##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9803685 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51983636765155 31.0 31.0 34.0 29.0 34.0 2 31.677929676443092 31.0 31.0 34.0 30.0 34.0 3 31.842868064406392 33.0 31.0 34.0 30.0 34.0 4 35.474401513308514 37.0 35.0 37.0 33.0 37.0 5 35.40144670090889 37.0 35.0 37.0 33.0 37.0 6 35.443370936540695 37.0 35.0 37.0 33.0 37.0 7 35.309457311204916 37.0 35.0 37.0 32.0 37.0 8 35.41628999707763 37.0 35.0 37.0 33.0 37.0 9 37.06524026424758 39.0 37.0 39.0 33.0 39.0 10-11 37.01978108231752 39.0 37.0 39.0 33.0 39.0 12-13 36.87112305219925 39.0 37.0 39.0 32.5 39.0 14-15 38.240622276215525 40.0 38.0 41.0 33.0 41.0 16-17 38.18719027590136 40.0 38.0 41.0 33.0 41.0 18-19 38.198605014338995 40.0 38.0 41.0 33.0 41.0 20-21 38.286955925246474 40.0 38.0 41.0 33.0 41.0 22-23 38.294632477481684 40.0 38.0 41.0 33.0 41.0 24-25 38.158258348773956 40.0 38.0 41.0 33.0 41.0 26-27 38.21639301956356 40.0 38.0 41.0 33.0 41.0 28-29 38.187777045060095 40.0 38.0 41.0 33.0 41.0 30-31 37.94585862356858 40.0 37.0 41.0 33.0 41.0 32-33 37.77623714960242 40.0 36.5 41.0 32.5 41.0 34-35 37.82330261529211 40.0 37.0 41.0 33.0 41.0 36-37 37.57845835520011 40.0 36.0 41.0 32.0 41.0 38-39 37.48630836262079 40.0 36.0 41.0 31.5 41.0 40-41 37.15383771510407 40.0 36.0 41.0 30.0 41.0 42-43 37.119592989778845 40.0 36.0 41.0 30.0 41.0 44-45 37.02764128998433 40.0 36.0 41.0 30.0 41.0 46-47 36.98595115000125 40.0 36.0 41.0 30.0 41.0 48-49 36.882551101958086 39.0 35.0 41.0 30.0 41.0 50-51 36.86508068139685 39.0 35.0 41.0 30.0 41.0 52-53 36.82332969694559 39.0 35.0 41.0 30.0 41.0 54-55 36.65279484193954 39.0 35.0 41.0 30.0 41.0 56-57 36.525972478715914 39.0 35.0 41.0 29.5 41.0 58-59 36.421618197647106 39.0 35.0 41.0 29.0 41.0 60-61 36.16283978932412 38.5 35.0 40.0 29.0 41.0 62-63 35.908691833733954 38.0 34.0 40.0 29.0 41.0 64-65 35.61608650216729 37.5 34.0 40.0 29.0 41.0 66-67 35.306711965959735 37.0 34.0 40.0 28.5 41.0 68-69 34.97133797138525 36.5 34.0 39.0 28.0 41.0 70-71 34.673533319358995 36.0 34.0 39.0 28.0 41.0 72-73 34.56349138104703 36.0 34.0 39.0 29.0 40.5 74-75 34.256367733153404 35.0 34.0 37.5 29.0 39.5 76-77 33.91389860037323 35.0 34.0 37.0 29.0 39.0 78-79 33.58833193844967 35.0 34.0 37.0 29.0 39.0 80-81 33.29122921636099 35.0 33.0 36.0 28.5 38.0 82-83 33.02847587412285 35.0 33.0 36.0 28.0 37.0 84-85 32.77835905580402 35.0 33.0 35.5 28.0 37.0 86-87 32.60290982421405 35.0 33.0 35.0 28.0 36.0 88-89 32.44502817052975 35.0 33.0 35.0 27.5 36.0 90-91 32.300943879775815 35.0 33.0 35.0 27.0 36.0 92-93 32.17484772307556 35.0 33.0 35.0 27.0 36.0 94-95 32.044385861030825 35.0 33.0 35.0 27.0 35.0 96-97 31.913292960759144 35.0 33.0 35.0 27.0 35.0 98-99 31.78065788527477 35.0 33.0 35.0 27.0 35.0 100-101 31.253182859302395 34.5 32.0 35.0 25.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7450370873045209 1101 2 0.7056141535848397 1101 3 0.696903460223691 1101 4 0.5913216560330596 1101 5 0.5609472469685457 1101 6 0.5558696677591684 1101 7 0.5583948386456967 1101 8 0.5570285873771184 1101 9 0.6944627499716205 1101 10-11 0.6763545476016262 1101 12-13 0.709599648812052 1101 14-15 0.9062291044058739 1101 16-17 0.9074813480651116 1101 18-19 0.8774079217024351 1101 20-21 0.8383256085256505 1101 22-23 0.8323712705811346 1101 24-25 0.8458502715974561 1101 26-27 0.8259125239889968 1101 28-29 0.8110940425470687 1101 30-31 0.8170873742068494 1101 32-33 0.8323325607233869 1101 34-35 0.77577706781247 1101 36-37 0.82185924733745 1101 38-39 0.8402836654310306 1101 40-41 0.8361611452826523 1101 42-43 0.743515022166676 1101 44-45 0.7632027471431755 1101 46-47 0.7520013859002859 1101 48-49 0.7651904771758851 1101 50-51 0.757203365219894 1101 