##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3335923 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196066875644313 31.0 31.0 34.0 28.0 34.0 2 31.365616052888512 31.0 31.0 34.0 28.0 34.0 3 31.449560736263997 31.0 31.0 34.0 28.0 34.0 4 35.07170878944148 37.0 35.0 37.0 32.0 37.0 5 35.028610672368636 37.0 35.0 37.0 32.0 37.0 6 35.02261443084868 37.0 35.0 37.0 32.0 37.0 7 34.89031671294571 37.0 35.0 37.0 32.0 37.0 8 34.92082581042788 37.0 35.0 37.0 32.0 37.0 9 36.61100211245883 39.0 37.0 39.0 32.0 39.0 10-11 36.624900814557165 39.0 37.0 39.0 32.0 39.0 12-13 36.437470229378796 39.0 37.0 39.0 32.0 39.0 14-15 37.69234346835943 40.0 37.5 41.0 32.0 41.0 16-17 37.78939591831106 40.0 37.5 41.0 32.0 41.0 18-19 37.75303566659063 40.0 37.5 41.0 32.0 41.0 20-21 37.74111692625999 40.0 37.5 41.0 32.0 41.0 22-23 37.88695707304995 40.0 38.0 41.0 32.0 41.0 24-25 37.723728185572625 40.0 37.0 41.0 32.0 41.0 26-27 37.80235305191397 40.0 37.5 41.0 32.0 41.0 28-29 37.70383833799521 40.0 37.0 41.0 32.0 41.0 30-31 37.45612578587695 40.0 36.5 41.0 31.5 41.0 32-33 37.225020481587855 39.5 35.5 40.5 31.0 41.0 34-35 37.17087010101852 39.0 35.0 41.0 31.0 41.0 36-37 36.83378813000181 39.0 35.0 40.5 30.0 41.0 38-39 36.689934689739545 39.0 35.0 40.5 30.0 41.0 40-41 36.39605890183916 39.0 35.0 40.5 29.0 41.0 42-43 36.35288824112547 39.0 35.0 40.5 28.5 41.0 44-45 36.113597046454615 39.0 35.0 40.0 28.0 41.0 46-47 36.00748278662307 38.0 34.0 40.0 28.0 41.0 48-49 35.884747339791716 38.0 34.0 40.0 28.0 41.0 50-51 35.825537639807635 38.0 34.0 40.0 28.0 41.0 52-53 35.645671078139394 38.0 34.0 40.0 28.0 41.0 54-55 35.58203591629663 38.0 34.0 40.0 28.0 41.0 56-57 35.488833375350694 38.0 34.0 40.0 28.0 41.0 58-59 35.36647443601066 37.5 34.0 40.0 28.0 41.0 60-61 35.118374884552196 37.0 33.5 40.0 28.0 41.0 62-63 34.80119415226311 37.0 33.0 40.0 27.0 41.0 64-65 34.4464128818321 36.0 33.0 39.5 26.5 41.0 66-67 34.1033710909994 36.0 33.0 39.0 26.0 41.0 68-69 33.75181906177091 35.0 32.5 39.0 26.0 40.5 70-71 33.37351281789178 35.0 32.0 38.0 26.0 40.0 72-73 33.10210307612016 35.0 32.0 37.0 26.0 39.5 74-75 33.09749160876915 35.0 32.5 37.0 26.0 39.0 76-77 32.8468903508864 35.0 32.5 36.5 26.0 39.0 78-79 32.56741522511161 35.0 32.0 36.0 26.0 38.5 80-81 32.30907053310283 35.0 32.0 36.0 26.0 37.0 82-83 32.05770277071743 35.0 32.0 35.0 25.0 37.0 84-85 31.85334898317497 35.0 32.0 35.0 25.0 36.5 86-87 31.671339086663572 35.0 32.0 35.0 25.0 36.0 88-89 31.5042887680561 35.0 32.0 35.0 25.0 36.0 90-91 31.33843811742657 35.0 32.0 35.0 25.0 36.0 92-93 31.190350466722403 35.0 32.0 35.0 24.0 35.0 94-95 31.047878353307315 35.0 32.0 35.0 24.0 35.0 96-97 30.88055284849201 35.0 32.0 35.0 23.5 35.0 98-99 30.692480312045575 35.0 32.0 35.0 22.5 35.0 100-101 30.083983653099907 34.5 31.0 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48225469726218506 1101 2 0.517431640966155 1101 3 0.532138642024897 1101 4 0.45873068837548203 1101 5 0.4781815577165389 1101 6 0.562116459190662 1101 7 0.5433847665874438 1101 8 0.5974438055396902 1101 9 0.6948380432547694 1101 10-11 0.6556884570725217 1101 12-13 0.6763112275760861 1101 14-15 0.776901047080301 1101 16-17 0.7491969605074047 1101 18-19 0.7642449892356211 1101 20-21 0.8305101208580155 1101 22-23 0.7466838672269489 1101 24-25 0.7582826773481202 1101 26-27 0.774959170845058 1101 28-29 0.8332008661102037 1101 30-31 0.7711541171525198 1101 32-33 0.7500712123227871 1101 34-35 0.8355507764385948 1101 36-37 0.9034710128948333 1101 38-39 0.7945559401911026 1101 40-41 0.7026608223303938 1101 42-43 0.7614052928216424 1101 44-45 0.7265685010609673 1101 46-47 0.675260925009546 1101 48-49 0.6026444916685492 1101 50-51 0.5633533737531877 1101 52-53 0.613634513297626 1101 54-55 0.48395906422040724 1101 56-57 0.5876649594883858 1101 58-59 