##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048244_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3321790 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98608129954031 31.0 31.0 34.0 28.0 34.0 2 31.282186712585684 31.0 31.0 34.0 28.0 34.0 3 31.339659039252933 31.0 31.0 34.0 28.0 34.0 4 35.12059130769856 37.0 35.0 37.0 32.0 37.0 5 35.0752690567435 37.0 35.0 37.0 32.0 37.0 6 35.070800983806926 37.0 35.0 37.0 32.0 37.0 7 34.819597867414856 37.0 35.0 37.0 31.0 37.0 8 34.90716902633821 37.0 35.0 37.0 32.0 37.0 9 36.535628080041185 39.0 37.0 39.0 32.0 39.0 10-11 36.45378606112969 39.0 36.0 39.0 32.0 39.0 12-13 36.262133066810364 38.5 35.0 39.0 31.5 39.0 14-15 37.513602154260205 40.0 37.0 41.0 32.0 41.0 16-17 37.583310955840076 40.0 37.0 41.0 31.5 41.0 18-19 37.48897552223349 40.0 37.0 41.0 31.5 41.0 20-21 37.50575894322037 40.0 37.0 41.0 31.0 41.0 22-23 37.66284352713447 40.0 37.0 41.0 32.0 41.0 24-25 37.492739607259935 40.0 37.0 41.0 31.0 41.0 26-27 37.57296126486021 40.0 37.0 41.0 31.0 41.0 28-29 37.471783285517745 40.0 37.0 41.0 31.0 41.0 30-31 37.202356861812454 39.0 36.0 41.0 31.0 41.0 32-33 36.97718203137465 39.0 35.5 40.5 30.5 41.0 34-35 36.93790305829086 39.0 35.0 41.0 30.5 41.0 36-37 36.58504586382644 38.5 35.0 40.0 30.0 41.0 38-39 36.425206891465145 38.5 35.0 40.0 30.0 41.0 40-41 36.21433188130496 38.5 35.0 40.0 28.5 41.0 42-43 36.193367882978755 39.0 35.0 40.0 28.5 41.0 44-45 35.947826924640026 38.0 34.0 40.0 28.0 41.0 46-47 35.823885164324054 38.0 34.0 40.0 28.0 41.0 48-49 35.688962878448066 38.0 34.0 40.0 27.0 41.0 50-51 35.627786223692645 38.0 34.0 40.0 27.5 41.0 52-53 35.42033512052237 38.0 33.0 40.0 27.0 41.0 54-55 35.326586870331965 38.0 33.0 40.0 27.0 41.0 56-57 35.219713317217526 38.0 33.0 40.0 27.0 41.0 58-59 35.07912420712928 37.5 33.0 40.0 27.0 41.0 60-61 34.831935041047146 37.0 33.0 40.0 27.0 41.0 62-63 34.508268132542995 36.5 33.0 40.0 26.0 41.0 64-65 34.159449573874326 36.0 33.0 39.0 26.0 41.0 66-67 33.817773850845484 35.5 32.0 39.0 26.0 41.0 68-69 33.46366973830375 35.0 32.0 39.0 26.0 40.0 70-71 33.08087085577354 35.0 32.0 38.0 25.0 40.0 72-73 32.806653792081974 35.0 32.0 37.0 25.0 39.5 74-75 32.8034296267976 35.0 32.0 37.0 26.0 39.0 76-77 32.54083205139398 35.0 32.0 36.0 25.5 39.0 78-79 32.25493258152984 35.0 32.0 36.0 25.0 38.0 80-81 31.979239355889447 35.0 32.0 35.5 25.0 37.0 82-83 31.716812170546604 35.0 32.0 35.0 25.0 37.0 84-85 31.507316687689467 35.0 32.0 35.0 25.0 36.0 86-87 31.31513927731735 35.0 32.0 35.0 24.0 36.0 88-89 31.141071831753365 34.0 31.5 35.0 24.0 36.0 90-91 30.964935923101702 34.0 31.0 35.0 23.5 35.5 92-93 30.808273701829435 34.0 31.0 35.0 23.0 35.0 94-95 30.658941263595832 34.0 31.0 35.0 21.5 35.0 96-97 30.48435873429687 34.0 31.0 35.0 20.0 35.0 98-99 30.289560447830837 34.0 31.0 35.0 18.5 35.0 100-101 29.654861686018684 34.0 30.0 35.0 8.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5255452889323244 1101 2 0.5249531012987774 1101 3 0.5194524978672632 1101 4 0.4343895591577791 1101 5 0.4218687296449488 1101 6 0.4851117214957199 1101 7 0.5507288292941368 1101 8 0.6349953318309431 1101 9 0.626306435072884 1101 10-11 0.6170147487974518 1101 12-13 0.5877778661330666 1101 14-15 0.7097631901720547 1101 16-17 0.7067212553986124 1101 18-19 0.7404351764713226 1101 20-21 0.8388470222985944 1101 22-23 0.7726415810568099 1101 24-25 0.7008849850180923 1101 26-27 0.6673010295402548 1101 28-29 0.8683471176237276 1101 30-31 0.8058042480726044 1101 32-33 0.7405288195380351 1101 34-35 0.8331564644945431 1101 36-37 0.8118139626724599 1101 38-39 0.8049634742817915 1101 40-41 0.6081122705208131 1101 42-43 0.7725797599397382 1101 44-45 0.7762220506608344 1101 