##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10862499 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.362935453434794 33.0 31.0 34.0 31.0 34.0 2 32.46425256287711 34.0 31.0 34.0 31.0 34.0 3 32.609645165444896 34.0 31.0 34.0 31.0 34.0 4 36.223972402667194 37.0 35.0 37.0 35.0 37.0 5 36.08029211326049 37.0 35.0 37.0 35.0 37.0 6 36.033533075584174 37.0 35.0 37.0 35.0 37.0 7 35.98693357762335 37.0 35.0 37.0 35.0 37.0 8 35.966673230533786 37.0 35.0 37.0 35.0 37.0 9 37.64925179739947 39.0 37.0 39.0 35.0 39.0 10-11 37.693325173148466 39.0 37.0 39.0 35.0 39.0 12-13 37.70500020299196 39.0 37.0 39.0 35.0 39.0 14-15 39.106015015513464 40.0 38.0 41.0 36.0 41.0 16-17 39.10830587878535 40.0 38.0 41.0 36.0 41.0 18-19 39.14650634260127 40.0 38.5 41.0 36.0 41.0 20-21 39.17743090241021 40.0 39.0 41.0 36.0 41.0 22-23 39.16557051006403 40.0 39.0 41.0 36.0 41.0 24-25 39.130847560952596 40.0 39.0 41.0 36.0 41.0 26-27 39.04709464184991 40.0 38.5 41.0 36.0 41.0 28-29 38.94972823472757 40.0 38.0 41.0 35.0 41.0 30-31 38.84826502630749 40.0 38.0 41.0 35.0 41.0 32-33 38.765863775913814 40.0 38.0 41.0 35.0 41.0 34-35 38.70239426489245 40.0 38.0 41.0 35.0 41.0 36-37 38.65302496230379 40.0 38.0 41.0 35.0 41.0 38-39 38.61603154117667 40.0 38.0 41.0 35.0 41.0 40-41 38.664220590492114 40.0 38.0 41.0 35.0 41.0 42-43 38.62986445384253 40.0 38.0 41.0 35.0 41.0 44-45 38.55811056921616 40.0 38.0 41.0 34.0 41.0 46-47 38.491913002707754 40.0 38.0 41.0 34.0 41.0 48-49 38.39881057756599 40.0 38.0 41.0 34.0 41.0 50-51 38.29519731141057 40.0 38.0 41.0 34.0 41.0 52-53 38.18669419440223 40.0 37.0 41.0 34.0 41.0 54-55 38.02159701004345 40.0 37.0 41.0 33.0 41.0 56-57 37.821235012311625 40.0 37.0 41.0 33.0 41.0 58-59 37.631440748579124 39.5 36.5 41.0 33.0 41.0 60-61 37.393183235275785 39.0 36.0 41.0 33.0 41.0 62-63 37.24524499380851 39.0 36.0 41.0 33.0 41.0 64-65 37.0290932132652 39.0 35.0 40.5 32.5 41.0 66-67 36.744917076632184 38.0 35.0 40.0 32.0 41.0 68-69 36.41058820810939 37.5 35.0 40.0 32.0 41.0 70-71 36.0478887040634 37.0 35.0 39.0 32.0 41.0 72-73 35.67870321553079 36.5 35.0 39.0 31.5 41.0 74-75 35.302197680294384 36.0 35.0 39.0 31.0 40.0 76-77 34.93057840557684 35.5 34.5 37.5 31.0 39.0 78-79 34.578658235089364 35.0 34.0 37.0 31.0 39.0 80-81 34.274602188686046 35.0 34.0 36.5 31.0 38.5 82-83 34.001846122149246 35.0 34.0 36.0 31.0 37.0 84-85 33.76009502969805 35.0 34.0 36.0 31.0 37.0 86-87 33.562925805562784 35.0 34.0 35.0 31.0 36.5 88-89 33.40952174080753 35.0 34.0 35.0 30.5 36.0 90-91 33.27002147480059 35.0 34.0 35.0 30.0 36.0 92-93 33.130097779525684 35.0 34.0 35.0 30.0 36.0 94-95 33.02243010563223 35.0 34.0 35.0 30.0 35.5 96-97 32.90608726408168 35.0 34.0 35.0 30.0 35.0 98-99 32.81662451706555 35.0 34.0 35.0 30.0 35.0 100-101 32.232910953547616 34.5 33.0 35.0 28.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5269056342542768 1101 2 0.6242198893436992 1101 3 0.5356644520788478 1101 4 0.3267314923377782 1101 5 0.3629800834186412 1101 6 0.3486036661771905 1101 7 0.38584362597738675 1101 8 0.369847135629918 1101 9 0.4453452650186094 1101 10-11 0.4074440097294669 1101 12-13 0.4342558976720099 1101 14-15 0.5959245286470605 1101 16-17 0.5579792419429026 1101 18-19 0.5444620635034099 1101 20-21 0.559559520859878 1101 22-23 0.5554416049178883 1101 24-25 0.5913921223781102 1101 26-27 0.5777667765454595 1101 28-29 0.6262273202263202 1101 30-31 0.6314386636343912 1101 32-33 0.6416392095805605 1101 34-35 0.6549125223110082 1101 36-37 0.6833012093450037 1101 38-39 0.6537043276928571 1101 40-41 0.6060504386272356 1101 42-43 0.5945131245313036 1101 44-45 0.5992399517359175 1101 46-47 