0.6341154882044009 1101 60-61 0.7212090647244978 1101 62-63 0.6348993678335688 1101 64-65 0.5363039151464264 1101 66-67 0.5190066597591354 1101 68-69 0.5164223246780395 1101 70-71 0.5442577566213913 1101 72-73 0.5948839099729994 1101 74-75 0.6370282626764237 1101 76-77 0.6197620277334295 1101 78-79 0.6668022147234396 1101 80-81 0.6266847954434454 1101 82-83 0.6189765861040328 1101 84-85 0.5784609445263946 1101 86-87 0.5180619815480867 1101 88-89 0.5327085002664376 1101 90-91 0.5591033256937514 1101 92-93 0.5892391760483235 1101 94-95 0.5411438361816039 1101 96-97 0.5064838798636977 1101 98-99 0.611485354929755 1101 100-101 0.6216864708354528 1102 1 0.347561062686772 1102 2 0.3343679070827932 1102 3 0.38920181048208136 1102 4 0.3532374137615264 1102 5 0.3315323847518741 1102 6 0.40891232441217795 1102 7 0.41728620722963683 1102 8 0.43450450523467765 1102 9 0.504490226516559 1102 10-11 0.4852588128131856 1102 12-13 0.48128452910750497 1102 14-15 0.6570393176725631 1102 16-17 0.5803211830464079 1102 18-19 0.5929683405404873 1102 20-21 0.660023257062015 1102 22-23 0.6140064548082762 1102 24-25 0.5600124832715494 1102 26-27 0.6154842009082131 1102 28-29 0.6946416078537823 1102 30-31 0.6521657720992025 1102 32-33 0.6158241051546156 1102 34-35 0.6672841859584651 1102 36-37 0.7483764632846572 1102 38-39 0.7355689489751214 1102 40-41 0.5766254354116498 1102 42-43 0.6766595155995603 1102 44-45 0.6525212242649445 1102 46-47 0.6363114629607907 1102 48-49 0.6311413539514419 1102 50-51 0.6555268202248428 1102 52-53 0.5187610373600933 1102 54-55 0.5196889219082124 1102 56-57 0.3932310227683331 1102 58-59 0.33459114302365833 1102 60-61 0.29805161742461195 1102 62-63 0.28220212567984504 1102 64-65 0.2893769068880161 1102 66-67 0.3802185034466703 1102 68-69 0.3343191981101796 1102 70-71 0.34627772174016513 1102 72-73 0.3179518049152179 1102 74-75 0.2390143818618924 1102 76-77 0.27893455439207315 1102 78-79 0.213062697008084 1102 80-81 0.18020278157059266 1102 82-83 0.19438359340678346 1102 84-85 0.15989334424288515 1102 86-87 0.206280351527937 1102 88-89 0.24862216128149583 1102 90-91 0.21532108246492854 1102 92-93 0.24045257266691422 1102 94-95 0.19946061818990302 1102 96-97 0.19217983189007626 1102 98-99 0.20060621676667267 1102 100-101 0.22944563113775374 1103 1 0.3036384089035131 1103 2 0.32374487346782743 1103 3 0.38647355099695346 1103 4 0.38116498601814897 1103 5 0.36121831746339694 1103 6 0.4318874402504136 1103 7 0.419819326891421 1103 8 0.46752618837151516 1103 9 0.5347429717091856 1103 10-11 0.5883810811281478 1103 12-13 0.5484773440693331 1103 14-15 0.5767135108532813 1103 16-17 0.611754985452329 1103 18-19 0.6491796843263202 1103 20-21 0.6822816924119977 1103 22-23 0.5957118920040045 1103 24-25 0.5495395512242709 1103 26-27 0.5785164542161354 1103 28-29 0.6303783037009865 1103 30-31 0.5733181698783198 1103 32-33 0.6671595030592314 1103 34-35 0.7527406558810128 1103 36-37 0.7093858099099677 1103 38-39 0.6829712926460516 1103 40-41 0.49391933537113175 1103 42-43 0.520250392756374 1103 44-45 0.5580257282462142 1103 46-47 0.500518397279528 1103 48-49 0.4640219626350941 1103 50-51 0.45933836848514886 1103 52-53 0.4760734321994562 1103 54-55 0.3628114712743411 1103 56-57 0.41344743839895415 1103 58-59 0.3949173951650238 1103 60-61 0.3720844082331567 1103 62-63 0.3748168171354891 1103 64-65 0.3301889820608821 1103 66-67 0.28963415368385625 1103 68-69 0.20474349429109395 1103 70-71 0.2930337450326874 1103 72-73 0.2673989710318736 1103 74-75 0.3063934418835288 1103 76-77 0.3713100409375869 1103 78-79 0.4275583238207119 1103 80-81 0.4081301436227349 1103 82-83 0.45551798834020474 1103 84-85 0.37491169898644827 1103 86-87 0.4074968943711923 1103 88-89 0.3884738666937757 1103 90-91 0.3877666659754304 1103 92-93 0.3396249820680062 1103 94-95 