46-47 0.7755265484251765 1101 48-49 0.8074519725673497 1101 50-51 0.6709075770224118 1101 52-53 0.5988321596825728 1101 54-55 0.5300438176107534 1101 56-57 0.6079750326863262 1101 58-59 0.6414686876768414 1101 60-61 0.5215223520359729 1101 62-63 0.4638957881794852 1101 64-65 0.4434710271128637 1101 66-67 0.4163965978651092 1101 68-69 0.4245768063147253 1101 70-71 0.4797300702429297 1101 72-73 0.5259343441307962 1101 74-75 0.48181653726770435 1101 76-77 0.3812618241016139 1101 78-79 0.4187268956379384 1101 80-81 0.40823878667678315 1101 82-83 0.45201928248592793 1101 84-85 0.47772114176638425 1101 86-87 0.3574052593794903 1101 88-89 0.39749523356912775 1101 90-91 0.373722813344866 1101 92-93 0.4475069598553105 1101 94-95 0.42094978150254647 1101 96-97 0.4681147671203796 1101 98-99 0.4772543348841971 1101 100-101 0.5375376454609722 1102 1 0.38442012911470513 1102 2 0.3545001922775697 1102 3 0.3861680098577196 1102 4 0.3175227434253003 1102 5 0.4111725037732725 1102 6 0.4077281280756324 1102 7 0.42891861381036733 1102 8 0.5070588959438354 1102 9 0.5895255170532465 1102 10-11 0.5284991406731621 1102 12-13 0.550128471931842 1102 14-15 0.6677410083623556 1102 16-17 0.6534135658602693 1102 18-19 0.7039943269908164 1102 20-21 0.7831480282008982 1102 22-23 0.7146738214030464 1102 24-25 0.5923185904062578 1102 26-27 0.5814285542694151 1102 28-29 0.7217563533792841 1102 30-31 0.609391010308741 1102 32-33 0.6023086550791348 1102 34-35 0.6875731958666691 1102 36-37 0.717453761005487 1102 38-39 0.674417292098461 1102 40-41 0.5439866674951261 1102 42-43 0.47974760444036235 1102 44-45 0.4741832327371256 1102 46-47 0.47142627782986324 1102 48-49 0.5240811052028391 1102 50-51 0.39212532402699196 1102 52-53 0.41421927590135255 1102 54-55 0.38351666418389385 1102 56-57 0.3251929606715862 1102 58-59 0.28636865692958935 1102 60-61 0.3276584120476116 1102 62-63 0.280336010065696 1102 64-65 0.17485896406930834 1102 66-67 0.24422346016604735 1102 68-69 0.1796418936816977 1102 70-71 0.18582176079710422 1102 72-73 0.1952859832708853 1102 74-75 0.1299520227143276 1102 76-77 0.14148601635369573 1102 78-79 0.1438965407017463 1102 80-81 0.09447143539717473 1102 82-83 0.09213834539520249 1102 84-85 0.13470655822628785 1102 86-87 0.10390465757007306 1102 88-89 0.1520894427711248 1102 90-91 0.14844528530745293 1102 92-93 0.30691124150796156 1102 94-95 0.13912801390509344 1102 96-97 0.12500044960242107 1102 98-99 0.03171218827817057 1102 100-101 0.0862080699093184 1103 1 0.302134574473758 1103 2 0.2931655351945004 1103 3 0.37559120149747116 1103 4 0.2911151751899368 1103 5 0.3857202575853975 1103 6 0.35778375394207274 1103 7 0.39332773161629575 1103 8 0.42037910768783604 1103 9 0.46761584019086655 1103 10-11 0.5231589375902885 1103 12-13 0.48150056131612473 1103 14-15 0.6611066920289375 1103 16-17 0.5707420974033397 1103 18-19 0.5971138459644649 1103 20-21 0.6516546350590176 1103 22-23 0.569733497056383 1103 24-25 0.5144186961823323 1103 26-27 0.48799378541502847 1103 28-29 0.6215464798929062 1103 30-31 0.558759601268676 1103 32-33 0.6206076566512877 1103 34-35 0.6901992923217435 1103 36-37 0.6738648044835926 1103 38-39 0.6943888066987824 1103 40-41 0.5781931081051468 1103 42-43 0.5437010038132968 1103 44-45 0.5710646869913276 1103 46-47 0.5551605630479699 1103 48-49 0.5448072610707726 1103 50-51 0.5168432328381414 1103 52-53 0.5042319998062084 1103 54-55 0.38940926899123696 1103 56-57 0.2594504607710064 1103 58-59 0.38295898216554036 1103 60-61 0.37172157233622727 1103 62-63 0.2718148707376429 1103 64-65 0.19825336295323837 1103 66-67 0.3282565882822013 1103 68-69 0.35636544121907576 1103 70-71 0.3162763980878722 1103 72-73 0.3217804425668831 