FastQCFastQC Report
Tue 31 May 2016
SRR2048247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10384367
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT565760.5448189571882427No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT486700.46868528433172674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470470.453056021614028No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239510.2306447759406038No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT136690.1316305558152943No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113230.10903890434534912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG86500.069.410341
ATATCTC133500.064.09561
TCGACGG106450.057.9186822
TATCTCG147150.057.8452152
CGACGGC124750.049.384323
ACGGCGC146450.042.1641775
GACGGCG146800.042.0312924
ATCTCGA209450.040.6620833
CGGCGCG153400.040.2229086
TCTCGAG196600.038.342734
CGAGGGC220100.034.2920577
TCGAGGG219850.034.2662356
CTCGAGG220650.034.2281075
AGGGCGC231700.032.6162349
GGTCGAC9600.032.186891
GAGGGCG236050.032.0151758
GGGCGCG226800.030.053748
CTCGACG33200.029.6156965
TCTCGAC39650.025.15744
CGCGCCG375100.020.7169539
Produced by FastQC (version 0.11.5)