FastQCFastQC Report
Tue 31 May 2016
SRR2048249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335923
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT69160.20731893392023737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG142750.089.745841
TATCTCG69800.085.991562
ATATCTC72800.082.536751
ATCTCGA73950.081.100323
TCGACGG161150.079.5940252
CGACGGC165850.077.222643
TCTCGAG56200.076.6947864
TCGAGGG57600.074.801516
CGGCGCG172150.074.33896
ACGGCGC172550.074.183255
GACGGCG173200.073.971914
CGAGGGC58600.073.522827
AGGGCGC58400.073.518489
CTCGAGG58950.073.024345
CTCGACG23100.072.4025655
GAGGGCG66100.065.248558
TCTCGAC25950.064.644594
CGCGCCG225550.056.938349
GGCGCGC229850.056.006367
GCGCGCC230250.055.9263348
Produced by FastQC (version 0.11.5)