0.3188281113598137 1103 96-97 0.3357444685212734 1103 98-99 0.3504120773609323 1103 100-101 0.3545897769119115 1104 1 0.24494002311626417 1104 2 0.17983591037521407 1104 3 0.27228452648596146 1104 4 0.29919989487309095 1104 5 0.3307998818955795 1104 6 0.3224029692603949 1104 7 0.4031757758975587 1104 8 0.45139476187173955 1104 9 0.518292914510269 1104 10-11 0.41503236654234854 1104 12-13 0.47538196887949624 1104 14-15 0.5934987643488157 1104 16-17 0.5839195073501458 1104 18-19 0.5221712484386174 1104 20-21 0.5453801103403109 1104 22-23 0.5079712148176085 1104 24-25 0.4739483117215286 1104 26-27 0.5047948804005884 1104 28-29 0.693568498847533 1104 30-31 0.6535242040209752 1104 32-33 0.6560492614833109 1104 34-35 0.6652396276715109 1104 36-37 0.7069323116365993 1104 38-39 0.6797999898969991 1104 40-41 0.621495304820364 1104 42-43 0.6354764236696866 1104 44-45 0.45702865064609455 1104 46-47 0.5209957035534316 1104 48-49 0.4102786897909496 1104 50-51 0.40778084054450403 1104 52-53 0.41383775820432334 1104 54-55 0.39228947596907915 1104 56-57 0.38748187351588825 1104 58-59 0.3624557359547822 1104 60-61 0.4155682638207523 1104 62-63 0.3634623377217707 1104 64-65 0.3196500060661478 1104 66-67 0.42518834337099776 1104 68-69 0.4101558177246716 1104 70-71 0.5001590654913173 1104 72-73 0.4576497741777459 1104 74-75 0.4234060820056271 1104 76-77 0.4547573531979552 1104 78-79 0.4505184704205334 1104 80-81 0.48701551882781047 1104 82-83 0.4471121281553678 1104 84-85 0.406480986597213 1104 86-87 0.36599622641583807 1104 88-89 0.38667990769115335 1104 90-91 0.40609185360462163 1104 92-93 0.4305385995222828 1104 94-95 0.41846776201230895 1104 96-97 0.43899751392548225 1104 98-99 0.41088720916215493 1104 100-101 0.4777211013523761 1105 1 0.1606740933714832 1105 2 0.15974465813330596 1105 3 0.24253243091124688 1105 4 0.22583883316526254 1105 5 0.261522658185001 1105 6 0.22699691179249015 1105 7 0.2503145753602496 1105 8 0.3232422070340917 1105 9 0.32145184799968973 1105 10-11 0.3639726661138525 1105 12-13 0.3321851762156953 1105 14-15 0.2916624630663307 1105 16-17 0.37125497565575216 1105 18-19 0.395943170363914 1105 20-21 0.3928094198700123 1105 22-23 0.3868592844874996 1105 24-25 0.30084680485884974 1105 26-27 0.4375932512178835 1105 28-29 0.4810666227734828 1105 30-31 0.4644464257429064 1105 32-33 0.3772613741678299 1105 34-35 0.41021334387403385 1105 36-37 0.427607960945835 1105 38-39 0.36114055737187556 1105 40-41 0.36320118143472513 1105 42-43 0.43277112488613767 1105 44-45 0.3588969389911796 1105 46-47 0.4792413304248129 1105 48-49 0.33941440123575006 1105 50-51 0.28334938478608507 1105 52-53 0.33023717812223197 1105 54-55 0.4737234719317627 1105 56-57 0.33619901407885777 1105 58-59 0.3469328178341584 1105 60-61 0.1482515374949358 1105 62-63 0.2237150824181029 1105 64-65 0.15239978656548203 1105 66-67 0.21014035339673143 1105 68-69 0.33002878875058883 1105 70-71 0.3407472709889703 1105 72-73 0.3088010270199959 1105 74-75 0.2165053188187045 1105 76-77 0.2732398719399356 1105 78-79 0.2652171354062389 1105 80-81 0.2558475501657469 1105 82-83 0.31302602010850933 1105 84-85 0.29789879204082936 1105 86-87 0.3364338019278179 1105 88-89 0.2748801523312103 1105 90-91 0.34350724266485955 1105 92-93 0.39188950416518864 1105 94-95 0.3285705145881579 1105 96-97 0.3229108355521113 1105 98-99 0.2751504373602991 1105 100-101 0.3201520805879916 1106 1 0.19273000960371078 1106 2 0.2010692863797665 1106 3 0.29849976722275073 1106 4 0.18996779900508898 1106 5 0.11249659621176988 1106 6 0.17799912173887833 1106 7 0.19617947406980818 1106 8 0.2829172353800189 1106 9 0.26602778400746274 1106 10-11 0.3327089187079082 1106 12-13 0.2722274101710127 1106 14-15 0.40019281171386467 1106 16-17 0.33754389395137707 1106 18-19 0.3327332639424938 1106 20-21 