##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048245_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9803685 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35450547421709 33.0 31.0 34.0 31.0 34.0 2 32.45360831156856 34.0 31.0 34.0 31.0 34.0 3 32.600949642914884 34.0 31.0 34.0 31.0 34.0 4 36.216589578306525 37.0 35.0 37.0 35.0 37.0 5 36.072694910128185 37.0 35.0 37.0 35.0 37.0 6 36.02839401714763 37.0 35.0 37.0 35.0 37.0 7 35.97886029589894 37.0 35.0 37.0 35.0 37.0 8 35.961117681769665 37.0 35.0 37.0 35.0 37.0 9 37.641791836437015 39.0 37.0 39.0 35.0 39.0 10-11 37.68373106643063 39.0 37.0 39.0 35.0 39.0 12-13 37.69571349956674 39.0 37.0 39.0 35.0 39.0 14-15 39.09733217662542 40.0 38.0 41.0 36.0 41.0 16-17 39.0989967547917 40.0 38.0 41.0 36.0 41.0 18-19 39.13591909572778 40.0 38.5 41.0 36.0 41.0 20-21 39.1664601116825 40.0 39.0 41.0 36.0 41.0 22-23 39.15410363552073 40.0 39.0 41.0 36.0 41.0 24-25 39.117558193679216 40.0 39.0 41.0 36.0 41.0 26-27 39.02823530131782 40.0 38.0 41.0 35.5 41.0 28-29 38.91906900313505 40.0 38.0 41.0 35.0 41.0 30-31 38.802921146487265 40.0 38.0 41.0 35.0 41.0 32-33 38.71519571467259 40.0 38.0 41.0 35.0 41.0 34-35 38.645561031387686 40.0 38.0 41.0 35.0 41.0 36-37 38.58722138665206 40.0 38.0 41.0 35.0 41.0 38-39 38.539562521643646 40.0 38.0 41.0 35.0 41.0 40-41 38.575649003410454 40.0 38.0 41.0 35.0 41.0 42-43 38.53441996555377 40.0 38.0 41.0 34.0 41.0 44-45 38.45701978388739 40.0 38.0 41.0 34.0 41.0 46-47 38.384596863322315 40.0 38.0 41.0 34.0 41.0 48-49 38.28219144127948 40.0 38.0 41.0 34.0 41.0 50-51 38.17120144109077 40.0 37.0 41.0 33.5 41.0 52-53 38.050325617357146 40.0 37.0 41.0 33.0 41.0 54-55 37.86944903880531 40.0 37.0 41.0 33.0 41.0 56-57 37.65322957642968 40.0 36.5 41.0 33.0 41.0 58-59 37.44464678332688 39.0 36.0 41.0 33.0 41.0 60-61 37.190728537279604 39.0 36.0 41.0 32.0 41.0 62-63 37.035082573542496 39.0 35.0 41.0 32.0 41.0 64-65 36.808021779565536 38.5 35.0 40.0 32.0 41.0 66-67 36.51146201657846 38.0 35.0 40.0 32.0 41.0 68-69 36.169732401642854 37.0 35.0 40.0 32.0 41.0 70-71 35.801438387708295 37.0 35.0 39.0 31.0 41.0 72-73 35.429153731479545 36.0 35.0 39.0 31.0 40.5 74-75 35.05517292732274 36.0 34.5 38.5 31.0 40.0 76-77 34.689827957548616 35.0 34.0 37.0 31.0 39.0 78-79 34.34778106395707 35.0 34.0 37.0 31.0 39.0 80-81 34.0576101741335 35.0 34.0 36.0 31.0 38.0 82-83 33.7956380687466 35.0 34.0 36.0 30.5 37.0 84-85 33.56514458593886 35.0 34.0 36.0 30.0 37.0 86-87 33.37682279673409 35.0 34.0 35.0 30.0 36.5 88-89 33.230868953867855 35.0 34.0 35.0 30.0 36.0 90-91 33.09498749704831 35.0 34.0 35.0 30.0 36.0 92-93 32.95543058554003 35.0 34.0 35.0 30.0 36.0 94-95 32.84972528187105 35.0 34.0 35.0 30.0 35.0 96-97 32.733811316867076 35.0 34.0 35.0 30.0 35.0 98-99 32.64160598795249 35.0 34.0 35.0 30.0 35.0 100-101 32.04561279763681 34.5 33.0 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49484443965863534 1101 2 0.5942351325633126 1101 3 0.5199267465059165 1101 4 0.3133000912323354 1101 5 0.34641777095421844 1101 6 0.3699312209076595 1101 7 0.36504355248793274 1101 8 0.36072339480260496 1101 9 0.42934143244340817 1101 10-11 0.3929197495849692 1101 12-13 0.43754436061087176 1101 14-15 0.5515465781852313 1101 16-17 0.5510663921110108 1101 18-19 0.5075565691325039 1101 20-21 0.5081778521515901 1101 22-23 0.5353728864320644 1101 24-25 0.5518487816993556 1101 26-27 0.5426421033537494 1101 28-29 0.5891657312275882 1101 30-31 0.6067351700384762 1101 32-33 0.5970977092839362 1101 34-35 0.6193248457556564 1101 36-37 0.6120737174531499 1101 38-39 0.5927897152719481 1101 40-41 