52-53 0.7653786758440759 1101 54-55 0.7030843783647782 1101 56-57 0.7087889696843064 1101 58-59 0.6718206577504091 1101 60-61 0.6527716849552263 1101 62-63 0.5782278059407773 1101 64-65 0.5835127362815697 1101 66-67 0.5756067181914233 1101 68-69 0.5236582669191279 1101 70-71 0.5005209897857057 1101 72-73 0.44769841102819186 1101 74-75 0.4888697540158162 1101 76-77 0.47103036057684733 1101 78-79 0.46380603822260014 1101 80-81 0.4236685575314141 1101 82-83 0.4665185199308155 1101 84-85 0.47988450691132556 1101 86-87 0.4595033406716169 1101 88-89 0.4341357754417601 1101 90-91 0.4741644622270229 1101 92-93 0.5115795124381535 1101 94-95 0.5084665027270674 1101 96-97 0.524870383880323 1101 98-99 0.5461883038666926 1101 100-101 0.627058073071634 1102 1 0.6017976128608673 1102 2 0.588100433363131 1102 3 0.5612281101944028 1102 4 0.4599094047768304 1102 5 0.4542006502237541 1102 6 0.4478405743591125 1102 7 0.47084720302304106 1102 8 0.46242896030107516 1102 9 0.5649463613517227 1102 10-11 0.593141750597681 1102 12-13 0.6185454710145066 1102 14-15 0.7922796346957313 1102 16-17 0.7364679051568359 1102 18-19 0.7172776256198716 1102 20-21 0.6916056979688605 1102 22-23 0.7097040175046629 1102 24-25 0.7632859022307557 1102 26-27 0.7363404314576272 1102 28-29 0.7090188086160651 1102 30-31 0.6959163367993142 1102 32-33 0.731314499626734 1102 34-35 0.7180013083811048 1102 36-37 0.7385044007987105 1102 38-39 0.6897742356940739 1102 40-41 0.6185541275076858 1102 42-43 0.6537885164735613 1102 44-45 0.6358165652362757 1102 46-47 0.6527798353039032 1102 48-49 0.6536032057240178 1102 50-51 0.6633565329739639 1102 52-53 0.6193836283239307 1102 54-55 0.5755545513096507 1102 56-57 0.5539483661047058 1102 58-59 0.5743841079372629 1102 60-61 0.5593938337404296 1102 62-63 0.5747058287256621 1102 64-65 0.5508431537996401 1102 66-67 0.5137574660293893 1102 68-69 0.48035731552424465 1102 70-71 0.4831230084029343 1102 72-73 0.47803884479953496 1102 74-75 0.42748645331135293 1102 76-77 0.39544839375805907 1102 78-79 0.4059328645933249 1102 80-81 0.3758877701870418 1102 82-83 0.33754865839492254 1102 84-85 0.321257368081838 1102 86-87 0.3728816115764957 1102 88-89 0.3810663853280545 1102 90-91 0.4107147451150297 1102 92-93 0.40209710045728997 1102 94-95 0.4040836351926629 1102 96-97 0.4428229019842824 1102 98-99 0.4389847645092644 1102 100-101 0.4635046631464199 1103 1 0.5066897571595916 1103 2 0.433564443981183 1103 3 0.4263980474860425 1103 4 0.4286681216442929 1103 5 0.3494289555392456 1103 6 0.39197902692873754 1103 7 0.37586358530467123 1103 8 0.35385006064603886 1103 9 0.42458217364553263 1103 10-11 0.4879962108830327 1103 12-13 0.5126167403641446 1103 14-15 0.6504753842908357 1103 16-17 0.6294009823570406 1103 18-19 0.5806305937222902 1103 20-21 0.5682855725497262 1103 22-23 0.5958872532372297 1103 24-25 0.5766730772514563 1103 26-27 0.6375439458211005 1103 28-29 0.6022522482569741 1103 30-31 0.6086275262891689 1103 32-33 0.635286056759675 1103 34-35 0.5780031386216535 1103 36-37 0.5693775063730726 1103 38-39 0.6115548808822169 1103 40-41 0.6196798626804494 1103 42-43 0.5419755081806557 1103 44-45 0.5521633671050736 1103 46-47 0.5661204188188549 1103 48-49 0.5742993097246369 1103 50-51 0.5379813457272036 1103 52-53 0.4908995959360283 1103 54-55 0.5112560036664462 1103 56-57 0.46413836003365816 1103 58-59 0.44524744911195313 1103 60-61 0.45109597011494884 1103 62-63 0.4726147065143351 1103 64-65 0.4317971663818483 1103 66-67 0.46269126645812264 1103 68-69 0.43860926629282204 1103 70-71 0.43914765626562513 1103 72-73 0.40313805270472614 1103 74-75 0.3606759698937907 1103 76-77 0.3745237570191833 1103 78-79 0.4033782945900555 1103 80-81 0.35290418070047025 