FastQCFastQC Report
Tue 31 May 2016
SRR2048243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009936
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT125660.4174839597918361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34590.11491938699028817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG144700.088.1082841
TATCTCG84250.086.8683852
ATCTCGA86200.084.848143
CTCGACG28000.080.949395
ATATCTC90850.080.7360461
TCTCGAG62450.080.509994
CTCGAGG64300.078.2597055
TCGAGGG64900.077.516876
CGAGGGC65700.076.786077
TCGACGG166700.076.631022
AGGGCGC66450.075.905549
TCTCGAC30200.075.2172554
CGACGGC171400.074.585163
GAGGGCG69300.072.718978
CGGCGCG179500.071.112866
GACGGCG180250.071.078974
ACGGCGC180000.070.98585
CGCGCCG242550.052.81099
GGCGCGC246450.052.1388857
GCGCGCC247800.051.752118
Produced by FastQC (version 0.11.5)