FastQCFastQC Report
Tue 31 May 2016
SRR2048247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10384367
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT698960.6730886918769339No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391360.37687419945770406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG310650.087.5148541
TATCTCG232050.085.339772
ATATCTC256300.077.432121
ATCTCGA258100.076.636193
TCGACGG367400.074.2685552
TCTCGAG202700.073.530684
CGAGGGC210750.070.6305167
CGACGGC387000.070.553243
TCGAGGG212200.070.12556
AGGGCGC212350.070.0536049
CTCGAGG214500.069.440015
CTCGACG70950.068.751015
GAGGGCG223250.066.654578
ACGGCGC412850.066.1510245
CGGCGCG413450.066.043546
GACGGCG414450.065.862534
TCTCGAC78000.062.720864
CGCGCCG615750.045.2170689
GCGCGCC627850.044.3758968
GGCGCGC629900.044.246567
Produced by FastQC (version 0.11.5)