1103 82-83 0.3267936199248851 1103 84-85 0.3176301332459275 1103 86-87 0.35844423952442384 1103 88-89 0.3273509291577881 1103 90-91 0.3565759025485278 1103 92-93 0.34194951604905555 1103 94-95 0.31895650224457484 1103 96-97 0.37446385499788803 1103 98-99 0.3708133262273421 1103 100-101 0.4386632347287325 1104 1 0.3595692422445005 1104 2 0.3591928412422334 1104 3 0.3099127417875387 1104 4 0.3049527417183313 1104 5 0.33856364082701873 1104 6 0.32121857891144856 1104 7 0.31066738160320284 1104 8 0.29386104811424474 1104 9 0.37089156577015103 1104 10-11 0.3870083914700473 1104 12-13 0.42756913059017876 1104 14-15 0.5353341477949485 1104 16-17 0.49641792565358855 1104 18-19 0.4567319424921692 1104 20-21 0.4655369838067145 1104 22-23 0.4373509489441787 1104 24-25 0.45242939709139307 1104 26-27 0.4668678196265077 1104 28-29 0.4837259205169602 1104 30-31 0.47846893594869755 1104 32-33 0.49454935166041025 1104 34-35 0.46572530813459423 1104 36-37 0.440104418735622 1104 38-39 0.48254053601093716 1104 40-41 0.44019453661432095 1104 42-43 0.4263021711861228 1104 44-45 0.3947737422424069 1104 46-47 0.43505997866489565 1104 48-49 0.46034334512953734 1104 50-51 0.42860636859225565 1104 52-53 0.4300650282757772 1104 54-55 0.4325221003999502 1104 56-57 0.42772603942369614 1104 58-59 0.4539746147856576 1104 60-61 0.4116494406434583 1104 62-63 0.3931587081872294 1104 64-65 0.38156512402053266 1104 66-67 0.4233731620366541 1104 68-69 0.3895463055107484 1104 70-71 0.35812270490826137 1104 72-73 0.3221690936537769 1104 74-75 0.32203112452035754 1104 76-77 0.32387163610273717 1104 78-79 0.2995199651373781 1104 80-81 0.2828831006554253 1104 82-83 0.30978682012421643 1104 84-85 0.3142747087035147 1104 86-87 0.3065481703239499 1104 88-89 0.31111951398197846 1104 90-91 0.3108189442044136 1104 92-93 0.3296099786731048 1104 94-95 0.3585438244246717 1104 96-97 0.3569972978485261 1104 98-99 0.39995321347252144 1104 100-101 0.3800874129107008 1105 1 0.36478168894878493 1105 2 0.33337318071506417 1105 3 0.3077795560561931 1105 4 0.3066892332070026 1105 5 0.28333856137256674 1105 6 0.29237947462841873 1105 7 0.26380753031527604 1105 8 0.27916359906981825 1105 9 0.36575509921139826 1105 10-11 0.36005991836231743 1105 12-13 0.36243211590053903 1105 14-15 0.4901636359800179 1105 16-17 0.4924597911563069 1105 18-19 0.4388875771777805 1105 20-21 0.4057897765804199 1105 22-23 0.42199442634553463 1105 24-25 0.4420171725718802 1105 26-27 0.44686649251843136 1105 28-29 0.4707377094538572 1105 30-31 0.43802214230677805 1105 32-33 0.4349982605329359 1105 34-35 0.4198803360853205 1105 36-37 0.4015794942719637 1105 38-39 0.4113270346267086 1105 40-41 0.4125057291055896 1105 42-43 0.37978545669358255 1105 44-45 0.4378007466201481 1105 46-47 0.4341676526278846 1105 48-49 0.42756413706760554 1105 50-51 0.3826651989082279 1105 52-53 0.42940343790689894 1105 54-55 0.38015352814822734 1105 56-57 0.39603958988082866 1105 58-59 0.4029593512948608 1105 60-61 0.41295092330432226 1105 62-63 0.399781413728995 1105 64-65 0.43156202898383356 1105 66-67 0.38384264565701187 1105 68-69 0.38330875374060724 1105 70-71 0.427929388023621 1105 72-73 0.3702996210535616 1105 74-75 0.31098997475179857 1105 76-77 0.35557779239661613 1105 78-79 0.3533758273496659 1105 80-81 0.3378375146700634 1105 82-83 0.3124179110803027 1105 84-85 0.32662356402634174 1105 86-87 0.31038771398934983 1105 88-89 0.37876826729802104 1105 90-91 0.35893278134314954 1105 92-93 0.39125065471407794 1105 94-95 0.36502453183625505 1105 96-97 0.39954440006261294 1105 98-99 0.4726309736583758 1105 100-101 0.496397413320544 1106 1 0.2197024661574858 1106 2 0.2711862357022419 1106 3 0.23603173354139173 1106 4 0.23821189863097203 