1103 74-75 0.30476060958150697 1103 76-77 0.27301480141562706 1103 78-79 0.2956732410676679 1103 80-81 0.23232272418464817 1103 82-83 0.2663688309279806 1103 84-85 0.25196160806974177 1103 86-87 0.2615008407583339 1103 88-89 0.24816591569750202 1103 90-91 0.2564121643855941 1103 92-93 0.31768897045780875 1103 94-95 0.36002533397135394 1103 96-97 0.36452061430374627 1103 98-99 0.33645010921170027 1103 100-101 0.394825732655697 1104 1 0.2298280107988333 1104 2 0.2276464369120461 1104 3 0.22361971868563302 1104 4 0.19928167373734595 1104 5 0.20200642842708305 1104 6 0.22415899221940805 1104 7 0.29952616176615976 1104 8 0.28087648053993064 1104 9 0.2960389856273551 1104 10-11 0.3259587573092162 1104 12-13 0.38789419840875894 1104 14-15 0.5201472806349443 1104 16-17 0.5003066327600862 1104 18-19 0.42526725267193655 1104 20-21 0.5624784259173623 1104 22-23 0.47681469963261236 1104 24-25 0.3636318200202098 1104 26-27 0.42918469409797666 1104 28-29 0.5576636664935748 1104 30-31 0.4598471409978231 1104 32-33 0.4381441860064754 1104 34-35 0.5011743905105348 1104 36-37 0.5941289630841169 1104 38-39 0.522513142107421 1104 40-41 0.45829858424033887 1104 42-43 0.4747151043687765 1104 44-45 0.47979153970129573 1104 46-47 0.5187710037415485 1104 48-49 0.5555498864236768 1104 50-51 0.43775378884136984 1104 52-53 0.44792069887533614 1104 54-55 0.4328597394975233 1104 56-57 0.4350602263860992 1104 58-59 0.40521901225141477 1104 60-61 0.42203735688083555 1104 62-63 0.39918852277565264 1104 64-65 0.4115748466430631 1104 66-67 0.44601905430877764 1104 68-69 0.4207641238404918 1104 70-71 0.4439756876945893 1104 72-73 0.36923777702143923 1104 74-75 0.3813706034745863 1104 76-77 0.36776284690733263 1104 78-79 0.37697932282241453 1104 80-81 0.26812588222520617 1104 82-83 0.34931636796462584 1104 84-85 0.414460608134128 1104 86-87 0.3809272972011364 1104 88-89 0.3103623986556734 1104 90-91 0.2956710999077927 1104 92-93 0.3341661533904201 1104 94-95 0.33387667622638517 1104 96-97 0.40316866233187554 1104 98-99 0.4426036642023057 1104 100-101 0.4768661085924677 1105 1 0.1632880073789238 1105 2 0.21513109482628323 1105 3 0.24851398654040224 1105 4 0.20377467242638403 1105 5 0.28024251605271644 1105 6 0.333703954505431 1105 7 0.18305892638151278 1105 8 0.160602127947449 1105 9 0.3314844286003975 1105 10-11 0.3980827099624946 1105 12-13 0.36030618328516795 1105 14-15 0.31705283418499164 1105 16-17 0.5099655919681965 1105 18-19 0.4353542703224562 1105 20-21 0.466727281198267 1105 22-23 0.4077508043577822 1105 24-25 0.32204194294688904 1105 26-27 0.38672255057585403 1105 28-29 0.48878805613505705 1105 30-31 0.4470380668428362 1105 32-33 0.3501121528713682 1105 34-35 0.46541374604793617 1105 36-37 0.5288811147760129 1105 38-39 0.4670184904630261 1105 40-41 0.37280887246255645 1105 42-43 0.40196891851579863 1105 44-45 0.43447852315274105 1105 46-47 0.4115257393636895 1105 48-49 0.44087430266444017 1105 50-51 0.35932181650461814 1105 52-53 0.43300287165668294 1105 54-55 0.45084883379198004 1105 56-57 0.3343342559873008 1105 58-59 0.2876418268354186 1105 60-61 0.20913157533694005 1105 62-63 0.2629006425484164 1105 64-65 0.16316358374716344 1105 66-67 0.3290300930107364 1105 68-69 0.20253392605582832 1105 70-71 0.324459034644228 1105 72-73 0.35639146387190124 1105 74-75 0.2958375728664109 1105 76-77 0.34532331277475947 1105 78-79 0.3237754393272567 1105 80-81 0.2946708255117372 1105 82-83 0.2720435543305868 1105 84-85 0.281200106167681 1105 86-87 0.2910743532306235 1105 88-89 0.34431562191864273 1105 90-91 0.28934579069114363 1105 92-93 0.36297621551874215 1105 94-95 0.26161725723070006 1105 96-97 0.30502415223364565 1105 98-99 0.2823878300298297 