0.40833927042850604 1106 22-23 0.2748058994061964 1106 24-25 0.21804279291063722 1106 26-27 0.213226001942445 1106 28-29 0.33656552964193054 1106 30-31 0.3455255730558946 1106 32-33 0.4161930101498399 1106 34-35 0.44866185937622305 1106 36-37 0.4677259073979272 1106 38-39 0.485798851997842 1106 40-41 0.41673924195143286 1106 42-43 0.4556963582404805 1106 44-45 0.4084728046891968 1106 46-47 0.4383651118458687 1106 48-49 0.4694716703755475 1106 50-51 0.4272544732875474 1106 52-53 0.5235899095449099 1106 54-55 0.3427930852429526 1106 56-57 0.3678882279714699 1106 58-59 0.38989599624920857 1106 60-61 0.4109335472287583 1106 62-63 0.36347345001819065 1106 64-65 0.2742749438372769 1106 66-67 0.38854665714668357 1106 68-69 0.37339589666643036 1106 70-71 0.3837004210136854 1106 72-73 0.3745315749031519 1106 74-75 0.2782319300960907 1106 76-77 0.3295386632043602 1106 78-79 0.3507149358212871 1106 80-81 0.36710939550798827 1106 82-83 0.39707612431496386 1106 84-85 0.393176456336203 1106 86-87 0.3179773266220778 1106 88-89 0.39327038845354423 1106 90-91 0.3303095479364657 1106 92-93 0.37301468586037956 1106 94-95 0.37548273249804964 1106 96-97 0.4703693046942732 1106 98-99 0.3485098889146627 1106 100-101 0.3902873306390582 1107 1 0.15419324704248893 1107 2 0.20017737666630708 1107 3 0.20484141863468608 1107 4 0.15849484953627524 1107 5 0.11858831159516114 1107 6 0.11325475256900575 1107 7 0.18513120112015713 1107 8 -0.08779142071838208 1107 9 0.06569200097000305 1107 10-11 0.053704909531816725 1107 12-13 0.10692779784697848 1107 14-15 -0.028931960359862785 1107 16-17 0.03849747125305214 1107 18-19 0.15201110673554297 1107 20-21 0.10078917362134376 1107 22-23 0.11134250270980317 1107 24-25 0.006824704825078243 1107 26-27 0.15654762805210254 1107 28-29 0.03132478000383543 1107 30-31 0.12872849185419 1107 32-33 0.12616829614250236 1107 34-35 0.14237849803860314 1107 36-37 0.4087828657028538 1107 38-39 0.31718664785464057 1107 40-41 -0.025064404153106068 1107 42-43 0.05020646662513428 1107 44-45 0.015018102295144331 1107 46-47 0.08439824413883912 1107 48-49 0.24048987226766627 1107 50-51 0.1542883293974029 1107 52-53 0.21361543997116428 1107 54-55 0.1367397415489222 1107 56-57 -0.1442362081402777 1107 58-59 -0.0352893867653421 1107 60-61 -0.21320882146870446 1107 62-63 -0.07212490900214164 1107 64-65 -0.2374218229648406 1107 66-67 0.015098803724526988 1107 68-69 0.10211023369847538 1107 70-71 0.14415919476589067 1107 72-73 0.1571826280612001 1107 74-75 0.09220657279537647 1107 76-77 0.11228600905998576 1107 78-79 0.15805449720078002 1107 80-81 0.14057215696335135 1107 82-83 0.2094217316680158 1107 84-85 0.15306932875296653 1107 86-87 0.1570540552381665 1107 88-89 0.14128140183297688 1107 90-91 0.13239316086531971 1107 92-93 0.0884494658433077 1107 94-95 0.12069270202437465 1107 96-97 0.101173987315331 1107 98-99 -2.0488875992441535E-4 1107 100-101 0.031606482440189154 1108 1 -0.12397703336425536 1108 2 -0.0771921931711681 1108 3 0.09432785309454417 1108 4 -0.01833452328816776 1108 5 0.06828574883419236 1108 6 0.06708764495743935 1108 7 0.10880442924774059 1108 8 0.21680275471837263 1108 9 0.27737206704387063 1108 10-11 0.1856144454510229 1108 12-13 0.22850029493384483 1108 14-15 0.28353948234528303 1108 16-17 0.25913224225455167 1108 18-19 0.26498223121290465 1108 20-21 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-0.5548492684192752 2215 5 -0.5395059553495614 2215 6 -0.7444966138788445 2215 7 -0.7167741171997548 2215 8 -0.6443700402687043 2215 9 -0.7649078356002192 2215 10-11 -0.7337406414245891 2215 12-13 -0.6075365939661452 2215 14-15 -0.8030730336469105 2215 16-17 -0.8535301767195591 2215 18-19 -0.768929550644053 2215 20-21 -3.5635907571839667 2215 22-23 -1.4381142953889068 2215 24-25 -1.0534675994596512 2215 26-27 -0.8741953072464739 2215 28-29 -0.7611237229367305 2215 30-31 -1.1818484921962238 2215 32-33 -1.0596503539058943 2215 34-35 -1.2703244781084067 2215 36-37 -1.2544208733468523 2215 38-39 -1.0312951953014604 2215 40-41 -1.192751718029342 2215 42-43 -1.4657035371525708 2215 44-45 -1.047351522787089 2215 46-47 -0.8736561759033421 2215 48-49 -0.9413565032908409 2215 50-51 -0.7635608777818419 2215 52-53 -0.7275144712243673 2215 54-55 -0.7146669626783364 2215 56-57 -0.5584938971987512 2215 58-59 -0.4774952620027264 2215 60-61 -0.4835499383029642 2215 62-63 -0.4447204957369877 2215 64-65 -0.5307204207084979 2215 66-67 -0.5842350689150564 2215 68-69 -0.4286305978699829 2215 70-71 -0.2592624058241171 2215 72-73 -0.3062069642749776 2215 74-75 -0.3484374518747586 2215 76-77 -0.3988331438099664 2215 78-79 -0.3162421093623138 2215 80-81 -0.4572233818064433 2215 82-83 -0.4903590558964197 2215 84-85 -0.5323658976970513 2215 86-87 -0.491133107920831 2215 88-89 -0.39200898159587894 2215 90-91 -0.44126887428921435 2215 92-93 -0.4080562572042261 2215 94-95 -0.43849360371432766 2215 96-97 -0.37555588366891257 2215 98-99 -0.4156179388891097 2215 100-101 -0.4841821248393785 2216 1 -0.36359273579936513 2216 2 -0.3585546396432413 2216 3 -0.4614366288319012 2216 4 -0.319323500493752 2216 5 -0.33241735243623793 2216 6 -0.29202342334240683 2216 7 -0.5396878944334986 2216 8 -1.5242710404673474 2216 9 -0.8362988257592932 2216 10-11 -0.740749287204423 2216 12-13 -0.7245858439215525 2216 14-15 -1.417395814696647 2216 16-17 -0.8720028247137037 2216 18-19 -0.7897260856510044 2216 20-21 -0.7926634706768922 2216 22-23 -0.7158870399609469 2216 24-25 -0.9361916688310998 2216 26-27 -1.2614489635162514 2216 28-29 -1.8300448631166546 2216 30-31 -1.1384239318225156 2216 32-33 -0.9336385677039445 2216 34-35 -2.235951411232236 2216 36-37 -2.0526016267260374 2216 38-39 -2.181650099686955 2216 40-41 -1.300999270623727 2216 42-43 -1.2154657834268008 2216 44-45 -1.0072416452960624 2216 46-47 -1.419421176944276 2216 48-49 -1.2982611593118634 2216 50-51 -0.85759394900073 2216 52-53 -0.6649188030323856 2216 54-55 -0.6920837514841338 2216 56-57 -0.8118505234625744 2216 58-59 -0.6706280903153825 2216 60-61 -0.7061369374505233 2216 62-63 -0.5983947060978494 2216 64-65 -0.7525754798930322 2216 66-67 -0.6082548315923191 2216 68-69 -0.7642590730189198 2216 70-71 -0.9573001490509938 2216 72-73 -0.7557386375302926 2216 74-75 -0.5852410202461229 2216 76-77 -0.6494519671344534 2216 78-79 -0.6470614123550362 2216 80-81 -0.6512498837597747 2216 82-83 -0.9463384521145493 2216 84-85 -0.6341873141887895 2216 86-87 -0.8186200931692866 2216 88-89 -0.6311936663934219 2216 90-91 -0.8020961871628245 2216 92-93 -0.9413636639355651 2216 94-95 -0.7301546033641699 2216 96-97 -0.6219597513780677 2216 98-99 -0.6509007334681023 2216 100-101 -0.611789698240667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 61.0 11 616.0 12 2314.0 13 4671.0 14 6445.0 15 7713.0 16 8660.0 17 10056.0 18 14010.0 19 20239.0 20 20649.0 21 22684.0 22 26559.0 23 30694.0 24 34844.0 25 38036.0 26 44324.0 27 52324.0 28 62362.0 29 72723.0 30 83958.0 31 98447.0 32 119262.0 33 149592.0 34 194905.0 35 275886.0 36 458208.0 37 723522.0 38 638828.0 39 113231.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.500853281232374 38.981060997006914 12.896967791333736 19.621117930426976 2 23.883788527125088 26.9996677067326 32.08636385992819 17.030179906214123 3 22.264781003727418 27.66161839739045 22.885527374983955 27.18807322389818 4 26.297920561595646 25.459240433734493 25.10968289966583 23.133156105004034 5 23.091964191995682 29.362113691913212 23.657304802891034 23.88861731320008 6 22.555258864114855 24.533940254885465 26.01746408571098 26.893336795288704 7 26.84855061230817 25.35747085528251 23.34782636071897 24.446152171690354 