0.5723574474915054 1101 42-43 0.6194538231375262 1101 44-45 0.6138096451389785 1101 46-47 0.6264840805947998 1101 48-49 0.6279654476704764 1101 50-51 0.6140508178740376 1101 52-53 0.6122763731162166 1101 54-55 0.5781351541576356 1101 56-57 0.5741861168514646 1101 58-59 0.5704393105372532 1101 60-61 0.5360150883761676 1101 62-63 0.5423594406351526 1101 64-65 0.4991257811536727 1101 66-67 0.4613993935972758 1101 68-69 0.4687696753480992 1101 70-71 0.4913107095519891 1101 72-73 0.46181419416821257 1101 74-75 0.4108893263509401 1101 76-77 0.39969939603714266 1101 78-79 0.41546394062208236 1101 80-81 0.3818363967948599 1101 82-83 0.3484991690583428 1101 84-85 0.3582437160309837 1101 86-87 0.34993304475599984 1101 88-89 0.36298535956353106 1101 90-91 0.3712082885100756 1101 92-93 0.4159692987695749 1101 94-95 0.4058607030314363 1101 96-97 0.4153510925553192 1101 98-99 0.4461555828845789 1101 100-101 0.52127746902271 1102 1 0.49673826736481885 1102 2 0.5880964557940231 1102 3 0.5059749801774203 1102 4 0.31410608712395316 1102 5 0.3190774308885622 1102 6 0.34194929589508405 1102 7 0.33065001142162487 1102 8 0.3378258131181511 1102 9 0.4110777480364689 1102 10-11 0.37202049402403503 1102 12-13 0.3669608307375185 1102 14-15 0.4733300215432408 1102 16-17 0.4810820540744629 1102 18-19 0.4829495662524721 1102 20-21 0.4947606258372872 1102 22-23 0.5043177775874099 1102 24-25 0.511925236192873 1102 26-27 0.5245757976616332 1102 28-29 0.5503451920287645 1102 30-31 0.5629300333209883 1102 32-33 0.6027913544382528 1102 34-35 0.6036566082446342 1102 36-37 0.6267008162382552 1102 38-39 0.5888066571189796 1102 40-41 0.5606181275808311 1102 42-43 0.5520643538485146 1102 44-45 0.5455833802446577 1102 46-47 0.4863693383771661 1102 48-49 0.5449287101599438 1102 50-51 0.5506122187074638 1102 52-53 0.5563740673661641 1102 54-55 0.5794985174153169 1102 56-57 0.5715601441016034 1102 58-59 0.5633241183376114 1102 60-61 0.577840061197108 1102 62-63 0.5385633221955572 1102 64-65 0.5154315014206574 1102 66-67 0.4606400109185529 1102 68-69 0.4463435052001259 1102 70-71 0.4226752240095877 1102 72-73 0.3759942979160229 1102 74-75 0.36839655982392827 1102 76-77 0.39964556738036805 1102 78-79 0.38723589175965856 1102 80-81 0.37407443533094664 1102 82-83 0.35127959490783667 1102 84-85 0.3603863091925916 1102 86-87 0.3549207726438368 1102 88-89 0.34718316088959256 1102 90-91 0.33911736599034725 1102 92-93 0.3646380451880944 1102 94-95 0.37955024111096236 1102 96-97 0.3667600283588186 1102 98-99 0.38687947516445576 1102 100-101 0.43499398715697524 1103 1 0.429644493295406 1103 2 0.5181526581739888 1103 3 0.4375710518803828 1103 4 0.2570862369165354 1103 5 0.2884440964774342 1103 6 0.29012043074556715 1103 7 0.24718562848173775 1103 8 0.23252201249155036 1103 9 0.3021051717237597 1103 10-11 0.31801908539032553 1103 12-13 0.32304810280802343 1103 14-15 0.4137907251808528 1103 16-17 0.40161618721608505 1103 18-19 0.416102518742953 1103 20-21 0.40010720848743375 1103 22-23 0.42181043279712327 1103 24-25 0.4183770965231943 1103 26-27 0.4108448146089074 1103 28-29 0.45469130300286764 1103 30-31 0.44830226859605915 1103 32-33 0.4717332899061617 1103 34-35 0.45025766484962304 1103 36-37 0.4464046210031043 1103 38-39 0.5025024454423175 1103 40-41 0.41601647907339157 1103 42-43 0.3944835083714935 1103 44-45 0.44587055276792853 1103 46-47 0.44645689855672543 1103 48-49 0.4309551478458644 1103 50-51 0.40939408715262715 1103 52-53 0.3391743200165678 1103 54-55 0.3813298784844932 1103 56-57 0.3790521680170684 1103 58-59 0.3768492852912573 1103 60-61 0.32490304994493613 