1106 5 0.2473088585667682 1106 6 0.18401477169091152 1106 7 0.19590846482230262 1106 8 0.18420914087974438 1106 9 0.2561447910394534 1106 10-11 0.24102273523457995 1106 12-13 0.2823465634278577 1106 14-15 0.33819845817455274 1106 16-17 0.3682341306565746 1106 18-19 0.3575799770250896 1106 20-21 0.31240353824892964 1106 22-23 0.302888333625674 1106 24-25 0.3187991812564732 1106 26-27 0.3075873756816847 1106 28-29 0.3126184009971027 1106 30-31 0.3028540671947013 1106 32-33 0.2956566213547447 1106 34-35 0.29855055382951434 1106 36-37 0.3171553049394973 1106 38-39 0.3282069022767402 1106 40-41 0.30304828925517313 1106 42-43 0.2865241004404879 1106 44-45 0.27595609612547634 1106 46-47 0.2725238680127475 1106 48-49 0.2589815217454756 1106 50-51 0.2366006970233201 1106 52-53 0.2698452002614715 1106 54-55 0.23319908090563501 1106 56-57 0.22190012492715283 1106 58-59 0.27485985979492256 1106 60-61 0.240375158169698 1106 62-63 0.26920261112049815 1106 64-65 0.23060817129032074 1106 66-67 0.22721594962317226 1106 68-69 0.2034994820155518 1106 70-71 0.24652848687389906 1106 72-73 0.1793465536178971 1106 74-75 0.18703893862628007 1106 76-77 0.21525074075259454 1106 78-79 0.16044384495200603 1106 80-81 0.20189761083552327 1106 82-83 0.23193979847282264 1106 84-85 0.18395733528829084 1106 86-87 0.1621468751895705 1106 88-89 0.2575532052532452 1106 90-91 0.20798063501626274 1106 92-93 0.2267654771496339 1106 94-95 0.27277321988930225 1106 96-97 0.2690326270459771 1106 98-99 0.27274181730518876 1106 100-101 0.28271434234882875 1107 1 0.2338050595960368 1107 2 0.22250007883475575 1107 3 0.2445661020311185 1107 4 0.20286424706510076 1107 5 0.21218234489688825 1107 6 0.15172536842554507 1107 7 0.15816738660061702 1107 8 0.158727053171269 1107 9 0.25423340663839866 1107 10-11 0.2372288683472874 1107 12-13 0.2838045525046837 1107 14-15 0.31145718044428605 1107 16-17 0.34326774867210474 1107 18-19 0.2760410900807386 1107 20-21 0.25458540177965006 1107 22-23 0.25793794602959963 1107 24-25 0.21382993768310854 1107 26-27 0.20695923408123207 1107 28-29 0.2627188625896153 1107 30-31 0.2339954436105387 1107 32-33 0.19956210595150026 1107 34-35 0.17710004044857897 1107 36-37 0.20631396166275096 1107 38-39 0.1451815633571698 1107 40-41 0.1321000594470192 1107 42-43 0.12589652601378987 1107 44-45 0.14047888039072376 1107 46-47 0.1063361837893666 1107 48-49 0.08944888710456667 1107 50-51 0.20447903461800365 1107 52-53 0.11969971952983371 1107 54-55 0.1384563924249136 1107 56-57 0.18394076906801615 1107 58-59 0.21682275279611218 1107 60-61 0.2246572392618873 1107 62-63 0.25537300053560585 1107 64-65 0.23661655455290997 1107 66-67 0.2171445746427878 1107 68-69 0.1571653722883184 1107 70-71 0.16408661741601094 1107 72-73 0.19642995773708805 1107 74-75 0.16706920659171232 1107 76-77 0.17720110382582277 1107 78-79 0.1247857222988813 1107 80-81 0.1711506297053873 1107 82-83 0.12643303693248953 1107 84-85 0.11093833261742958 1107 86-87 0.1451828480626105 1107 88-89 0.1465757890564916 1107 90-91 0.13941603603271346 1107 92-93 0.11295516333225919 1107 94-95 0.11378135858869598 1107 96-97 0.14961701578144826 1107 98-99 0.13544399157617448 1107 100-101 0.2026162552143589 1108 1 0.1921403536683144 1108 2 0.21529925259968152 1108 3 0.22962071729319078 1108 4 0.20212687122242556 1108 5 0.2131227444282473 1108 6 0.13691659141743173 1108 7 0.1856837146302297 1108 8 0.15945128075473747 1108 9 0.19569463501332507 1108 10-11 0.2156912186098907 1108 12-13 0.21903148586190468 1108 14-15 0.26854157290248537 1108 16-17 0.2565213361650507 1108 18-19 0.2681800142348152 1108 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-0.7622609832270015 2215 12-13 -0.6884768649291217 