FastQCFastQC Report
Tue 31 May 2016
SRR2048245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803685
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT503980.51407200455747No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142970.14583291894833422No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT100030.10203306205778745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG65400.084.609161
TCGACGG69950.079.3822252
ATCTCGA130000.063.5913931
CGACGGC89050.062.8349383
TCTCGAG144050.056.4859662
GACGGCG112700.049.5647854
ACGGCGC112650.049.502455
TCGAGGG167450.048.7335244
CTCGAGG171050.047.7356263
CGAGGGC176850.046.2775155
CGGCGCG121150.045.990116
GGGCGCG182650.044.7559748
AGGGCGC186850.043.902487
GAGGGCG187500.043.623626
TATCTCG49000.031.4083672
ATATCTC59050.026.9691351
GGCGCGC312250.026.6514919
CATATGT75550.025.966024
ACATATG77700.025.6143193
CGCGCCG304600.025.57439
Produced by FastQC (version 0.11.5)