1103 62-63 0.4023025319804532 1103 64-65 0.3711660932565053 1103 66-67 0.30665826651230077 1103 68-69 0.30672712820290116 1103 70-71 0.28389283104427676 1103 72-73 0.3087058421130138 1103 74-75 0.3038094808516405 1103 76-77 0.2765051255552322 1103 78-79 0.24537578101381996 1103 80-81 0.19967392657957106 1103 82-83 0.26691275980756046 1103 84-85 0.2407256091865122 1103 86-87 0.27531747939321605 1103 88-89 0.2361829175411927 1103 90-91 0.2635559469422404 1103 92-93 0.268397735690435 1103 94-95 0.2774591264401778 1103 96-97 0.26097333891287633 1103 98-99 0.28424768095443653 1103 100-101 0.33135356240363123 1104 1 0.33353352574984285 1104 2 0.44547566731095856 1104 3 0.364787750669862 1104 4 0.19850693587791568 1104 5 0.2519622885155357 1104 6 0.224838358984087 1104 7 0.2356096195025401 1104 8 0.24301710378913555 1104 9 0.2596774131102393 1104 10-11 0.3059993675273347 1104 12-13 0.29895643397642857 1104 14-15 0.3450320235829665 1104 16-17 0.3778370957430184 1104 18-19 0.3623425280415802 1104 20-21 0.36021098975513155 1104 22-23 0.37604012126412556 1104 24-25 0.37667460234611383 1104 26-27 0.38123533337742543 1104 28-29 0.38229732358951907 1104 30-31 0.38135067865049166 1104 32-33 0.42105319336839386 1104 34-35 0.41630039353095327 1104 36-37 0.39891884840215397 1104 38-39 0.40818010712295916 1104 40-41 0.3671496574853492 1104 42-43 0.3802626421924984 1104 44-45 0.38514854461317327 1104 46-47 0.3954404375428524 1104 48-49 0.3939785252814758 1104 50-51 0.3997832143822322 1104 52-53 0.4040720872260124 1104 54-55 0.3671515394309566 1104 56-57 0.40931490277822746 1104 58-59 0.35454027650806097 1104 60-61 0.3574578743232877 1104 62-63 0.31340350714476983 1104 64-65 0.27441841341133966 1104 66-67 0.2576104955892049 1104 68-69 0.28581249173113576 1104 70-71 0.30010496491716054 1104 72-73 0.2986175284446446 1104 74-75 0.27411482591103464 1104 76-77 0.2731666715458516 1104 78-79 0.27085193325627444 1104 80-81 0.20998620947359115 1104 82-83 0.20469480625882852 1104 84-85 0.2472704150819922 1104 86-87 0.21645203112669975 1104 88-89 0.21486095841209618 1104 90-91 0.25351483793515683 1104 92-93 0.2816959327284607 1104 94-95 0.2696836134574738 1104 96-97 0.25529747582501017 1104 98-99 0.3113581597367059 1104 100-101 0.3626531198318368 1105 1 0.2986481918627959 1105 2 0.4202159049335137 1105 3 0.3210296844632481 1105 4 0.20422166134859765 1105 5 0.18546426527155546 1105 6 0.21136971289327278 1105 7 0.18854786252015998 1105 8 0.19432547238116626 1105 9 0.22791282615212083 1105 10-11 0.2521604481794739 1105 12-13 0.25735022955663567 1105 14-15 0.30866466199838527 1105 16-17 0.2969091949640301 1105 18-19 0.30710997927122463 1105 20-21 0.30072639231792664 1105 22-23 0.32074323054175835 1105 24-25 0.3240571733606856 1105 26-27 0.3183890791429107 1105 28-29 0.3515456817026106 1105 30-31 0.3427547744818469 1105 32-33 0.3311670457297069 1105 34-35 0.34740071141810347 1105 36-37 0.33432021816738455 1105 38-39 0.3763175911828682 1105 40-41 0.31449933105872674 1105 42-43 0.342547645281968 1105 44-45 0.36868969222445713 1105 46-47 0.37160458380432715 1105 48-49 0.395102591868671 1105 50-51 0.394651668979769 1105 52-53 0.3764993813918167 1105 54-55 0.3986475831863743 1105 56-57 0.3679819156455011 1105 58-59 0.409620256526658 1105 60-61 0.37487474068981896 1105 62-63 0.34927167836188744 1105 64-65 0.2944331579994923 1105 66-67 0.28654557993620244 1105 68-69 0.26401672001959753 1105 70-71 0.23999678193440133 1105 72-73 0.262349493457819 1105 74-75 0.2529780761267446 1105 76-77 0.2661319993981479 1105 78-79 0.2834086461253591 1105 80-81 0.2661065023876006 