0.6111368580171757 1101 48-49 0.6228956442789766 1101 50-51 0.6230547042472807 1101 52-53 0.5898950514817543 1101 54-55 0.5654385311878443 1101 56-57 0.5834186303854167 1101 58-59 0.5833434343950543 1101 60-61 0.5716520839501698 1101 62-63 0.5572470434959911 1101 64-65 0.5251181874596753 1101 66-67 0.49045931748253935 1101 68-69 0.49742746434397844 1101 70-71 0.4959344294947954 1101 72-73 0.4665521629172389 1101 74-75 0.4255848432706344 1101 76-77 0.4102374524154655 1101 78-79 0.43273554000879244 1101 80-81 0.39446147023193845 1101 82-83 0.38861532860885717 1101 84-85 0.40359370676771533 1101 86-87 0.3937250837112245 1101 88-89 0.3788148376734526 1101 90-91 0.4264006852608375 1101 92-93 0.4444339201360634 1101 94-95 0.4495538895306197 1101 96-97 0.4780734390998589 1101 98-99 0.483155772458403 1101 100-101 0.5979359680404883 1102 1 0.5161491998558105 1102 2 0.5965900468299665 1102 3 0.4981071780122903 1102 4 0.30569616067563743 1102 5 0.3245034335879211 1102 6 0.3376533395240031 1102 7 0.33567890886422447 1102 8 0.3302756588433482 1102 9 0.4032665157296691 1102 10-11 0.3768297737446531 1102 12-13 0.38041274706244366 1102 14-15 0.5111513542763078 1102 16-17 0.4899654509266611 1102 18-19 0.47918045823276856 1102 20-21 0.5089323957524599 1102 22-23 0.5184418367524728 1102 24-25 0.5100091793676853 1102 26-27 0.5169678227901713 1102 28-29 0.5293094478502809 1102 30-31 0.5719811666689694 1102 32-33 0.594925221401283 1102 34-35 0.5858709086613416 1102 36-37 0.5903573654834346 1102 38-39 0.5556614998351179 1102 40-41 0.5504306306848861 1102 42-43 0.5543858492322258 1102 44-45 0.5174187399466703 1102 46-47 0.48334956230824133 1102 48-49 0.5212997137946616 1102 50-51 0.5405806212277184 1102 52-53 0.5268524229248399 1102 54-55 0.572482590026894 1102 56-57 0.5426680379777409 1102 58-59 0.5417609971862518 1102 60-61 0.5430637318186555 1102 62-63 0.49135678503738944 1102 64-65 0.49415354279422274 1102 66-67 0.420005448172013 1102 68-69 0.44388875267740246 1102 70-71 0.44779151211071166 1102 72-73 0.40652031086247575 1102 74-75 0.3618876266004989 1102 76-77 0.37950692728332314 1102 78-79 0.3597215466852077 1102 80-81 0.37969221051244517 1102 82-83 0.3670095724906943 1102 84-85 0.3702723467806166 1102 86-87 0.3496185722832621 1102 88-89 0.37002959689139203 1102 90-91 0.36627338401071796 1102 92-93 0.4141546250619257 1102 94-95 0.4107969953556534 1102 96-97 0.4031307501545527 1102 98-99 0.4705820420296192 1102 100-101 0.5290270828897761 1103 1 0.41405521927227085 1103 2 0.5050299504279749 1103 3 0.4182575893498637 1103 4 0.27365649075040466 1103 5 0.2925133750712092 1103 6 0.29271357221713856 1103 7 0.25256490957443845 1103 8 0.24052105987549055 1103 9 0.31277122686135783 1103 10-11 0.3061003744269115 1103 12-13 0.30331949468526886 1103 14-15 0.39409020896887625 1103 16-17 0.41052086125176857 1103 18-19 0.4166936279889768 1103 20-21 0.4021082150623201 1103 22-23 0.44991849536967266 1103 24-25 0.40873410820023537 1103 26-27 0.4005367953258343 1103 28-29 0.43303759620725657 1103 30-31 0.44118117532722323 1103 32-33 0.4637599596799049 1103 34-35 0.45907318993761237 1103 36-37 0.47290983055847846 1103 38-39 0.5007801427001155 1103 40-41 0.4103475890268129 1103 42-43 0.4062135989482272 1103 44-45 0.40501123750849644 1103 46-47 0.39838686358248054 1103 48-49 0.386243946911641 1103 50-51 0.37138372468029957 1103 52-53 0.32007625685338326 1103 54-55 0.3646841541389563 1103 56-57 0.3575813840526365 1103 58-59 0.344918319490489 1103 60-61 0.3194200283897075 1103 62-63 0.4071746787246795 1103 64-65 0.3363398543670755 1103 66-67 0.29883524805840267 1103 68-69 0.300402005857741 