1105 100-101 0.4253531794415544 1106 1 0.02929141626651699 1106 2 0.11629614336997207 1106 3 0.20855786890552963 1106 4 0.12120821324357678 1106 5 0.06011124562999015 1106 6 0.128266872965213 1106 7 0.15027164285058348 1106 8 0.15627133401564208 1106 9 0.20318971990961643 1106 10-11 0.2547644121462511 1106 12-13 0.23884704756058994 1106 14-15 0.36842740689694153 1106 16-17 0.2858229460310753 1106 18-19 0.22165506227673148 1106 20-21 0.3451271757299281 1106 22-23 0.2535578726202843 1106 24-25 0.1615054669079825 1106 26-27 0.17793298817531422 1106 28-29 0.3757314516507648 1106 30-31 0.30808427516164727 1106 32-33 0.2956663796094787 1106 34-35 0.34212963127577467 1106 36-37 0.4047075816806114 1106 38-39 0.43239003096004325 1106 40-41 0.4064690948877683 1106 42-43 0.29352485911451254 1106 44-45 0.3134419199868077 1106 46-47 0.33620994642064517 1106 48-49 0.34819747839165416 1106 50-51 0.3027678063532946 1106 52-53 0.3278930244916154 1106 54-55 0.24980432050013945 1106 56-57 0.15920792939326134 1106 58-59 0.11240372262892606 1106 60-61 0.09687530609132011 1106 62-63 0.0895148261575116 1106 64-65 0.04005782348048115 1106 66-67 0.07525187787414467 1106 68-69 0.10770714839628681 1106 70-71 0.13976625828420453 1106 72-73 0.06622064281966544 1106 74-75 0.11969611344817821 1106 76-77 0.2330132735066286 1106 78-79 0.2381335420270645 1106 80-81 0.19245561358628294 1106 82-83 0.16322046806313395 1106 84-85 0.15879561622955052 1106 86-87 0.04236803548839774 1106 88-89 0.04296986466534136 1106 90-91 -0.09024495446154646 1106 92-93 -0.0023676395376170944 1106 94-95 0.10146338644825548 1106 96-97 0.1530474682714278 1106 98-99 0.15458223079070876 1106 100-101 0.1762932602378413 1107 1 0.0989588266047754 1107 2 0.12867131470343907 1107 3 0.16814871595234493 1107 4 0.1892884446902272 1107 5 0.1551748856729347 1107 6 0.18634584522842346 1107 7 0.33033886387450906 1107 8 -0.07930445372895178 1107 9 0.0061445657432059875 1107 10-11 0.10006628096061121 1107 12-13 -0.010587127057952728 1107 14-15 -0.12267478125241382 1107 16-17 -0.0904033286642516 1107 18-19 0.05601747578383964 1107 20-21 0.05223687111958952 1107 22-23 -0.004304170633709248 1107 24-25 -0.02542945078073444 1107 26-27 0.12871739488815592 1107 28-29 0.019988065260164944 1107 30-31 0.09476441236895994 1107 32-33 0.09484378906079627 1107 34-35 0.23855936190738447 1107 36-37 0.36876465554720284 1107 38-39 0.2512917419111531 1107 40-41 -0.09857523577646532 1107 42-43 0.03229014143274611 1107 44-45 0.021656069296334124 1107 46-47 0.0996547874435123 1107 48-49 0.24955682674617208 1107 50-51 0.12979546679614629 1107 52-53 0.22923056979040268 1107 54-55 0.10669500723371783 1107 56-57 -0.08952017187775141 1107 58-59 0.09769290844622702 1107 60-61 -0.12050281188334111 1107 62-63 0.035999016947926066 1107 64-65 -0.1680783058351949 1107 66-67 0.07570125252718185 1107 68-69 0.255237183150129 1107 70-71 0.39827631570334887 1107 72-73 0.38138519986343056 1107 74-75 0.3501511268880151 1107 76-77 0.36271923423036156 1107 78-79 0.40820117518191523 1107 80-81 0.4800452117054448 1107 82-83 0.3882279842297791 1107 84-85 0.4243092215832256 1107 86-87 0.40062931396412793 1107 88-89 0.43477214361644556 1107 90-91 0.30826032071941256 1107 92-93 0.27413523768706227 1107 94-95 0.24575697788823803 1107 96-97 0.27425899266777165 1107 98-99 0.22519292716473416 1107 100-101 0.24970669384497057 1108 1 -0.1706748550666326 1108 2 -0.16535611407478967 1108 3 -0.010220240257954316 1108 4 -0.12839267972753987 1108 5 -0.016691910329683424 1108 6 0.03508537453657823 1108 7 0.09733310263101203 1108 8 0.1862356207614866 1108 9 0.22409702104791052 1108 10-11 0.14498682568393662 1108 12-13 0.22220045969974223 1108 