##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048244_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3321790 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28519593351777 33.0 31.0 34.0 30.0 34.0 2 32.415828213101975 34.0 31.0 34.0 31.0 34.0 3 32.49339482628342 34.0 31.0 34.0 31.0 34.0 4 36.05507060952077 37.0 35.0 37.0 35.0 37.0 5 35.91572736386105 37.0 35.0 37.0 35.0 37.0 6 35.90345626905975 37.0 35.0 37.0 35.0 37.0 7 35.88088861728165 37.0 35.0 37.0 35.0 37.0 8 35.85027018565292 37.0 35.0 37.0 35.0 37.0 9 37.446552912736806 39.0 37.0 39.0 34.0 39.0 10-11 37.443584784107365 39.0 37.0 39.0 34.0 39.0 12-13 37.44397011852044 39.0 37.0 39.0 34.0 39.0 14-15 38.78374415601227 40.0 38.0 41.0 34.5 41.0 16-17 38.796964136805755 40.0 38.0 41.0 34.5 41.0 18-19 38.82964817161832 40.0 38.0 41.0 35.0 41.0 20-21 38.84984571571351 40.0 38.0 41.0 35.0 41.0 22-23 38.826984396966694 40.0 38.0 41.0 35.0 41.0 24-25 38.813666426836136 40.0 38.0 41.0 35.0 41.0 26-27 38.726435144906816 40.0 38.0 41.0 34.0 41.0 28-29 38.63704704391307 40.0 38.0 41.0 34.0 41.0 30-31 38.5056639643084 40.0 38.0 41.0 34.0 41.0 32-33 38.40106072328473 40.0 38.0 41.0 34.0 41.0 34-35 38.35547280231442 40.0 38.0 41.0 34.0 41.0 36-37 38.273130300229695 40.0 38.0 41.0 34.0 41.0 38-39 38.17980275694731 40.0 37.0 41.0 33.5 41.0 40-41 38.04433513256407 40.0 37.0 41.0 33.0 41.0 42-43 37.96515929664428 40.0 37.0 41.0 33.0 41.0 44-45 37.839250675087825 40.0 37.0 41.0 33.0 41.0 46-47 37.71632553532884 40.0 37.0 41.0 33.0 41.0 48-49 37.596279716658785 40.0 36.0 41.0 32.5 41.0 50-51 37.495066214300124 39.0 36.0 41.0 32.0 41.0 52-53 37.450490097206625 39.0 36.0 41.0 32.0 41.0 54-55 37.2552733616514 39.0 35.5 41.0 32.0 41.0 56-57 36.99906451039951 39.0 35.0 41.0 31.0 41.0 58-59 36.73635931229849 39.0 35.0 41.0 31.0 41.0 60-61 36.4571687252957 38.0 35.0 40.0 31.0 41.0 62-63 36.13291779432174 37.5 35.0 40.0 31.0 41.0 64-65 35.815739104518954 37.0 35.0 40.0 30.5 41.0 66-67 35.477371989198595 36.5 34.5 39.5 30.0 41.0 68-69 35.120525529910076 36.0 34.0 39.0 30.0 41.0 70-71 34.7668738842612 36.0 34.0 39.0 29.5 40.5 72-73 34.40478898425246 35.0 34.0 37.5 29.0 40.0 74-75 34.07350133512353 35.0 34.0 37.0 29.0 39.0 76-77 33.839994250088054 35.0 34.0 37.0 29.0 39.0 78-79 33.70563536526993 35.0 34.0 36.0 29.0 38.5 80-81 33.470717595031594 35.0 34.0 36.0 29.0 37.0 82-83 33.250052983481794 35.0 34.0 35.5 29.0 37.0 84-85 33.0601370044464 35.0 34.0 35.0 29.0 36.5 86-87 32.8760985793804 35.0 33.5 35.0 29.0 36.0 88-89 32.74850517341554 35.0 33.0 35.0 29.0 36.0 90-91 32.629164546825656 35.0 33.0 35.0 29.0 36.0 92-93 32.501215760177494 35.0 33.0 35.0 29.0 35.0 94-95 32.3955739224936 35.0 33.0 35.0 29.0 35.0 96-97 32.286622272931154 35.0 33.0 35.0 29.0 35.0 98-99 32.14311019059001 35.0 33.0 35.0 29.0 35.0 100-101 31.689695465396667 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3709027900141848 1101 2 0.3519096534739887 1101 3 0.3368914787041888 1101 4 0.2406153499115007 1101 5 0.23966988006321088 1101 6 0.30866932736682884 1101 7 0.27661952670545276 1101 8 0.28355406437081143 1101 9 0.4145738483346193 1101 10-11 0.4478674264738842 1101 12-13 0.39603502318943384 1101 14-15 0.4699421064311693 1101 16-17 0.4601655507207454 1101 18-19 0.5082206844402606 1101 20-21 0.45851045501443366 1101 22-23 0.5388571649280891 1101 24-25 0.48668516678949203 1101 26-27 0.5139585464128515 1101 28-29 0.4737503893760149 1101 30-31 0.47426810135065267 1101 32-33 0.5340344050038226 1101 34-35 0.5293318945993235 1101 36-37 0.5258997148269771 1101 38-39 0.4763743206039237 1101 40-41 0.4919707152241841 1101 42-43 0.4486570243063568 1101 44-45 0.5362107041119799 1101 46-47 0.5677038155457268 1101 48-49 0.5236958316010103 1101 50-51 0.5443619847905055 1101 52-53 0.4513323195382668 1101 54-55 0.4810966811297135 1101 56-57 0.44023146682448555 1101 58-59 0.43393576103994747 1101 60-61 0.3926409496017911 1101 62-63 0.387271132592403 1101 64-65 