1103 70-71 0.28530379636296743 1103 72-73 0.2948198046800954 1103 74-75 0.3270259235797468 1103 76-77 0.2930149356607288 1103 78-79 0.25985872271546384 1103 80-81 0.22903889158849466 1103 82-83 0.23544844099537698 1103 84-85 0.21504753067591054 1103 86-87 0.2724962543199396 1103 88-89 0.22917583622065507 1103 90-91 0.2427165032101115 1103 92-93 0.29847920413482854 1103 94-95 0.3251804337445563 1103 96-97 0.28914367912638994 1103 98-99 0.2870661752903345 1103 100-101 0.2901134153136482 1104 1 0.3698262182092549 1104 2 0.4651357045097342 1104 3 0.37453567777647123 1104 4 0.2523084780731608 1104 5 0.2680668596666109 1104 6 0.24219154103117546 1104 7 0.2505740462572774 1104 8 0.2527742444791983 1104 9 0.30410448138116664 1104 10-11 0.29222738182046015 1104 12-13 0.30753924795467213 1104 14-15 0.3826536475321731 1104 16-17 0.4133460165957956 1104 18-19 0.4013792563272034 1104 20-21 0.40692079622613875 1104 22-23 0.40488950165315174 1104 24-25 0.4075230754111345 1104 26-27 0.43923452216127856 1104 28-29 0.44182266688837046 1104 30-31 0.4130942206161379 1104 32-33 0.4328332202038041 1104 34-35 0.46828030290588885 1104 36-37 0.4302012339613981 1104 38-39 0.45618270918871673 1104 40-41 0.4026514536353858 1104 42-43 0.41335711652434526 1104 44-45 0.4235720153130629 1104 46-47 0.41870269361947976 1104 48-49 0.4309293035209478 1104 50-51 0.44361819880688813 1104 52-53 0.44167253120610184 1104 54-55 0.4480279995079073 1104 56-57 0.43975711906362847 1104 58-59 0.38835058642532516 1104 60-61 0.3969395976240193 1104 62-63 0.38013702471183564 1104 64-65 0.39598937756583297 1104 66-67 0.3417343279866216 1104 68-69 0.319470809669383 1104 70-71 0.33289379041418954 1104 72-73 0.31530922524813576 1104 74-75 0.3145601155698827 1104 76-77 0.33305340681051376 1104 78-79 0.28590748526332277 1104 80-81 0.2726023450249855 1104 82-83 0.2556218278068698 1104 84-85 0.2866472650517906 1104 86-87 0.24551151740068633 1104 88-89 0.2566933173450323 1104 90-91 0.2710912434106021 1104 92-93 0.2701651771114868 1104 94-95 0.30337069635997693 1104 96-97 0.29320103224551985 1104 98-99 0.35447668192881565 1104 100-101 0.4250499565820576 1105 1 0.333900038435452 1105 2 0.4395452421411221 1105 3 0.34657591391682985 1105 4 0.20688554154394012 1105 5 0.21087228112921252 1105 6 0.20019582641925382 1105 7 0.21917663238204455 1105 8 0.18353773537933193 1105 9 0.2861880786628035 1105 10-11 0.28544278474012685 1105 12-13 0.27614713899205867 1105 14-15 0.3660979238576445 1105 16-17 0.3482964737699277 1105 18-19 0.33898975388638775 1105 20-21 0.34702086671681087 1105 22-23 0.3543496641732631 1105 24-25 0.34314181982423975 1105 26-27 0.39189562625477237 1105 28-29 0.4094857186101848 1105 30-31 0.3816041774843342 1105 32-33 0.38544905459897905 1105 34-35 0.446843305447544 1105 36-37 0.39889082035036694 1105 38-39 0.42544143259127765 1105 40-41 0.39113249689735596 1105 42-43 0.3712550113823667 1105 44-45 0.3812721518723876 1105 46-47 0.37185964424654117 1105 48-49 0.3701974522524907 1105 50-51 0.3756446435676679 1105 52-53 0.390808641252967 1105 54-55 0.33222537776708094 1105 56-57 0.33237986314158263 1105 58-59 0.3309936060822736 1105 60-61 0.34394959167354955 1105 62-63 0.2923914437540205 1105 64-65 0.2656143289356905 1105 66-67 0.21937609269780722 1105 68-69 0.17197276759556956 1105 70-71 0.19664681380550064 1105 72-73 0.21844430867098197 1105 74-75 0.21538984873075862 1105 76-77 0.21113225361634136 1105 78-79 0.19393110700492144 1105 80-81 0.15359702653129403 1105 82-83 0.15108268953959936 1105 84-85 0.12775459776340625 1105 86-87 0.13655708505849873 1105 88-89 0.17550967100438442 1105 90-91 0.14708508085578842 