FastQCFastQC Report
Tue 31 May 2016
SRR2048245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803685
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT173350.1768212666971654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG322650.091.919651
TCGACGG328300.090.437152
CGACGGC343150.086.454223
ACGGCGC360700.082.19515
GACGGCG360900.082.149544
CGGCGCG363400.081.401416
TATCTCG70900.078.715432
CGCGCCG436300.068.279319
GGCGCGC441750.067.501447
GCGCGCC442650.067.3212668
ATCTCGA85950.065.042743
TCTCGAG84050.060.692474
AGGGCGC85350.059.6010939
TCGAGGG86500.058.589076
CGAGGGC89950.056.394717
ATATCTC101450.055.714991
CTCGAGG94050.054.1382565
GAGGGCG95550.053.0895278
CTCGACG10250.048.6556975
TCCATAT409350.036.3128718-19
Produced by FastQC (version 0.11.5)