0.33120532768204214 1101 66-67 0.34917563417617004 1101 68-69 0.2694441564613257 1101 70-71 0.27918359078039856 1101 72-73 0.27081643029992364 1101 74-75 0.23897259600341414 1101 76-77 0.26181910991368085 1101 78-79 0.25637235555888793 1101 80-81 0.23969738487213732 1101 82-83 0.29920809833086537 1101 84-85 0.268801727567471 1101 86-87 0.29137310272620454 1101 88-89 0.22345108732298513 1101 90-91 0.3225400383030177 1101 92-93 0.35182996728158145 1101 94-95 0.3205876056782415 1101 96-97 0.3816435444425821 1101 98-99 0.40089464269412645 1101 100-101 0.38579929608144425 1102 1 0.3549423128868341 1102 2 0.3375186486030799 1102 3 0.3584370585915906 1102 4 0.24154324078598677 1102 5 0.21619286655582926 1102 6 0.27243816249342956 1102 7 0.2777237764432243 1102 8 0.2975798239545142 1102 9 0.34627784469944345 1102 10-11 0.371665498251204 1102 12-13 0.4072769277094821 1102 14-15 0.5176178060771832 1102 16-17 0.5380039675679029 1102 18-19 0.5313432166324503 1102 20-21 0.5491035097348202 1102 22-23 0.6028064699038893 1102 24-25 0.537091753026786 1102 26-27 0.5391703838143513 1102 28-29 0.553344185046889 1102 30-31 0.5744085559819112 1102 32-33 0.576298989030434 1102 34-35 0.5605909557960942 1102 36-37 0.5300901838588459 1102 38-39 0.5680460834336074 1102 40-41 0.5154292414576247 1102 42-43 0.5361704275799681 1102 44-45 0.6035755937624216 1102 46-47 0.4919411425194369 1102 48-49 0.5735513876106353 1102 50-51 0.584105245206139 1102 52-53 0.5066203366711051 1102 54-55 0.4805027166408493 1102 56-57 0.5094689227226041 1102 58-59 0.4369176245376991 1102 60-61 0.4325511428035398 1102 62-63 0.40989596790970495 1102 64-65 0.3962303000970806 1102 66-67 0.4322237526581887 1102 68-69 0.3295092486933626 1102 70-71 0.36653705480902943 1102 72-73 0.3600782188561027 1102 74-75 0.33036297522605196 1102 76-77 0.308476063463921 1102 78-79 0.2882774983292862 1102 80-81 0.22456999052690918 1102 82-83 0.23243796374351433 1102 84-85 0.21002064948694965 1102 86-87 0.20613734376523496 1102 88-89 0.28410388026357225 1102 90-91 0.24278143831507748 1102 92-93 0.2628749823341181 1102 94-95 0.17096432037289588 1102 96-97 0.227094434458877 1102 98-99 0.3322996803789735 1102 100-101 0.3080766071366412 1103 1 0.2800265110839888 1103 2 0.29609679270355116 1103 3 0.24114252007844783 1103 4 0.17658821610257291 1103 5 0.2322432521798703 1103 6 0.2260382561790948 1103 7 0.2268051752614042 1103 8 0.2264240789226264 1103 9 0.28873030291909174 1103 10-11 0.3124972223612943 1103 12-13 0.26925135719712756 1103 14-15 0.33527117989040534 1103 16-17 0.33984582623723725 1103 18-19 0.418553863705057 1103 20-21 0.433799172196224 1103 22-23 0.4544595083831666 1103 24-25 0.41168559027631346 1103 26-27 0.4531011711276989 1103 28-29 0.42848013218642933 1103 30-31 0.3763514145179414 1103 32-33 0.3990412453242911 1103 34-35 0.3756553836790104 1103 36-37 0.3805461920619351 1103 38-39 0.4513931758504768 1103 40-41 0.43059243098305444 1103 42-43 0.388474949172533 1103 44-45 0.3735029334111388 1103 46-47 0.3894847913884263 1103 48-49 0.4686400442704368 1103 50-51 0.4629055450482369 1103 52-53 0.4592178458073519 1103 54-55 0.4778469037627886 1103 56-57 0.4493694856160033 1103 58-59 0.31512877181345544 1103 60-61 0.3810045198941552 1103 62-63 0.41617174870522433 1103 64-65 0.3945777911976336 1103 66-67 0.37690454291919195 1103 68-69 0.36861861931870976 1103 70-71 0.33293015852054 1103 72-73 0.41685707051519927 1103 74-75 0.27163996606152097 1103 76-77 0.30120884927166003 1103 78-79 0.3208075290029555 1103 80-81 0.32575073372525765 1103 82-83 0.21549146909237038 1103 84-85 0.2655542491289964 1103 86-87 0.2761759151626819 1103 88-89 0.2591899669623814 1103 90-91 0.2845118494069183 1103 92-93 0.2871393909523121 1103 94-95 0.2950756504436214 1103 96-97 0.3553414232979293 1103 98-99 0.3998549220502312 1103 100-101 0.48108520211737726 1104 1 0.2000045417668872 1104 2 0.2046765329349114 1104 3 0.1824165453607236 1104 4 0.139573623871442 