FastQCFastQC Report
Tue 31 May 2016
SRR2048243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009936
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT187250.6221062507641358No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT134140.445657316301742No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT42700.14186348148266276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33400.11096581455552544No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30770.1022280872417221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42300.069.425461
TATCTCG43650.067.82262
TCGACGG44900.065.616952
ATATCTC46450.063.8365671
CGACGGC49700.059.2796973
GACGGCG60250.049.372824
ACGGCGC61350.048.776325
CGGCGCG62100.047.8812836
GGTCGAC3000.047.519371
ATCTCGA68200.043.4781343
TCTCGAG65300.042.279874
CTCGAGG71400.038.517975
TCGAGGG73700.037.4448176
CGAGGGC75300.036.5230187
GAGGGCG79400.034.5772448
AGGGCGC80150.034.3129549
GGGCGCG79600.026.1959728
CTCGACG8600.025.4173373
CGCGCCG140700.024.7115549
GGCGCGC145550.023.986027
Produced by FastQC (version 0.11.5)