FastQCFastQC Report
Tue 31 May 2016
SRR2048248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10151377
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT933550.919628933099421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA463560.45664740852398644No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT111260.1096008945387409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG319350.088.02942
GTCGACG288700.087.6659851
ATATCTC340500.082.813061
ATCTCGA355050.079.054313
CTCGACG139150.078.60885
TCTCGAG230000.074.427684
TCTCGAC148350.073.768754
TCGAGGG233850.072.955736
CTCGAGG235850.072.4780355
CGAGGGC236500.072.138257
AGGGCGC245100.069.781499
GAGGGCG248000.068.8314368
TCGACGG416700.061.1359142
CGACGGC431000.059.05093
GACGGCG458150.055.5692674
ACGGCGC463350.054.974225
CGGCGCG473600.053.754346
CGCGCCG724450.035.9738589
GGCGCGC728600.035.8145877
GCGCGCC729000.035.768878
Produced by FastQC (version 0.11.5)