1105 82-83 0.26231656442361384 1105 84-85 0.25112189563979825 1105 86-87 0.25636401916889184 1105 88-89 0.2493916205641611 1105 90-91 0.2726530266810343 1105 92-93 0.3133337847005677 1105 94-95 0.36351821184941 1105 96-97 0.3430394393444729 1105 98-99 0.3851342707484875 1105 100-101 0.46650166465901677 1106 1 0.22936774455587994 1106 2 0.3565158538504889 1106 3 0.2739587966906143 1106 4 0.18589966832673355 1106 5 0.17086126357725107 1106 6 0.18879154541427567 1106 7 0.16210239745298338 1106 8 0.1833095258242139 1106 9 0.21889966270445171 1106 10-11 0.21581490350077814 1106 12-13 0.22261168316779845 1106 14-15 0.27434224426088605 1106 16-17 0.24876430085457457 1106 18-19 0.25880234181450845 1106 20-21 0.24671830353727842 1106 22-23 0.2824637940757313 1106 24-25 0.2835044475069992 1106 26-27 0.267215499112325 1106 28-29 0.26789592824555086 1106 30-31 0.28123535380315445 1106 32-33 0.2859557193934634 1106 34-35 0.2767597011535301 1106 36-37 0.2863261388125906 1106 38-39 0.2804475034331091 1106 40-41 0.2576574625077086 1106 42-43 0.28023756847283465 1106 44-45 0.28947183021799816 1106 46-47 0.24376451589893833 1106 48-49 0.2656989264571692 1106 50-51 0.2696497729930982 1106 52-53 0.2572385056368276 1106 54-55 0.30734820910369365 1106 56-57 0.28110404824999335 1106 58-59 0.3044623718199517 1106 60-61 0.3012386305536978 1106 62-63 0.2568305485059099 1106 64-65 0.2683242860618833 1106 66-67 0.24074532570097062 1106 68-69 0.22044726058859254 1106 70-71 0.23109917547145642 1106 72-73 0.2525509667473429 1106 74-75 0.23277806514491317 1106 76-77 0.20120379109099673 1106 78-79 0.14838871284238309 1106 80-81 0.18186674491867194 1106 82-83 0.15195790469492465 1106 84-85 0.1747995848412387 1106 86-87 0.19048146316232817 1106 88-89 0.16817665198956178 1106 90-91 0.18410075321131814 1106 92-93 0.11158021983546718 1106 94-95 0.1355389044369133 1106 96-97 0.2024568209383304 1106 98-99 0.22996029788006211 1106 100-101 0.24076871275270761 1107 1 0.1593215870782032 1107 2 0.2998735109111692 1107 3 0.23802457039786873 1107 4 0.13737173475355036 1107 5 0.09457152754630016 1107 6 0.07449009305480558 1107 7 0.06630478908144255 1107 8 0.06134773568550855 1107 9 0.06682950512993102 1107 10-11 0.09141329065197823 1107 12-13 0.05515733123121436 1107 14-15 0.13134324699215227 1107 16-17 0.1724477304988028 1107 18-19 0.1780620157743087 1107 20-21 0.18367221865251793 1107 22-23 0.2042642756102353 1107 24-25 0.21073004682983054 1107 26-27 0.22365302980190194 1107 28-29 0.26502154024664293 1107 30-31 0.21944662416192529 1107 32-33 0.21134705597631864 1107 34-35 0.2645665353887736 1107 36-37 0.25523358745355296 1107 38-39 0.19764962047675283 1107 40-41 0.17997036268615574 1107 42-43 0.21403801528512645 1107 44-45 0.16239546543898342 1107 46-47 0.14744364650805153 1107 48-49 0.18474518411041174 1107 50-51 0.24227200458987852 1107 52-53 0.2363447160829395 1107 54-55 0.2221527310527165 1107 56-57 0.21626076203731515 1107 58-59 0.14782920388050513 1107 60-61 0.1727764427947065 1107 62-63 0.11322088603814962 1107 64-65 0.12035903186183816 1107 66-67 0.11985114369301186 1107 68-69 0.08587475128288702 1107 70-71 0.0764057450773592 1107 72-73 0.0509200451086258 1107 74-75 0.04335879008874599 1107 76-77 0.0680877686720649 1107 78-79 0.07630152253909728 1107 80-81 0.054071352161052744 1107 82-83 0.031185887876958418 1107 84-85 0.029035604462876563 1107 86-87 0.04546963654755842 1107 88-89 0.07714471106113052 1107 90-91 0.0795519232987445 1107 92-93 0.08474425424388699 1107 94-95 0.016813879316522673 1107 96-97 0.04735927370537496 1107 98-99 