2215 14-15 -0.8077585501714069 2215 16-17 -0.8539693780775366 2215 18-19 -0.8904938509343765 2215 20-21 -0.8724048820422468 2215 22-23 -0.8513957511380994 2215 24-25 -0.823218386060141 2215 26-27 -0.9202182709030851 2215 28-29 -0.9307167634777755 2215 30-31 -0.8593473803920801 2215 32-33 -0.8653979606347519 2215 34-35 -0.8718882484778518 2215 36-37 -0.8178910301820181 2215 38-39 -0.8644648168411777 2215 40-41 -0.8386974551683366 2215 42-43 -0.8546482607629713 2215 44-45 -0.8028811112226393 2215 46-47 -0.7749178496208131 2215 48-49 -0.8097094676028647 2215 50-51 -0.8189101347538355 2215 52-53 -0.7562537637641071 2215 54-55 -0.748326640881956 2215 56-57 -0.7612268337261554 2215 58-59 -0.8113724067156056 2215 60-61 -0.7750496750097113 2215 62-63 -0.7324390274369605 2215 64-65 -0.768838854371154 2215 66-67 -0.7257469232169313 2215 68-69 -0.6941659530838962 2215 70-71 -0.6387507363920264 2215 72-73 -0.5746239206935755 2215 74-75 -0.605929361961401 2215 76-77 -0.600647020864983 2215 78-79 -0.5510151760534114 2215 80-81 -0.5906654148995045 2215 82-83 -0.6091770490952371 2215 84-85 -0.5948656875274807 2215 86-87 -0.5401402949102518 2215 88-89 -0.5934496290906921 2215 90-91 -0.5483223914993474 2215 92-93 -0.5308802951191645 2215 94-95 -0.5875683080153422 2215 96-97 -0.6457914862499479 2215 98-99 -0.625613615695201 2215 100-101 -0.6744000376285477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 109.0 11 1308.0 12 5200.0 13 10467.0 14 15205.0 15 17817.0 16 19988.0 17 23184.0 18 30774.0 19 44253.0 20 44264.0 21 46183.0 22 55906.0 23 66018.0 24 74009.0 25 84397.0 26 97572.0 27 116147.0 28 139261.0 29 165144.0 30 191458.0 31 228760.0 32 283023.0 33 360871.0 34 475950.0 35 672827.0 36 1105639.0 37 2099652.0 38 2697359.0 39 630155.0 40 783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.754615788632414 39.71871799284151 12.39604829758766 21.13061792093841 2 23.66696077481346 28.105154712785545 31.39565365960637 16.832230852794623 3 22.60437456049676 27.685863331756376 23.33077511082061 26.37898699692626 4 25.262164176021567 26.649244646273313 24.93868377043938 23.149907407265736 5 23.034685426959353 29.62419743188403 24.779692533980846 22.56142460717577 6 22.39278393787642 25.44569720467355 26.137967509155995 26.02355134829403 7 26.051418420726492 26.281658376416623 24.578390676567025 23.08853252628986 8 25.472850259876772 25.228697168462677 26.346072930739815 22.952379640920736 9 22.09921065395308 25.455571042929265 26.443617884499552 26.001600418618104 10-11 24.56831283338867 25.750888568941164 25.563367244051598 24.117431353618564 12-13 24.38532041778168 25.946065178552757 25.30479100460694 24.36382339905862 14-15 24.16454735248719 25.911849960461286 25.513984158503707 24.40961852854782 16-17 24.313219977998067 27.319813927110058 25.426362638130456 22.94060345676141 18-19 22.60738933378283 25.7150937328904 27.104936006420143 24.572580926906628 20-21 22.56019640832024 27.62116446738554 25.617830609758112 24.200808514536103 22-23 22.37549678753623 27.439905443019093 27.51201151616305 22.672586253281636 24-25 22.286163067652303 25.759700732770792 29.253450196938815 22.70068600263809 26-27 22.196455181993826 25.673215424345564 29.75101487366115 22.379314519999465 28-29 22.251699233502503 25.6897074926418 29.763446091954197 22.2951471819015 30-31 22.337014688103167 25.653997872738127 29.663659548338085 22.345327890820617 32-33 22.28173836833639 25.690778449600455 29.756674477236437 22.270808704826713 34-35 22.281063103776884 25.805270025635853 29.693300532633117 22.220366337954147 36-37 22.347214338281983 25.797462892779606 29.548343301523865 22.306979467414546 38-39 22.421176322984675 25.805990298545904 29.365784396377485 