1105 92-93 0.18449094570503632 1105 94-95 0.2056038371144311 1105 96-97 0.23121509539171115 1105 98-99 0.2588994658800914 1105 100-101 0.3044421440749403 1106 1 0.2484162192112862 1106 2 0.35896205226578815 1106 3 0.2587183955090495 1106 4 0.1722149422811583 1106 5 0.18369698744888296 1106 6 0.17125505532793284 1106 7 0.15507641331245026 1106 8 0.16044381443972355 1106 9 0.21128829981149977 1106 10-11 0.1858333451459515 1106 12-13 0.18694235985838503 1106 14-15 0.25338743478627634 1106 16-17 0.2664252836023593 1106 18-19 0.26496092209285393 1106 20-21 0.20678729079586589 1106 22-23 0.25757912874372835 1106 24-25 0.2700813748346462 1106 26-27 0.2482317627002999 1106 28-29 0.2932382882344555 1106 30-31 0.28037994093519814 1106 32-33 0.27058707634948576 1106 34-35 0.2984994299822432 1106 36-37 0.2997294240848589 1106 38-39 0.28737289022702583 1106 40-41 0.27499933248375896 1106 42-43 0.2656982629625304 1106 44-45 0.2856346451104699 1106 46-47 0.2684612146111718 1106 48-49 0.29717116738233784 1106 50-51 0.30245414595070486 1106 52-53 0.28128676543160935 1106 54-55 0.2802601996451486 1106 56-57 0.23751279339034426 1106 58-59 0.2101784050515363 1106 60-61 0.24043578399855647 1106 62-63 0.2049060844959385 1106 64-65 0.21997813490873597 1106 66-67 0.24121134741093186 1106 68-69 0.18448242453803942 1106 70-71 0.17448787503784047 1106 72-73 0.19546888744503832 1106 74-75 0.1505258681373718 1106 76-77 0.1381977121171829 1106 78-79 0.16628315621561285 1106 80-81 0.1578774248793806 1106 82-83 0.15818527462658238 1106 84-85 0.1519131576269217 1106 86-87 0.194770506823275 1106 88-89 0.17900844508400837 1106 90-91 0.17442745813513483 1106 92-93 0.12162571588599747 1106 94-95 0.16529564562899424 1106 96-97 0.1740522574627903 1106 98-99 0.17539589403224198 1106 100-101 0.22573033583790902 1107 1 0.15535964834725036 1107 2 0.29907812800085765 1107 3 0.23007886182004 1107 4 0.12307424694942881 1107 5 0.10260461399592913 1107 6 0.09770390497137527 1107 7 0.09055402035613724 1107 8 0.07552134699077584 1107 9 0.054687947836541184 1107 10-11 0.10501597590000955 1107 12-13 0.07602672447080039 1107 14-15 0.14258761682529553 1107 16-17 0.15871456116283866 1107 18-19 0.19885298365277748 1107 20-21 0.1995954474046684 1107 22-23 0.18262944032794337 1107 24-25 0.19736783610257191 1107 26-27 0.2050020551812466 1107 28-29 0.2632949554545547 1107 30-31 0.21345237662499272 1107 32-33 0.21871728809944813 1107 34-35 0.2671173448645163 1107 36-37 0.2553869683854444 1107 38-39 0.26326657897821804 1107 40-41 0.2246095285672709 1107 42-43 0.22298171610809447 1107 44-45 0.2587075079676282 1107 46-47 0.25370671217124396 1107 48-49 0.2254803430926131 1107 50-51 0.28381084442643356 1107 52-53 0.2392906132238366 1107 54-55 0.25565873478357304 1107 56-57 0.22290005788200062 1107 58-59 0.21154371746072798 1107 60-61 0.20324642085550693 1107 62-63 0.16146242058525928 1107 64-65 0.15242377550294606 1107 66-67 0.18770143878610668 1107 68-69 0.1508610437888862 1107 70-71 0.1576835259993743 1107 72-73 0.1488573865234173 1107 74-75 0.14125533370140175 1107 76-77 0.11707230803860824 1107 78-79 0.12380915919257518 1107 80-81 0.1274092710435113 1107 82-83 0.07746242072503406 1107 84-85 0.10883998382599458 1107 86-87 0.12688608608276297 1107 88-89 0.16188230119049507 1107 90-91 0.11443648493621339 1107 92-93 0.12190485637535886 1107 94-95 0.10231949054363554 1107 96-97 0.1309444417268466 1107 98-99 0.1685531413085073 1107 100-101 0.1953721745573418 1108 1 0.20367529076117563 1108 2 0.30420324167720736 1108 3 0.2507102547015805 1108 4 0.14180977146300222 1108 5 0.11740896267679801 1108 6 0.1007522157372236 