14-15 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-0.628651812135665 2215 7 -0.7163640844602455 2215 8 -0.5767249694055394 2215 9 -0.7490841650163915 2215 10-11 -0.6473180378350705 2215 12-13 -0.5964713961613555 2215 14-15 -0.7762371593326094 2215 16-17 -0.7414237772703203 2215 18-19 -0.612787881796585 2215 20-21 -3.600360450352433 2215 22-23 -1.39502598382753 2215 24-25 -0.93778381241966 2215 26-27 -0.7971452493650801 2215 28-29 -0.7161143085472048 2215 30-31 -1.1655360024501107 2215 32-33 -1.0304477480166696 2215 34-35 -1.14598017387123 2215 36-37 -1.1930366720701002 2215 38-39 -0.9455412023938905 2215 40-41 -1.190787056814294 2215 42-43 -1.534734456974796 2215 44-45 -1.1031975463965225 2215 46-47 -0.9120432015907696 2215 48-49 -0.9314300877208126 2215 50-51 -0.868256757033457 2215 52-53 -0.6282857139360516 2215 54-55 -0.5602131093300855 2215 56-57 -0.47650440659552373 2215 58-59 -0.33700469672317723 2215 60-61 -0.2662440601331113 2215 62-63 -0.3678779696240042 2215 64-65 -0.31106580027642394 2215 66-67 -0.4800747788179578 2215 68-69 -0.42637623236357314 2215 70-71 -0.23096576675106917 2215 72-73 -0.2290860511221311 2215 74-75 -0.27157050802797045 2215 76-77 -0.3492734133355597 2215 78-79 -0.34170252144772917 2215 80-81 -0.3815027021573272 2215 82-83 -0.394732483456778 2215 84-85 -0.40648408030992655 2215 86-87 -0.46240727037793405 2215 88-89 -0.47279469586746004 2215 90-91 -0.36463746651890006 2215 92-93 -0.27076256798216036 2215 94-95 -0.351398878182696 2215 96-97 -0.35035618251070844 2215 98-99 -0.3202911649569913 2215 100-101 -0.3030225247530609 2216 1 -0.42349086580771456 2216 2 -0.5447077176338873 2216 3 -0.5062819050516083 2216 4 -0.4154879882925897 2216 5 -0.44189265656789445 2216 6 -0.46111630264492476 2216 7 -0.6202442936207362 2216 8 -1.6592726705681287 2216 9 -0.9326382238717699 2216 10-11 -0.783352608851466 2216 12-13 -0.7548928645837876 2216 14-15 -1.519913278946163 2216 16-17 -0.803785766880253 2216 18-19 -0.8742435105374611 2216 20-21 -0.9287536574380084 2216 22-23 -0.6776135544531812 2216 24-25 -0.9252347373395011 2216 26-27 -1.2397950488543685 2216 28-29 -1.9066445262392762 2216 30-31 -1.0920238591608467 2216 32-33 -0.9428430145012783 2216 34-35 -2.3351997037402654 2216 36-37 -2.2147844023361003 2216 38-39 -2.310019782206318 2216 40-41 -1.4689651276124138 2216 42-43 -1.4638949342412602 2216 44-45 -1.210919572585162 2216 46-47 -1.4384776343290113 2216 48-49 -1.4516921403388992 2216 50-51 -1.0352178618700165 2216 52-53 -0.7691298644081002 2216 54-55 -0.7611795052266572 2216 56-57 -0.8329740995249466 2216 58-59 -0.8573609845751591 2216 60-61 -0.9997654145794712 2216 62-63 -0.8961386047432569 2216 64-65 -0.746906139235179 2216 66-67 -0.8166958132006101 2216 68-69 -0.815707817768434 2216 70-71 -1.0322103809572312 2216 72-73 -0.7829875892450247 2216 74-75 -0.7719337282544032 2216 76-77 -0.8614816128165756 2216 78-79 -0.9270747312316452 2216 80-81 -0.9001200055412291 2216 82-83 -1.101681939540537 2216 84-85 -0.7432151204320121 2216 86-87 -0.9818959957591424 2216 88-89 -0.7996272877038777 2216 90-91 -0.9595425775661539 2216 92-93 -1.1279669190166253 2216 94-95 -0.8094366468814549 2216 96-97 -0.7637285186064027 2216 98-99 -0.9132737885929529 2216 100-101 -0.9400760292400818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 60.0 11 656.0 12 2611.0 13 5607.0 14 7869.0 15 9677.0 16 10948.0 17 12530.0 18 16815.0 19 23005.0 20 22551.0 21 21948.0 22 25368.0 23 30404.0 24 35225.0 25 40747.0 26 47706.0 27 55914.0 28 66058.0 29 77100.0 30 88668.0 31 104234.0 32 125767.0 33 156771.0 34 203544.0 35 288433.0 36 472610.0 37 710164.0 38 576335.0 39 82419.