22.407048982091936 40-41 22.871867163062166 26.316003695219475 27.835206717660917 22.976922424057445 42-43 22.873658512825205 26.452700264529554 27.88259304756253 22.79104817508271 44-45 22.870908474810015 26.70897621673958 27.738795004048505 22.681320304401904 46-47 22.8515003721305 27.001506423426825 27.540523157390467 22.606470047052206 48-49 22.82221690335756 27.04393794187172 27.391042312225732 22.742802842544986 50-51 22.807891812859236 27.03454488870964 27.39179342775299 22.76576987067814 52-53 22.856127783414568 27.150716605476877 27.229478079136026 22.76367753197253 54-55 22.977555020257945 27.018336830402895 27.141734314277453 22.862373835061707 56-57 22.993838785332123 26.90285038736779 27.276511570797357 22.826799256502735 58-59 22.979857107761 27.026928608400837 27.171435546942234 22.821778736895933 60-61 22.95960443827632 26.9794255753 27.12741538673732 22.933554599686364 62-63 23.014703853449163 26.951595342265776 27.154259202767754 22.879441601517303 64-65 23.052615295209076 27.149761425651004 26.998865719244623 22.798757559895293 66-67 23.121788073860014 27.060127252240363 26.888980929912528 22.929103743987095 68-69 23.096930311807874 27.1424059952044 26.84541211471047 22.915251578277257 70-71 23.06540189715427 27.206059844751213 26.8670036114487 22.861534646645826 72-73 23.113829955212715 27.134944076079215 26.78316276466093 22.968063204047134 74-75 23.165241980126435 27.124154539159136 26.86714382776993 22.843459652944503 76-77 23.217275491179507 27.26105165363988 26.665798622267673 22.85587423291294 78-79 23.163018267572593 27.188275712899134 26.652925912999947 22.995780106528326 80-81 23.140358880817367 27.141900139973057 26.75413728885782 22.963603690351754 82-83 23.202988156639027 27.297300715775247 26.66944182750155 22.830269300084172 84-85 23.24869885811681 27.16500139378456 26.690803495759607 22.895496252339022 86-87 23.296698308440607 27.20610778128541 26.699412668219384 22.797781242054597 88-89 23.322934596183337 27.287014937826886 26.55150225931036 22.838548206679416 90-91 23.382571768621855 27.254592910269178 26.4877359187269 22.875099402382066 92-93 23.351426397751577 27.17880135535034 26.66051556537864 22.809256681519447 94-95 23.408813553513045 27.28714818636529 26.536788217586388 22.767250042535274 96-97 23.453033404837168 27.28298178131251 26.51091377004734 22.753071043802983 98-99 23.485860898275813 27.256281224558343 26.54610413445194 22.711753742713896 100-101 23.559369569697825 27.356382971398617 26.38296476832827 22.701282690575283 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 943.0 1 806.0 2 575.5 3 446.5 4 387.0 5 330.5 6 270.0 7 247.0 8 233.0 9 202.0 10 186.5 11 185.5 12 196.0 13 224.5 14 250.5 15 318.0 16 1101.5 17 1573.5 18 1630.5 19 2377.5 20 3709.0 21 5804.0 22 8633.0 23 12339.0 24 17144.5 25 23782.5 26 32136.5 27 42055.0 28 53186.5 29 66067.0 30 82736.5 31 101085.5 32 120788.0 33 145319.5 34 170082.5 35 196347.5 36 226372.5 37 253047.0 38 276953.0 39 301674.5 40 325705.0 41 345758.5 42 363327.5 43 382434.0 44 402780.5 45 416955.0 46 418897.5 47 417015.0 48 414753.5 49 407848.5 50 395330.5 51 383808.0 52 376589.5 53 364942.0 54 342932.5 55 313858.0 56 282792.5 57 248740.5 58 215168.0 59 180851.5 60 143444.0 61 113371.5 62 91664.0 63 71881.0 64 55706.0 65 42949.5 66 31696.0 67 22757.5 68 16855.0 69 12932.0 70 10470.0 71 8973.5 72 7734.5 73 6112.5 74 4593.0 75 3613.0 76 2606.0 77 1808.0 78 1250.5 79 747.0 80 346.5 81 165.0 82 93.5 83 41.0 84 19.5 85 9.5 86 7.5 87 4.0 88 3.0 89 5.0 90 4.0 91 2.0 92 3.0 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 2.0 99 3.