1108 7 0.10510712883483109 1108 8 0.1107459008247389 1108 9 0.1423304028679624 1108 10-11 0.0913348563555374 1108 12-13 0.11991226577392666 1108 14-15 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32-33 -0.8679665493363231 2214 34-35 -0.7350835981057031 2214 36-37 -0.7909548197676557 2214 38-39 -0.743531964618505 2214 40-41 -0.7242146803803422 2214 42-43 -0.7127574977830946 2214 44-45 -0.7506044120738053 2214 46-47 -0.7022322942950652 2214 48-49 -0.6434026062198086 2214 50-51 -0.6171281084211344 2214 52-53 -0.6213429319056232 2214 54-55 -0.618444984923336 2214 56-57 -0.6498442502912667 2214 58-59 -0.6033912030781678 2214 60-61 -0.5928745090901089 2214 62-63 -0.5784017415602563 2214 64-65 -0.5345424259433784 2214 66-67 -0.5874718862694692 2214 68-69 -0.47022825423002246 2214 70-71 -0.4371474228692662 2214 72-73 -0.42624606945803123 2214 74-75 -0.39352590566716117 2214 76-77 -0.4677974112109595 2214 78-79 -0.4350026501292845 2214 80-81 -0.3549757358591634 2214 82-83 -0.37406427502775585 2214 84-85 -0.3860876739240666 2214 86-87 -0.36962302209499853 2214 88-89 -0.37699311697083004 2214 90-91 -0.4008077235634815 2214 92-93 -0.3854304708353169 2214 94-95 -0.3507778173101954 2214 96-97 -0.3476166552936988 2214 98-99 -0.4128710570657361 2214 100-101 -0.4647208005259138 2215 1 -0.42094072700526297 2215 2 -0.32444851503639427 2215 3 -0.32659691024523596 2215 4 -0.2644350884364215 2215 5 -0.41468417203071795 2215 6 -0.4437765004646508 2215 7 -0.4439997461280498 2215 8 -0.4258304612452477 2215 9 -0.5095255004135453 2215 10-11 -0.4903659159515925 2215 12-13 -0.5409056491709663 2215 14-15 -0.6863248115554796 2215 16-17 -0.7293427313293037 2215 18-19 -0.6924437984384753 2215 20-21 -0.681531668370333 2215 22-23 -0.6997486110576006 2215 24-25 -0.6838879083986882 2215 26-27 -0.7480358313600348 2215 28-29 -0.7016491152711097 2215 30-31 -0.7232738978931508 2215 32-33 -0.7337269445232621 2215 34-35 -0.7677009414851952 2215 36-37 -0.7790332633029138 2215 38-39 -0.7802575627598358 2215 40-41 -0.7509991868548767 2215 42-43 -0.7186348534780365 2215 44-45 -0.7701175764491879 2215 46-47 -0.77911980906255 2215 48-49 -0.7129530502527288 2215 50-51 -0.743241267051495 2215 52-53 -0.7609938429323364 2215 54-55 -0.7023642628359781 2215 56-57 -0.7338884579045413 2215 58-59 -0.6678741782787441 2215 60-61 -0.6555864536327576 2215 62-63 -0.6480397635094803 2215 64-65 -0.6243749307377442 2215 66-67 -0.5990352028306773 2215 68-69 -0.6007281189228237 2215 70-71 -0.5769091884044286 2215 72-73 -0.5291078917320604 2215 74-75 -0.521275002798582 2215 76-77 -0.4930554901172073 2215 78-79 -0.4975889760557237 2215 80-81 -0.503826895493809 2215 82-83 -0.47308369924589755 2215 84-85 -0.4739990577168385 2215 86-87 -0.47298541194601285 2215 88-89 -0.49087238536943545 2215 90-91 -0.4665817417703906 2215 92-93 -0.46195443800444025 2215 94-95 -0.5116486810415921 2215 96-97 -0.5196622833976079 2215 98-99 -0.5389069315486523 2215 100-101 -0.6146958878544311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 19.0 13 163.0 14 665.0 15 1639.0 16 3067.0 17 5403.0 18 11515.0 19 27111.0 20 30510.0 21 27768.0 22 26968.0 23 27586.0 24 29023.0 25 33530.0 26 42120.0 27 55563.0 28 73425.0 29 99072.0 30 134292.0 31 181367.0 32 250024.0 33 345575.0 34 496326.0 35 739767.0 36 1223126.0 37 2477766.0 38 3622455.0 39 895628.0 40 1024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.79015862050348 42.56594630318066 11.855616009411035 22.788279066904828 2 21.810139901052985 28.631488881096303 32.23651207304024 17.321859144810475 3 20.67563826703229 28.69718100779572 25.528978184485908 25.09820254068608 