1104 5 0.18051331479047406 1104 6 0.16307372511475648 1104 7 0.16025297276907935 1104 8 0.20268765609716866 1104 9 0.28270181304624487 1104 10-11 0.26994415748547596 1104 12-13 0.2640661300995504 1104 14-15 0.35008681127413865 1104 16-17 0.3013538657195767 1104 18-19 0.35759437426730756 1104 20-21 0.32121022670393273 1104 22-23 0.3675162286333631 1104 24-25 0.3134515253478156 1104 26-27 0.33589935192776466 1104 28-29 0.31456366241764755 1104 30-31 0.3149170288296048 1104 32-33 0.31349485123844545 1104 34-35 0.256781086804466 1104 36-37 0.34074397444863536 1104 38-39 0.2855966320181835 1104 40-41 0.3288776145712333 1104 42-43 0.29255058918010235 1104 44-45 0.33048706906679826 1104 46-47 0.33243451365021315 1104 48-49 0.22706428231950326 1104 50-51 0.30361980149611867 1104 52-53 0.3096758650958691 1104 54-55 0.3513623652062492 1104 56-57 0.2904169888987198 1104 58-59 0.1980323116830789 1104 60-61 0.1845286832163211 1104 62-63 0.17063838792650188 1104 64-65 0.15319050491558528 1104 66-67 0.15867244859551022 1104 68-69 0.18572746889522307 1104 70-71 0.08131826231092987 1104 72-73 0.0779975239103905 1104 74-75 0.09252613339123883 1104 76-77 0.12435477353410107 1104 78-79 0.10317822513373898 1104 80-81 0.08579603812516723 1104 82-83 0.13897200595535963 1104 84-85 0.1459164892189051 1104 86-87 0.18920941739570907 1104 88-89 0.09293169676318769 1104 90-91 0.1065300414062591 1104 92-93 0.11068099705377676 1104 94-95 0.08899545658293562 1104 96-97 0.08480703819492419 1104 98-99 0.1703964286459012 1104 100-101 0.12708692298218693 1105 1 0.1566066735093301 1105 2 0.23942583470376633 1105 3 0.19209374407635949 1105 4 0.12115742110891148 1105 5 0.1525582295777781 1105 6 0.1924233726742699 1105 7 0.23112599919736 1105 8 0.22363497051967585 1105 9 0.23509973830226016 1105 10-11 0.18672260446741262 1105 12-13 0.1923335668787587 1105 14-15 0.256677543324372 1105 16-17 0.2161081073550548 1105 18-19 0.2779091957671227 1105 20-21 0.24549670096913445 1105 22-23 0.3060173591028459 1105 24-25 0.25043516678949373 1105 26-27 0.2861227858950457 1105 28-29 0.19661446704592578 1105 30-31 0.19625839261278344 1105 32-33 0.2504583532238911 1105 34-35 0.24266522793266176 1105 36-37 0.2899328863480122 1105 38-39 0.3326405018337013 1105 40-41 0.2573671553536272 1105 42-43 0.23011333498596542 1105 44-45 0.30257397272038844 1105 46-47 0.21504200324798717 1105 48-49 0.30672172156864264 1105 50-51 0.280349039806687 1105 52-53 0.2422141965609157 1105 54-55 0.19154571025593015 1105 56-57 0.2014167419053905 1105 58-59 0.1869090620108267 1105 60-61 0.15574370041085928 1105 62-63 0.0800988024953142 1105 64-65 0.08800953480177753 1105 66-67 0.2058827539172654 1105 68-69 0.25164884901796825 1105 70-71 0.3046123933693963 1105 72-73 0.23030672156205156 1105 74-75 0.13628651186101592 1105 76-77 0.1951888510140023 1105 78-79 0.19494840733882057 1105 80-81 0.22108492532521495 1105 82-83 0.14752120512698497 1105 84-85 0.15073133921795545 1105 86-87 0.17390951049319625 1105 88-89 0.15161047243624637 1105 90-91 0.10128599299557095 1105 92-93 0.07526443330099397 1105 94-95 0.03614990341286983 1105 96-97 0.10396555091507764 1105 98-99 0.09276357473295604 1105 100-101 0.04717470061218876 1106 1 0.09390630719516935 1106 2 0.10648618708103896 1106 3 0.10393783926154043 1106 4 0.05910342508281019 1106 5 0.05859968240423541 1106 6 0.0902889570348222 1106 7 0.1212123514051342 1106 8 0.11900453348521722 1106 9 0.15490462186332365 1106 10-11 0.11414107837811827 1106 12-13 0.1636829025223534 1106 14-15 0.2096886645847107 1106 16-17 0.2083339602832126 1106 18-19 0.21145208465810583 1106 20-21 0.21192744499180804 1106 22-23 0.26562552536616124 1106 24-25 0.25957644728821094 1106 26-27 0.14972952080224644 1106 28-29 0.194438899918147 1106 30-31 0.1802461319176345 1106 32-33 0.21160974738358362 1106 34-35 0.08068336472133808 1106 36-37 0.20606662397666753 1106 38-39 0.16055985621675717 1106 40-41 0.2525194723613424 1106 42-43 