FastQCFastQC Report
Tue 31 May 2016
SRR2048246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10862499
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT188280.17333028062879455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG328600.091.554851
TCGACGG336650.089.4913252
CGACGGC349450.086.158983
ACGGCGC365450.082.425795
GACGGCG365850.082.335674
CGGCGCG366100.082.019976
TATCTCG72850.077.8465962
CGCGCCG440100.068.6497349
GGCGCGC443700.068.103457
GCGCGCC445200.067.863328
ATCTCGA92200.061.4574283
TCTCGAG87800.058.316184
TCGAGGG87500.058.081876
AGGGCGC88100.057.7402159
CGAGGGC90950.055.8786547
ATATCTC103800.055.1849671
CTCGAGG93050.054.9748655
GAGGGCG97100.052.535148
CTCGACG12650.042.8034865
TCCATAT416300.036.2827818-19
Produced by FastQC (version 0.11.5)