4 21.82657968484048 28.01515562855288 26.920757368999524 23.23750731760712 5 23.085396831797176 28.09429947933712 26.127836697614427 22.692466991251276 6 23.234671874308113 26.808352295360393 26.935680270258256 23.02129556007324 7 23.512177078221136 28.40007626237756 25.809742306995844 22.278004352405464 8 22.831689098429376 26.21989654498472 27.86398415318611 23.08443020339979 9 22.537069968890215 25.87241665108554 28.147546895056102 23.442966484968146 10-11 23.121999827111605 26.697157808714184 26.87475046027622 23.30609190389799 12-13 23.229212725359055 26.965714795462816 26.532973673921628 23.27209880525651 14-15 22.941553320281088 26.930138267446562 26.769369552991446 23.358938859280908 16-17 23.154331245508057 27.026953005933535 26.673024319726057 23.14569142883235 18-19 22.754547549325434 27.23309801915747 27.033153236653924 22.979201194863172 20-21 22.267362234049457 27.053875908296977 27.373484683404804 23.30527717424876 22-23 22.311168912420612 27.00327521318989 27.802865620516975 22.88269025387252 24-25 22.237396753730426 26.71811523296803 28.592439916450164 22.452048096851378 26-27 22.177244849458674 26.57604387351382 28.88378631841531 22.362924958612194 28-29 22.280936458544208 26.67960199582067 28.78353774762143 22.2559237980137 30-31 22.328167763237538 26.64531890865997 28.68694395276814 22.33956937533435 32-33 22.262027365894347 26.680425931454632 28.806258117952417 22.251288584698603 34-35 22.325000904488 26.764283246424235 28.707956612930413 22.202759236157352 36-37 22.340918052098324 26.77625562957474 28.558534274663682 22.32429204366325 38-39 22.319311605920518 26.71742018112038 28.674147633983672 22.289120578975428 40-41 22.408462362113912 26.866750459539745 28.321963481883866 22.40282369646248 42-43 22.457171227357538 26.96548004285202 28.125190161122227 22.452158568668224 44-45 22.38470171550764 26.965802252317815 28.25552389003672 22.393972142137827 46-47 22.49233808905299 27.15301055493768 27.94736735994176 22.407283996067573 48-49 22.5159468369111 27.17043288105251 27.78128218930101 22.532338092735383 50-51 22.493465822183275 27.109079595772574 27.91156528529945 22.4858892967447 52-53 22.546312777566193 27.23709341653334 27.708785059496897 22.50780874640357 54-55 22.61215398040543 27.22263081451147 27.57823959293345 22.586975612149654 56-57 22.603730504371047 27.078027809254575 27.713029018460666 22.60521266791371 58-59 22.65021612430068 27.27580918534492 27.515740162553755 22.558234527800646 60-61 22.58622072140122 27.212499628308368 27.490423704526922 22.710855945763495 62-63 22.645327746405318 27.1460922574078 27.575988729665248 22.632591266521633 64-65 22.66344972736016 27.358460516313972 27.40231782760118 22.575771928724688 66-67 22.68786860187513 27.29557903756769 27.291915055642352 22.724637304914825 68-69 22.691270213235462 27.373157870946642 27.242198135070023 22.693373780747876 70-71 22.628333498580762 27.41861702357809 27.278018621681806 22.675030856159342 72-73 22.69556020212292 27.33630631404431 27.16944323769328 22.798690246139493 74-75 22.705820272112337 27.339463966809113 27.271385709678775 22.683330051399775 76-77 22.739739722875925 27.489834521503752 27.071758533648655 22.698667221971665 78-79 22.639500358066776 27.439150972534037 27.060108359963948 22.861240309435242 80-81 22.65781106170873 27.39334199248258 27.16732586120376 22.781521084604933 82-83 22.675440522480137 27.53160667724803 27.078985231667225 22.71396756860461 84-85 22.709069984724508 27.389190093366178 27.098810319798417 22.802929602110897 86-87 22.720084945462364 27.43995189320616 27.14249271737562 22.697470443955854 88-89 22.7264831048546 