8 27.75920654917125 24.35501540096165 24.836231744149163 23.049546305717932 9 22.02078231509702 25.98763661196043 24.990789362845746 27.000791710096806 10-11 26.190097552603987 24.88644343305427 24.104373866963638 24.8190851473781 12-13 25.33514913353296 25.10922829590669 23.993248604650674 25.56237396590968 14-15 25.151328520897763 24.913774406478186 24.36634624565125 25.568550826972803 16-17 25.267883332351595 27.00508000715245 24.17761454447131 23.549422116024648 18-19 23.0483858662555 24.95242232101732 26.430062048457962 25.56912976426922 20-21 22.955952359003945 27.73695975451328 24.738435139064325 24.56865274741845 22-23 22.07657621193451 27.10493888234289 27.62245268141166 23.196032224310947 24-25 21.948325434597855 25.4695878570603 29.335818113157426 23.24626859518442 26-27 21.926157415145276 25.200157034700382 30.65841699848167 22.215268551672676 28-29 21.937199915810027 24.976014252331264 31.032100725748762 22.05468510610995 30-31 22.03996987930137 24.909125300667405 30.938451014295342 22.112453805735882 32-33 21.955758689593594 24.910585498789846 31.022099070712738 22.111556740903822 34-35 22.036685892279316 25.03980542666786 30.967115614024284 21.956393067028547 36-37 22.02551172666183 25.083528474175232 30.685838058407207 22.205121740755736 38-39 22.18765889271564 25.122722138012417 30.40611479129292 22.28350417797903 40-41 22.855217032359253 25.807047773558978 28.43125704840308 22.906478145678687 42-43 22.921094248723605 26.010762743454872 28.20962633468226 22.858516673139263 44-45 23.0830027202974 26.359020800854715 27.670546189372807 22.887430289475077 46-47 22.99798286711054 26.8147076536239 27.45441066835176 22.732898810913802 48-49 22.98453831218526 26.949812690520737 27.246507188565204 22.819141808728798 50-51 22.92056659965115 26.82176614896391 27.430256869840154 22.827410381544784 52-53 22.89274152619476 26.987048835390436 27.30425539122242 22.815954247192387 54-55 22.952215104873638 26.792060972041693 27.371977658293723 22.88374626479095 56-57 22.889197382553494 26.679152966060666 27.62947466113576 22.802174990250073 58-59 22.954180913168077 26.79558379565919 27.525642203629424 22.724593087543305 60-61 22.923730553732806 26.7312974550072 27.48062530220272 22.86434668905727 62-63 22.971738256548488 26.653118192476267 27.566478602773504 22.80866494820174 64-65 23.101229062404304 26.846377766529557 27.314701219529557 22.73769195153658 66-67 23.10190183402647 26.81202723753313 27.138339851042208 22.94773107739819 68-69 23.106678421534312 26.81686597682261 27.077498491422013 22.99895711022107 70-71 23.217081080303295 27.053267535563354 26.82683539386033 22.902815990273027 72-73 23.214643571733593 27.0039904665277 26.689225800330384 23.092140161408324 74-75 23.171709655960736 26.964556428488564 26.836159631723334 23.02757428382737 76-77 23.267849557487637 27.131390333886017 26.679824775716092 22.92093533291025 78-79 23.250916239547855 27.03530182696337 26.62586430481804 23.08791762867073 80-81 23.242159455163033 26.989277546638878 26.72661955689879 23.0419434412993 82-83 23.37169701197375 27.114801157475345 26.52572139114075 22.98778043941016 84-85 23.393705430251238 27.037359675268284 26.545127090763188 23.023807803717293 86-87 23.451710366216485 26.957636612116048 26.59040691286939 23.000246108798077 88-89 23.452909434660214 27.097148225543577 26.474801726538654 22.97514061325756 90-91 23.53101375541342 27.123872463483117 26.284614483008152 23.060499298095312 92-93 23.449567031373324 27.023300597765598 26.449966021397977 23.077166349463102 94-95 23.609822528877313 27.211434436586217 26.19730731194935 22.981435722587122 96-97 23.590982165955268 27.197675126194458 26.19156677177501 23.019775936075263 98-99 23.686128246964934 27.127184890058913 26.257050897157995 22.929635965818157 100-101 23.82670403363627 