0.19529145407103243 1106 44-45 0.13433595326525705 1106 46-47 0.10772202923840268 1106 48-49 0.09691448380344525 1106 50-51 0.10810331739560297 1106 52-53 -0.05594848836182109 1106 54-55 0.013122744523720087 1106 56-57 0.05174915552963455 1106 58-59 0.046430405225535765 1106 60-61 -0.10791030691542858 1106 62-63 -0.07861015195554444 1106 64-65 -0.07679586668419347 1106 66-67 0.011514292719262187 1106 68-69 -0.05822364728594209 1106 70-71 -0.0810075627482405 1106 72-73 -0.05876214361737908 1106 74-75 -0.058970143474077474 1106 76-77 -0.030163667513583903 1106 78-79 0.010659989081496235 1106 80-81 -0.07687030712889964 1106 82-83 -0.054608125431528265 1106 84-85 -0.04801735419874831 1106 86-87 -0.05475640910601953 1106 88-89 -0.06604596442870303 1106 90-91 -0.07507911581573978 1106 92-93 -0.06573892850251184 1106 94-95 -0.049617331715623436 1106 96-97 -0.004560152285698393 1106 98-99 -0.014055142131617515 1106 100-101 -0.07118978668923859 1107 1 0.02288733530800613 1107 2 0.09069143056400719 1107 3 0.09744242787414947 1107 4 0.08642857948471772 1107 5 0.09872315455225333 1107 6 0.14803822455280624 1107 7 0.15656201791144753 1107 8 0.12243684344870331 1107 9 0.21224681037733006 1107 10-11 0.03568614394586689 1107 12-13 0.12134666711620667 1107 14-15 0.16777172017282282 1107 16-17 0.07192390178150276 1107 18-19 0.02786868407652321 1107 20-21 0.04180095498180236 1107 22-23 0.12414760485349063 1107 24-25 0.11782103161237956 1107 26-27 0.038702112575919045 1107 28-29 0.0891637949244668 1107 30-31 0.1642379409578112 1107 32-33 0.15540749023553246 1107 34-35 0.08835583724292917 1107 36-37 0.0718680752473162 1107 38-39 0.12099986391239526 1107 40-41 0.20318903318739956 1107 42-43 0.20889901084301243 1107 44-45 0.2231919708611585 1107 46-47 0.22722457770794335 1107 48-49 0.13189976579121065 1107 50-51 0.17877661208991924 1107 52-53 0.21604351136014088 1107 54-55 0.09609345644857115 1107 56-57 0.15372007200057425 1107 58-59 0.0479116645685167 1107 60-61 0.18726379181405406 1107 62-63 -0.016759611902593008 1107 64-65 -0.0034692464636094655 1107 66-67 -0.02295276785847733 1107 68-69 0.07302683582837233 1107 70-71 0.10110534707276031 1107 72-73 0.02452837811188857 1107 74-75 0.04964333601822801 1107 76-77 -0.03211618053416743 1107 78-79 -0.11085569553920749 1107 80-81 -0.05260817818206931 1107 82-83 -0.03840015542706254 1107 84-85 -0.0017993392150330578 1107 86-87 -0.15517875784590274 1107 88-89 -0.1129552578292703 1107 90-91 -0.0842447747874644 1107 92-93 -0.04227971417600429 1107 94-95 -0.02164942399041081 1107 96-97 -0.005537092515510267 1107 98-99 -0.07751365754484141 1107 100-101 -0.09389582699032317 1108 1 0.01742813905310925 1108 2 0.022837416174155578 1108 3 0.062241566838267204 1108 4 0.044159948551723005 1108 5 0.020585238708278553 1108 6 0.08626928260031264 1108 7 0.03695627814870761 1108 8 0.010941611818651609 1108 9 -0.14635349467946668 1108 10-11 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-0.2741257368869796 2214 86-87 -0.18307911196373539 2214 88-89 -0.17606821951714124 2214 90-91 -0.2719929867340056 2214 92-93 -0.2278112117849318 2214 94-95 -0.21349441857011442 2214 96-97 -0.24573651172816113 2214 98-99 -0.44873534610633925 2214 100-101 -0.6621655650616027 2215 1 -0.5426843118709108 2215 2 -0.8896883428025006 2215 3 -0.5488729319393286 2215 4 -0.2853244198178615 2215 5 -0.3026717655372053 2215 6 -0.3631046270136835 2215 7 -0.33713777682742574 2215 8 -0.46312540921140766 2215 9 -0.5174255551237721 2215 10-11 -0.541827158618581 2215 12-13 -0.42641337998600903 2215 14-15 -0.5131724885303939 2215 16-17 -0.4998219124636307 2215 18-19 -0.49931113704550967 2215 20-21 -0.4996654425686273 2215 22-23 -0.5387476121721448 2215 24-25 -0.4965135925463642 2215 26-27 -0.5470802246123867 2215 28-29 -0.5651823088432693 2215 30-31 -0.5293137022419714 2215 32-33 -0.5984557986499652 2215 34-35 -0.591360824249719 2215 36-37 -0.5930001480644265 2215 38-39 -0.570107960686336 