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002182852672234981 2 3.570086146178707E-4 3 3.060073839581749E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.550061532984791E-5 16-17 0.0 18-19 0.001657539996440114 20-21 2.7540664556235744E-4 22-23 7.395178445655893E-4 24-25 5.61013537256654E-5 26-27 0.0025296610407209126 28-29 0.0 30-31 2.550061532984791E-5 32-33 9.129220288085552E-4 34-35 3.315079992880228E-4 36-37 0.0 38-39 0.0 40-41 0.007639984352822433 42-43 0.004411606452063688 44-45 6.83416490839924E-4 46-47 5.1001230659695815E-6 48-49 5.61013537256654E-5 50-51 6.120147679163498E-5 52-53 3.468083684859316E-4 54-55 5.1001230659695815E-6 56-57 1.7850430730893536E-4 58-59 3.570086146178707E-5 60-61 0.013474525140291635 62-63 0.005166424665827186 64-65 0.005676436972424145 66-67 8.721210442807985E-4 68-69 0.0011832285513049428 70-71 1.5300369197908745E-5 72-73 9.180221518745246E-5 74-75 8.16019690555133E-5 76-77 3.978095991456274E-4 78-79 1.5300369197908745E-5 80-81 1.2750307664923953E-4 82-83 4.99812060465019E-4 84-85 0.012622804588274715 86-87 0.004615611374702472 88-89 0.00256026177911673 90-91 9.894238747980989E-4 92-93 2.0910504570475287E-4 94-95 5.814140295205323E-4 96-97 1.5300369197908747E-4 98-99 3.570086146178707E-5 100-101 0.0019431468881344108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9803685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.73432146583815 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57527069062243 48.13109121630567 2 11.421254545770084 13.64481781160391 3 3.343858756585076 5.992294017066324 4 1.4566815383183962 3.4805553313305087 5 0.7759632978713799 2.317582054037046 6 0.47005845504719473 1.6847173716914619 7 0.31457798292768835 1.3153771650794237 8 0.23212063016644713 1.1092454672972385 9 0.17305174385881403 0.930341564809765 >10 1.0847176034091484 12.355210330808077 >50 0.10963031390269865 4.524909550410533 >100 0.04235115676016933 4.00975310796178 >500 2.747635090801734E-4 0.10330967605269499 >1k 1.5424473698377755E-4 0.1323906157079808 >5k 1.7138257138144295E-5 0.09093901618420228 >10k+ 1.7138257138144295E-5 0.17746570365339936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 17335 0.1768212666971654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0200246131939163E-5 0.0 0.0 0.0 0.0 2 6.120147679163498E-5 0.0 0.0 0.0 0.0 3 8.16019690555133E-5 0.0 0.0 0.0 0.0 4 1.122027074513308E-4 0.0 0.0 0.0 0.0 5 1.8360443037490496E-4 0.0 0.0 1.0200246131939163E-5 0.0 6 4.794115682011407E-4 1.0200246131939163E-5 0.0 2.0400492263878326E-5 0.0 7 6.936167369718632E-4 1.0200246131939163E-5 0.0 3.060073839581749E-5 0.0 8 9.282223980064639E-4 3.060073839581749E-5 0.0 3.060073839581749E-5 0.0 9 0.0019074460266726237 3.060073839581749E-5 0.0 4.080098452775665E-5 0.0 10-11 0.004396306082865779 3.060073839581749E-5 0.0 4.080098452775665E-5 0.0 12-13 0.008445803797245627 3.060073839581749E-5 0.0 6.120147679163498E-5 0.0 14-15 0.010174745516609317 3.060073839581749E-5 0.0 6.630159985760456E-5 0.0 16-17 0.011786384405455704 3.060073839581749E-5 0.0 8.670209212148288E-5 0.0 18-19 0.01341842378656597 4.080098452775665E-5 0.0 9.180221518745248E-5 0.0 20-21 0.01499946193701654 4.080098452775665E-5 5.1001230659695815E-6 1.0200246131939164E-4 0.0 22-23 0.02199173066046084 4.080098452775665E-5 1.0200246131939163E-5 1.0200246131939164E-4 0.0 24-25 0.02926450615253346 4.080098452775665E-5 2.0400492263878326E-5 1.122027074513308E-4 0.0 26-27 0.033594510635541634 6.120147679163498E-5 3.060073839581749E-5 1.2240295358326996E-4 0.0 28-29 0.03765930871911939 6.120147679163498E-5 5.61013537256654E-5 1.3260319971520912E-4 0.0 30-31 0.03949025289980247 6.120147679163498E-5 7.140172292357414E-5 1.3260319971520912E-4 0.0 32-33 0.04164250483364164 6.120147679163498E-5 9.690233825342206E-5 1.3260319971520912E-4 