27.3095482119941 25.938968015748564 22.924779738621066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2468.0 1 1949.0 2 1215.5 3 880.0 4 639.5 5 452.5 6 367.5 7 300.5 8 241.0 9 223.0 10 195.0 11 165.0 12 158.5 13 187.5 14 221.0 15 283.0 16 1673.0 17 1955.0 18 1003.0 19 1241.5 20 1689.0 21 2369.0 22 3249.5 23 4497.5 24 5920.5 25 7774.0 26 10292.0 27 13626.0 28 17550.5 29 22640.0 30 29524.5 31 36350.0 32 44122.5 33 54823.0 34 65998.5 35 76958.0 36 86171.0 37 92489.0 38 97874.5 39 103597.5 40 106570.0 41 106244.5 42 106207.0 43 109229.0 44 114063.5 45 118671.5 46 122029.5 47 124891.5 48 127087.0 49 126044.5 50 123764.0 51 122069.0 52 122444.5 53 122105.5 54 116623.0 55 109426.5 56 101823.5 57 91323.5 58 82273.5 59 74882.5 60 65733.0 61 55867.0 62 45610.0 63 36029.5 64 28048.5 65 20999.0 66 15349.5 67 11178.0 68 8196.5 69 6325.0 70 5212.0 71 4257.0 72 3626.0 73 2871.0 74 2076.0 75 1640.5 76 1210.0 77 763.5 78 505.0 79 301.5 80 120.5 81 54.5 82 31.5 83 12.0 84 5.5 85 3.5 86 1.0 87 0.0 88 3.0 89 3.5 90 1.0 91 1.0 92 0.5 93 0.5 94 1.5 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08714229914779208 2 0.04553462415049748 3 0.05129015268038261 4 0.059773561919744554 5 0.0441556954402125 6 0.018615537588847226 7 0.02266239358642271 8 0.012500288525844271 9 0.003177531375874083 10-11 9.142896883411279E-4 12-13 5.995342218630347E-4 14-15 0.001543800621297314 16-17 7.494177773287933E-5 18-19 0.0018735444433219833 20-21 0.005470749774500191 22-23 0.01657712123451291 24-25 0.02051905874326236 26-27 0.010626744082522288 28-29 0.018255817055729404 30-31 3.2974382202466905E-4 32-33 0.005830470307618012 34-35 2.5480204429178974E-4 36-37 0.0025330320873713213 38-39 0.010162105060578438 40-41 0.0018285793766822558 42-43 0.001903521154415135 44-45 0.002233264976439804 46-47 0.0 48-49 0.0 50-51 0.0038520073754699977 52-53 0.0025180437318247455 54-55 0.0029676943982220212 56-57 0.0 58-59 0.0048712155526371564 60-61 0.0 62-63 0.0 64-65 0.0026229622206507767 66-67 3.447321775712449E-4 68-69 0.0 70-71 0.001348951999191828 72-73 0.002922729331582294 74-75 0.005096040885835794 76-77 0.0025180437318247455 78-79 1.4988355546575866E-4 80-81 7.793944884219449E-4 82-83 0.0012590218659123728 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3335923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.209129450416604 #Duplication Level Percentage of deduplicated Percentage of total 1 85.18952884617839 53.84755956658089 2 8.314189700707427 10.510653861346725 3 2.3858812324543983 4.524284270265887 4 1.0948751864434736 2.7682442956781816 5 0.629576267232517 1.9897483887205119 6 0.41803833124196677 1.5854303396825764 7 0.29124230654263245 1.2886420858983818 8 0.20151949803609703 1.0190297630517304 9 0.15870103588464307 0.9028218889032852 >10 1.1979258542675657 15.689841482327719 >50 0.1072356035266658 4.471929289374073 >100 0.010905207539183847 0.9242828710828457 >500 2.3808121565919768E-4 0.11518432942360159 >1k 9.52324862211945E-5 0.15419109470391837 >5k 4.761624311059725E-5 0.20815647295965245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 6916 0.20731893392023737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.997671109315173E-5 0.0 0.0 0.0 0.0 3 5.995342218630346E-5 0.0 0.0 0.0 2.997671109315173E-5 4 8.99301332794552E-5 0.0 0.0 0.0 2.997671109315173E-5 5 2.0983697765206212E-4 0.0 0.0 0.0 2.997671109315173E-5 6 2.0983697765206212E-4 0.0 0.0 0.0 2.997671109315173E-5 7 2.697903998383656E-4 0.0 0.0 0.0 2.997671109315173E-5 8 3.2974382202466905E-4 0.0 0.0 0.0 2.997671109315173E-5 9 4.1967395530412424E-4 0.0 0.0 0.0 2.997671109315173E-5 10-11 7.344294217822174E-4 0.0 0.0 0.0 4.49650666397276E-5 12-13 0.0010341965327137347 0.0 0.0 0.0 5.995342218630346E-5 14-15 0.001229045154819221 2.997671109315173E-5 0.0 0.0 5.995342218630346E-5 