2215 40-41 -0.6049712815525794 2215 42-43 -0.5179265846991612 2215 44-45 -0.4911531461786751 2215 46-47 -0.5222663527303979 2215 48-49 -0.6142017879905737 2215 50-51 -0.5153217398084919 2215 52-53 -0.5754328415032077 2215 54-55 -0.6390385599913557 2215 56-57 -0.5390578476510797 2215 58-59 -0.52112214637134 2215 60-61 -0.4860777420248965 2215 62-63 -0.4540752489663902 2215 64-65 -0.4359825080080526 2215 66-67 -0.4087966088388413 2215 68-69 -0.4311802812819536 2215 70-71 -0.38875119115532186 2215 72-73 -0.4261903130658382 2215 74-75 -0.4555711688520816 2215 76-77 -0.3945299912185405 2215 78-79 -0.2924066070469067 2215 80-81 -0.26203580144635197 2215 82-83 -0.41320983850197734 2215 84-85 -0.3813449041858803 2215 86-87 -0.38801919798648754 2215 88-89 -0.35921630265425364 2215 90-91 -0.387024107100828 2215 92-93 -0.38546521726244976 2215 94-95 -0.38403442701483925 2215 96-97 -0.40139698973921867 2215 98-99 -0.37657208161449773 2215 100-101 -0.4997932644534444 2216 1 -0.5054246643472489 2216 2 -0.4798450728195718 2216 3 -0.48969431305150835 2216 4 -0.3233078485990717 2216 5 -0.325267612908263 2216 6 -0.32070741414583637 2216 7 -0.3670649518091196 2216 8 -0.45092476258374603 2216 9 -0.5275324917086124 2216 10-11 -0.6502361989962537 2216 12-13 -0.7185091065852731 2216 14-15 -0.9241846225863384 2216 16-17 -1.2530915777669662 2216 18-19 -1.2852217784803557 2216 20-21 -1.5793676016136189 2216 22-23 -1.4157624512206581 2216 24-25 -1.4434374575685638 2216 26-27 -1.2717234861745226 2216 28-29 -1.3330747783714756 2216 30-31 -1.2603553570592183 2216 32-33 -1.1047708704519295 2216 34-35 -0.7203847776901569 2216 36-37 -1.0950060123287528 2216 38-39 -1.2700533917825254 2216 40-41 -1.2924279865288923 2216 42-43 -0.7609177029868235 2216 44-45 -1.2733144330223851 2216 46-47 -0.8225847913690956 2216 48-49 -0.8431504529637266 2216 50-51 -0.9840448200726115 2216 52-53 -0.9947880900530208 2216 54-55 -1.142551264666288 2216 56-57 -1.2465984022262617 2216 58-59 -0.9470687091028651 2216 60-61 -1.0059007873501358 2216 62-63 -0.7871536996601094 2216 64-65 -0.9009732968509567 2216 66-67 -0.6094293126569283 2216 68-69 -0.7187867321943173 2216 70-71 -0.6218789662287634 2216 72-73 -0.490040768785434 2216 74-75 -0.4518965671958526 2216 76-77 -0.36738902505901905 2216 78-79 -0.2278393873907234 2216 80-81 -0.334369773478997 2216 82-83 -0.49801165089130706 2216 84-85 -0.3528974421292759 2216 86-87 -0.4166574573067834 2216 88-89 -0.4187782519330625 2216 90-91 -0.42013733046658785 2216 92-93 -0.3089506208655095 2216 94-95 -0.46979229398564115 2216 96-97 -0.4758027888532652 2216 98-99 -0.7293219296340965 2216 100-101 -0.4937545642976602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 34.0 13 138.0 14 500.0 15 1207.0 16 1949.0 17 3250.0 18 6100.0 19 12589.0 20 14427.0 21 13024.0 22 12244.0 23 11930.0 24 13038.0 25 15588.0 26 20107.0 27 26353.0 28 34790.0 29 44979.0 30 58723.0 31 76533.0 32 99947.0 33 131763.0 34 179277.0 35 255491.0 36 433000.0 37 809635.0 38 874668.0 39 170326.0 40 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.008868712350868 39.70181739363415 12.847260061593296 21.442053832421674 2 24.84142585774537 27.4432158565111 29.804773932126956 17.910584353616574 3 22.819895297414945 27.000954304757375 24.44299609547864 25.736154302349036 4 24.052291990921663 26.163011319009094 25.702378485691018 24.082318204378225 5 24.19794749216537 26.594667332974087 24.860963516658188 24.346421658202356 6 24.17272012980953 25.344227058302906 25.899921427904832 24.58313138398273 7 24.775347026753646 26.68479946053182 24.302439347460254 24.237414165254275 8 24.775437339506713 25.06172876671915 25.986651775097165 24.176182118676977 9 23.795965428278127 25.143491912492966 26.290463876403987 24.77007878282492 10-11 25.044524187260482 25.431574542641165 25.154449859864712 24.36945141023364 12-13 24.59264432730546 25.56966274207581 25.1572646073352 24.680428323283532 14-15 24.34232748006346 