0.0 34-35 0.043861058367338404 6.120147679163498E-5 1.122027074513308E-4 1.3260319971520912E-4 0.0 36-37 0.04740054377512129 6.120147679163498E-5 1.3260319971520912E-4 1.4790356891311785E-4 0.0 38-39 0.05185805133477871 6.120147679163498E-5 1.4280344584714828E-4 1.5300369197908745E-4 0.0 40-41 0.05649406320174506 6.630159985760456E-5 1.5300369197908745E-4 1.5810381504505704E-4 0.0 42-43 0.06294571888019658 7.140172292357414E-5 1.5300369197908745E-4 1.734041842429658E-4 0.0 44-45 0.07085090963244943 7.140172292357414E-5 1.5300369197908745E-4 1.734041842429658E-4 5.1001230659695815E-6 46-47 0.08106135601052053 7.140172292357414E-5 1.5300369197908745E-4 1.8870455344087455E-4 1.0200246131939163E-5 48-49 0.09483678841170437 8.670209212148288E-5 1.5300369197908745E-4 1.9380467650684412E-4 1.0200246131939163E-5 50-51 0.11139178788384163 9.180221518745248E-5 1.5300369197908745E-4 2.0910504570475287E-4 1.0200246131939163E-5 52-53 0.13176677953239013 9.180221518745248E-5 1.5300369197908745E-4 2.1420516877072244E-4 1.0200246131939163E-5 54-55 0.15568635671178746 1.0710258438536122E-4 1.5300369197908745E-4 2.1420516877072244E-4 1.0200246131939163E-5 56-57 0.18408894206617205 1.122027074513308E-4 1.5300369197908745E-4 2.1420516877072244E-4 1.0200246131939163E-5 58-59 0.2176477518402519 1.122027074513308E-4 1.5300369197908745E-4 2.244054149026616E-4 2.0400492263878326E-5 60-61 0.25778062024636655 1.122027074513308E-4 1.5300369197908745E-4 2.244054149026616E-4 2.0400492263878326E-5 62-63 0.30351342377891577 1.2240295358326996E-4 1.5300369197908745E-4 2.3460566103460076E-4 3.060073839581749E-5 64-65 0.3567740089568361 1.2240295358326996E-4 1.5300369197908745E-4 2.448059071665399E-4 4.080098452775665E-5 66-67 0.4177102793490407 1.2240295358326996E-4 1.5300369197908745E-4 2.448059071665399E-4 4.080098452775665E-5 68-69 0.48681694689292854 1.2240295358326996E-4 1.5300369197908745E-4 2.499060302325095E-4 4.080098452775665E-5 70-71 0.563201490051955 1.2240295358326996E-4 1.5300369197908745E-4 2.6520639943041825E-4 4.080098452775665E-5 72-73 0.6505512978028161 1.2750307664923953E-4 1.5300369197908745E-4 2.6520639943041825E-4 4.080098452775665E-5 74-75 0.74812175217788 1.3260319971520912E-4 1.5300369197908745E-4 2.6520639943041825E-4 4.590110759372624E-5 76-77 0.8570297801285944 1.3260319971520912E-4 1.5300369197908745E-4 2.7540664556235744E-4 5.100123065969582E-5 78-79 0.975811646335026 1.3770332278117872E-4 1.5300369197908745E-4 2.8050676862832703E-4 5.100123065969582E-5 80-81 1.1077110290671315 1.4280344584714828E-4 1.5300369197908745E-4 2.9070701476026616E-4 5.100123065969582E-5 82-83 1.2530186353396706 1.4280344584714828E-4 1.5300369197908745E-4 3.009072608922053E-4 5.100123065969582E-5 84-85 1.4100208238024785 1.4280344584714828E-4 1.5300369197908745E-4 3.111075070241445E-4 5.100123065969582E-5 86-87 1.5803088328521366 1.4280344584714828E-4 1.5300369197908745E-4 3.213077531560837E-4 5.100123065969582E-5 88-89 1.7661573173760683 1.4280344584714828E-4 1.5300369197908745E-4 3.264078762220532E-4 6.120147679163498E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 32265 0.0 91.91965 1 TCGACGG 32830 0.0 90.43715 2 CGACGGC 34315 0.0 86.45422 3 ACGGCGC 36070 0.0 82.1951 5 GACGGCG 36090 0.0 82.14954 4 CGGCGCG 36340 0.0 81.40141 6 TATCTCG 7090 0.0 78.71543 2 CGCGCCG 43630 0.0 68.27931 9 GGCGCGC 44175 0.0 67.50144 7 GCGCGCC 44265 0.0 67.321266 8 ATCTCGA 8595 0.0 65.04274 3 TCTCGAG 8405 0.0 60.69247 4 AGGGCGC 8535 0.0 59.601093 9 TCGAGGG 8650 0.0 58.58907 6 CGAGGGC 8995 0.0 56.39471 7 ATATCTC 10145 0.0 55.71499 1 CTCGAGG 9405 0.0 54.138256 5 GAGGGCG 9555 0.0 53.089527 8 CTCGACG 1025 0.0 48.655697 5 TCCATAT 40935 0.0 36.31287 18-19 >>END_MODULE