FastQCFastQC Report
Tue 31 May 2016
SRR2048246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10862499
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT511840.4711991227801264No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141950.13067895334213608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG53600.083.816321
TCGACGG58250.078.528622
ATCTCGA127600.064.9766541
CGACGGC72100.063.7060663
TCTCGAG135250.059.810162
ACGGCGC91000.050.5792055
TCGAGGG161800.050.0829734
GACGGCG92250.049.945344
CTCGAGG164200.049.3509453
CGAGGGC164900.049.141455
GGGCGCG169250.047.738118
CGGCGCG97700.047.1592376
AGGGCGC177250.045.7442867
GAGGGCG178100.045.525976
TATCTCG31800.040.6293072
GGCGCGC272250.030.4101879
CGCGCCG267600.026.1283079
GCGCGCC272650.025.7140488
CTCGACG8300.022.8913543
ATATCTC60000.022.5816251
Produced by FastQC (version 0.11.5)