25.536834056337092 25.334307707591393 24.78653075600806 16-17 24.494971084866894 25.762977190009 25.281835998061286 24.460215727062817 18-19 23.947058664153964 25.817315363102423 25.94792265615828 24.287703316585336 20-21 23.36321681984713 26.177181579810888 26.09618609243811 24.36341550790387 22-23 23.10229170150607 25.961097173677018 26.828581401681234 24.108029723135676 24-25 23.016159961948226 25.497051288612465 27.899174842479507 23.5876139069598 26-27 22.9165402914725 25.381853781479464 28.48423371927502 23.217372207773014 28-29 22.99186687400496 25.51915227518454 28.44878153631533 23.040199314495172 30-31 23.046659037244837 25.52955267033025 28.3644704157202 23.05931787670471 32-33 22.92556466928443 25.5703740408596 28.469609528237715 23.03445176161825 34-35 23.083111816219567 25.680130291198424 28.343363066298593 22.89339482628342 36-37 23.029002891363223 25.709244971723827 28.18127096855475 23.0804811683582 38-39 23.04434676942663 25.679946619085843 28.280384666504006 22.99532194498352 40-41 23.10726819849322 25.86751524631629 27.805448635161927 23.219767920028563 42-43 23.21680022674532 25.999156779548127 27.55622460611204 23.227818387594514 44-45 23.178845926326872 25.941189380034384 27.687586869814716 23.192377823824025 46-47 23.296779219019882 26.271051354127863 27.35823709373342 23.07393233311884 48-49 23.297954310470306 26.261763426008784 27.21522540787127 23.225056855649644 50-51 23.238124539592693 26.149178138152745 27.313024585862973 23.299672736391585 52-53 23.305122822967693 26.38965425629995 27.12283651476141 23.182386405970945 54-55 23.305488606320637 26.35231266373127 26.99847731048115 23.343721419466945 56-57 23.35396228687115 26.338484927166473 26.98974573177845 23.317807054183927 58-59 23.483184290184827 26.529143567612806 26.718863127663322 23.268809014539045 60-61 23.3844626395414 26.476409894231217 26.720360867416026 23.41876659881136 62-63 23.391794996534987 26.37035045689387 26.815548781405617 23.42230576516552 64-65 23.44260244534299 26.62404097076164 26.6309800142002 23.302376569695166 66-67 23.44145236305825 26.48722306505852 26.585919956023663 23.48540461585957 68-69 23.490356570496537 26.58423469780188 26.399574082428057 23.525834649273527 70-71 23.530692973926552 26.629522814644 26.450612173630322 23.389172037799124 72-73 23.44095211402778 26.523524837685258 26.36692238244054 23.668600665846427 74-75 23.513124352000926 26.553888235287033 26.37738563004476 23.55560178266728 76-77 23.53704060498049 26.745204349501467 26.25045156784355 23.467303477674488 78-79 23.50949865810099 26.679541270732454 26.132154850479488 23.67880522068707 80-81 23.474519302701044 26.71528805712825 26.189110747250954 23.621081892919747 82-83 23.5255849071145 26.84375643491151 26.093603803326225 23.537054854647767 84-85 23.524696022325312 26.738369975224202 26.16860186826982 23.568332134180668 86-87 23.55579725927847 26.6637304982751 26.24121727159412 23.53925497085231 88-89 23.56500752458699 26.89751599821421 25.98539877902701 23.552077698171793 90-91 23.651058927265385 26.8136239544955 25.94908786143821 23.586229256800905 92-93 23.519014237573053 26.713391699736345 26.119386809578256 23.648207253112343 94-95 23.598180499068274 26.939466372046393 25.888903272031044 23.573449856854285 96-97 23.673439897765576 26.845913069132415 25.836528786169456 23.644118246932553 98-99 23.620660433278573 26.827306008900624 25.919090586427977 23.632942971392826 100-101 23.647448744035625 27.022423510642735 25.65647089568045 23.67365684964119 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2497.0 1 1668.5 2 619.5 3 330.0 4 225.5 5 174.5 6 167.0 7 163.5 8 163.0 9 160.0 10 147.5 11 154.5 12 183.5 13 333.5 14 431.0 15 451.5 16 2803.0 17 3097.5 18 1121.5 19 1231.5 20 1508.5 21 1984.0 22 2681.5 23 3487.5 24 4479.5 25 5892.0 26 7724.0 27 9834.5 28 11968.5 29 14955.5 30 19662.0 31 24920.5 32 30749.0 33 39019.0 34 47797.0 35 57056.5 36 66415.5 37 74663.0 38 83892.0 39 94963.5 40 102791.5 41 105957.0 42 108839.5 43 114429.0 44 122714.5 45 130373.5 46 135556.0 47 138715.5 48 140116.0 49 140632.0 50 139595.5 51 139291.0 52 143314.5 53 144813.5 54 136505.0 55 125874.5 56 116313.5 57 101131.0 58 85156.5 59 72197.0 60 59761.0 61 48103.5 62 37683.5 63 29107.0 64 22029.0 65 15782.5 66 11551.5 67 8664.0 68 6408.5 69 4808.5 70 3745.0 71 3010.5 72 2409.5 73 1843.0 74 1386.0 75 990.0 76 671.5 77 451.0 78 287.5 79 152.0 80 53.5 81 22.5 82 16.0 83 4.0 84 1.0 85 0.