0.04829360394116122 1107 100-101 0.11179843553955138 1108 1 0.23427731347781844 1108 2 0.3444952220006243 1108 3 0.2810477215000091 1108 4 0.170431399529825 1108 5 0.13294376370363636 1108 6 0.11446234202460204 1108 7 0.12531995318728661 1108 8 0.138559047868128 1108 9 0.16439274323506226 1108 10-11 0.10526577905164913 1108 12-13 0.11797698247067956 1108 14-15 0.120378597394172 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-0.3622692481817751 2214 94-95 -0.4056550819761995 2214 96-97 -0.4001611489976682 2214 98-99 -0.4194155869738623 2214 100-101 -0.4406380381071209 2215 1 -0.4398895454676328 2215 2 -0.3094947577802216 2215 3 -0.35202633722110477 2215 4 -0.26268571232150606 2215 5 -0.3836984738800311 2215 6 -0.3937096754148044 2215 7 -0.4030746401743599 2215 8 -0.413821894179101 2215 9 -0.4720993797132351 2215 10-11 -0.5234878143949118 2215 12-13 -0.5350022615892556 2215 14-15 -0.6503296200231503 2215 16-17 -0.7105745869375539 2215 18-19 -0.7230432027201701 2215 20-21 -0.6914323914210101 2215 22-23 -0.6789854560585624 2215 24-25 -0.6667239987844269 2215 26-27 -0.7103542768354814 2215 28-29 -0.7261470795069584 2215 30-31 -0.7146686418071226 2215 32-33 -0.6957022080839792 2215 34-35 -0.6893818418999942 2215 36-37 -0.7232456538445646 2215 38-39 -0.706578041545562 2215 40-41 -0.7302989467810832 2215 42-43 -0.7283562913200541 2215 44-45 -0.7231926302829947 2215 46-47 -0.710277832594393 2215 48-49 -0.6761421052979841 2215 50-51 -0.7179330314698333 2215 52-53 -0.707120185419754 2215 54-55 -0.6923725584340374 2215 56-57 -0.666205677173302 2215 58-59 -0.6292432835174822 2215 60-61 -0.6039202393000096 2215 62-63 -0.586157675093105 2215 64-65 -0.5109056292296117 2215 66-67 -0.5271597290324479 2215 68-69 -0.5027648069697719 2215 70-71 -0.44487837019374155 2215 72-73 -0.4399318054405157 2215 74-75 -0.41158159485233625 2215 76-77 -0.3721021777371263 2215 78-79 -0.39702593448337353 2215 80-81 -0.38173356127715863 2215 82-83 -0.3332456484188384 2215 84-85 -0.3475607450619407 2215 86-87 -0.36258197683781646 2215 88-89 -0.32766996466369136 2215 90-91 -0.32428793092576313 2215 92-93 -0.3320134656935281 2215 94-95 -0.36090281085964904 2215 96-97 -0.3712358220213119 2215 98-99 -0.3702724905238881 2215 100-101 -0.47400251204565436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 19.0 13 159.0 14 689.0 15 1659.0 16 3096.0 17 5679.0 18 11900.0 19 27658.0 20 31272.0 21 28529.0 22 27070.0 23 28462.0 24 30086.0 25 34806.0 26 43727.0 27 56441.0 28 73463.0 29 96339.0 30 127771.0 31 171193.0 32 230972.0 33 319957.0 34 456543.0 35 681120.0 36 1135938.0 37 2286663.0 38 3167291.0 39 724460.0 40 722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.274049090595597 40.83929991679093 12.90485817043966 21.981792822173816 2 22.654855954816572 28.750832490344948 30.37207332912596 18.222238225712527 3 21.496172102632837 27.915135992231495 24.65009840687456 25.938593498261113 4 22.69155934732705 27.288932681945617 26.157592782713845 23.861915188013487 5 23.596688388090804 27.562625686157805 25.427173557697948 23.41351236805344 6 23.947913463151867 25.959544803816115 26.205503338795566 23.887038394236455 7 24.3156731371928 27.56117725120707 24.987461347442313 23.135688264157814 8 24.02857700956324 25.693807991586837 26.72290062359205 23.554714375257873 9 23.541811063900973 25.379579209246316 26.839112027773233 24.23949769907948 10-11 23.86420004314704 26.046456000983305 26.08444681770171 24.004897138167944 12-13 23.987164010267566 26.24624312184653 25.853467344167015 23.913125523718886 14-15 23.549058338777716 26.284927555301905 26.109238515925387 