FastQCFastQC Report
Tue 31 May 2016
SRR2048249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335923
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT193160.5790301514753188No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT165100.49491550014793506No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT55070.1650817479899866No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41070.12311435245957415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG40500.072.39061
TCGACGG45050.066.661392
ATATCTC41900.065.208751
TATCTCG46400.058.7822232
CGACGGC52250.057.5664563
GACGGCG60900.049.545994
ACGGCGC61350.049.085985
CGGCGCG62800.047.9526256
GGTCGAC3600.043.5575561
ATCTCGA69250.039.523443
TCTCGAG68850.037.2684974
CTCGAGG75050.034.113075
TCGAGGG76200.033.660586
CGAGGGC78650.032.672427
AGGGCGC82050.031.2027579
GAGGGCG87000.029.6458138
GGGCGCG82700.027.6262788
CGCGCCG141950.024.4604689
GGCGCGC146900.023.7655757
GCGCGCC147000.023.7494098
Produced by FastQC (version 0.11.5)