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.04088157288690736 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5052125510643358E-5 24-25 0.0 26-27 4.515637653193007E-5 28-29 1.3546912959579022E-4 30-31 1.5052125510643358E-5 32-33 1.5052125510643358E-5 34-35 0.0 36-37 1.5052125510643358E-5 38-39 1.0536487857450352E-4 40-41 1.2041700408514686E-4 42-43 4.515637653193007E-5 44-45 2.7093825919158044E-4 46-47 7.52606275532168E-5 48-49 1.5052125510643358E-5 50-51 9.031275306386014E-5 52-53 0.0011289094132982517 54-55 0.0010837530367663218 56-57 7.52606275532168E-5 58-59 0.0013546912959579022 60-61 4.8166801634058745E-4 62-63 3.612510122554406E-4 64-65 1.9567763163836366E-4 66-67 1.0536487857450352E-4 68-69 6.171371459363777E-4 70-71 6.020850204257344E-4 72-73 9.031275306386014E-5 74-75 7.827105265534546E-4 76-77 9.031275306386014E-4 78-79 1.505212551064336E-4 80-81 3.0104251021286716E-5 82-83 0.0019868805674049233 84-85 0.0 86-87 1.5052125510643358E-5 88-89 3.913552632767273E-4 90-91 2.558861336809371E-4 92-93 4.8166801634058745E-4 94-95 0.0 96-97 3.7630313776608395E-4 98-99 3.0104251021286716E-5 100-101 0.008986118929854084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3321790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.75671455378123 #Duplication Level Percentage of deduplicated Percentage of total 1 86.73669008939856 51.83099631011976 2 8.049402489460011 9.620116937823159 3 1.8510812793868174 3.3184360688449863 4 0.7668189288453041 1.8329031938178049 5 0.4200501638366309 1.2550408869327296 6 0.2962837868496757 1.062296740661366 7 0.2154691570236963 0.901301023798622 8 0.166469840399882 0.7958152587671414 9 0.13513703816175798 0.7267810873568052 >10 1.1130402437721543 14.045043964014232 >50 0.1843314423957112 7.599567213827604 >100 0.0646195589211468 5.5857842952136725 >500 1.0099692493519834E-4 0.03304296316730388 >1k 4.039876989606835E-4 0.5092237464616224 >5k 0.0 0.0 >10k+ 1.0099692474017088E-4 0.8836503091932261 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 15138 0.45571815196023835 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14145 0.42582463069610066 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 3992 0.12017617007697658 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3409 0.10262539173156641 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3403 0.10244476622543869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.0104251021286716E-5 0.0 0.0 0.0 0.0 2 3.0104251021286716E-5 0.0 0.0 0.0 0.0 3 3.0104251021286716E-5 0.0 0.0 0.0 0.0 4 3.0104251021286716E-5 0.0 0.0 0.0 3.0104251021286716E-5 5 6.020850204257343E-5 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 6 1.806255061277203E-4 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 7 1.806255061277203E-4 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 8 2.7093825919158044E-4 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 9 8.42919028596028E-4 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 10-11 0.0015052125510643358 0.0 0.0 3.0104251021286716E-5 3.0104251021286716E-5 12-13 0.0027244347174264476 0.0 0.0 3.0104251021286716E-5 6.020850204257343E-5 14-15 0.003281363361320252 0.0 0.0 3.0104251021286716E-5 6.020850204257343E-5 16-17 0.003868396256235343 1.5052125510643358E-5 0.0 3.0104251021286716E-5 6.020850204257343E-5 18-19 0.004756471661363301 3.0104251021286716E-5 0.0 3.0104251021286716E-5 6.020850204257343E-5 20-21 0.006683143726725651 