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.754405364322913 39.40505549169696 12.115493836180857 20.72504530779927 2 24.062277603114605 26.936063238944946 32.10204077330192 16.89961838463853 3 22.246692320110643 27.59439929256251 23.027680660403497 27.131227726923346 4 25.844648069705023 25.593588786279724 25.261202560413114 23.300560583602138 5 23.331224177014935 28.71559608160718 23.92753015185022 24.02564958952766 6 22.634020176229626 24.627465385617096 26.198838685007235 26.539675753146042 7 26.510395197255367 25.40880268320656 23.575163778518025 24.505638341020042 8 27.267841626678724 24.318167722340277 25.170333875471258 23.243656775509738 9 22.14674563409717 25.75215092721905 25.433639992931344 26.66746344575244 10-11 26.09180858695649 24.794529043196693 24.36221291189397 24.75144945795285 12-13 25.28520921965746 25.066440442029137 24.200291290311316 25.448059048002094 14-15 25.142978077372156 24.863592253542222 24.52896447219621 25.464465196889414 16-17 25.282141015943978 26.63108341517933 24.350774996895495 23.7360005719812 18-19 23.37748957432541 24.84205883836046 26.292523995421057 25.487927591893072 20-21 23.212756493289007 27.440023302005585 24.666015876372928 24.681204328332484 22-23 22.384522579718585 26.82910820145384 27.287245237298784 23.49912398152879 24-25 22.282457245704247 25.296045128844387 28.919299972976287 23.50219765247508 26-27 22.270593070184812 25.129454198398587 30.087085590759443 22.512867140657153 28-29 22.34213713577708 24.826858384663968 30.4109285882577 22.420075891301256 30-31 22.412157346480914 24.843701903574217 30.29990720329703 22.44423354664784 32-33 22.27427889655974 24.85174660896946 30.42554725615883 22.44842723831197 34-35 22.402941794051838 24.98897805620928 30.38171523873101 22.22636491100787 36-37 22.356136208526898 24.9969217578584 30.168579291152337 22.47836274246237 38-39 22.54153694877013 25.07333724625885 29.87033905614998 22.51478674882104 40-41 23.064233896368684 25.607579188840745 28.174361081696198 23.153825833094377 42-43 23.12605065797079 25.813537411853112 27.9902057001657 23.070206230010402 44-45 23.296523527232697 26.03652421061473 27.579748349835604 23.087203912316966 46-47 23.256647771231776 26.513566480722744 27.36425240608226 22.865533341963218 48-49 23.229538893187108 26.61027638712863 27.09608373798464 23.064100981699625 50-51 23.20018376323564 26.59853919667709 27.086366763902024 23.114910276185245 52-53 23.291093511394365 26.764171739986136 26.88919779962565 23.055536948993854 54-55 23.308959809953052 26.604540950424415 26.805326360208632 23.2811728794139 56-57 23.359709674603153 26.54118713103477 26.95501521769889 23.144087976663187 58-59 23.458906996431878 26.633080807168515 26.7795146728901 23.128497523509502 60-61 23.354667212557086 26.609192634091862 26.822481252577678 23.21365890077338 62-63 23.355540235836703 26.489106776767947 26.982334825500708 23.17301816189464 64-65 23.435491676381755 26.702787484505404 26.792198926912164 23.06952191220067 66-67 23.472504785087985 26.464441633366224 26.77719582705407 23.28585775449172 68-69 23.528504210079507 26.570433410901956 26.567121943289614 23.333940435728927 70-71 23.619877817525868 26.67167004891694 26.512070584417323 23.196381549139865 72-73 23.601840389585053 26.6144201381948 26.341290934007045 23.4424485382131 74-75 23.627014447725966 26.697103530495568 26.443974795507025 23.23190722627144 76-77 23.69646160070903 26.831215560094023 26.30371902816847 23.168603811028476 78-79 23.68063088943254 26.70949803667394 26.233007449455286 23.376863624438236 80-81 23.667617014993088 26.762989971143348 26.25869473889555 23.31069827496802 82-83 23.788665678937416 26.8762302399586 26.106563070998046 23.228541010105936 84-85 23.854668717769638 