24.056775589994984 16-17 23.763039102133536 26.37879532033108 26.081024635124443 23.77714094241094 18-19 23.19048398637859 26.509807281649707 26.72907177250187 23.57063695946983 20-21 22.66476840086151 26.465395409991245 26.9880866225302 23.881749566617042 22-23 22.711597730853246 26.416862638895477 27.560274529424394 23.311265100826883 24-25 22.623906214856966 26.02452037167657 28.492602526498967 22.8589708869675 26-27 22.5209704310165 25.94648338864417 28.782534322553204 22.750011857786127 28-29 22.61082949931582 26.056044232347325 28.725275240891563 22.607851027445292 30-31 22.650870565506747 26.017778008983356 28.662268320534572 22.669083104975325 32-33 22.581034580364424 26.06150646415098 28.758777949311913 22.59868100617268 34-35 22.61762286323969 26.144286561634733 28.693501474190576 22.544589100935006 36-37 22.65005454581619 26.167696126507533 28.53960526067494 22.642644067001335 38-39 22.63516218646356 26.146770321567857 28.58227289024484 22.635794601723738 40-41 22.733232452899088 26.336464298883534 28.1701676461453 22.76013560207208 42-43 22.80240032191977 26.421901560484656 28.003827132348704 22.771870985246874 44-45 22.73938320131665 26.462014028398507 28.080971593844563 22.717631176440285 46-47 22.799896161494377 26.631149409635253 27.818758966653867 22.750195462216503 48-49 22.875648289393222 26.641201752198278 27.611107456022914 22.872042502385582 50-51 22.839289512055927 26.635051003780717 27.700635016322945 22.82502446784041 52-53 22.868977328422936 26.735436726088203 27.552609044456243 22.84297690103262 54-55 22.929490288600665 26.715326940839084 27.422739510704393 22.932443259855862 56-57 22.95627103482007 26.655757503428557 27.45942469591791 22.92854676583346 58-59 22.99009505099358 26.788564708066403 27.325969775650687 22.89537046528933 60-61 22.967266900150303 26.736344547993944 27.284184467371198 23.01220408448456 62-63 23.012081681530976 26.712169964661246 27.30011725182929 22.975631101978493 64-65 23.020496884589825 26.883896208415507 27.17446041972993 22.92114648726474 66-67 23.059849434166846 26.836852673255006 27.0432087526272 23.06008913995095 68-69 23.017839720472455 26.916480894683986 26.98496024709076 23.080719137752794 70-71 22.997296424762727 26.989336152681364 26.98206847731236 23.03129894524355 72-73 23.067091608920524 26.90046140813378 26.875537106710386 23.156909876235314 74-75 23.067433317165946 26.90865730590079 26.97656034440111 23.047349032532154 76-77 23.110881265564938 27.067566940390268 26.77919578199422 23.042356012050572 78-79 23.03927043759566 26.979482714917914 26.74870214618279 23.23254470130364 80-81 23.012918101713794 26.996802732849943 26.811530562232466 23.178748603203793 82-83 23.038250412982464 27.12321948328613 26.746299988218713 23.092230115512688 84-85 23.084248422914445 26.999939308535513 26.734646207012975 23.181166061537066 86-87 23.078719889510936 27.040373084202525 26.775564494371253 23.105342531915298 88-89 23.07992861867757 27.142737654259598 26.632036831048733 23.145296896014102 90-91 23.12803807955886 27.11602320964005 26.56384818565672 23.192090525144373 92-93 23.096432616919046 27.02182903673466 26.718840925631536 23.162897420714764 94-95 23.098814374390855 27.13697961531812 26.585034096872757 23.17917191341827 96-97 23.087823609183687 27.16415817113667 26.532166221170918 23.215851998508725 98-99 23.073981875182646 27.106848088244366 26.605261184952393 23.21390885162059 100-101 23.103353483919566 27.207320512643967 26.43921647829362 23.250109525142843 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1393.0 1 992.5 2 459.5 3 269.0 4 206.0 5 