3.0104251021286716E-5 0.0 3.0104251021286716E-5 6.020850204257343E-5 22-23 0.008654972168619931 3.0104251021286716E-5 0.0 3.0104251021286716E-5 9.031275306386014E-5 24-25 0.01008492409213105 3.0104251021286716E-5 0.0 3.0104251021286716E-5 9.031275306386014E-5 26-27 0.011605188768706028 3.0104251021286716E-5 0.0 3.0104251021286716E-5 9.031275306386014E-5 28-29 0.014510248992260198 3.0104251021286716E-5 0.0 3.0104251021286716E-5 1.053648785745035E-4 30-31 0.017340048588261145 3.0104251021286716E-5 0.0 4.515637653193007E-5 1.2041700408514686E-4 32-33 0.020606359824070757 3.0104251021286716E-5 0.0 7.526062755321678E-5 1.806255061277203E-4 34-35 0.024444651829284812 3.0104251021286716E-5 0.0 9.031275306386014E-5 1.806255061277203E-4 36-37 0.02984836488760578 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.10729757149007E-4 38-39 0.03769052227865097 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.10729757149007E-4 40-41 0.048166801634058745 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.10729757149007E-4 42-43 0.06224053898651028 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.10729757149007E-4 44-45 0.0803331938503036 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.2578188265965038E-4 46-47 0.1015416386948001 3.0104251021286716E-5 0.0 9.031275306386014E-5 2.4083400817029372E-4 48-49 0.12863546461395814 3.0104251021286716E-5 0.0 1.053648785745035E-4 2.4083400817029372E-4 50-51 0.16030513668835178 3.0104251021286716E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 52-53 0.19828164935170495 3.0104251021286716E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 54-55 0.24217364734074098 3.0104251021286716E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 56-57 0.2923875380442472 3.0104251021286716E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 58-59 0.35012749150307515 6.020850204257343E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 60-61 0.41844908919588536 6.020850204257343E-5 0.0 1.2041700408514686E-4 2.4083400817029372E-4 62-63 0.497216861993082 6.020850204257343E-5 0.0 1.806255061277203E-4 2.5588613368093707E-4 64-65 0.5858287248742395 6.020850204257343E-5 0.0 2.5588613368093707E-4 3.0104251021286714E-4 66-67 0.6829751429199318 6.020850204257343E-5 0.0 2.859903847022238E-4 3.0104251021286714E-4 68-69 0.793412587791522 6.020850204257343E-5 0.0 3.0104251021286714E-4 3.311467612341539E-4 70-71 0.9138446440021796 6.020850204257343E-5 0.0 3.0104251021286714E-4 3.612510122554406E-4 72-73 1.0446927710662024 6.020850204257343E-5 0.0 3.0104251021286714E-4 3.913552632767273E-4 74-75 1.1927755818399115 6.020850204257343E-5 0.0 3.0104251021286714E-4 3.913552632767273E-4 76-77 1.3580629720722863 6.020850204257343E-5 0.0 3.0104251021286714E-4 3.913552632767273E-4 78-79 1.5372133698999635 6.020850204257343E-5 0.0 3.0104251021286714E-4 4.064073887873707E-4 80-81 1.7332522525505827 6.020850204257343E-5 0.0 3.0104251021286714E-4 4.5156376531930076E-4 82-83 1.9454270137486116 6.020850204257343E-5 0.0 3.160946357235105E-4 4.5156376531930076E-4 84-85 2.172954942967497 6.020850204257343E-5 0.0 3.612510122554406E-4 4.5156376531930076E-4 86-87 2.4206828246216645 6.020850204257343E-5 0.0 3.612510122554406E-4 4.5156376531930076E-4 88-89 2.689589046869308 6.020850204257343E-5 0.0 3.612510122554406E-4 4.5156376531930076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 3320 0.0 68.41385 1 TATCTCG 3610 0.0 62.523113 2 TCGACGG 3755 0.0 62.38658 2 GTCGACG 3685 0.0 62.153233 1 CGACGGC 3955 0.0 59.111614 3 CGGCGCG 4855 0.0 48.23201 6 ACGGCGC 5065 0.0 46.0447 5 GACGGCG 5080 0.0 46.02095 4 ATCTCGA 5975 0.0 37.77547 3 TCTCGAG 5910 0.0 35.85928 4 TCGAGGG 6380 0.0 32.906315 6 CTCGAGG 6465 0.0 32.84102 5 CGAGGGC 6580 0.0 32.050495 7 AGGGCGC 6935 0.0 30.683804 9 GGTCGAC 345 0.0 30.301054 1 GAGGGCG 7115 0.0 29.640512 8 GGGCGCG 6830 0.0 28.930122 8 CGCGCCG 11570 0.0 24.057022 9 GCGCGCC 11845 0.0 23.458405 8 GGCGCGC 11880 0.0 23.429274 7 >>END_MODULE