26.782653328476513 26.226989063125604 23.135688890628245 86-87 23.953636443002114 26.66416299645673 26.25382700291108 23.128373557630074 88-89 23.921756041170575 26.852690868477534 26.092934833327813 23.132618257024074 90-91 24.01709620415499 26.816355037494844 26.000665303947567 23.165883454402596 92-93 23.967574711224973 26.668844207490537 26.22626656110109 23.137314520183395 94-95 24.086471450633546 26.88082329105693 25.981368479042928 23.051336779266602 96-97 24.15071392231297 26.882433868486572 25.931019119209825 23.035833089990636 98-99 24.192212632345814 26.8731467070465 25.980209465378607 22.954431195229077 100-101 24.37977716833394 27.065603183825587 25.57508150725964 22.979538140580832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3443.0 1 2301.0 2 897.5 3 555.5 4 402.0 5 289.5 6 231.5 7 177.5 8 136.0 9 121.5 10 103.0 11 100.0 12 108.0 13 116.0 14 140.0 15 200.5 16 1984.0 17 2130.0 18 609.0 19 789.0 20 1052.0 21 1498.0 22 2204.0 23 3033.5 24 3955.0 25 5359.0 26 7268.5 27 9550.5 28 12001.0 29 15176.5 30 20135.5 31 26025.5 32 32726.0 33 41592.0 34 51085.5 35 60806.0 36 70145.0 37 78534.5 38 87398.5 39 98006.0 40 105686.5 41 108766.0 42 111673.5 43 116607.5 44 123698.0 45 130943.0 46 135432.0 47 137547.5 48 138714.5 49 138590.5 50 137047.0 51 136410.0 52 140312.5 53 141723.5 54 133151.5 55 122657.5 56 113158.5 57 98510.0 58 83413.5 59 70843.0 60 59029.0 61 48064.5 62 37472.5 63 28785.0 64 21761.5 65 15613.5 66 11326.5 67 8405.0 68 6287.0 69 4739.0 70 3715.0 71 2875.5 72 2313.5 73 1808.5 74 1309.5 75 974.0 76 700.0 77 438.0 78 264.5 79 161.5 80 80.0 81 39.0 82 20.5 83 13.0 84 8.5 85 2.0 86 1.5 87 3.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 1.5 95 2.0 96 1.0 97 0.5 98 1.0 99 3.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08504450913513496 2 0.04307918321146129 3 0.049310763172867644 4 0.055903594146529435 5 0.04055042612567321 6 0.016707859316814126 7 0.020741828953666545 8 0.011198781379918657 9 0.0024986528347667974 10-11 9.332317816598883E-4 12-13 5.418765183831609E-4 14-15 0.0011891179153408252 16-17 7.52606275532168E-5 18-19 0.0018815156888304196 20-21 0.005644547066491259 22-23 0.015443480773920087 24-25 0.01904093877096385 26-27 0.010385966602343916 28-29 0.01783676873011238 30-31 3.7630313776608395E-4 32-33 0.00591548532568284 34-35 2.2578188265965035E-4 36-37 0.0026792783408945177 38-39 0.009618308201301105 40-41 0.0018815156888304196 42-43 0.0018664635633197765 44-45 0.002272870952107147 46-47 0.0 48-49 0.0 50-51 0.003747979252150196 52-53 0.002483600709256154 54-55 0.0025438092112987274 56-57 0.0 58-59 0.004636054657278154 60-61 0.0 62-63 0.0 64-65 0.0020320369439368532 66-67 3.612510122554406E-4 68-69 0.0 70-71 0.0011138572877876086 72-73 0.003070633604171245 74-75 0.004816680163405875 76-77 0.0018664635633197765 78-79 1.0536487857450352E-4 80-81 7.676584010428112E-4 82-83 8.880754051279582E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3321790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02081080927984 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10949084894723 54.026012516352495 2 7.76666699560426 9.633899687060993 3 1.8816596464400082 3.501061708179364 4 0.7584146426163758 1.8814996425879131 5 0.4347639733458291 1.3482207068786225 6 0.28743614484036 1.0696213655335636 7 0.21755551113328778 0.9445078437559672 8 0.1610585398627897 0.7991184984039149 9 0.12885960967389573 0.71927797252881 >10 1.0359804456897796 13.497679173739144 >50 0.1634392984229251 7.01287704529447 >100 0.054137136062840485 4.8849608701696985 >500 3.418592296324505E-4 0.15405048315251837 >1k 1.4651109810301445E-4 0.22332170871831517 >5k 0.0 0.0 >10k+ 4.8837032701004814E-5 