851
CGACGGC54950.057.5872963
TATCTCG43700.055.342212
ACGGCGC64950.048.632055
CGGCGCG65550.048.1869056
GACGGCG65850.047.9828454
ATCTCGA73400.039.8753741
TCTCGAG70050.037.8482862
TCGAGGG77300.034.1755454
CTCGAGG78950.033.521483
GGTCGAC3850.033.3213961
CGAGGGC80450.032.94495
AGGGCGC83950.031.6845427
GGGCGCG85050.031.3305918
GAGGGCG91750.029.094466
CGCGCCG146300.025.8758169
GCGCGCC150000.025.3008778
GGCGCGC151350.025.0438167
Produced by FastQC (version 0.11.5)