194.0 6 183.0 7 179.0 8 180.5 9 176.5 10 176.0 11 184.0 12 251.5 13 500.5 14 712.0 15 909.0 16 2812.5 17 3964.5 18 3747.0 19 4559.0 20 5839.0 21 7917.0 22 10859.0 23 14657.0 24 19434.0 25 25880.0 26 33791.0 27 43111.0 28 53353.5 29 65024.0 30 80441.5 31 97139.0 32 115600.5 33 139167.0 34 163801.0 35 190211.0 36 219266.5 37 246141.0 38 270079.0 39 295517.0 40 321546.0 41 343040.5 42 362062.0 43 382769.0 44 402648.5 45 416500.5 46 419904.5 47 418393.5 48 416861.5 49 410708.5 50 398768.0 51 388707.5 52 382445.5 53 370502.0 54 346914.0 55 316779.0 56 285306.5 57 250846.0 58 216886.5 59 181458.0 60 143859.5 61 113889.0 62 91929.5 63 71787.0 64 55762.0 65 42975.0 66 31362.5 67 22502.5 68 16639.5 69 12925.0 70 10493.0 71 8967.5 72 7790.0 73 6246.5 74 4664.0 75 3534.5 76 2546.0 77 1778.0 78 1179.5 79 650.0 80 265.5 81 136.5 82 103.5 83 49.5 84 11.5 85 2.0 86 2.5 87 1.5 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08035753902741673 2 0.002733665963359696 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9803685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.340267022172846 #Duplication Level Percentage of deduplicated Percentage of total 1 80.02684600754993 46.687875650227696 2 11.535523299627801 13.459710190815647 3 3.5724511759912176 6.252532665930091 4 1.5267229721358733 3.5627770345316874 5 0.7973404016117146 2.325852596879698 6 0.5096912396357316 1.784131381152652 7 0.33511267249346166 1.3685393957047756 8 0.2272953077891581 1.0608375159445158 9 0.1765848341963062 0.927180574117076 >10 1.1335688960350798 12.657714018678764 >50 0.11368732888969622 4.592670302872935 >100 0.044667737288563165 4.118611989815943 >500 2.6301021517642447E-4 0.09697099012183728 >1k 1.5757492626689913E-4 0.2124902260783786 >5k 3.501664565659203E-5 0.12911004493462774 >10k+ 5.2524968484888044E-5 0.7629954221935776 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50398 0.51407200455747 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14297 0.14583291894833422 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 10003 0.10203306205778745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0200246131939163E-5 0.0 0.0 0.0 0.0 2 9.180221518745248E-5 0.0 0.0 0.0 0.0 3 1.0200246131939164E-4 0.0 0.0 1.0200246131939163E-5 0.0 4 1.5300369197908745E-4 0.0 0.0 1.0200246131939163E-5 1.0200246131939163E-5 5 2.244054149026616E-4 0.0 0.0 1.0200246131939163E-5 1.0200246131939163E-5 6 4.896118143330798E-4 1.0200246131939163E-5 0.0 1.0200246131939163E-5 1.0200246131939163E-5 7 6.936167369718632E-4 1.0200246131939163E-5 0.0 2.0400492263878326E-5 1.0200246131939163E-5 8 9.180221518745248E-4 1.0200246131939163E-5 0.0 2.0400492263878326E-5 1.0200246131939163E-5 9 0.0018360443037490496 2.0400492263878326E-5 0.0 2.0400492263878326E-5 1.0200246131939163E-5 10-11 0.0041923011602269964 2.0400492263878326E-5 0.0 2.0400492263878326E-5 1.0200246131939163E-5 12-13 0.008078594936495818 3.060073839581749E-5 0.0 2.0400492263878326E-5 1.5300369197908745E-5 14-15 0.009659633086946388 3.060073839581749E-5 0.0 4.080098452775665E-5 2.0400492263878326E-5 16-17 0.011041766437824145 3.570086146178707E-5 0.0 4.080098452775665E-5 2.0400492263878326E-5 18-19 0.012582003603746958 4.590110759372624E-5 0.0 4.080098452775665E-5 2.0400492263878326E-5 20-21 0.014086539908207985 5.61013537256654E-5 0.0 4.080098452775665E-5 2.0400492263878326E-5 22-23 0.02084930309368365 6.120147679163498E-5 0.0 4.080098452775665E-5 2.0400492263878326E-5 24-25 0.027846671940193916 7.140172292357414E-5 0.0 5.61013537256654E-5 2.550061532984791E-5 26-27 0.031911470023771676 8.160196905551332E-5 