16-17 0.001663707465669921 2.997671109315173E-5 0.0 0.0 8.99301332794552E-5 18-19 0.0021882999098000763 2.997671109315173E-5 0.0 0.0 8.99301332794552E-5 20-21 0.0027128923539302316 2.997671109315173E-5 0.0 0.0 8.99301332794552E-5 22-23 0.0034772984868056004 2.997671109315173E-5 0.0 0.0 8.99301332794552E-5 24-25 0.005170982663568673 4.49650666397276E-5 0.0 0.0 8.99301332794552E-5 26-27 0.006459981240574198 5.995342218630346E-5 0.0 0.0 1.0491848882603106E-4 28-29 0.008528374306001667 5.995342218630346E-5 0.0 0.0 1.798602665589104E-4 30-31 0.01047686052705653 5.995342218630346E-5 0.0 0.0 1.798602665589104E-4 32-33 0.013009892614427851 5.995342218630346E-5 0.0 0.0 1.798602665589104E-4 34-35 0.015393041146333414 5.995342218630346E-5 0.0 0.0 1.798602665589104E-4 36-37 0.018180875277996528 8.99301332794552E-5 0.0 0.0 1.798602665589104E-4 38-39 0.022242719631118582 1.348951999191828E-4 0.0 0.0 1.798602665589104E-4 40-41 0.027278807094768077 1.4988355546575866E-4 0.0 0.0 1.798602665589104E-4 42-43 0.03448820611267107 1.6487191101233453E-4 0.0 0.0 2.0983697765206212E-4 44-45 0.044485439262237164 1.798602665589104E-4 0.0 0.0 2.3981368874521385E-4 46-47 0.05560679907779646 1.9484862210548625E-4 0.0 0.0 2.8477875538494146E-4 48-49 0.06966587658048462 2.0983697765206212E-4 0.0 0.0 2.997671109315173E-4 50-51 0.08552355674876189 2.2482533319863798E-4 0.0 0.0 3.2974382202466905E-4 52-53 0.1052632210036023 2.3981368874521385E-4 0.0 2.997671109315173E-5 3.447321775712449E-4 54-55 0.1272511385904291 2.3981368874521385E-4 0.0 5.995342218630346E-5 3.597205331178208E-4 56-57 0.1541402484409862 2.3981368874521385E-4 0.0 5.995342218630346E-5 3.597205331178208E-4 58-59 0.18470150540045438 2.3981368874521385E-4 0.0 5.995342218630346E-5 3.597205331178208E-4 60-61 0.21933959506859121 2.3981368874521385E-4 0.0 5.995342218630346E-5 4.1967395530412424E-4 62-63 0.25806950580094323 2.3981368874521385E-4 0.0 5.995342218630346E-5 4.4965066639727596E-4 64-65 0.3028097471074722 2.5480204429178974E-4 0.0 5.995342218630346E-5 4.4965066639727596E-4 66-67 0.3534404121438055 2.697903998383656E-4 0.0 5.995342218630346E-5 4.4965066639727596E-4 68-69 0.41078586046500476 2.697903998383656E-4 0.0 5.995342218630346E-5 4.796273774904277E-4 70-71 0.478098565224677 2.8477875538494146E-4 0.0 5.995342218630346E-5 4.796273774904277E-4 72-73 0.5540895278458166 2.997671109315173E-4 0.0 5.995342218630346E-5 4.796273774904277E-4 74-75 0.6376795867290701 3.2974382202466905E-4 0.0 5.995342218630346E-5 5.096040885835794E-4 76-77 0.7313717972507159 3.896972442109725E-4 0.0 5.995342218630346E-5 5.245924441301553E-4 78-79 0.8335174403006305 3.896972442109725E-4 0.0 5.995342218630346E-5 5.695575107698829E-4 80-81 0.950681415608214 3.896972442109725E-4 0.0 5.995342218630346E-5 5.995342218630347E-4 82-83 1.0810351437967842 3.896972442109725E-4 0.0 5.995342218630346E-5 6.444992885027622E-4 84-85 1.2201120949134618 4.1967395530412424E-4 0.0 5.995342218630346E-5 6.594876440493381E-4 86-87 1.3716143927782505 4.1967395530412424E-4 0.0 7.494177773287933E-5 6.594876440493381E-4 88-89 1.5411626707211168 4.1967395530412424E-4 0.0 8.99301332794552E-5 6.594876440493381E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 14275 0.0 89.74584 1 TATCTCG 6980 0.0 85.99156 2 ATATCTC 7280 0.0 82.53675 1 ATCTCGA 7395 0.0 81.10032 3 TCGACGG 16115 0.0 79.594025 2 CGACGGC 16585 0.0 77.22264 3 TCTCGAG 5620 0.0 76.694786 4 TCGAGGG 5760 0.0 74.80151 6 CGGCGCG 17215 0.0 74.3389 6 ACGGCGC 17255 0.0 74.18325 5 GACGGCG 17320 0.0 73.97191 4 CGAGGGC 5860 0.0 73.52282 7 AGGGCGC 5840 0.0 73.51848 9 CTCGAGG 5895 0.0 73.02434 5 CTCGACG 2310 0.0 72.402565 5 GAGGGCG 6610 0.0 65.24855 8 TCTCGAC 2595 0.0 64.64459 4 CGCGCCG 22555 0.0 56.93834 9 GGCGCGC 22985 0.0 56.00636 7 GCGCGCC 23025 0.0 55.926334 8 >>END_MODULE