27.54115788641269 27.00343631792279 22.728922690809913 90-91 22.75927942548027 27.52312336231285 26.907799945482157 22.80979726672472 92-93 22.73346584427764 27.437631985052423 27.091362678146165 22.737539492523773 94-95 22.762676433848235 27.52741335120031 26.945691778659775 22.764218436291685 96-97 22.718846740515236 27.58765731531943 26.9020922349452 22.791403709220134 98-99 22.744540643916284 27.50604626062566 26.99816128866847 22.75125180678958 100-101 22.777148241854846 27.624798860740977 26.751965638846087 22.84608725855809 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2212.0 1 1490.5 2 579.0 3 309.0 4 212.5 5 186.5 6 157.0 7 144.5 8 147.5 9 138.0 10 147.5 11 161.0 12 208.0 13 378.0 14 555.5 15 785.0 16 2563.5 17 3578.0 18 3438.5 19 4479.5 20 6367.0 21 9316.5 22 13486.0 23 18775.0 24 24616.0 25 32570.0 26 42606.5 27 53771.5 28 65933.0 29 80297.5 30 99567.5 31 120574.0 32 144348.5 33 176020.5 34 207906.5 35 239695.0 36 275889.5 37 308647.0 38 336551.5 39 366985.0 40 395699.5 41 416271.5 42 432280.0 43 449708.5 44 466504.0 45 475273.0 46 471224.5 47 462952.5 48 455728.5 49 441332.0 50 419458.5 51 401356.5 52 390267.0 53 375902.5 54 349758.0 55 315909.5 56 281377.0 57 244663.5 58 208379.0 59 171839.0 60 134789.5 61 105277.0 62 84119.5 63 65104.5 64 50022.5 65 38569.5 66 28258.0 67 20353.0 68 14916.5 69 11351.5 70 9045.5 71 7359.5 72 6191.5 73 4954.0 74 3713.5 75 2779.0 76 1911.0 77 1330.0 78 903.5 79 486.5 80 210.5 81 124.5 82 87.5 83 40.5 84 12.0 85 5.5 86 2.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08154661280060878 2 0.002964327085323552 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0862499E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.50845883714557 #Duplication Level Percentage of deduplicated Percentage of total 1 79.17124665775623 43.9467388618756 2 12.08667436613654 13.418253330613455 3 3.6483563697719235 6.07543918124168 4 1.610746567875599 3.5764023823998485 5 0.8414718892234663 2.3354403862787954 6 0.5014731336123487 1.6701600477033274 7 0.34186923791746265 1.3283644164439463 8 0.24651151665364585 1.0946779500040982 9 0.1856819932630488 0.9276229151856978 >10 1.1600247406871447 12.55071537640649 >50 0.13023637616871697 5.070817635799781 >100 0.07477587908642881 6.839593142216294 >500 7.155823324308555E-4 0.24077210147262518 >1k 1.6591502650110417E-4 0.23788618121534827 >5k 1.6591496183328776E-5 0.08499617206143693 >10k+ 3.318299236665755E-5 0.6021199190816648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51184 0.4711991227801264 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14195 0.13067895334213608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.682393894811866E-5 0.0 0.0 0.0 0.0 2 5.523590842217799E-5 0.0 0.0 0.0 0.0 3 5.523590842217799E-5 0.0 0.0 0.0 0.0 4 1.1047181684435598E-4 0.0 0.0 0.0 0.0 5 2.0253166421465264E-4 0.0 0.0 0.0 0.0 6 4.234752979033646E-4 0.0 0.0 0.0 0.0 7 5.339471147477206E-4 0.0 0.0 0.0 0.0 8 6.812428705401952E-4 0.0 0.0 0.0 0.0 9 0.0011415421073916784 0.0 0.0 0.0 0.0 10-11 0.0024303799705758314 0.0 0.0 9.205984737029665E-6 0.0 12-13 0.0036409669634952326 0.0 0.0 9.205984737029665E-6 0.0 14-15 0.00453394748298711 0.0 0.0 1.841196947405933E-5 0.0 16-17 0.005449942964321561 0.0 0.0 2.301496184257416E-5 9.205984737029665E-6 18-19 0.006338320491444924 0.0 0.0 2.7617954211088996E-5 9.205984737029665E-6 20-21 0.007525892522521751 0.0 0.0 2.7617954211088996E-5 9.205984737029665E-6 22-23 0.009288838599662931 0.0 0.0 3.682393894811866E-5 9.205984737029665E-6 24-25 0.010932106875222728 9.205984737029665E-6 0.0 4.142693131663349E-5 9.205984737029665E-6 26-27 0.012299195608671633 