0.0 6.120147679163498E-5 3.060073839581749E-5 28-29 0.03577226318471065 1.122027074513308E-4 0.0 8.16019690555133E-5 3.060073839581749E-5 30-31 0.0375573062578 1.2750307664923953E-4 0.0 9.690233825342206E-5 3.060073839581749E-5 32-33 0.0396024556072538 1.5810381504505704E-4 0.0 1.122027074513308E-4 3.060073839581749E-5 34-35 0.04171900667963118 1.7340418424296577E-4 0.0 1.3770332278117872E-4 3.060073839581749E-5 36-37 0.04517178999529259 2.0400492263878328E-4 0.0 1.4280344584714828E-4 3.060073839581749E-5 38-39 0.049552795708960456 2.1420516877072244E-4 0.0 1.5300369197908745E-4 3.060073839581749E-5 40-41 0.05419900782205875 2.244054149026616E-4 0.0 1.5300369197908745E-4 3.060073839581749E-5 42-43 0.06068126423890609 2.244054149026616E-4 0.0 1.5810381504505704E-4 3.060073839581749E-5 44-45 0.06847935240677358 2.244054149026616E-4 0.0 1.632039381110266E-4 3.060073839581749E-5 46-47 0.07866429816951483 2.244054149026616E-4 0.0 1.632039381110266E-4 3.060073839581749E-5 48-49 0.09240402970923689 2.244054149026616E-4 0.0 1.683040611769962E-4 3.060073839581749E-5 50-51 0.10885702672005476 2.295055379686312E-4 0.0 1.7850430730893536E-4 3.060073839581749E-5 52-53 0.1290688144304922 2.3460566103460076E-4 0.0 1.8360443037490496E-4 3.060073839581749E-5 54-55 0.1528965893947021 2.448059071665399E-4 0.0 1.8360443037490496E-4 3.060073839581749E-5 56-57 0.18114617105710762 2.6010627636444865E-4 0.0 1.8870455344087455E-4 3.060073839581749E-5 58-59 0.2144958757854827 2.6520639943041825E-4 0.0 1.9380467650684412E-4 3.060073839581749E-5 60-61 0.2544706403765523 2.6520639943041825E-4 0.0 2.0400492263878328E-4 3.060073839581749E-5 62-63 0.2999076367712753 2.6520639943041825E-4 0.0 2.19305291836692E-4 3.060073839581749E-5 64-65 0.3527194111193903 2.7030652249638784E-4 0.0 2.448059071665399E-4 4.590110759372624E-5 66-67 0.41308956785127227 2.7540664556235744E-4 0.0 2.550061532984791E-4 5.100123065969582E-5 68-69 0.48167092271936524 2.8050676862832703E-4 0.0 2.6010627636444865E-4 5.100123065969582E-5 70-71 0.5575454535717947 2.8560689169429657E-4 0.0 2.6520639943041825E-4 6.120147679163498E-5 72-73 0.6441200426166283 2.8560689169429657E-4 0.0 2.7030652249638784E-4 8.16019690555133E-5 74-75 0.7410631818545781 2.9580713782623576E-4 0.0 2.7540664556235744E-4 8.16019690555133E-5 76-77 0.8487114794079982 2.9580713782623576E-4 0.0 2.7540664556235744E-4 8.16019690555133E-5 78-79 0.9665141219857636 2.9580713782623576E-4 0.0 2.7540664556235744E-4 8.16019690555133E-5 80-81 1.0974903824429283 2.9580713782623576E-4 0.0 3.009072608922053E-4 8.16019690555133E-5 82-83 1.2417065623793502 2.9580713782623576E-4 0.0 3.264078762220532E-4 8.16019690555133E-5 84-85 1.3971532133070372 2.9580713782623576E-4 0.0 3.264078762220532E-4 8.16019690555133E-5 86-87 1.5656918801450679 2.9580713782623576E-4 0.0 3.366081223539924E-4 8.16019690555133E-5 88-89 1.7495207159348753 2.9580713782623576E-4 0.0 3.366081223539924E-4 8.16019690555133E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6540 0.0 84.60916 1 TCGACGG 6995 0.0 79.382225 2 ATCTCGA 13000 0.0 63.591393 1 CGACGGC 8905 0.0 62.834938 3 TCTCGAG 14405 0.0 56.485966 2 GACGGCG 11270 0.0 49.564785 4 ACGGCGC 11265 0.0 49.50245 5 TCGAGGG 16745 0.0 48.733524 4 CTCGAGG 17105 0.0 47.735626 3 CGAGGGC 17685 0.0 46.277515 5 CGGCGCG 12115 0.0 45.99011 6 GGGCGCG 18265 0.0 44.755974 8 AGGGCGC 18685 0.0 43.90248 7 GAGGGCG 18750 0.0 43.62362 6 TATCTCG 4900 0.0 31.408367 2 ATATCTC 5905 0.0 26.969135 1 GGCGCGC 31225 0.0 26.651491 9 CATATGT 7555 0.0 25.96602 4 ACATATG 7770 0.0 25.614319 3 CGCGCCG 30460 0.0 25.5743 9 >>END_MODULE