FastQCFastQC Report
Tue 31 May 2016
SRR2048244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321790
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT100330.3020359504965696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37620.11325219234208063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG133950.086.279491
TATCTCG67800.083.203652
ATATCTC69600.081.2836151
ATCTCGA69850.080.761743
TCGACGG146550.078.867692
CGACGGC147750.078.2271353
TCTCGAG54200.077.424114
CGGCGCG153050.075.49096
TCGAGGG55650.075.2099156
CTCGAGG56100.074.702565
AGGGCGC56050.074.4834759
CGAGGGC56350.074.020487
GACGGCG157450.073.376514
ACGGCGC157650.073.269085
GAGGGCG61400.068.462748
CTCGACG21200.067.686315
CGCGCCG203950.056.8913769
GGCGCGC206600.056.2211657
GCGCGCC206700.056.1387028
TCTCGAC26000.055.2011834
Produced by FastQC (version 0.11.5)