FastQCFastQC Report
Tue 31 May 2016
SRR2048244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321790
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT151380.45571815196023835No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT141450.42582463069610066No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT39920.12017617007697658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34090.10262539173156641No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.10244476622543869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC33200.068.413851
TATCTCG36100.062.5231132
TCGACGG37550.062.386582
GTCGACG36850.062.1532331
CGACGGC39550.059.1116143
CGGCGCG48550.048.232016
ACGGCGC50650.046.04475
GACGGCG50800.046.020954
ATCTCGA59750.037.775473
TCTCGAG59100.035.859284
TCGAGGG63800.032.9063156
CTCGAGG64650.032.841025
CGAGGGC65800.032.0504957
AGGGCGC69350.030.6838049
GGTCGAC3450.030.3010541
GAGGGCG71150.029.6405128
GGGCGCG68300.028.9301228
CGCGCCG115700.024.0570229
GCGCGCC118450.023.4584058
GGCGCGC118800.023.4292747
Produced by FastQC (version 0.11.5)