FastQCFastQC Report
Tue 31 May 2016
SRR2048248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10151377
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT855790.8430284876623142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA744660.7335556545678482No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT511550.5039217832221186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143690.14154729944518857No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT132410.13043550643425025No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106610.1050202351858275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG178250.076.318982
ATATCTC188250.072.739871
GTCGACG89800.071.001481
ATCTCGA273600.049.7043463
TCGACGG137100.048.8160482
TCTCGAG233100.048.1108254
CGACGGC149500.044.7670943
TCGAGGG250350.044.568146
CTCGAGG251450.044.486515
CGAGGGC252100.044.2587627
GAGGGCG268400.041.5178228
AGGGCGC272450.040.918099
CTCGACG59800.040.112465
GACGGCG177900.037.700564
ACGGCGC181000.037.133595
TCTCGAC67800.035.4494824
CGGCGCG198500.033.811996
GGTCGAC8750.032.6030461
GGGCGCG271400.025.744948
GGATCCT316650.020.46086518-19
Produced by FastQC (version 0.11.5)