0.303890777644223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 10033 0.3020359504965696 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3762 0.11325219234208063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.020850204257343E-5 0.0 0.0 0.0 0.0 2 6.020850204257343E-5 0.0 0.0 0.0 0.0 3 6.020850204257343E-5 0.0 0.0 0.0 0.0 4 6.020850204257343E-5 0.0 0.0 0.0 0.0 5 9.031275306386014E-5 0.0 0.0 0.0 0.0 6 2.10729757149007E-4 0.0 0.0 0.0 0.0 7 2.4083400817029372E-4 0.0 0.0 0.0 0.0 8 3.612510122554406E-4 0.0 0.0 0.0 0.0 9 9.031275306386015E-4 0.0 0.0 0.0 0.0 10-11 0.0015804731786175526 0.0 0.0 0.0 0.0 12-13 0.0027545389684477343 0.0 0.0 0.0 0.0 14-15 0.003326519737852182 0.0 0.0 0.0 0.0 16-17 0.003913552632767273 0.0 0.0 0.0 0.0 18-19 0.004816680163405874 0.0 0.0 0.0 0.0 20-21 0.006818612856321441 0.0 0.0 0.0 0.0 22-23 0.008910858302300869 0.0 0.0 0.0 0.0 24-25 0.010461227229897133 0.0 0.0 0.0 0.0 26-27 0.012086856785046616 0.0 0.0 0.0 0.0 28-29 0.015052125510643357 0.0 0.0 0.0 0.0 30-31 0.017987289985218813 0.0 0.0 0.0 0.0 32-33 0.021238549095517777 0.0 0.0 0.0 0.0 34-35 0.025001580473178617 0.0 0.0 0.0 1.5052125510643358E-5 36-37 0.030465502033542157 0.0 0.0 0.0 6.020850204257343E-5 38-39 0.03838292005214056 0.0 0.0 0.0 6.020850204257343E-5 40-41 0.048889303658569624 0.0 0.0 0.0 6.020850204257343E-5 42-43 0.0629028325089786 0.0 0.0 0.0 6.020850204257343E-5 44-45 0.08104064374930384 0.0 0.0 0.0 6.020850204257343E-5 46-47 0.10236950559788548 0.0 0.0 0.0 7.526062755321678E-5 48-49 0.12961385277214996 0.0 0.0 0.0 9.031275306386014E-5 50-51 0.16131362909756486 0.0 0.0 0.0 1.2041700408514686E-4 52-53 0.19924498538438612 0.0 0.0 0.0 1.2041700408514686E-4 54-55 0.24313698337342216 0.0 0.0 0.0 1.2041700408514686E-4 56-57 0.29315519644529003 0.0 0.0 3.0104251021286716E-5 1.2041700408514686E-4 58-59 0.35110587966126694 0.0 0.0 3.0104251021286716E-5 1.2041700408514686E-4 60-61 0.419517790107141 0.0 0.0 4.515637653193007E-5 1.2041700408514686E-4 62-63 0.4983758756574016 0.0 0.0 6.020850204257343E-5 1.9567763163836363E-4 64-65 0.5873489895508145 0.0 0.0 6.020850204257343E-5 2.4083400817029372E-4 66-67 0.6846910852281451 0.0 0.0 1.2041700408514685E-4 2.5588613368093707E-4 68-69 0.7957456672456718 0.0 0.0 1.6557338061707694E-4 2.7093825919158044E-4 70-71 0.9164637138410314 0.0 0.0 1.806255061277203E-4 2.7093825919158044E-4 72-73 1.0480192908040546 0.0 0.0 1.806255061277203E-4 3.0104251021286714E-4 74-75 1.1973664801206578 0.0 0.0 1.806255061277203E-4 3.0104251021286714E-4 76-77 1.3630000692397775 0.0 0.0 1.806255061277203E-4 3.0104251021286714E-4 78-79 1.5431740116021784 0.0 0.0 1.806255061277203E-4 3.0104251021286714E-4 80-81 1.7409438886865214 0.0 0.0 1.806255061277203E-4 3.0104251021286714E-4 82-83 1.9548948006948061 0.0 0.0 1.806255061277203E-4 3.160946357235105E-4 84-85 2.183762369084138 0.0 0.0 1.806255061277203E-4 3.461988867447972E-4 86-87 2.4331008281679454 0.0 0.0 1.9567763163836363E-4 3.612510122554406E-4 88-89 2.7047314851330158 0.0 0.0 2.2578188265965038E-4 3.913552632767273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 13395 0.0 86.27949 1 TATCTCG 6780 0.0 83.20365 2 ATATCTC 6960 0.0 81.283615 1 ATCTCGA 6985 0.0 80.76174 3 TCGACGG 14655 0.0 78.86769 2 CGACGGC 14775 0.0 78.227135 3 TCTCGAG 5420 0.0 77.42411 4 CGGCGCG 15305 0.0 75.4909 6 TCGAGGG 5565 0.0 75.209915 6 CTCGAGG 5610 0.0 74.70256 5 AGGGCGC 5605 0.0 74.483475 9 CGAGGGC 5635 0.0 74.02048 7 GACGGCG 15745 0.0 73.37651 4 ACGGCGC 15765 0.0 73.26908 5 GAGGGCG 6140 0.0 68.46274 8 CTCGACG 2120 0.0 67.68631 5 CGCGCCG 20395 0.0 56.891376 9 GGCGCGC 20660 0.0 56.221165 7 GCGCGCC 20670 0.0 56.138702 8 TCTCGAC 2600 0.0 55.201183 4 >>END_MODULE