9.205984737029665E-6 0.0 4.602992368514832E-5 9.205984737029665E-6 28-29 0.013855007029229646 9.205984737029665E-6 0.0 5.523590842217799E-5 9.205984737029665E-6 30-31 0.015074800006886076 9.205984737029665E-6 0.0 5.523590842217799E-5 9.205984737029665E-6 32-33 0.01666743536639221 9.205984737029665E-6 0.0 6.444189315920766E-5 9.205984737029665E-6 34-35 0.018665134054327646 9.205984737029665E-6 0.0 6.444189315920766E-5 9.205984737029665E-6 36-37 0.02110011701727199 1.841196947405933E-5 0.0 7.364787789623732E-5 9.205984737029665E-6 38-39 0.02419332788891396 2.7617954211088996E-5 0.0 8.745685500178181E-5 9.205984737029665E-6 40-41 0.027930957692148005 2.7617954211088996E-5 0.0 9.205984737029665E-5 1.841196947405933E-5 42-43 0.03246490517513512 3.222094657960383E-5 0.0 9.205984737029665E-5 1.841196947405933E-5 44-45 0.038646723926050536 4.142693131663349E-5 0.0 9.205984737029665E-5 1.841196947405933E-5 46-47 0.04600690872330575 5.0632916053663154E-5 0.0 1.0126583210732632E-4 1.841196947405933E-5 48-49 0.05510242164349106 5.523590842217799E-5 0.0 1.1967780158138564E-4 1.841196947405933E-5 50-51 0.0660069105645027 7.364787789623732E-5 0.0 1.1967780158138564E-4 2.301496184257416E-5 52-53 0.07897814305897749 7.364787789623732E-5 0.0 1.1967780158138564E-4 3.682393894811866E-5 54-55 0.09438435851639665 8.745685500178181E-5 0.0 1.3348677868693014E-4 3.682393894811866E-5 56-57 0.11219333599018053 9.205984737029665E-5 0.0 1.5189874816098946E-4 3.682393894811866E-5 58-59 0.13370772232061884 9.205984737029665E-5 0.0 1.565017405295043E-4 4.602992368514832E-5 60-61 0.1585546751258619 9.205984737029665E-5 0.0 1.565017405295043E-4 4.602992368514832E-5 62-63 0.1867848273219634 9.205984737029665E-5 0.0 1.565017405295043E-4 5.523590842217799E-5 64-65 0.22063523320002149 9.205984737029665E-5 0.0 1.7031071763504879E-4 6.904488552772249E-5 66-67 0.2599724059813492 9.205984737029665E-5 0.0 1.841196947405933E-4 8.285386263326698E-5 68-69 0.3069689580638857 9.205984737029665E-5 0.0 1.9332567947762295E-4 8.285386263326698E-5 70-71 0.3595765578436417 1.0126583210732632E-4 0.0 1.9332567947762295E-4 8.285386263326698E-5 72-73 0.4205201768027781 1.0126583210732632E-4 0.0 1.9332567947762295E-4 8.285386263326698E-5 74-75 0.4890587331699639 1.0126583210732632E-4 0.0 2.117376489516823E-4 9.205984737029665E-5 76-77 0.5677745056639361 1.0126583210732632E-4 0.0 2.2094363368871197E-4 9.205984737029665E-5 78-79 0.6574822239339216 1.0126583210732632E-4 0.0 2.2094363368871197E-4 9.205984737029665E-5 80-81 0.7598757891715341 1.1047181684435598E-4 0.0 2.3014961842574163E-4 1.0126583210732632E-4 82-83 0.874550138048344 1.1047181684435598E-4 0.0 2.4856158789980095E-4 1.0126583210732632E-4 84-85 1.0014316226864555 1.1047181684435598E-4 0.0 2.4856158789980095E-4 1.0126583210732632E-4 86-87 1.1440967681562042 1.1047181684435598E-4 0.0 2.5776757263683064E-4 1.0586882447584115E-4 88-89 1.3024581176025887 1.1967780158138564E-4 0.0 2.669735573738603E-4 1.1047181684435598E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 5360 0.0 83.81632 1 TCGACGG 5825 0.0 78.52862 2 ATCTCGA 12760 0.0 64.976654 1 CGACGGC 7210 0.0 63.706066 3 TCTCGAG 13525 0.0 59.81016 2 ACGGCGC 9100 0.0 50.579205 5 TCGAGGG 16180 0.0 50.082973 4 GACGGCG 9225 0.0 49.94534 4 CTCGAGG 16420 0.0 49.350945 3 CGAGGGC 16490 0.0 49.14145 5 GGGCGCG 16925 0.0 47.73811 8 CGGCGCG 9770 0.0 47.159237 6 AGGGCGC 17725 0.0 45.744286 7 GAGGGCG 17810 0.0 45.52597 6 TATCTCG 3180 0.0 40.629307 2 GGCGCGC 27225 0.0 30.410187 9 CGCGCCG 26760 0.0 26.128307 9 GCGCGCC 27265 0.0 25.714048 8 CTCGACG 830 0.0 22.891354 3 ATATCTC 6000 0.0 22.581625 1 >>END_MODULE